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All Journal Jurnal Riset Kimia Makara Journal of Technology
Dyah Styarini
Research Center for Chemistry, Indonesian Institute of Sciences, Puspiptek, Serpong, Tangerang 15340, Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Materials Science & Nanotechnology Mechanical Engineering

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Validation and Uncertainty Evaluation of an LC-DAD Method for Simultaneous Quantification of Benzoic Acid, Methylparaben, and N-Butylparaben in Soy Sauce Aristiawan, Yosi; Ramadhaningtyas, Dillani Putri; Styarini, Dyah
Jurnal Riset Kimia Vol 13, No 1 (2022): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v13i1.432

Abstract

Chemical food preservation is the common strategy used by human to preserve the natural properties and to increase the shelf life of food. Although preservatives are useful to keep the food fresh and to stop the bacterial growth, there are certain preservatives that are harmful if taken in more than the prescribed limits. Some of the typical used—benzoic acid, methylparaben, and n-butylparaben—were employed in this work with the aim of establishing a simultaneous liquid chromatography (LC) method for detecting each in soy sauce matrices. Liquid and C18 solid phase extraction were performed in this procedure prior to LC using Diode Array Detector analysis. In gradient elution of a format buffer (pH 4.4) and acidified acetonitrile, the target components were successfully separated. Calibration curve ranged from 0.61–140 mg/kg linearly while the limit of quantification for benzoic acid, methylparaben, and n-butylparaben were 0.41, 0.10, and 0.11 mg/kg, respectively. The intermediate precision and recovery were in the range between 0.15-1.89% and 100.5-103.3%, respectively. The expanded uncertainty (k=2) in sample measurement was estimated at 3.4-6.5%. The offered method was conformed to the validation acceptance criteria and can be applied as a routine method in the laboratory at ppm level.
Biological Pretreatment of Oil Palm Frond Fiber Using White-Rot Fungi for Enzymatic Saccharification Hermiati, Euis; Anita, Sita Heris; Risanto, Lucky; Styarini, Dyah; Sudiyani, Yanni; Hanafi, Achmad; Abimanyu, Haznan
Makara Journal of Technology Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

Oil palm frond is one type of lignocellulosic biomass abundantly and daily available in Indonesia. It contains cellulose which can be converted to glucose, and further processed to produce different kinds of value –added products. The aim of this research is to study the effects of biological pretreatment of oil palm frond (OPF) fiber using Phanerochaete chrysosporium and Trametes versicolor on the enzymatic saccharification of the biomass. The OPF fiber (40-60 mesh sizes) was inoculated with cultures of the two fungi and incubated at 27 °C for 4 weeks. The samples were taken after 1, 2, 3, and 4 weeks of incubation. Chemical components of the biomass after pretreatment were analyzed. The saccharification of the pretreated samples using cellulase and β-glucosidase was performed in a water bath shaker at 50 °C for 48 hours. The concentration of reducing sugar increased with increasing of incubation time, either in those pretreated with culture of P. chrysosporium or with T. versicolor. Pretreatment of OPF fiber using single culture of T. versicolor for 4 weeks gave the highest reducing sugar yield (12.61% of dry biomass).
Co-Authors Achmad Hanafi, Achmad Euis HERMIATI Haznan Abimanyu Lucky Risanto Ramadhaningtyas, Dillani Putri Sita Anita, Sita Sita Heris Anita Yanni Sudiyani Yosi Aristiawan