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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Life Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Yulita Nurchayati
Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Public Health

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Journal : Biosaintifika: Journal of Biology

 1 
Growth Pattern and Copper Accumulation in Callus of Datura metel Nurchayati, Yulita; Santosa, Santosa; Nugroho, Laurentius Hartanto; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5177

Abstract

This experiment was aimed to evaluate the copper accumulation using callus culture of Datura metel L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl2.2H2O and Na2CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 M. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20th doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl2.2H2O at 5 15 M of level concentration but declined at 20M. The maximum rate of accumulation of Cu (0,1519 mg g-1) was obtained at 15 M. Instead, the addition of Na2CuEDTA at 5 20M of level concentration showed the significant increment while the maximum accumulation was obtained at 20M (0,1420 mg g-1). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of D. metel as that fairly absorbed and accumulated Cu2+. Exposure with CuCl2.2H2O indicated higher accumulation than Na2CuEDTA.How to CiteNurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. Biosaintifika: Journal of Biology & Biology Education, 8(2), 135-140.
The Effect of NAA and Ga3on The Growth Performance of Ceriops tagal Seedling Budihastuti, Rini; Prihastanti, Erma; Nurchayati, Yulita
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13625

Abstract

Plant Growth Regulator (PGR) had been widely utilized in improving plant quality and productivity. The mechanism includes the manipulation of plant adaptibility over environmental stress. This research aimed to study the efect of PGR on the growth of C. tagal seedling and to formulate the applicable treatment to improve its growth performance. The research was conducted through laboratory experiment, including tthe combination of NAA and GA3 with the designed concentration of 0 ppm, 150 ppm and 300 ppm. The experiment lasted for one month and involved weekly observation. The plants were treated by spraying once every three days. Observations were conducted to the survival rate, height increment and leaf number development. ANOVA was conducted as statistical analysis method. The result showed that there were no significant effect of PGRs on the height increment of C. tagal seedling. The survival rate was lowest in the control and 150 ppm NAA treatments, while treatment with 300 ppm GA3was highest. The application of 300 ppm GA3was the best treatment for the livelihood of C. tagal seedling, while the application of 150 ppm GA3 was considerable alternative due to its growth performance. This research provide a novel finding concerning the application of PGRs on the seeding process of C. tagal. Thus, it could be applied in the seeding activities by the mangrove farmers to improve the productivity and quality of mangrove seedling.
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Azura Muzdalifah Istiqomah Diah Nurmaningrum Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Jefri Saputro L. Hartanto Nugroho Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Mochammad Syaifuddin Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Puji Nur Hana Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Santosa Santosa Santosa Santosa Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utami, Ika Nur Wahyu Hadayani