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All Journal Bioma : Berkala Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Bioeksperimen: Jurnal Penelitian Biologi Biosaintifika: Journal of Biology & Biology Education
ENDANG SUTARININGSIH SOETARTO
Laboratorium Mikrobiologi, Fakultas Biologi, Universitas Gadjah Mada, Sekip Utara, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Potential of Soil Bacteria as Mercury Bioremediation Agent in Traditional Gold Mining Winardi, Winardi; Haryono, Eko; Sudrajat, Sudrajat; Soetarto, Endang Sutariningsih
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.284 KB) | DOI: 10.15294/biosaintifika.v11i1.16688

Abstract

Mandor Village has developed as a tradisional gold mining area since years ago. It involved activities that have led to extreme land condition and the release of mining residues, i.e., mercury, to the soils. The study examined the potential of soil bacteria as mercury bioremediation agent based on their population and activity in former mines with different ages. The bacterial population was measured by isolating soil bacteria on solid media using the pour plate method, and the colonies were enumerated during the incubation. The Nutrient Agar (NA) medium was used to obtain the total population, whereas the Salt Base Solution (SBS) was to determine the presence of mercury-tolerant bacteria. The addition of HgCl2 affected the number of the colonies. The colony only grew until the concentration of HgCl2 reached 5 mg/l, and the total colony was larger in older mines. The observation of bacterial activity showed that biotransformation performance was lower when the concentration of mercury was the same as its natural presence in soils (0.1-0.5 mg/l) compared with higher mercury level (1 mg/l). The research showed that lower mercury concentrations in nature reduced the natural ability of bacteria to transform pollutants. This study provides information that can assist the development of a technological approach to control mercury pollution in former traditional gold mines in an environmentally friendly manner using indigenous soil bacteria.
Produk Lipase Kapang Lipolitik pada Limbah Ampas Kelapa Suyanto, Eko; Soetarto, Endang Sutariningsih; Cahyanto, Muhammad Nur
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i1.311

Abstract

Lipase memiliki manfaat penting di bidang industri. Tujuan penelitian ini adalah untuk mendapatkan kapang lipolitik yang mampu tumbuh dan menghasilkan aktivitas lipase tinggi pada limbah ampas kelapa menggunakan metode solid state fermentation. Isolat kapang uji dipurifikasi kemudian dilakukan skrining dan seleksi kapang lipolitik dan dilanjutkan dengan produksi lipase menggunakan substrat ampas kelapa yang sebelumnya diukur kandungan biokimia. Hasil menunjukkan bahwa 8 isolat kapang lipolitik mampu tumbuh baik pada substrat ampas kelapa yang ditunjukkan dengan adanya sporulasi dan perubahan pH medium selama reaksi. Diantara kapang lipolitik tersebut, isolat kapang KLC-333 diketahui menghasilkan aktivitas hidrolisis lipase terbesar yaitu 13,33 U/ml dan volume produksi 46 ml. Biosintesis dan peningkatan produksi lipase dipengaruhi oleh kandungan nutrien di dalam substrat ampas kelapa.
Kinetika Pertumbuhan Dan Produksi Inulinase Fusan F7 Wijanarka, Wijanarka; Soetarto, Endang Sutariningsih; Dewi, Kumala; Indrianto, Ari
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.963 KB) | DOI: 10.14710/bioma.15.2.53-57

Abstract

Pertumbuhan dapat diartikan sebagai suatu pertambahan bagian-bagian sel. Adanya     pertumbuhan sel biasanya dapat diketahui dengan adanya pertambahan ukuran dan     pembelahan sel. Populasi sel khususnya mikroba  secara kuantitatif atau kualitatif dapat digunakan untuk memantau atau mengkaji fenomena pertumbuhan.Enzim inulinase (E.C. 3.2.1.7) adalah enzim yang mampu merombak substrat inulin menjadi monomer fruktosa. Fruktosa merupakan bahan baku (doctoring agent) untuk proses  pembuatan FOS, IOS, pulullan, aseton dan sorbitol.Tujuan  penelitian ini adalah untuk mengetahui kinetika kecepatan pertumbuhan specifik (µ), waktu generasi (g) dan aktivitas inulinase yang dihasilkan oleh fusan F7.  Fusan F7 merupakan hasil fusi antara Pichia manshurica dan Rhodosporidium paludigenum.Hasil penelitian menunjukkan bahwa Fusan F7 mempunyai kecepatan pertumbuhan specific (µ)  sebesar 0.3299 jam dengan waktu generasi (g) 2.1012 jam dan aktivitas enzim inulinase yang dihasilkan sebesar  0.5337 IU. Hasil tersebut  terletak diantara kedua parentalnya yaitu P. manshurica (µ= 0.27935 jam; g = 2.4815 jam dan aktivitas = 0.557 IU) dan Rh. paludigenum (µ= 0.3787 jam; g = 1.8304 jam dan aktivitas = 0.3263 IU). Kata kunci : Pertumbuhan;  fusan F7; inulinase ; umbi dahlia
Kemampuan Fusan F1 Dalam Memproduksi Inulinase Wijanarka, Wijanarka; Soetarto, Endang Sutariningsih; Dewi, Kumala; Indrianto, Ari
Bioma : Berkala Ilmiah Biologi Vol. 16, No.2, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.57 KB) | DOI: 10.14710/bioma.16.2.114-118

Abstract

Fusan F1 was the result of the fusion of the Pichia manshurica and Rhodosporidium paludigenum. The second type of yeast has the ability to produce inulinase.  Inulinase (EC. 3.2.1.7) is an enzyme that is classified as a hydrolase enzyme, this enzyme has the ability to break down complex inulin into simpler components that fructose. Fructose was a monosaccharide with huge potential for the manufacture of butanol, iOS, pullan, FOS and ethanol. The purpose of research to determine the ability fusan F1 in producing inulinase and to determine the specific growth rate (μ), as well as the generation time (g) fusan F1.The results showed that fusan F1 at the 18 thhour was able to produce inulinase of 0.61 mol / min. These results are higher than the parental namely P. manshurica (0.56 mol / min) and Rh. paludigenum (0.33 mol / min). While The specific growth rate (μ) and generation time (g) fusan F1 respecly 0.25 h and 2.7/ h. Keywords: Fusan F1; inulinase; the specific growth; generation time
Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Ali, Alimuddin; Soetarto, Endang Sutariningsih; Widada, J. Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.069 KB) | DOI: 10.24002/biota.v11i2.2625

Abstract

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 mol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.
In Situ Bioremediation Strategies for the Recovery of Mercury-contaminated Land in Abandoned Traditional Gold Mines in Indonesia Winardi, Winardi; Haryono, Eko; Sudrajat, Sudrajat; Soetarto, Endang Sutariningsih
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25229

Abstract

Traditional gold mining activities release mercury into the environment, creating a major concern for the Indonesian governments today. In situ bioremediation, which draws on the activities of indigenous soil bacteria for the recovery of mercury-contaminated land, has never been conducted intensively in the country. This research set out to determine the most efficient in situ bioremediation strategy for this purpose. It took place in Mandor Village, Landak Regency, Kalimantan Barat-Indonesia. During the experiment, four groups of sampling plots were made into triplicate and given various treatments: a. nutrient addition, b. aeration, c. pH neutralization, and d. without nutrient addition and aeration as a control. pH neutralization was conducted in all sampling plots by adding lime until soil pH of ±7 was achieved. The experiment was performed during both rainy and dry seasons to determine the influence of seasonal weather. Total mercury levels of each plot were measured on day 0, 30, 60, 90, and 120, and the effects of treatments and time on mercury depletion were analyzed by two-way ANOVA (P<0.05), followed by a post hoc test to identify the best treatment and optimum time for in situ bioremediation. The results showed that the best time to conduct this bioremediation was in the rainy season by applying nutrient addition and aeration for 90 days on soils with neutral pH; these stimulations could remove ±89.6% of the mercury. This bioremediation technique is a novel technological approach in land recovery that local governments can adopt to restore soils contaminated with mercury from traditional gold mining.  
Co-Authors Ali Djamhuri Alimuddin Ali Ari Indrianto Eko Haryono Eko Suyanto Kumala Dewi Muhammad Nur Cahyanto RETNO PENI SANCAYANINGSIH Rina Sri Kasiamdari Sudrajat Sudrajat Widada, J. Sri Wijanarka Wijanarka Winardi Winardi