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Response of Chicken that Having Experience Infection of Ascaridia galli to Re-infection and it’s Implication to Productivity and Quality of Eggs Zalizar, L; Satrija, F; Tiuria, R; Astuti, DA
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The aimed of this research was to find out the effect of infection experience of Ascaridia galli on productivity and eggs qualities. The research was held in Helminthology Laboratory, Veterinary Faculty and Animal Production Laboratory, Faculty of Animal Husbandry, Bogor Institute of Agriculture. The research was based on a Randomized Completely Design.  The treatments were P0 = without infection ; P1 = have been infected with  200 infective  eggs A. galli every chick every week ( 8, 15, 22 and 30 days old chick) and re-infected with 500 infected eggs at 18 weeks old; P2= chicks with  no infection experience at starter period, and infected with 500 infected eggs at laying period. The productivity and quality of eggs were examined. The results showed that infection experience of Ascaridia galli influenced the layer productivity and their eggs qualities. The experience of A. galli infection several times with light dosage at starter period (P1) made the layers more resistance to re-infection by the parasite in the laying period. Consumption and conversion of feed, eggs weight, shell thickness and calcium concentration of P1 was not significant difference with control group (P0). First A. galli infection in layer period in group without experience of A. galli infection before (P2), have showed that, compare with the control group (P0), the feed conversion of P2 was 15.78% higher (P<0.01), eggs weight of P2 was lighter 5.35% (P<0.05), the shell thickness of P2 eggs was lower 5.55% (P<0.05), the calcium concentration in serum was lower 36.26% (P<0.05). Beside that the color of eggs yolk in infected (P1 and P2) group more colorless (11.63%) than control group. A. galli (P<0.01). Ascaridia galli infection has no effects on Haugh Unit Value, titer serum protein and eggs protein. (Animal Production 9(2): 92-98 (2007) Key Words : Infection experience, Ascaridia galli, productivity of layer, eggs qualities
Efektivitas Daun Jarak (Jatropha curcass Linn) Sebagai Anticacing Ascaridia galli dan Pengaruhnya terhadap Performa Ayam Lokal S Suharti; K G Wiryawan; R Tiuria; Y Ridwan; S Fitriana; N Sumarni
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.885 KB) | DOI: 10.5398/medpet.2010.33.2.108


The present experiment was aimed to identify the phytochemical of Jatropha curcas leave extracted with water and methanol as an anthelmintic agent for Ascaridia galli, and its effect on native chicken performance. In vitro study of anthelmintic activity was conducted by counting the number of paralyzed worm dead-body of A. galli during 18 hours in petri dish containing different levels of extract, namely 0%, 1%, 2%, 3%, and 4% (w/v) and compared to the piperazine 0.5% (w/v). Eightteen birds of naturally A. galli-infected native chicken were used for the in vivo study. The treatments were 0%, 2%, 4%, 8%, and 16% of J. curcass leave extract, and 10% of piperazine using a completely randomized block design with 6 treatments and 3 replications. Parameters observed were fecal worm egg count, feed consumption, body weight gain, feed conversion ratio, and mortality. The results showed that water- and methanol-extracted J. curcas leave had similar composition of secondary metabolite compounds which is high in triterpenoid and steroid contents, respectively. Percentage of paralyzed A. galli was higher (P < 0.01) in water-extracted jatropha leaves. On the contrary, the dead-body percentage was higher (P < 0.05) in the methanol-extracted than that in the control group. In vivo study showed that leave meal significantly decreased (P < 0.05) fecal worm egg count. The leaf meal at the level 16% tended to increase feed consumption, body weight gain, and significantly decreased feed conversion ratio. In conclusion, J. curcas leave meal have anthelmintic activity to A. galli and could improve nutrient utilization of naturally A. galli-infected native chicken by decreasing feed conversion ratio.
The Ability of Immunoglobulin Yolk Recognized the Antigen in the Tissue of Ascaridia galli . Darmawi; U Balqis; M Hambal; R Tiuria; B P Priosoeryanto; E Handharyani
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1489.954 KB) | DOI: 10.5398/medpet.2012.35.3.190


Antigen-antibody reaction is an important tool for the analysis of localization of target molecules, including antigenic protein within worm tissues. The purpose of the present research was to demonstrate the ability of immunoglobulin yolk (IgY) anti-excretory/secretory recognized the antigen in the tissue of Ascaridia galli by mean of immunohistochemistry method. The excretory/secretory protein was procured from A. galli and concentrated by mean of vivaspin 30,000 MWCO. IgY was produced by egg yolks of immunized chickens with excretory/secretory, and purified using fast protein liquid chromatography (FPLC) method. A. galli adult worms were cut in transversal and longitudinal section of the center and anterior region. Slides were incubated with both primary IgY for overnight at 4 oC and secondary antibody rabbit anti-chicken IgY HRP-conjugate for one hour at room temperature. The slides were stained with 3-amino, 9-ethylcarbazole (AEC) chromogen, counterstained with Lillie Mayer Haematoxylin, and mounted in glyserin aqueous mount.  Antigen-antibody reaction was investigated under a microscope. The result showed that antigen was appeared in the tissues such as cuticle, epicuticle, buccal cavity, and eggs inside the uterine of A. galli. This research concluded that IgY stimulated by the excretory/secretory was able to recognized the antigen scattered in the tissues of A. galli so the IgY could be applied for immunodiagnostic.