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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Majalah Kedokteran Bandung Juke Jurnal Agroindustri Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Teknologi & Industri Hasil Pertanian JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Agrotek Tropika Jurnal Teknologi Pertanian Andalas JK Unila (Jurnal Kedokteran Universitas Lampung) Jurnal Inovasi Pembangunan Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Jurnal Pengembangan Agroindustri Terapan

Subeki Subeki

Jurusan Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Lampung

Author-ID : 387104

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Transportation Other

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POTENCY OF THE INDONESIAN MEDICINAL PLANTS AS ANTIMALARIAL DRUGS Subeki, Subeki
Jurnal Teknologi & Industri Hasil Pertanian Vol 13, No 1 (2008): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

The Indonesian traditional herbal medicine has been practiced for many centuries in Indonesia to treat malaria diseases. Although modern medicine is becoming increasingly important, herbal medicine is still very popular. In order to select raw material for preparation of safety herbal medicines, forty five medicinal plants have been tested for acute toxicity in mouse at a dose 715 mg/kg body weight. The extracts of Asclepias curassavica leave, Alstonia scholaris leave, Decospermum fruticosum leave, Elaocarpus petiolatus bark, Elaocarpus parvifolius bark, Eurycoma longifolia root, Garcinia rigida bark, Nephelium lappaceum bark, Pentaspodan motleyi leave, Picrasma javanica leave, Phyllanthus niruri whole, Quassia indica leave, Syzygium pycnanthum bark, Tetrasera scandens leave, Cratoxylum glaucum bark, Sandoricum emarginatum bark, Mallotus paniculatus leave, Microcos ovatolanceolata bark, Poikilospermum suaveolens leave, Fibraurea chloroleuea leave, Tetrasera scandens root, and Timonius billitonensis bark showed toxicity with mortality level of 20-100%. The remaining 32 plant extracts were not toxic at dose tested. The toxic plant species should be considered in the preparation of herbal medicines. Of the safety extracts were tested for their antimalarial activity against Plasmodium berghei in vivo at a dose 715 mg/kg body weight. Extract of Carica papaya leave was most active than other plant extracts with parasitemia 1.13%, while control showed 17.21%. More research is needed to scientifically prove efficacy and to identity antimalarial constituents in the plant extracts. Key words: Indonesian medicinal plant, jamu, toxicity, antimalarial activity, Plasmodium berghei.

KAJIAN TOKSISITAS EKSTRAK DAUN MINT (Mentha arvensis L.) TERHADAP MORTALITAS ULAT KROP KUBIS (Crocidolomia pavonana F.) Ekaristi, Teresia Clara; Yasin, Nur; Hariri, Agus Muhammad; Subeki, Subeki
Jurnal Agrotek Tropika Vol 2, No 1 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Ulat krop kubis dapat merusak tanaman dengan cara memakan bagian titik tumbuh kubis. Untuk mengendalikannya dapatdigunakan insektisida botani. Salah satu diantaranyaadalah dengan mengkaji ekstrak daun mint (Mentha arvensis L.). Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun mint terhadap Crocidolomia pavonana F. Penelitian ini dilaksanakan di Laboratorium Komponen Bioaktif Jurusan Teknologi Hasil Pertanian dan Laboratorium Bioteknologi Pertanian, Fakultas Pertanian Universitas Lampung pada bulan Januari sampai Oktober 2012. Penelitian dilakukan melalui 3 tahap uji hayati. Uji hayati pertama dilakukan untuk mengetahui aktivitas insektisida antara fraksi lapisan air (H2O) dan etil asetat (Et OAc) tehadap mortalitas C. pavonana F. Fraksi yang aktif adalah fraksi lapisan air. Fraksi ini diuapkan dan dielusi menjadi 6 fraksi yaitu 100% H2O, 20% MeOH/H2O, 40% MeOH/H2O, 60% MeOH/H2O, 80% MeOH/H2O, dan 100% MeOH untuk uji hayati kedua. Fraksi 100% H2O menunjukkan aktivitas insektisida lebih tinggi dibandingkan dengan fraksi lainnya. Fraksi 100% H2O dievaporasi hingga diperoleh residu dan dibuat konsentrasi 10.000, 5.000, 2.500, 1.250, 625, dan 312,5 ppm, untuk uji hayati ketiga. Dari uji ini diketahui bahwa aplikasi ekstrak daun mint pada konsentrasi 10.000 ppm mulai 48 jsa dapat menyebabkan mortalitas ulat C. pavonana F. lebih tinggi dibandingkan dengan konsentrasi 5.000, 2.500, 1.250, 625, dan 312,5 ppm.

SENYAWA BRUSEIN-A DARI BUAH MAKASAR (BRUCEA JAVANICA (L.) MERR.) SEBAGAI ANTIPROLIFERASI TERHADAP SEL KANKER PAYUDARA T47D Subeki, -; Muhartono, -
Majalah Kedokteran Bandung Vol 47, No 1 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.059 KB)

Pada saat ini,insidensi kanker payudara telah menempati urutan teratas pada wanita penderita kanker di negara berkembang termasuk Indonesia, sehingga pencarian obat yang lebih tepat masih diperlukan. Tujuan penelitian ini untuk menentukan aktivitas antiproliferasi senyawa brusein-A terhadap sel kanker payudara T47D secara in vitro. Brusein-A diisolasi dari buah makasar (Brucea javanica (L.) Merr.) dan struktur senyawa yang diperoleh dielusi dengan spektroskopi data. Aktivitas antikanker diamati sebagai antiproliferasi terhadap sel kanker payudara T47D. Antiproliferasi diuji dengan penambahan sulfo rodamin B pada media kultur sel. Masing-masing sumur mengandung 30.000 sel yang diberikan brusein-A dengan variasi 10 konsentrasi antara lain 0,04 µg/mL, 0,08 µg/mL, 0,16 µg/mL, 0,31 µg/mL, 0,63 µg/mL, 1,25 µg/mL, 2,5 µg/mL, 5 µg/mL, 10 µg/mL, dan 20 µg/mL selama 72 jam. Percobaan diulang dua kali dan selanjutnya persentase viabilitas sel dihitung. Hasil penelitian menunjukkan bahwa nilai IC50 brusein-A terhadap sel T47D memberikan efek antiproliferasi sebesar 0,54 µg/mL dengan cisplatin 0,43 µg/mL sebagai kontrol positif. Simpulan, brusein-A mempunyai aktivitas antiproliferasi terhadap sel kanker payudara T47D. [MKB. 2015;47(1):22?8]Kata kunci: Antiproliferasi, buah makasar, Brucea javanica, brusein-A, sel kanker T47DBrucein-A Compound of Makasar Fruit (Brucea javanica (L.) Merr.) as Antiproliferative Agent against T47D Breast Cancer CellsBreast cancer is currenly the number one cancer of all cancers attacking women, especially those who live in developing countries including Indonesia. Therefore, the pursuit of drugs for breast cancer is still needed. The aim of this study was to determine the anti proliferative activity of brucein-A against breast cancer T47D cells in vitro. Bruceine-A was isolated from ?buah makasar? (Brucea javanica (L.) Merr) and the structure of the isolated compound was elucidated by spectral data. The activities of anticancer were evaluated from the antiproliferative effects on T47D breast cancer cell line . The antiproliferative effects were examined in cultured cells stained with sulforhodamin B. Each well contained 30,000 cells, which was treated with bruceine-A in 10 concentration variations of 0.04 µg/mL, 0.08 µg/mL, 0.16 µg/mL, 0.31 µg/mL, 0.63 µg/mL, 1.25 µg/mL, 2.5 µg/mL, 5 µg/mL, 10 µg/mL, and 20 µg/mL for 72 hours. The experiment was replicated twice and the inhibitory percentage of cells was then calculated. The results showed that the IC50 value of bruceine-A on T47D cells as anantiproliferative effect was0.54 µg/mL with cisplatin 0.43 µg/mL as the positive control. In conclusion, bruceine-A has an antiproliferative activity on T47D breast cancer cell line. [MKB. 2015;47(1):22?8]Key words: Antiproliferative, buah makasar, Brucea javanica, brucein-A, cancer T47D cell DOI: 10.15395/mkb.v47n1.394

Bioactive Compounds Isolated from Lignin of Empty Bunch Palm Fiber and Their Effects on in Vitro Rumen Fermentation M. Prayuwidayati; T. C. Sunarti; . Sumardi; . Subeki; K. G. Wiryawan
Media Peternakan Vol. 38 No. 3 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.513 KB)

The objective of this experiment was to study the effects of bioactive compounds isolated from purified lignin formacell (PLF) of empty bunch palm fiber as natural antimicrobes and their effects on in vitro rumen fermentation. The first experiment was inhibition test of 11 bioactive compounds isolated from PLF, using disc diffusion method against the growth of Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus. Four of the most potential bioactive compounds were then used in the second experiment, which was an in vitro test using fresh rumen liquid of Ongole grade beef cattle, to study their effects on rumen fermentation. Six treatments with 3 replications were applied in a completely randomized block (CRB) design. The treatments were R0= 0.5 g basal diet; R1= R0 + 0.3 mg Rumensin®; R2= R0 + 0.3 mg syringaldehyde; R3= R0 + 0.3 mg p-hydroxybenzoic acid; R4= R0 + 0.3 mg m-hydroxybenzoic acid; and R5= R0 + 0.3 mg oxybenzene. Isolate fraction of CC-2 (syringaldehyde), CC-3 (m-hydroxybenzoic acid), VLC-5 (oxybenzene), and VLC-9 (p-hydroxybenzoic acid) exhibited antimicrobes activity against all tested bacteria. Other isolated fractions exhibited antimicrobes activity only against 1 or 2 tested bacteria. The use of syringaldehyde, p-hydroxybenzoic acid, m-hydroxybenzoic acid, and oxybenzene improved ammonia concentration, microbial protein synthesis, and nutrients digestibility. Bioactive compounds had no effect on rumen pH but reduced total VFA concentration as well as the estimate of methane production.Key words: bioactive compounds, natural antimicrobes, rumen fermentation

Pengaruh Pemberian Infusa Kopi dalam Menurunkan Kadar Glukosa Darah Mencit yang Diinduksi Aloksan - Subeki; - Muhartono
JUKE Unila Vol 5, No 9 (2015)
Publisher : Fakultas Kedokteran Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.763 KB)

Diabetes adalah penyakit yang ditandai dengan kadar glukosa yang tinggi pada darah. Penyakit ini menjadi masalah kesehatan di Indonesia. Penanganan penyakit diabetes dilakukan dengan pemberian insulin atau obat antidiabetes. Namun demikian, pemberian obat ini sering menimbulkan efek samping bagi penderita diabetes. Oleh karena itu, perlu dicari obat antidiabetes yang aman dan dapat menurunkan kadar glukosa darah. Salah satu alternatif adalah dengan menggunakan infusa kopi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa kopi terhadap kadar glukosa darah mencit yang diinduksi aloksan. Penelitian ini menggunakan 27 ekor mencit yang dibagi menjadi 9 kelompok. Satu kelompok mencit digunakan sebagai kontrol normal dan kelompok mencit lainnya diinduksi aloksan 4,8 mg/g bb dan diberikan infusa kopi dosis 0, 6, 12, 18, 24, 30, 36, dan 42 mg/kg bb sehari sekali selama 7 hari mulai hari ke-8 pasca induksi aloksan. Pengukuran kadar glukosa darah dilakukan pada hari ke-7 dan ke-28 setelah induksi aloksan. Hasil penelitian menunjukkan bahwa infusa kopi dosis 36 mg/kg bb dapat menurunkan kadar glukosa darah menjadi normal dengan kadar glukosa darah sebesar 154 mg/dL. Simpulan, infusa kopi dapat menurukan kadar glukosa darah. [JuKe Unila 2015; 5(9):1-8]

STUDY ON ASCORBIC ACID FOR FORTIFICATIONOF RICE CASSAVA Harun Al-Rasyid; Subeki Subeki; Wisnu Satyajaya; Agus Saptomi
Jurnal Agroindustri Vol 7, No 2 (2017)
Publisher : BPFP Faperta UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agroindustri.7.2.72-83

Siger rice is an analog rice made from agricultural materials containing carbohydrates such as cassava. The purpose of this research was to know the effect of addition of ascorbic acid and steam duration to the quality of siger rice from cassava. The factorial experiment arranged in a Complete Randomized Block Design (CBRD) with two factors and three replications.. The first factor was the addition of ascorbic acid is 0% (A1), 0.1% (A2), 0.15% (A3), 0.2% (A4), 0.25% (A5), and 0.3% A6). The second factor was steam duration of 25 minutes (L1), 30 minutes (L2), and 35 minutes (L3). The data were analyzed using analysis of variance (ANNOVA) and continued with Least Significance Different (LSD) test. The results showed that the addition of ascorbic acid 0.2% with steaming for 35 minutes resulted in the best quality of siger rice with white color tending, somewhat similar to rice, rather soft, water content of 10.62%, 0.88% ash, protein 3,82%, fat 2.42%, crude fiber 1.13%, carbohydrates 81.12%, and vitamin C 0.61 mg/g.

Survei Cemaran Mikroba dan Mutu Ikan Tongkol (Euthynnus Affinis) Segar Dewi Sartika; Anitsa Asrining Puri; Subeki User
Inovasi Pembangunan : Jurnal Kelitbangan Vol 4 No 02 (2016): August 2016
Publisher : Balitbangda Provinsi Lampung

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According to Lu et al. (2010), one of the fishing products that has high in nutritionis is swordfish. Swordfish poisoning that often occurs is histamine poisoning (scombroid fish poisoning). This is caused by pathogen bacterial contamination such as Escherichia coli, Salmonella, Vibrio cholerae, Enterobacteriacea, etc (Kurniawan et al, 2012). The aim ofthis researchwas to know the difference of microbes contamination and physical appearance on the swordfish (Euthynnus affinis) from traditional markets, modern and fish auction markets in Bandar Lampung.This research was done in two stages. The first stage was done collecting swordfish samples randomlyfrom traditional, modern, and auction markets in Bandar Lampung. There were 13 traditional markets, 7 modern markets, and 2 warehouse markets in Bandar Lampung. The second stage of research were do total analysis of microbes, the total plate count (TPC) test, Eschericia colitest, Salmonella sp.test, and freshness level on swordfish using organoleptic test. Then the data of observed total colony and the results data of the organoleptic test were analyzed using BNJ test. The conclusion of this research were be found a difference and swordfishfreshness level on traditional, modern, and auction markets in Bandar Lampung.The lowest value of total plate count 6,5 x 104, Eschericia coli1,53 x 104, and Salmonella sp3 x 101.

Pengaruh Jenis Mikroorganisme dan Lama Fermentasi Terhadap Protein Residu Produk Fermentasi Hasil Samping Udang Yudha Aditya Mahendra; Harun Al Rasyid; Subeki User
Inovasi Pembangunan : Jurnal Kelitbangan Vol 4 No 02 (2016): August 2016
Publisher : Balitbangda Provinsi Lampung

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Shrimp byproduct is theprocessing shrimp result that still can be used by improving its content through the fermentation process using microorganism. The purpose of this research is to determine the influence of microorganism types and fermentation time using to protein residue of shrimp byproduct fermentation. The research was arranged in factorials in the design random and completed with 2 factors and 3 repetitions.The first factor is types of microorganism, that are Aspergillus niger (M1), Rhizopus oryzae (M2), and Lasiodiplodia theobromae (M3). The second factor is the times of fermentation, that are 0 hours (L0), 24 hours (L1), 48 hours (L2), and 72 hours (L3). Similarities of the data were analyzed and analyzed further by using comparison test and orthogonal polynomials at the level 1% and 5%. The result of research shows that microorganism types and fermentation time have very real impact on protein residue and total yield. Each mold has a tendency in quadratic on total yield and protein residue. But R. oryzae did not have real impact on total yield. The best value of protein residue is A. niger at 4,00% with 35,63 hours of fermentation and the best value of total yield is L. theobromae at 65,92% with 27,70 hours of fermentation.

PENGARUH JUMLAH PELARUT ETANOL DAN SUHU FRAKSINASI TERHADAP KARAKTERISTIK LEMAK KAKAO HASIL EKSTRAKSI NON ALKALIZED COCOA POWDER [The effect of amount of ethanol solvent and temperature fractionation on the characteristic of cocoa fat from the extracti Carolina Maria Susanti; Ribut Sugiharto; Sri Setyani; Subeki Subeki
Jurnal Teknologi & Industri Hasil Pertanian Vol 19, No 3 (2014): Jurnal Teknologi Industri & Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

Cocoa and its derivative products are examples of very potential export comdities that could contributed to an increase in foreign exchange. The extraction of non-alkalized cocoa powder resulted in pure cocoa extract as the main product and cocoa fat as by product. Cocoa fat can be exploited further to produce some final products, either food or non food products. This research was aimed to study the effects of ethanol addition for extraction and fractionation temperature on the characteristic of cocoa fat. This study used a Complete Randomized Block Design with two factor treatments and three replications. The first factor was quantity of ethanol 450 ml, 600 ml, and 750 ml, and the second factor was temperature for fractionation 15oC, 18oC, and 21oC. The data obtained were analyzed using Bartlett test for homogenity. Tuckey Test was used for analyzing their additivity, then the data were analyzed using ANOVA to see differences among treatments. The data were further tested using Least Significant Difference at of 5% level of significancy to find the best characteristic. The results showed that the best characteristic of cocoa fat obtained from the treatment of the addition of 750 ml ethanol and fractionation temperature 15oC. It resulted in fat yield of 35.0382%, fat content of 86.742%, its flavor was close to characteristic of cocoa flavor ,and the composition of unsaturated fatty acids was 49, 71% of the total fatty acids. Keywords: cocoa fat, ethanol solvent, fractionation temperature

EVALUASI NILAI CACAT DAN CITA RASA KOPI ROBUSTA (Coffea canephora L.) YANG DIPRODUKSI IKM KOPI DI KABUPATEN TANGGAMUS [Evaluation of Defect Value and Flavour of Robusta Coffee (Coffea canephora L.) Produced by Small and Medium Industry Sector of Coffee in Tanggamus District] Sri Setyani; Subeki Subeki; Henrica Agustina Grace
Jurnal Teknologi & Industri Hasil Pertanian Vol 23, No 2 (2018): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

The purpose of this study was to evaluate the defects, moisture content, flavor, and overall acceptance of Robusta coffee from 20 coffee producers in Tanggamus Regency. Taste testing involved some expert panelists. The data were analyzed descriptively. The results showed that as much as 70% of coffee quality was following SNI 01-2907-2008. The defect value of coffee was at the quality level of 5 until the inferior original quality (“kopi asalan”). The main percentages of the defects were broken seeds, brown beans, black seeds and then followed by hollow seeds. The water content of the coffee was varied between 5.07-7.27. The taste of coffee was dominated by green/grassy, the average taste was slightly bitter, the colors ranged from brown, black and cinnamon-like color, while, the overall acceptance was somewhat like. This results indicated that standard operating procedure is required to be implemented by coffee producers (IKM) in Tanggamus Regency to improve the quality of the coffee.

Co-Authors - Muhartono - Muhartono . Sumardi Agus Muhamad Hariri Agus Muhammad Hariri Agus Muhammad Hariri, Agus Muhammad Agus Saptomi Amelia Hestiana Anitsa Asrining Puri Carolina Maria Susanti Dewi Sartika Dewi Sartika Eka Yulianita Ekaristi, Teresia Clara Erdi Suroso Gusri Ahyar Ibrahim Gusri Ahyar Ibrahim Hamim Sudarsono Harun Al Rasyid Harun Al Rasyid Harun Al-Rasyid Henrica Agustina Grace Indra Pratama Putra Azis Irwan Sukri Banuwa K. G. Wiryawan M. Perdiansyah Mulia Harahap M. Prayuwidayati Maria Erna Kustyawati Muhammad Ferdiansyah Mulya Harahap Muhartono Muhartono Nur Yasin Nur Yasin Nur Yasin Okta Sekar Ningtyas Ribut Sugiharto Rully Pebriansyah Septianita Eva Rozani Sri Hidayati Sri Setyani Sri Setyani suci hardina rahmawati Susilawati Susilawati T. C. Sunarti Tanto Pratondo Utomo Tanto Pratondo Utomo Wisnu Satyajaya Yudha Aditya Mahendra

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