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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Perikanan dan Kelautan The Journal of Experimental Life Sciences (JELS) Jurnal Sains dan Teknologi Pangan Science and Technology Indonesia Jurnal Sumberdaya Akuatik Indopasifik Jurnal Perikanan Universitas Gadjah Mada Khadimul Ummah Journal of Social Dedication Indonesian Food Science and TechnologyJournal FaST : Jurnal Sains dan Teknologi Asian Journal of Aquatic Sciences
Hardoko Hardoko
Fakultas Perikanan Universitas Brawijaya Malang.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum Hardoko Hardoko; Bambang B. Sasmito; Yunita E. Puspitasari; Hamid M. Afandi; Nugroho Maulia
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1225.92 KB) | DOI: 10.21776/ub.jels.2017.007.02.10

Abstract

Shrimp shells are one of chitin sources in Indonesia which is potential to be converted into multifunctional glucosamine. This research was aimed to study the glucosamine production by fermentation using Trichoderma harzianum.  Method used was experimental fermentation with pH treatment of 3-5 and fermentation duration of 10-20 days, and designed using Response Surface Methodology (RSM). Results showed that fermentation duration of 10-20 days has reached the optimum point of glucosamine production from shrimp shells using T. harzianum. The highest production of D-glucosamine in fermentation using T. harzianum occurred on initial pH of 5.41 and fermentation duration of 15 days (18,294.95 ppm), while the highest N-Acethyl-D-Glucosamine production occurred on initial pH of 3.00 and fermentation duration of 20 days (127,000.00 ppm).Keywords: fermentation, glucosamine, shrimp shells, T. harzianum.
In Silico Analysis of Phenolic Compounds from Ceriops decandra Griff. Leaves and Molecular Interaction as Anti Diabetes Yunita Eka Puspitasari; Mochamad Arega Alfikri; Romasni Sitanggang; Jeny Ernawati Tambunan; Hardoko Hardoko
Science and Technology Indonesia Vol. 8 No. 4 (2023): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2023.8.4.542-553

Abstract

α-amylase and α-glucosidase in the gastrointestinal tract have an important role in the hydrolysis α-1,4 and α-1,6 glycosidic chain of starch, respectively. Inhibition of both enzyme activities becomes one of the strategies to control diabetes. However, commercial drugs such as antidiabetics have adverse effects such as gastrointestinal problems. Therefore, exploring functional food, especially from marine natural products as antidiabetic agents, is potential. In particular, Ceriops sp. was reported to contain bioactive compounds with antidiabetic properties, but its mechanism to treat diabetes has not been proved. The potency of phenolic compounds of C. decandra leaves as α-amylase and α-glucosidase inhibitors were examined in this research by implementing the molecular docking analysis in silico. Three steps of analysis were carried out in this study, including extraction from C. decandra leaves with different polarity solvents, identification of phenolic compounds using LC-HRMS, and molecular docking analysis of phenolic compounds identified from C. decandra leaves. This study revealed that quercetin, rutin, epicatechin, isorhamnetin, caffeic acid, and ferulic acid were identified from C. decandra leaves. According to the drug-likeness and toxicity analysis, the presented compounds in C. decandra leaves had high potential pharmacological properties. Furthermore, molecular interaction analysis exhibited phenolic compounds extracted with ethyl acetate, such as quercetin and epicatechin, and with methanolic extracts, such as quercetin, rutin, epicatechin, and isorhamnetin, were more effective as α-amylase and α-glucosidase inhibitors than from caffeic acid and ferulic acid. Among the phenolic compounds of C. decandra leaves, rutin and quercetin were predicted to be the potential α-glucosidase inhibitors.
PENENTUAN KONDISI FERMENTASI DALAM PRODUKSI N-ASETILGLUKOSAMIN DARI KULIT UDANG MENGGUNAKAN BAKTERI Providencia stuartii Yuniwaty Halim; Ratna Handayani; Shella Trey Lamtoro; Hardoko Hardoko
Jurnal Sains dan Teknologi Pangan Vol 8, No 5 (2023): Jurnal Sains dan Teknologi Pangan
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v8i5.43422

Abstract

Penelitian ini dilakukan untuk menentukan jenis substrat, pH, suhu, dan lama fermentasi terbaik untuk menghasilkan N-asetilglukosamin oleh bakteri Providencia stuartii. Bakteri Providencia stuartii yang digunakan merupakan hasil isolasi dari penelitian sebelumnya. Kondisi fermentasi terbaik ditentukan dengan melakukan fermentasi pada berbagai suhu (32oC, 37oC, dan 42oC), pH (5, 6, 7, dan 8), serta lama fermentasi. Hasil penelitian menunjukkan bahwa konsentrasi N-asetilglukosamin tertinggi dengan menggunakan substrat tepung cangkang udang dihasilkan pada suhu fermentasi 37oC dengan pH media 7 dan lama fermentasi 9 hari, yaitu sebesar 178.752,670 ± 1.774,478 ppm. Sedangkan konsentrasi N-asetilglukosamin tertinggi dengan menggunakan substrat kitin dihasilkan pada suhu fermentasi 37oC dengan pH media 8 dan lama fermentasi 4 hari, yaitu sebesar 68.789,00 ± 2682,65 ppm.
Co-Authors Alifah Nur Fajrin Alifiah Nur Fajrin Anies Chamidah Arning Wilujeng Ekawati Bambang B. Sasmito Bambang Budi Sasmita Clara Tasia Dean Rama Prayoga Dela Rosa Delvira Riska Ananta Eddy Suprayitno Halim, Yuniwaty Hamid M. Afandi Handayani, Ratna Heder Djamaluddin Heder Djamaludin Hezkiel Oktorully Tampubolon Hezkiel Oktorully Tampubolon Jacinta Fanya Setiadi Jenny Ernawati Tambunan manik, ria retno Mikchaell Alfanov Pardamean Panjaitan Mochamad Arega Alfikri Muhammad Dailami Natania Kam Nugroho Maulia Reinald Febryanto Pengalila Romasni Sitanggang Shella Trey Lamtoro Tambunan, Jeny Ernawati Tian Nur Ma’rifat Titik Dwi Sulistiati Titik Dwi Sulistiyati Titri Siratantri Mastuti Vivi Nurhadianty Yunita E. Puspitasari Yunita Eka Puspitasari