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Fenotipe Virus Avian Influenza (AI) Subtipe H5N1 Berbeda Karakter Genetik di Indonesia Dharmayanti, NLP Indi; Indriani, Risa; Hartawan, Risza; Ratnawati, Atik
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.3031

Abstract

In Indonesia, data on the phenotype of AI virus subtype H5N1 is very limited, due to the facilities for such testingshould be performed in a laboratory Biosafety level III. Additionally influenza virus has a high error rate duringtranscription of their genome has low RNA polymerase. High error rates generate quasispecies. In this study, wedid some H5N1 viruses infection with different genetic variations and conducted DNA sequencing in severalorgans. To determine the possible emergence detection of quasispecies in different organs and until the virus wasexcreted. We tested the hypothesis that the quasispecies might arise from virus that was infected to theexperimental animals. The results of this study showed that the AI virus subtype H5N1 had different phenotypes inanimal depending on their genetic character. AI virus subtype H5N1 killed chickens within 48-72 hoursdepending on the genetic character of the virus. New AI viruses of subtype H5N1 caused more severe organdamage severe than the character of the old AI viruses. Adaptation of virus in each organ also proved that the viruslikely create variations/quasispecies, which was different from the viral origin.Keywords: Phenotypes, avian influenza, H5N1, genetic character, quasispecies
Status Infeksi Virus Hendra Pada Kalong (Pteropus spp.) di Pontianak, Kalimantan Barat dan Manado, Sulawesi Utara Sendow, Indrawati; Field, Hume; Ratnawati, Atik; Adjid, RM. Abdul; Saepulloh, Muharam; Breed, Andrew; Morrissy, Chris.; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i1.144

Abstract

Hendra merupakan salah satu penyakit emerging dan zoonosis yang berbahaya, termasuk Genus Henipavirus(Paramyxoviridae). Penyakit ini sangat erat hubungannya dengan Nipah, yang dapat menginfeksi ternak babi danmanusia. Survey serologi dilakukan di dua propinsi, yaitu Kalimantan Barat dan Sulawesi Utara. Hasil menunjukkanbahwa 148 kalong (Pteropus sp.) yang terdiri dari 84 P. vampyrus asal Kalimanatan Barat dan 64 P alecto asalSulawesi Utara telah dikoleksi. Hasil serologis mengindikasikan 22,6% P vampyrus di Kalimantan Barat mempunyaiantibodi terhadap virus Hendra, yang juga merupakan reaksi silang dengan virus Nipah. Di Sulawesi Utara, 25%serum mengandung antibodi terhadap virus Hendra, dimana 7,8% diantaranya hanya mempunyai antibodi terhadapvirus Hendra. Dari data tersebut dapat disimpulkan bahwa antibodi terhadap virus Hendra terdeteksi padaP. alecto di Sulawesi Utara. Hasil ini merupakan laporan pertama tentang infeksi Hendra pada P. alecto di Indonesia.Adanya perbedaan prevalensi tersebut, dapat disebabkan oleh letak geografis atau spesies kalong yang diuji.Kata kunci: P. vampyrus, P. alecto, antibodi, Hendra, Nipah, Serum Netralisasi
Virus Influenza Novel H1N1 Babi di Indonesia Hewajuli, Dyah Ayu; Dharmayanti, NLP Indi; Ratnawati, Atik
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (63.72 KB) | DOI: 10.14203/jbi.v7i2.3115

Abstract

ABSTRACTNovel H1N1 influenza virus in Swine in Indonesia. Novel H1N1 influenza virus occurred sinceApril 2009 has caused mortality in human population. In Indonesia, this situation requireintensive surveillance to prevent reassortant probability between the H5N1 virus and novelH1N1 virus. This study conduct preliminary surveillance of novel H1N1 virus circulation byusing Real Time-Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR), that validatedby CDC to detect novel H1N1 virus. Result of this study revealed that the influenza novelH1N1 virus was detected in swine/pigs in Indonesia especially in Bulan island and two individualsample from Kapok slaughter house in Jakarta. These findings showed that in Indonesia thenovel H1N1 virus is not only found in human but also has circulated in swine in Indonesia.Key words: pig/swine, influenza novel H1N1 virus
Sirkulasi virus Avian influenza H5N1 Tahun 2010 : Virus genetic drift mirip A/Ck/West Java/Pwt-Wij/2006 ditemukan di beberapa kabupaten di Sumatra dan Jawa Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2654.808 KB) | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
POTENSI TRANSMISI VIRUS AVIAN INFLUENZA DARI BABI DAN UNGGAS PADA PETERNAKAN BABI DI WILAYAH TANGERANG, PROVINSI BANTEN [The potential of Transmission of aAvian Influenza Virus from Pig and Bird at the Pig Farm in Tangerang District, Banten Province] Dharmayanti, NLP Indi; Ratnawati, Atik
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.724 KB) | DOI: 10.14203/beritabiologi.v11i2.489

Abstract

Pigs have an important role in the ecology of influenza virus since they are sensitive to influenza viruses from human and avian origin. Influenza A virus has a host specificity, although not absolute, so most of the AI virus circulating in various species is only limited to the species, but sometimes there are interactions between different AI virus species or strain. Farming systems that combine a variety of animal species together in the same or around the sites have an important role in the spread of disease and transmission between species. This study is aimed to investigate the cycle of AI virus in order to determine the potential occurrence of viral transmission among species pig and bird at the pig farm that also raising poultry. Influenza virus was identified by methods of RT-PCR and qRT-PCR. The results showed that the novel H1N1 pandemic virus was detected in one pig farm in Tangerang (Banten Province). The AI/H5 virus is also detected in the pig farm that also raises poultry or poultry/pig farmers and located adjacent each other. The AI virus / influence A is also detected in most of the pigs. Detection of AI viruses that infected in pig farm which kept birds or poultry farm around the pigs farm had potential of AI virus transmission from birds species to pig or vise versa. The pigs could serve as a mixing vessel, thus providing opportunities likelihood of reassortant viruses.
FENOTIPE VIRUS AVIAN INFLUENZA (AI) SUBTIPE H5N1 BERBEDA KARAKTER GENETIK DI INDONESIA Dharmayanti, NLP Indi; Indriani, Risa; Hartawan, Risza; Ratnawati, Atik
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.3031

Abstract

In Indonesia, data on the phenotype of AI virus subtype H5N1 is very limited, due to the facilities for such testingshould be performed in a laboratory Biosafety level III. Additionally influenza virus has a high error rate duringtranscription of their genome has low RNA polymerase. High error rates generate quasispecies. In this study, wedid some H5N1 viruses infection with different genetic variations and conducted DNA sequencing in severalorgans. To determine the possible emergence detection of quasispecies in different organs and until the virus wasexcreted. We tested the hypothesis that the quasispecies might arise from virus that was infected to theexperimental animals. The results of this study showed that the AI virus subtype H5N1 had different phenotypes inanimal depending on their genetic character. AI virus subtype H5N1 killed chickens within 48-72 hoursdepending on the genetic character of the virus. New AI viruses of subtype H5N1 caused more severe organdamage severe than the character of the old AI viruses. Adaptation of virus in each organ also proved that the viruslikely create variations/quasispecies, which was different from the viral origin.Keywords: Phenotypes, avian influenza, H5N1, genetic character, quasispecies
VIRUS INFLUENZA NOVEL H1N1 BABI DI INDONESIA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3115

Abstract

ABSTRACTNovel H1N1 influenza virus in Swine in Indonesia. Novel H1N1 influenza virus occurred sinceApril 2009 has caused mortality in human population. In Indonesia, this situation requireintensive surveillance to prevent reassortant probability between the H5N1 virus and novelH1N1 virus. This study conduct preliminary surveillance of novel H1N1 virus circulation byusing Real Time-Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR), that validatedby CDC to detect novel H1N1 virus. Result of this study revealed that the influenza novelH1N1 virus was detected in swine/pigs in Indonesia especially in Bulan island and two individualsample from Kapok slaughter house in Jakarta. These findings showed that in Indonesia thenovel H1N1 virus is not only found in human but also has circulated in swine in Indonesia.Key words: pig/swine, influenza novel H1N1 virus
STATUS INFEKSI VIRUS HENDRA PADA KALONG (PTEROPUS SPP.) DI PONTIANAK, KALIMANTAN BARAT DAN MANADO, SULAWESI UTARA Sendow, Indrawati; Field, Hume; Ratnawati, Atik; Adjid, RM. Abdul; Saepulloh, Muharam; Breed, Andrew; Morrissy, Chris.; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i1.144

Abstract

Hendra merupakan salah satu penyakit emerging dan zoonosis yang berbahaya, termasuk Genus Henipavirus(Paramyxoviridae). Penyakit ini sangat erat hubungannya dengan Nipah, yang dapat menginfeksi ternak babi danmanusia. Survey serologi dilakukan di dua propinsi, yaitu Kalimantan Barat dan Sulawesi Utara. Hasil menunjukkanbahwa 148 kalong (Pteropus sp.) yang terdiri dari 84 P. vampyrus asal Kalimanatan Barat dan 64 P alecto asalSulawesi Utara telah dikoleksi. Hasil serologis mengindikasikan 22,6% P vampyrus di Kalimantan Barat mempunyaiantibodi terhadap virus Hendra, yang juga merupakan reaksi silang dengan virus Nipah. Di Sulawesi Utara, 25%serum mengandung antibodi terhadap virus Hendra, dimana 7,8% diantaranya hanya mempunyai antibodi terhadapvirus Hendra. Dari data tersebut dapat disimpulkan bahwa antibodi terhadap virus Hendra terdeteksi padaP. alecto di Sulawesi Utara. Hasil ini merupakan laporan pertama tentang infeksi Hendra pada P. alecto di Indonesia.Adanya perbedaan prevalensi tersebut, dapat disebabkan oleh letak geografis atau spesies kalong yang diuji.Kata kunci: P. vampyrus, P. alecto, antibodi, Hendra, Nipah, Serum Netralisasi
SIRKULASI VIRUS AVIAN INFLUENZA H5N1 TAHUN 2010 : VIRUS GENETIC DRIFT MIRIP A/CK/WEST JAVA/PWT-WIJ/2006 DITEMUKAN DI BEBERAPA KABUPATEN DI SUMATRA DAN JAWA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
Co-Authors Adjid, RM. Abdul Adjid, RM. Abdul Assadah, Nur Sabiq Breed, Andrew Breed, Andrew Daniels, Peter Daniels, Peter Dyah Ayu Hewajuli Field, Hume Field, Hume Indrawati Sendow Morrissy, Chris. Morrissy, Chris. Muharam Saepulloh NLP I Dharmayanti NLP Indi Dharmayanti RISA INDRIANI RISZA HARTAWAN Saepullah, Muharam Sendow, Indrawati