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SAKARIFIKASI SERAT TANDAN KOSONG DAN PELEPAH KELAPA SAWIT SETELAH PRETREATMENT MENGGUNAKAN KULTUR CAMPURAN JAMUR PELAPUK PUTIH Phanerochaete chrysosporium dan Trametes versicolor Abimanyu, Haznan; Hanafi, Ahmad; Aristiawan, Yosi; Anita, Sita Heris; Risanto, Lucky; Hermiati, Euis
Jurnal Penelitian Hasil Hutan Vol 32, No 2 (2014): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Litbang Keteknikan Kehutanan dan Pengolahan Hasil Hutan

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Abstract

Penggunaan kultur campuran jamur pelapuk putih pada proses pretreatment bahan lignoselulosa belum banyak diteliti. Penelitian ini bertujuan untuk mempelajari pengaruh penggunaan kultur  Phanerochaete chrysosporium dan Trametes versicolor pada proses pretreatment serat tandan kosong dan pelepah kelapa sawit terhadap hasil sakarifikasinya. Inokulum P. Chrysosporium dan T. versicolor dituangkan ke dalam sampel substrat serat tandan kosong dan pelepah kelapa sawit (40-60 mesh) yang telah disterilkan, masing-masing sebanyak 5% (w/v), sehingga total inokulum yang ditambahkan ke dalam kedua macam substrat masing-masing 10% (w/v). Sampel diinkubasikan pada suhu ±27°C selama 4 minggu. Sebagian dari contoh diambil dan lalu diperiksa setelah masa inkubasi 1, 2, 3, dan 4 minggu. Sakarifikasi dilakukan menggunakan enzim selulase sebanyak 20 FPU per g biomassa dan β-glukosidase dalam shaking waterbath pada suhu 50°C selama 48 jam. Analisis gula pereduksi, glukosa dan xilosa dilakukan terhadap hasil sakarifikasi. Rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari tandan kosong kelapa sawit. diperoleh dari sakarifikasi serat dengan pretreatment selama 4 minggu, yaitu masing-masing 13,08%, 0,86 mg/g dan 0,13 mg/g, sedangkan rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari pelepah kelapa sawit didapatkan dari sakarifikasi substrat dengan pretreatment selama 2 minggu, yaitu masing-masing 8,98%, 0,92 mg/g dan 0,23 mg/g.
Biological Pretreatment of Oil Palm Frond Fiber Using White-Rot Fungi for Enzymatic Saccharification Hermiati, Euis; Anita, Sita; Risanto, Lucky; Styarini, Dyah; Sudiyani, Yanni; Hanafi, Achmad; Abimanyu, Haznan
Makara Journal of Technology Vol 17, No 1 (2013)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.583 KB) | DOI: 10.7454/mst.v17i1.260

Abstract

Oil palm frond is one type of lignocellulosic biomass abundantly and daily available in Indonesia. It contains cellulose which can be converted to glucose, and further processed to produce different kinds of value –added products. The aim of this research is to study the effects of biological pretreatment of oil palm frond (OPF) fiber using Phanerochaete chrysosporium and Trametes versicolor on the enzymatic saccharification of the biomass. The OPF fiber (40-60 mesh sizes) was inoculated with cultures of the two fungi and incubated at 27 °C for 4 weeks. The samples were taken after 1, 2, 3, and 4 weeks of incubation. Chemical components of the biomass after pretreatment were analyzed. The saccharification of the pretreated samples using cellulase and β-glucosidase was performed in a water bath shaker at 50 °C for 48 hours. The concentration of reducing sugar increased with increasing of incubation time, either in those pretreated with culture of P. chrysosporium or with T. versicolor. Pretreatment of OPF fiber using single culture of T. versicolor for 4 weeks gave the highest reducing sugar yield (12.61% of dry biomass).
Experimental Investigation of 2nd Generation Bioethanol Derived from Empty-fruit-bunch (EFB) of Oil-palm on Performance and Exhaust Emission of SI Engine Putrasari, Yanuandri; Abimanyu, Haznan; Praptijanto, Achmad; Nur, Arifin; Irawan, Yan; Simanungkalit, Sabar Pangihutan
Journal of Mechatronics, Electrical Power and Vehicular Technology Vol 5, No 1 (2014)
Publisher : Research Centre for Electrical Power and Mechatronics, Indonesian Istitutes of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/j.mev.2014.v5.9-16

Abstract

The experimental investigation of 2nd generation bioethanol derived from EFB of oil-palm blended with gasoline for 10, 20, 25% by volume and pure gasoline were conducted on performance and exhaust emission tests of SI engine. A four stroke, four cylinders, programmed fuel injection (PGMFI), 16 valves variable valve timing and electronic lift control (VTEC), single overhead camshaft (SOHC), and 1,497 cm3 SI engine (Honda/L15A) was used in this investigation. Engine performance test was carried out for brake torque, power, and fuel consumption. The exhaust emission was analyzed for carbon monoxide (CO) and hydrocarbon (HC). The engine was operated on speed range from1,500 until 4,500 rev/min with 85% throttle opening position. The results showed that the highest brake torque of bioethanol blends achieved by 10% bioethanol content at 3,000 to 4,500 rpm, the brake power was greater than pure gasoline at 3,500 to 4,500 rpm for 10% bioethanol, and bioethanol-gasoline blends of 10 and 20% resulted greater bsfc than pure gasoline at low speed from 1,500 to 3,500 rpm. The trend of CO and HC emissions tended to decrease when the engine speed increased.
Alkaline Pretreatment of Sweet Sorghum Bagasse for Bioethanol Production Sudiyani, Yanni; Triwahyuni, Eka; Muryanto, Muryanto; Burhani, Dian; Waluyo, Joko; Sulaswaty, Anny; Abimanyu, Haznan
International Journal of Renewable Energy Development Vol 5, No 2 (2016): July 2016
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.5.2.113-118

Abstract

Lignocellulosic material, which consist mainly of cellulose, hemicelluloses and lignin, are among the most promising renewable feedstocks for the production of energy and chemicals.   The bagasse residue of sweet sorghum can be utilized as raw material for alternative energy such as bioethanol.  Bioethanol production consists of pretreatment, saccharification, fermentation and purification process.  The pretreatment process was of great importance to ethanol yield.  In the present study, alkaline pretreatment was conducted using a steam explosion reactor at 1300C with concentrations of NaOH  6, and 10% (kg/L) for 10, and 30 min.  For ethanol production separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) process were conducted with 30 FPU of Ctec2 and Htec2 enzyme and yeast of Saccharomyces cerevisiae.   The results showed that maximum cellulose conversion to total glucose plus xylose were showed greatest with NaOH 10% for 30 min.  The highest yield of ethanol is 96.26% and high concentration of ethanol 66.88 g/L were obtained at SSF condition during 48 h process. Using SSF process could increase yields and concentration of ethanol with less energy process. Article History: Received January 16th 2016; Received in revised form May 25th 2016; Accepted June 28th 2016; Available onlineHow to Cite This Article: Sudiyani, Y., Triwahyuni, E., Muryanto, Burhani, D., Waluyo, J. Sulaswaty, A. and Abimanyu, H. (2016) Alkaline Pretreatment of Sweet Sorghum Bagasse for Bioethanol Production. Int. Journal of Renewable Energy Development, 5(2), 113-118.http://dx.doi.org/10.14710/ijred.5.2.113-118 
HYDROTHERMAL LIQUEFACTION OF BLACK LIQUOR INTO PLATFORM CHEMICALS Mansur, Dieni; Simanungkalit, Sabar Pangihutan; Rinaldi, Nino; Abimanyu, Haznan
Teknologi Indonesia Vol 39, No 1 (2016)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.054 KB) | DOI: 10.14203/jti.v39i1.245

Abstract

Currently, much attention is devoted to produce bioethanol based on lignocellulosic materials for alternativefuels. Production of bioethanol from lignocellulosic generates black liquor as a waste during the pretreatmentprocess. The black liquor has a potential to produce platform chemicals. Conversion of black liquor into platformchemicals was carried out by hydrothermal liquefaction by involving water as reaction medium at 200275 C.Through the hydrothermal process combined with water at high temperature, catalytic role could be taken over bysodium sulfate. Increase in temperature up to 275C, activity of water combined with sodium sulfate also increasedin decomposition of block chemicals containing in the black liquor. The black liquor was converted into oxygenatedcompounds, such asphenol, propylene oxide, butyrolactone, and quaiacol. The chemicals were recovered as23, 16, 11 and 8 mol%, respectively.
Catalytic Performance of Sulfonated Carbon Catalysts for Hydrolysis of Palm Oil Empty Fruit Bunch Kristiani, Anis; Sembiring, Kiky Corneliasari; Aristiawan, Yosi; Aulia, Fauzan; Hidayati, Luthfiana Nurul; Abimanyu, Haznan
Jurnal Kimia Sains dan Aplikasi Vol 23, No 6 (2020): Volume 23 Issue 6 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3393.171 KB) | DOI: 10.14710/jksa.23.6.209-215

Abstract

Utilizing lignocellulosic biomass into valuable products, such as chemicals and fuels, has attracted global interest. One of lignocellulosic biomass, palm oil empty fruit bunch (EFB), has major content of cellulose (30-40%), which is highly potential to be a raw material for fermentable sugar production. In this research, a series of sulfonated carbon catalysts with various concentrations of sulfuric acid (H2SO4, 10-30 v/v%) solutions have been successfully prepared and applied for a single stage of heterogeneous acid-catalyzed hydrolysis over microcrystalline cellulose and EFB under moderate temperature condition and ambient pressure. The catalysts’ physical and chemical properties were characterized by using a Thermogravimetric Analyzer (TGA), X-ray diffractometer, surface area analyzer, and Fourier-transform infrared spectrophotometer. The characterization results showed that sulfonated carbon had relatively similar physical properties with the parent of active carbon. The hydrolysis activity of sulfonated carbon catalysts gave various Total Reducing Sugar (TRS). The effects of sulfate loading amount in catalyst samples and various ionic liquids were investigated. The hydrolysis of pure microcrystalline cellulose powder (Avicel) using 30%-sulfonated carbon (30-SC) catalyst in 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) ionic liquid at 150°C yielded the highest TRS of 16.11%. Subsequently, the catalyst of 30-SC was also tested for hydrolysis of EFB and produced the highest TRS of 40.76% in [BMIM]Cl ionic liquid at 150°C for 4 h. The obtained results highlight the potential of sulfonated carbon catalysts for hydrolysis of EFB into fermentable sugar as an intermediate product for ethanol production.
Biological Pretreatment of Oil Palm Frond Fiber Using White-Rot Fungi for Enzymatic Saccharification Hermiati, Euis; Anita, Sita Heris; Risanto, Lucky; Styarini, Dyah; Sudiyani, Yanni; Hanafi, Achmad; Abimanyu, Haznan
Makara Journal of Technology Vol. 17, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Oil palm frond is one type of lignocellulosic biomass abundantly and daily available in Indonesia. It contains cellulose which can be converted to glucose, and further processed to produce different kinds of value –added products. The aim of this research is to study the effects of biological pretreatment of oil palm frond (OPF) fiber using Phanerochaete chrysosporium and Trametes versicolor on the enzymatic saccharification of the biomass. The OPF fiber (40-60 mesh sizes) was inoculated with cultures of the two fungi and incubated at 27 °C for 4 weeks. The samples were taken after 1, 2, 3, and 4 weeks of incubation. Chemical components of the biomass after pretreatment were analyzed. The saccharification of the pretreated samples using cellulase and β-glucosidase was performed in a water bath shaker at 50 °C for 48 hours. The concentration of reducing sugar increased with increasing of incubation time, either in those pretreated with culture of P. chrysosporium or with T. versicolor. Pretreatment of OPF fiber using single culture of T. versicolor for 4 weeks gave the highest reducing sugar yield (12.61% of dry biomass).