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All Journal Bioma : Berkala Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Bioeksperimen: Jurnal Penelitian Biologi Biosaintifika: Journal of Biology & Biology Education
ENDANG SUTARININGSIH SOETARTO
Laboratorium Mikrobiologi, Fakultas Biologi, Universitas Gadjah Mada, Sekip Utara, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Journal : Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

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Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Ali, Alimuddin; Soetarto, Endang Sutariningsih; Widada, J. Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.069 KB) | DOI: 10.24002/biota.v11i2.2625

Abstract

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 mol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.
Co-Authors Ali Djamhuri Alimuddin Ali Ari Indrianto Eko Haryono Eko Suyanto Kumala Dewi Muhammad Nur Cahyanto RETNO PENI SANCAYANINGSIH Rina Sri Kasiamdari Sudrajat Sudrajat Widada, J. Sri Wijanarka Wijanarka Winardi Winardi