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All Journal JSME MIPA UNIMA Molekul: Jurnal Ilmiah Kimia Fullerene Journal of Chemistry Abdimas : Jurnal Pengabdian Kepada Masyarakat Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Rudi Alexander Repi
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Veterinary

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Journal : Molekul: Jurnal Ilmiah Kimia

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THE CHARACTERISTICS OF BIOACTIVE PEPTIDES AND ANTIBACTERIAL ACTIVITY OF HONEY BEE (Apis nigrocincta) SMITH VENOM, ENDEMIC TO SULAWESI Mokosuli Yermia Semuel; Rudi Alexander Repi
Molekul Vol 10, No 2 (2015)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.861 KB) | DOI: 10.20884/1.jm.2015.10.2.4

Abstract

Apis nigrocincta Smith is a species of honey bee cavity nesting, endemic to Sulawesi. Research that aims to find the composition of the bioactive content of peptides and antibacterial activity of honey bee venom A. nigrocincta Smith has been conducted. Honey bee venom composition was analyzed using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Method and Spectrophotometer UV-Vis Method. Analysis of antibacterial activity, was conducted using a modified agar diffusion method. The results showed that the venom of the honey bee Apis nigrocinctaSmith has five bands of molecules with a molecular weight i.e. 33.54kDa; 21 kDa and 15.43 kDa. The peptide detected were hyaluronidase, fosfolipase A, mellitin, lysofosfolipase or antigen 5. Antibacterial activity was higher than the control ampisilin and antibiotic streptomycin.
Co-Authors Abigael A M Lahia Assa, Evelyn P. A. Debby Jacqualine Jochebed Rayer Eline A. Tuju Emma M Moko Ingkiriwang, Marselino Iren Natalia Simanjuntak Lumapow, Kerol Marthy Taulu Meity Nelltje Tanor Nanlohy, Fanny N. Ray Natalia Antou Timporok, Karlina Verawati I Y Roring Yermia Semuel Mokosuli