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All Journal Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Life Science
Nintya Setiari
Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health

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Journal : Jurnal Bioteknologi

 1 
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Fella Suffah Meinaswati; Nintya Setiari; Yulita Nurchayati; Sri Widodo Agung Suedy
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.874 KB) | DOI: 10.29122/jbbi.v9i1.5049

Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ. Nepenthes gymnamphora Nees. merupakan spesies endemik Pulau Jawa yang tergolong langka, sehingga perlu upaya konservasi. Konservasi ex situ spesies ini dapat dilakukan dengan teknik kultur jaringan. Penelitian ini bertujuan untuk mengetahui konsentrasi garam mineral media MS dan pepton yang dapat mendukung perkecambahan biji dan menentukan konsentrasi TDZ untuk pertumbuhan kecambah N. gymnamphora in vitro.  Pada percobaan I, biji N. gymnamphora disterilisasi dan ditabur di 4 kombinasi media, yaitu MS, ½MS, dengan dan tanpa penambahan 2g/L pepton. Pada percobaan II, kecambah berukuran ± 0,25 cm dengan penambahan beberapa konsentrasi TDZ (0; 0,5; 1; 1,5 ppm) pada media ½MS. Hasil penelitian menunjukkan bahwa media ½MS menghasilkan persentase perkecambahan biji tertinggi (56%) dengan tinggi kecambah kecambah terbaik. Media ½MS tanpa TDZ menghasilkan pertumbuhan kecambah terbaik yang ditunjukkan oleh waktu tercepat munculnya daun, Media ½MS merupakan konsentrasi garam mineral terbaik untuk perkecambahan biji N. gymnamphora, tanpa TDZ.
Co-Authors Abdul Khafid, Abdul Ade Arisma Fauziah Ade Arisma Fauziah Agustin Noviati Aziz Purwantoro Desi Arisanti Diah Nurmaningrum Endah Dwi Hastuti Endang Saptiningsih Endang Semiarti Erma Prihastanti Ertina Novaria Sitorus Eva Septiyana Fakhri Fadhlurrohman Pratama Farida Nur Hasanah Fauziah, Ade Arisma Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Heny Irawati Ika Indah Selawanti Ika Susanti Hendriyani Jefri Saputro Luaeliyah, Masrukhatul Much. Azam Muhammad Faisol Hakim Muhammad Khusni Hidayat Muhammad Nur Munifatul Izzati NIDA, KHOIRIN Nita Kumalasari Dewi Nurullita Prahasti Prahasti, Nurullita Sri Darmanti Sri Widodo Agung Suedy Sukarti Moeljopawiro Sulistyowati Sulistyowati Surya Kurnia Hayati Tanti Dwi Romawati Ulva, Maria Wahyu Hadayani Yulita Nurchayati