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All Journal Jurnal Veteriner Reaktor Jurnal Agripet Animal Agricultural Journal Jurnal Ilmu dan Teknologi Peternakan Tropis Caraka Tani: Journal of Sustainable Agriculture JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Aplikasi Teknologi Pangan Jurnal Litbang Provinsi Jawa Tengah Livestock and Animal Research
Bambang Sulistiyanto
Departement Of Animal Science, Faculty Of Animal And Agricultural Sciences, Diponegoro University Jl. Prof. Soedarto, SH, Tembalang, Semarang 50275
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Materials Science & Nanotechnology Public Health Social Sciences Veterinary Other

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KUALITAS SERAT LIMBAH PERTANIAN DAN HASIL SAMPING PERTANIAN YANG DIFERMENTASI DENGAN Aspergilus Niger PADA ARAS DAN LAMA PEMERAMAN YANG BERBEDA Sukarti, Enik; Sulistiyanto, Bambang; Mukodiningsih, Sri
Animal Agriculture Journal Vol 1, No 2 (2012): Volume 1, Nomor 2, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

Penelitian untuk mengkaji pengaruh fermentasi limbah Pertanian dan hasil samping pertanian dengan A. niger pada berbagai aras dan lama pemeraman yang berbeda terhadap komponen serat kaitannya dengan pemanfaatan limbah lokal telah dilaksanakan Januari-Maret 2012 di Laboratorium Teknologi Makanan Ternak Jurusan Nutrisi dan Makanan Ternak Fakultas Peternakan Universitas Diponegoro Semarang. Materi penelitian adalah jerami jagung, jerami padi, dedak padi, onggok, ampas brem, ampas tahu, A. niger. Bahan kimia yang digunakan antara lain larutan deterjen netral, larutan deterjen asam, Natriumsulfit (Na2SO3), aseton, asam asetat dan air panas. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap pola faktorial 4x4 masing-masing terdiri dari 3 kali. Parameter yang diukur adalah komponen serat acid detergent fiber (ADF) dan neutral detergent fiber (NDF) . Pengolahan data menggunakan analisis ragam untuk mengetahui pengaruh perlakuan dan jika terdapat pengaruh nyata dilanjutkan dengan uji wilayah ganda Duncan untuk mengetahui perbedaan dan pengaruh perlakuan pada taraf 5%. Hasil penelitian menunjukkan bahwa tidak ada interaksi pengaruh peningkatan aras starter dan lama waktu pemeraman terhadap kandungan NDF dan ADF. Namun, secara parsial berpengaruh nyata (p<0,05) terhadap penurunan kandungan NDF dan ADF. Disimpulkan bahwa perlakuan peningkatan aras starter hingga 6% dan lama pemeraman hingga 21 hari secara parsial menurunkan kadar ADF dan NDF. Penambahan Aras A. niger sampai 4% dan lama pemeraman selama 14 hari masing-masing menghasilkan kadar NDF dan ADF terendah.Kata kunci : limbah pertanian dan hasil samping pertanian, fermentasi, A. niger, komponen seratABSTRACTResearch to assess the effect of fermentation waste Agriculture and agricultural byproducts by A. niger on various old cedars and different curing the fiber components related to the use of local waste has been carried out from January to March 2012 in the Department of Laboratory Animal Food Technology and Nutrition Faculty of Animal Science, Diponegoro University Semarang. Material research is corn straw, rice straw, rice bran, cassava, pulp brem, tofu, A. niger. Chemicals used include neutral detergent, acid detergent, Natriumsulfit (Na2SO3), acetone, acetic acid and hot water. Experimental design used was completely randomized factorial design 4x4 each consisting of 3 times. Parameters measured were acid detergent fiber component fiber (ADF) and neutral detergent fiber (NDF). Data processing using various analysis to determine the effect of treatment and if there is a real effect followed by Duncan multiple test area to determine the differences and the effect of treatment on the level of 5%. The results showed that there was no interaction effect of elevated cedar and long curing time starter for the content of NDF and ADF. However, partially significant (p <0.05) to decrease the content of NDF and ADF. It was concluded that the treatment increased the cedar starter up to 6% and up to 21 days curing time partially reduce levels of ADF and NDF. The addition of A. niger Aras to 4% and the long curing for 14 days each produce the lowest levels of NDF and ADF.Keywords: agricultural waste and agricultural byproducts, fermentation, A. niger, fiber components
KANDUNGAN BAKTERI ASAM LAKTAT DAN BAKTERI SELULOLITIK PADA POLLARD YANG DIFERMENTASI (Content Of Lactid Acid Bacteria And Cellulolytic Bacteria On The Pollard Fermentation) Nurhalimah, Neneng; Widiyanto, Widiyanto; Sulistiyanto, Bambang
Animal Agriculture Journal Vol 4, No 1 (2015): Volume 4 Nomor 1 Tahun 2015
Publisher : Animal Agriculture Journal

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Abstract

ABSTRAK Penelitian untuk mengetahui kandungan bakteri asam laktat (BAL) dan bakteri selulolitik pada pollard yang difermentasi dengan cairan rumen dan Cairan Limbah Sayur Fermentasi (CLSF).  Pembuatan CLSF dengan mencampur limbah sayuran kubis 80% dan sawi 20%, serta garam 8% dan molases 6,4% dari total limbah sayur, kemudian diperam selama 6 hari dan diperas untuk diambil ekstraknya.  Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4 dengan 4 ulangan.  Faktor pertama adalah pemeraman 0 dan 48 jam dan faktor kedua adalah rasio ELSF:cairan rumen 0:0 (B0); 20:10 (B1); 20:20 (B2) dan 10:20 (B3).  Analisis bakteri asam laktat dan bakteri selulolitik menggunakan metode hitungan cawan tuang (Standard Plate Count).Data dianalisis menggunakan analisis ragam.Hasil penelitian menunjukkan bahwa pengaruh perlakuan pemeraman sangat nyata (p<0,05) terhadap kandungan BAL. Fermentasi ELSF:cairan rumen pada pemeraman 48 jam dan perlakuan B3 terbukti mampu mempengaruhi jumlah kandungan BALyaitu sebesar 4,05 x 107sel/g, jumlah ini lebih besar dibandingkan pada saat pemeraman 0 jam dengan perlakuan yang sama yaitu sebesar 0,075x 107sel/g.  Simpulan penelitian ini adalah lama waktu fermentasi dapat meningkatkan jumlah BAL dan jumlah nutrisi yang masih memungkinkan untuk berlangsungnya metabolisme BAL, namun pada perlakuan dan pemeraman yang sama bakteri selulolitik tidak mampu untuk tumbuh.  Perlukajian lanjut terhadap kemungkinan penambahan rasio CLSF dan cairan rumen serta lama waktu fermentasi agar bakteri selulolitik mampu berproliferasi. Kata Kunci:starter, CLSF, cairan rumen, bakteri asam laktat, bakteri selulolitik. ABSTRACT           Experimentto determine the content of lactic acid bacteria (LAB) and cellulolytic bacteria tothe pollard fermentation with rumen fluid and extractoffermentedvegetablewastes(EFVW).  Making the EFVW, mixing the wastes of cabbage 80% and mustard 20% with salt 8% and molasses 6.4% of the total vegetable wastes, then cured for 6 days, and it was mechanically exctrcated to get the EFSW.  This research using a complety randomized design (CRD) factorial (2x4) with 4 replication.  The first factor are incubation times, 0 and 48 hours, second factors ratio of EFVW:rumen fluid  (B0); 20:10 (B1); 20:20 (B2) and 10:20 (B3).  Analyzes of lactid acid bacteria (LAB) and cellulolytic bacteria (CB)were conducted by themethod of standard plate count.  Data were analyzed  of analyst  variant.  The result showed that there was interaction of treatment is significant (p<0,05) on the content of LAB.  Fermentation of EFVW:rumen fluid in 48 hours incubationof B3-treatment afford affect the content with the number 4.05 x 107cell/g the number is greater than 0 hours with time the same is 7.5 x 105.  This result indicate that a timeof fermentation can increase the number of bacteria and the nutrient still allows of the LAB-metabolism, however treatments of ratio EFVW:RF as well as time of curing made the cellulolytic bacteria have not proliferate. Keywords: starter, EFVW, rumen fluid, lactis acid bacteria, cellulolityc bacteria
KUALITAS CHIP BERBAHAN DASAR ONGGOK DAN EKSTRAK LIMBAH SAYUR FERMENTASI DILIHAT DARI BAKTERI ASAM LAKTAT DAN BAKTERI GRAM Mahfudi, Sholikhul; Sulistiyanto, Bambang
Animal Agriculture Journal Vol 1, No 2 (2012): Volume 1, Nomor 2, Tahun 2012
Publisher : Animal Agriculture Journal

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Abstract

Onggok adalah bahan pakan lokal umum yang memiliki potensi sebagai sumber karbohidrat untuk pakan unggas. Onggok dapat ditingkatkan menjadi pakan fungsional bahkan jika diperkaya dengan bakteri fungsional seperti bakteri asam laktat (BAL). Penelitian ini bertujuan untuk mempelajari kualitas mikrobiologi chip onggok yang terbuat dari onggok yang ditambahkan dengan ekstrak limbah sayur fermentasi (ELSF) dengan melihat kandungan bakteri asam laktat dan bakteri Gram. Bahan yang digunakan dalam penelitian ini adalah onggok , ELSF yang dibuat dengan metode Sulistiyanto dan Nugroho. Percobaan ini dilakukan dengan disain acak lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Perlakuan yang diterapkan adalah tingkat perbedaan ELSF dengan cara 0% (T0), 40% (T1), 60% (T2)%. Hasil penelitian menunjukkan bahwa penambahan ELSF dalam membuat chip secara signifikan mempengaruhi jumlah total bakteri dalam chip (P <0,05), namun, tidak ada efek signifikan diberikan terhadap keberadaan bakteri gram. Di antara perlakuan (TO, T1 dan T2), yang masing-masing kandungan BAL 1,2 X102, 1,0 x103 dan 2,3 x104. Menganggap ke skor kehadiran Gram + / - bakteri, antar perlakuan masing-masing 1,00; 1,70 dan 1,90. Hal ini dianggap bahwa produk chip mengandung cukup bakteri gram postive yaitu BAL, tetapi bakteri gram negatif masih ditemukan.Kata kunci: onggok, ELSF, BAL, bakteri Gram ABSTRACT Cassava meal (onggok) is a common local feedstuff that has potential as a source of carbohydrates for the poultry feed. It can be improved to be functional feed even if enriched with functional bacteria such as lactic acid bacteria (LABs). This study aimed to study microbiological quality of cassava chips made from cassava meal that added with the extract fermented vegetable wastes (EFVW) by obeserving the presence of LABs and Gram bacteria. The material used in this study is cassava meal (onggok), EFVW that made by method of Sulistiyanto and Nugroho. The Experiment was conducted by completely randomized design (CRD) with 3 treatments and 5 replications. The treatment applied is the difference level of ELSF by means 0% (T0), 40% (T1), 60% (T2)%. The results showed that the addition of ELSF in making chips significantly affected of number of total LABs of chips (P <0.05) , however, there was not significantly effect provided to the existence of gram bacteria. Among the treatments (TO, T1 and T2), the Total LAB respectively 1.2 X102; 1.0 x103 and 2.3 x104. Regard to the score the presence of Gram +/- bacteria, among the treatments respectively 1.00; 1.70 and 1.90. It is considered that the product of chip had enough contained the gram postive bacteria i.e. the LAB , but the gram negative bacteria was still found.Key word : cassava meal; EFVW; LABs; Gram bacteria
PERUBAHAN KADAR RAFINOSA, GLUKOSA, MANOSA, ARABINOSA DAN SUKROSA WHEAT POLLARD AKIBAT LAMA STEAM DAN PENAMBAHAN AIR YANG BERBEDA Bambang Sulistiyanto; Sri Kismiati; Cahya Setya Utama
JURNAL LITBANG PROVINSI JAWA TENGAH Vol 15 No 2 (2017): Jurnal Litbang Provinsi Jawa Tengah
Publisher : Badan Perencanaan Pembangunan, Penelitian dan Pengembangan Daerah Provinsi Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36762/jurnaljateng.v15i2.412

Abstract

Wheat pollard is a waste of the wheat industry and many contain non starch polisacharida (NSP) that interfere with the chicken digestive tract. NSP in wheat pollard can be removed by heating, so it can be used by microbes in the digestive tract of poultry. Utilization of NSP by microbes will result in short chain fatty acids (SCFA), namely acetic acid, propionate and butyrate which have a positive effect on the digestive tract of poultry. The purpose of this study is to examine changes in levels of rafinose, glucose, mannose, arabinose and sucrose in wheat pollard into a source of functional feed for poultry. The benefit of research is to know the technique / manufacture of functional feed material from wheat pollard. The material used in the research is wheat pollard and aquades by using autoclave tool. The design used was a complete randomized design of 2x3 factorial pattern with 3 replications. The first factor is the duration of steam 30 and 60 minutes while the second factor is the addition of water (0, 30 and 60%). The results showed that the addition of water and the duration of different steam strongly influenced (p <0.001) and had interactions on rafinose, mannose, arabinose, sucrose and glucose parameters. The mean of water addition factor increased rafinose level from 1.11+0.03% to 29+0.02% while steam duration decreased raffinose content from 1.99+0.01% to 1.90+0.04%. Glucose levels increased in water addition factor and steam duration from 0.25+0.01% to 1.06+0.12% and 0.54+0.03% to 0.63+0.07%. The mean of water addition factor decreased the mannose level from 0.19+0.01% to 0.06+0.02% and the steam duration increased the manosa level from 0.09+0.01% to 0.15+0.01%. Levels of arabinose increased in water addition factor and steam duration from 0.30+0.03% to 0.98+0.01% and 0.54+0.02% to 0.70+0.04%. The average water addition decreased the sucrose content from 1.80+0.09% to 1.55+0.04% and the steam duration increased the sucrose content from 1.48+0.04% to 1.72+0.03%. The research conclusions that the steam treatment and the addition of water greatly affect the changes of rafinose, glucose, mannose, arabinose and sucrose in wheat pollard and potentially as functional feed. Recommendations that can be given by researchers is the source of feed ingredients that have high starch content should be processed before it is given to poultry so it has a functional value and improve the digestibility of the feed material.
PENGARUH PENAMBAHAN STARTER Lactobacillus Sp. TERHADAP NILAI pH DAN TOTAL ASAM LAKTAT SILASE RUMPUT Panicum maximum Sri Sumarsih; Bambang Sulistiyanto
JURNAL LITBANG PROVINSI JAWA TENGAH Vol 15 No 2 (2017): Jurnal Litbang Provinsi Jawa Tengah
Publisher : Badan Perencanaan Pembangunan, Penelitian dan Pengembangan Daerah Provinsi Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36762/jurnaljateng.v15i2.414

Abstract

The aim of the research was to evaluate the effect of Lactobacillus Sp. as starter on pH and total Lactic Acid of Panicum maximum Grass Silage. The completely randomized design was been used on this research with 4 treaments and 4 replications. The treatments were different level LAB (0,2,4 dan 6% v/w). The parameters were pH value and lactc acid. The results showed that pH value were significantly (p<0.05) decreased, and Lactic acid were significantly (p<0.05) with addded of Lactobacillus Sp. on Panicum maximum Grass Silage. The conclusion was the use of starter Lactobacillus Sp. on ensilage process produces chemical qualities of good Panicum maximum silage.
Profil Mikrobiologis Pollard yang Difermentasi dengan Ekstrak Limbah Pasar Sayur pada Lama Peram yang Berbeda Cahya Setya Utama; Bambang Sulistiyanto; Bhakti Etza Setiani
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.485 KB) | DOI: 10.17969/agripet.v13i2.816

Abstract

Profile microbiological of pollard fermented with extract of waste vegetable market in different long ripened ABSTRACT. The purpose of fermentation is to produce a product (material feed) that have nutritional content, texture and better biological availability, while it also can reduce the anti-nutritional. Microorganisms are often used as probiotics in feed is kind of Lactobacillus sp and Saccharomyces cerevisiae. Microorganisms are able to produce secondary metabolites such as β -glucan, mannan oligosaccharides and anti-cancer. Very familier as probiotic Lactobacillus among humans or livestock , while saccharomyces cerevisiae have specific characteristics in animal feed because of its ability to produce glutamic acid which can increase feed palatability. Grant Saccharomyces cerevisie can enhance digest protein and fiber, such as cellulose and hemicellulose , with Sacaromyces cerevisiea supplementation can increase the rate of short-chain fatty acids in cecum and suppresses the growth of bacteria from the Enterobacteriaceae species. Observing the above, needed an activity to find additional engineering efforts antibiotics as a source of natural probiotic , prebiotic and synbiotic on the particular poultry and livestock in general, to take advantage of the waste as a probiotic supplement that naturally produced feed additives to support healthy organic livestock production and economically.
Kandungan Total Bakteri dan Total Fungi pada Pellet Limbah Penetasan yang Dibuat dengan Penambahan Bentonit Aprilian Adi Nugroho; Sri Sumarsih; Bambang Sulistiyanto
Jurnal Agripet Vol 16, No 2 (2016): Volume 16, No. 2, Oktober 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.803 KB) | DOI: 10.17969/agripet.v16i2.3192

Abstract

ABSTRAK. Penelitian efek penambahan bentonit dalam proses pelleting terhadap total bakteri dan total fungi dari produk pellet limbah penetasan sebagai bahan pakan alternatif, telah dilaksanakan di Laboratorium Teknologi Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Penambahan bentonit pada proses pelleting diharapkan mengurangi total bakteri dan total fungi pada pellet olahan limbah penetasan, sehingga aman digunakan sebagai bahan pakan alternatif. Pada penelitian ini limbah penetasan yang berupa cangkang telur, telur infertile, telur gagal menetas, serta DOC afkir dan mati dihancurkan, dicampur dengan bentonit dan dibuat pellet, untuk kemudian dianalisis kandungan total bakteri dan total fungi pada produk pellet. Perlakuan bentonit dilakukan dengan aras 0, 2, 4 dan 6% (B/B). Penelitian dilakukan dengan rancangan acak lengkap (RAL) 4 perlakuan dan 5 ulangan. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji wilayah ganda Duncan untuk mengetahui perbedaan antar perlakuan. Hasil menunjukkan bahwa pemberian bentonit dari berbagai level berpengaruh sangat nyata (P0,01). Pemberian bentonit pada tingkat tertinggi (6%) mampu menekan total bakteri dan total fungi pada kisaran 105 cfu/g. Disimpulkan bahwa bentonit mampu menekan kandungan total bakteri dan total fungi pada produk pellet limbah penetasan. (Numbers of total bacteria and total fungi of hatchery waste pellets that made by adding bentonite) ABSTRACT. A Research about the effect of bentonite addition in the pelletizing process on total bacteria and total fungi of pellet product of hatchery waste as an alternative feedstuff, was done at Feed Technology Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University. The addition of bentonite was expected to reduce the number of bacteria and fungi in the pellet product of hatchery waste, therefore, it could be used safely as an alternative feedstuff. In this experiment, the hatchery waste in the forms of eggshell, infertile eggs, un-hatched eggs, dead and culled DOC were blended, mixed with bentonite and pelleted,and then the number of total bacteria and total fungi of pellet was analyzed. The treatment was carried out with the level of bentonite i.e. 0, 2, 4 and 6% (w/w). Completely randomized design (CRD)was applied by 4 treatments and 5 replications of each. Data analyzed by analysis of variance (ANOVA), and followed by Duncan multiple range test (DMRT) to determine differences among the treatments. The results showed that administration of bentonite at various levels significantly affect the number of bacteria and fungi in the hatchery waste pellets (P 0.01). Administration of bentonite at the level of 6% shows the highest value on suppressing the content of total bacteria as well as fungi up to the range of total content 105cfu/g. In conclusion, the bentonite was able to suppress the total amount of bacteria and total fungi in the hatchery waste pellets.
Pengaruh Penambahan Zeolit pada Proses Pelletizing Limbah Penetasan Terhadap Kandungan Coliform dan Salmonella Produk Pellet Bakhtiar Ali Wardana; Bambang Sulistiyanto; Sri Sumarsih
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.547 KB) | DOI: 10.17969/agripet.v16i1.3193

Abstract

ABSTRAK. Penelitian untuk mengkajipengaruh penambahan zeolit pada proses pelletizing limbah penetasan, terhadap total Coliform dan Salmonella,telah dilaksanakan di Laboratorium Teknologi Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Zeolit dilaporkan mampu mengikat berbagai senyawa kimia, termasuk senyawa beracun, serta mampu mempengaruhi aktivitas mikrobia. Penambahan zeolit dalam pelletizing limbah penetasan diharapkan mampu menurunkan total Coliform dan Salmonella dalam produk pellet, sehingga dapat meningkatkan keamanan produk pellet sebagai bahan pakan alternatif. Dosis penambahan zeolit pada pembuatan pellet yaitu: 0, 2, 4 dan 6%. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan penambahan zeolit dalam proses Pelletizing limbah penetasan berpengaruh sangat nyata (P0,01) terhadap penurunan total Coliform. Peningkatan dosis zeolite dapat menurunkan Total Coliform dan bakteri Salmonella tidak terdekteksi pada pellet limbah penetasan. Dapat disimpulkan bahwa pelletizing limbah penetasan dengan dosis zeolit hingga 6% terbuktimampu menurunkan kandungan Coliform dan Salmonella pada produk pellet.  (Effect administering zeolite in the pelletizing of hatchery waste to contents of coliform and salmonella of the pellet products) ABSTRACT. Experiment to study effect of administering zeolite in the pelletizing hatchery wastes on the total Coliform and Salmonella was done in the Laboratory of Feed Technology, Faculty of Animal Science and Agriculture, University of Diponegoro. Zeolite has been reported have binding capacity on wide range of chemicals, including toxic compounds, and interfere the activity of microbes. Administration zeolite in the pelletizing of hatchery wastes are expected to reduce the total Coliform and Salmonella in the pellet products, therefore improved the safety of the pellet products as an alternative feed ingredients. Doses of zeolite administration in the pelletizing are 0, 2, 4 and 6%. Data were analyzed by analysis of variance (ANOVA) followed by Duncan's multiple range test. The results showed, effect of administering zeolite in the pelletizing hatchery wastes was significant to the total Coliform (P 0.01). Increasing doses of zeolite reduced the Total Coliform and the Salmonella was not detected in the hatchery waste pellets. It could be concluded that pelletizing hatchery waste with a dose of zeolite of up to 6% proven to reduce the content of Coliform and Salmonella in the pellet products.
Jumlah Bakteri Asam Laktat (BAL) dalam Digesta Usus Halus dan Sekum Ayam Broiler yang Diberi Pakan Ceceran Pabrik Pakan yang Difermentasi Tri Setyo Widodo; Bambang Sulistiyanto; Cahya Setya Utama
Jurnal Agripet Vol 15, No 2 (2015): Volume 15, No. 2, Oktober 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.18 KB) | DOI: 10.17969/agripet.v15i2.2376

Abstract

(The amount of lactic acid bacteria in ileal digesta and secal digesta of broiler fed ration containing fermented abandoned feed)ABSTRACT. The study aims to examine the fermented feed quality in vivo against the amount of lactic acid bacteria in ileal and cecum digesta of broiler’s. The material used were 105 broiler DOC in 48,24±4,10g body weight. The experiment was conducted in completely randomized design (CRD) with 3 treatments and 6 replications. The treatments were T0 = Commercial Feed, T1 = spills Feed, T2 = fermented spills feed. Parameters measured were amount of lactic acid bacteria in the small intestine and cecum. Amount of lactic acid bacteria in small intestine and cecum were not normally distributed, was analyzed by Kruskal-Wallis test and followed by Mann-Whitney test. The results of study showed that treatments significantly affect (p0.05) the amount of lactic acid bacteria in the ileal digesta. Feeding fermented spills feed significantly increased amount of lactic acid bacteria in ileal digesta. The use of fermented spills feed not affect the amount of lactic acid bacteria in the cecum digesta. It is concluded that feeding fermented spills feed had a positive effect on broiler by increasing the amount of lactic acid bacteria in the ileal digesta 
Total Jamur dan Identifikasi Yeast pada Limbah Kubis Fermentasi dengan Penambahan Vitamin dan Mineral Cahya Setya Utama; Bambang Sulistiyanto; Kezia Naomi Christy Ginting
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 3 (2020): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.661 KB) | DOI: 10.33772/jitro.v7i3.12194

Abstract

ABSTRAKPenelitian bertujuan mengkaji peningkatan kualitas limbah kubis fermentasi dengan penambahan vitamin dan mineral dilihat dari kandungan total jamur dan identifikasi yeast. Metode penelitian menggunakan rancangan acak lengkap (RAL) pola searah dengan 5 perlakuan dan 3 ulangan. Pengolahan data pada parameter total jamur diolah menggunakan anova, jika ada pengaruh yang nyata dilanjutkan uji duncan, sedangkan pada parameter identifikasi yeast menggunakan metode deskriptif. Penelitian menunjukkan bahwa penambahan vitamin dan mineral berpengaruh nyata (P<0,05) terhadap total jamur yang dihasilkan. Total jamur terbanyak pada perlakuan penambahan vitamin dan mineral 10% dengan rata-rata total jamur 71,33 x 104 CFU/g. Pengecatan gram pada identifikasi jenis yeast didapatkan bentuk oval, soliter dan gram positif (yeast) menunjukkan identifikasi jenis yeast yang tumbuh yaitu Saccharomyces cerevisiae. Simpulan penelitian adalah penambahan vitamin dan mineral sebanyak 10% mampu meningkatkan kualitas limbah kubis fermentasi dilihat dari kandungan total jamur dan jenis yeast.Kata kunci: fermentasi, jamur, limbah kubis, pengecatan gram, yeastABSTRACTThe study aimed to evaluate the quality improvement of fermented cabbage waste which was added by vitamins and minerals by observing the total fungus and type of yeast. The method used was a completely randomized design (CRD) unidirectional pattern, with 5 treatments and 3 replications. Statistical analysis of total fungus was processed by ANOVA and continued by the Duncan Multiple Renge test, and the data type of yeast was discussed descriptively. The result showed, the addition of vitamin and mineral treatment significantly influenced the number of fungi (P<0,05). The highest number of fungus was shown beside 10% supplementation of vitamins and minerals (7.33 x 104 CFU/g). Gram coloring on the identification of yeast types obtained oval, solitary, and gram-positive (yeast) it shows that the identification of the growing yeast type, Saccharomyces cerevisiae. The conclusion of the addition of 10% vitamins and minerals can improve the quality of 2 days cabbage waste fermentation seen from the total fungus and the type of yeast that grows.Key Words: cabbage waste, fermentation, gram paintings, yeast
Co-Authors Alfian Mustofa Anum D Aprilian Adi Nugroho Bakhtiar Ali Wardana Bhakti Etza Setiani C Imam Sutrisno Cahya Setya Utama Cahya Setya Utama Cahya Setya Utama Cahya Setya Utama Cahya Setya Utama Cahya Setya Utama Cahya Setya Utama Enik Sukarti Istiyan PM Kezia Naomi Christy Ginting Marry Christiyanto Muhammad Fikri Haidar Muhammad Fikri Haidar Neneng Nurhalimah, Neneng Nurwantoro . Oktavianus Barus Oktavianus Barus Saroh, Siti Yuni Sholikhul Mahfudi Sri Kismiati Sri Kismiati, Sri Sri Mukodiningsih Sri Sumarsih Sri Sumarsih Tri Setyo Widodo Ulfah, Mariana Rusida Wicaksono, T. A. Widiyanto Widiyanto