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All Journal EKSAKTA: Journal of Sciences and Data Analysis Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Biomolecular and Health Science Journal Best Journal (Biology Education, Sains and Technology) Indonesia Berdaya I-Com : Indonesian Community Journal
Rofiatun Solekha
Biology Program, Faculty Of Science, Technology, And Education, Universitas Muhammadiyah Lamongan, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Analysis Metallothionein of Carp fish in The Brantas River, Indonesia Putri Ayu Ika Setiyowati; Rofiatun Solekha; Sri Bintang Sahara; Febianti Dwi Hapsari
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 1, ISSUE 2, August 2020
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol1.iss2.art8

Abstract

This study aimed to detection of protein profile, expression of metalothionein (MT) protein, and analyze difference of MT density in liver and gill in one of Carp fish that is Barbonymus balleroides in the upstream and downstream of Brantas river. The method used observasional analytic, Two individual of Barbonymus balleroides samples taken three times (with difference of month) from two station there are upstream and downstream of Brantas river. Analysis protein profile used electrophoresis SDS-PAGE (15%), expression and density of MT used western blot method and imageJ software. Difference between MT density in liver and gill of Barbonymus balleroides in the upstream and downstream of Brantas river analyzed with Two-way ANOVA. The results showed, protein profile in liver and gill Barbonymus balleroides in the upstream and downstream Brantas river have molecular weight about 8-93 kDa, expression of MT showed band of MT with molecular weight 24 kDa, results of MT density in liver and gill on upstream and downstream, continuously 231.29 MT/µm2 and 229.87MT/µm2, 232.41 MT/µm2 and  231.56 MT/µm2 but there is not significant.
Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2 Putri Ayu Ika Setiyowati; Rofiatun Solekha; Sri Bintang Sahara Mahaputra Kusuma Negara; Reny Rosalina
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.26619

Abstract

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.
Protective Effect of Lemongrass Extract (Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate Putri Ayu Ika Setiyowati; Rofiatun Solekha; Dwi Dianita Irawan; Reny Rosalina; Karin Alifia Rachmadani; Angella Ananda Saputra
Biomolecular and Health Science Journal Vol. 5 No. 1 (2022): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v5i1.31450

Abstract

Introduction: Male infertility can be caused by many factors. One of which is environmental pollution such as lead acetate. Lead acetate exposure can increase Reactive Oxygen Species (ROS), affecting spermatozoa quality. Natural antioxidants and flavonoid on Lemongrass (C. nardus) have a great potential for protecting the male reproductive system. This study aimed to analyze the effect of giving various dosages of C. nardus extract on the sperm quality of mice. Methods: The Balb/C male mice were divided into five equal groups. The negative control group was injected with 0.01 ml Na-CMC 0.5% within 40 days, and the positive control group was injected with 0.01 ml lead acetate 7 mg/kg BW within 5 days, the treatment groups were injected by 0.01 ml lead acetate 7 mg/kg body weight within 5 days and continuously injected by 0.01 ml of C. nardus extract with various dosage 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW within 35 days. At the end of the experiment, mice were sacrificed, and sperm suspensions were collected from cauda epididymal to measure the morphology, concentration, and motility.Results: The results showed that giving C. nardus extract could repair morphology, concentration, and motility of spermatozoa with significantly different (p<0.05). The 25 mg/kg BW dose has a good protective effect. Conclusion: The C. nardus extract can repair the decreasing sperm quality caused by lead acetate exposure.
Efek Proteksi Ekstrak Cymbopogon nardus pada Histologi Testis Mencit (Mus musculus) Setelah Induksi Timbal Asetat Lilis Maghfuroh; Putri Ayu Ika Setyowati; Rofiatun Solekha; Khoirin Nisa; Nynda Ayu Nadira Savitri
Buletin Anatomi dan Fisiologi Volume 7, Nomor 1, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.1.2022.20-26

Abstract

Penelitian ini bertujuan untuk mengetahui efek perlindungan ekstrak serai wangi Cymbopogon nardus (C. nardus) terhadap induksi timbal asetat. Sebanyak 25 mencit jantan strain BALB/C secara acak dibagi menjadi lima kelompok. Kelompok kontrol negatif hanya mendapat injeksi sub cutan Na-CMC 0,5% selama 30 hari ; kontrol positif  diinjeksi sub cutan timbal asetat sebanyak 7 mg/kg bb selama 5 hari; P1, P2, dan P3 secara berturut-turut diinjeksi sub cutan timbal asetat 7 mg/kg bb selama 5 hari kemudian dilanjutkan dengan injeksi ekstrak C. nardus sebanyak 25, 50, dan 100 mg/kg bb selama 25 hari. Pada akhir penelitian semua mencit dikorbankan dan dilakukan koleksi testis. Jaringan testis diproses menggunakan pewarnaan Hematoxylin-Eosin. Hasil menunjukkan bahwa terjadi peningkatan sel spermatogonia, sel spermatosit, dan sel spermatid serta diameter tubulus seminiferus dibandingkan dengan kelompok positif. Dosis optimal yang paling mampu memperbaiki kerusakan jaringan testis yaitu 25 mg/kg bb. This study aimed to determine the protective effect of Cymbopogon nardus (C. nardus) extract against lead acetate exposure. A total of 25 male mice BALB/C were randomly divided into five groups. The negative control group only received 0.5% Na-CMC subcutaneously for 30 days; positive control was given 7 mg/kg bw of lead acetate subcutaneously for 5 days; P1, P2, and P3 were sequentially injected with 7 mg/kg bw subcutaneously for 5 days followed by injection of 25, 50, and 100 mg/kg bw of C. nardus extract for 25 days. At the end of the study all mice were sacrificed and testes were collected. Testicular tissue using Hematoxylin-Eosin staining. The results showed that there was an increase in spermatogonia cells, spermatocytes, and spermatid cells as well as the diameter of the seminiferous tubules compared to the positive group. The optimal dose most capable of repairing testicular tissue damage was 25 mg/kg bw.  
Phytochemical Screening of Ethanol Extract on Stems, Leaves and Roots of Citronella Grass (Cymbopogon nardus L) Rofiatun Solekha; Putri Ayu ika Setiyowati; Sri bintang Sahara Mahaputra Kusumanegara; Chandra Tri uliana Sari
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5320

Abstract

Citronella grass is a plant that belongs to the rhizome group. In its application, lemongrass is widely used for both food, medicine and aromatherapy needs. The various benefits of lemongrass are interesting to research about the content of compounds in lemongrass. The purpose of this study is to provide information about screening for chemical compounds in the ethanol extract of citronella (Cimbopogon nardus L). This research method is by way of Phytochemical Screening test, namely Alkaloids with Mayer, Dragendorff, and Bouchardat reagents. Flavonoid test with Shinoda test, tannin test with gelatin test, saponin test with foam test, terpenoid/steroid test with Liebermann-Burchard reagent. The results showed that phytochemical screening showed the presence of several compounds in the serum, namely alkaloids, tannins, saponins, triterpenoids and flavonoids in every member of the plant body, namely stems, roots and leaves, but did not contain steroids in the roots, stems and leaves.
Uji Ketahanan dan Total Alkaloid Tembakau (Nicotiana tabaccum) Setelah Infeksi Ralstolnia solanacearum Rofiatun Solekha; Putri Ayu Ika Setiyowati; Dimas Arya Nugraha; Karin Alifia Rachmadani
BEST Journal (Biology Education, Sains and Technology) Vol 4, No 1 (2021): Juni 2021
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v4i1.3588

Abstract

Ralstolnia solanacearum is solanacearum is non forming, gram negative, aerobic plant pathogen that causes wilting in various host plant. Ralstolnia solanacearum is non these bacteria cause wilt disease in tabacco plants which can cause death by up to 50%. In tobacco plants, resistance analysis in the form of alkaloid content is required, the use of alkaloids in plants is as protection form pest attacks, plant reinforcements and hormone regulators. This study aims to analyze the resistance after developing tobacco wilt disease after Ralstolnia solanacearum infection. This study used kemloko 2 and kemloko 3 varieties as resistant treathments, kemloko 1 varieties which were susceptible to being used as negative controls, and moderately resistant Sindoro 1 varieties as positive controls. Reliability analysis using IP and AUDPC than the alkaloid cntent analysis using chloroform. The result showed the kemloko 3 variety tobacco had 103, 40 value; kemloko 2 11,74; sindoro 1 205,76; and kemloko 1 has value of AUDPC 350,22. Kemloko 3 is the most resistant variety after Ralstolnia solanacearum infection.  The result of the analysis showed that the highest total alkaloid levels were found in kemloko 3, namely 15,760 ± 0,51 mg equivalent to the value of caffeine / gram. This shows that there is a correlation between the resistance of a plant to the many alkaloid compounds in the plant. The more resistant, the more lacloid compound content.  
Inventarisasi Bryophyta, Pteridophyta, Gymnospermae Di Kabupaten Lamongan Dwi Zulianti; M. Ainul Mahbubillah; Nynda Ayu Nadira Savitri; Rofiatun Solekha
BEST Journal (Biology Education, Sains and Technology) Vol 4, No 1 (2021): Juni 2021
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v4i1.3596

Abstract

Indonesia has rich of plant species because of its habitat variation. Bryophyta, Pteridophyta, and Gymnospermae mostly grow on highland habitat. Height of the habitat causes change of micro-climate, especially humidity. Lamongan District of East Java Indonesia Jawa Timur is a lowland area so that difficult to find species of those three taxa. For educational purposes, data collection of the 3 taxa species that grow in Lamongan District is needed. The research was carried out by identifying plants Bryophyta, Pteridophyta, and in Lamongan District. The results of the study found that there were 3 Bryophyte species: Marchantia polymorpha, Polytrichum sp, and Riccia sp.; 3 Pteridophyte species: Pteridium aquilinum, Adiantum philippens, and Telmatoblechnum indicum; and 2 Gymnosperm species: Cycas rumphii, and Gnetum gnemon.   
Pelatihan Budidaya Maggot dengan Memanfaatkan Sampah Organik Hasil Pemilahan di Kelurahan Blimbing, Lamongan Rofiatun Solekha; Fika Nur Fitriana Putri Bukhori; Silvi Wasi’atul Af'idah; Lailatus Fitri; Aisyah Hadi Ramadani
I-Com: Indonesian Community Journal Vol 2 No 3 (2022): I-Com: Indonesian Community Journal (Desember 2022)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.368 KB) | DOI: 10.33379/icom.v2i3.2001

Abstract

Pemilahan dan pengolahan sampah masih menjadi cara yang dibutuhkan masyarakat untuk mengurangi masalah lingkungan. Pemanfaatan sampah organic menjadi salah satu produk yang bernilai ekonomis menjadi salah satu solusi yaitu pemanfaatan sampah untuk pakan dalam budidaya maggot black soldier fly (BSF). Tim PHP2D Prodi Biologi Universitas Muhammadiyah lamongan memberikan solusi dengan memanfaatkan sampah organic untuk pakan budidaya maggot dalam upaya mengurai permasalahan di Kelurahan Blimbing, Kecamatan Paciran, Kabupaten Lamongan. Tujuan dari program ini adalah memberikan pendampingan dan pelatihan guna pembedayaan msyarakat dalam mengolah sampah organik bernilai eknonomis. Metode pelaksanaan kegiatan pengabdian ini diawali dengan penyuluhan atau sosialisasi, kemudian dilanjut dengan diadakannya pelatihan bersama dengan masyarakat setempat dalam pembuatan instalasi serta teknis budidaya black soldier fly (BSF). Hasil dari kegiatan ini adalah terciptanya produk maggot lalat black soldier fly (BSF) dalam produk kemasan siap jual. Seluruh kegiatan pelatihan berjalan dengan sukses. Proses budidaya telah melalui satu siklus hidup BSF dan dihasilkan produk maggot BSF yang siap untuk dipasarkan. Monitoring dan evaluasi program menunjukkan adanya timbal balik yang positif dari peserta.
Training on Separation of Organic and Inorganic Waste in Blimbing Village, Paciran Lamongan District Rofiatun Solekha; Nynda Ayu Nadira Savitri; Karin Alifia Rachmadani; Badriatul Musyarofah; aisyah hadi ramadani
Indonesia Berdaya Vol 4, No 2 (2023)
Publisher : Utan Kayu Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.2023455

Abstract

Waste problems are becoming increasingly complex due to the lack of waste processing facilities, low public knowledge about waste management, and the rapid population growth in Paciran District, Lamongan Regency. This activity is carried out with the aim of utilizing it by sorting waste and processing it so that it can produce products. Trash that is scattered on the road is generally due to the low awareness of the surrounding community to dispose of waste in its place. Community service activities are carried out by socialization and training. The socialization carried out is to provide counseling to the community about how to dispose of waste and sorting household waste. Training was conducted for TPS3R officers on how to sort and use them. Organic waste obtained from food scraps is used as maggot feed, while inorganic waste is sold to collectors so that it can increase people's income in the Blimbing village. Apart from that, this activity also produced a BSF (Black Soldier Fly) installation, which is a place for maggot cultivation. It is hoped that in the future this community service activity can be carried out in a sustainable manner, so that the waste in the Blimbing sub-district can be utilized into something of economic value.
Penyulingan Minyak Atsiri Serai Wangi Dengan Metode Stabilitas Suhu dan Lama Penyulingan untuk Meningkatkan Rendemen Rofiatun Solekha; Putri Ayu Ika Setiyowati; Badriyatul Musyarofah; Salasun Nisah; Mufti Ari Bianto; Bagus Dwi Jauhari
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 1 (2023): Juni 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i1.6519

Abstract

Serai wangi merupakan tanaman yang mampu menghasilkan minyak dengan nilai jual tinggi. Pemilihan Teknik dan metode yang tepat dalam proses penyulingan serai wangi perlu diperhatikan untuk meningkatkan rendemen dari serai wangi. Suhu dan lama penyulingan akan berpengaruh terhadap banyaknya rendemen yang akan didapatkan dalam satu kali penyulingan. Penelitian ini bertujuan untuk mengetahui optimalisasi suhu dan lama penyulingan terhadap banyaknya rendemen yang didapatkan. Metode yang digunakan yaitu metode destilasi dengan 2 faktor yaitu factor suhu dan lama penyulingan. Suhu yang digunakan dalam proses penyulingan yaitu 90oC dan 100oC dan lama penyulingan  90 menit, 120 menit dan 150 menit.  Hasil destilasi penyulingan minyak atsiri serai wangi yang menghasilkan rendemen terbanyak adalah penyulingan dengan menggunakan suhu 100oC dan waktu 120 menit yaitu menghasilkan rendemen 45% pada penyulingan daun serai wangi seberat 15 kilo. Sifat fisik tertinggi dinilai dengan harga densitas rendemen yaitu 0,9067 g/ml pada  suhu 110oC yaitu dan waktu 120 menit. Semua variabel dalam penelitian memenuhi standar SNI dengan pengukuran indeks bias. Tekanan destilasi dan lama penyulingan mempengaruhi banyaknya rendemen serta sifat fisik rendemen
Co-Authors Aisyah Hadi Ramadani Aji Naufal Syafiq Angella Ananda Saputra Angella Ananda Syaputra Badriatul Musyarofah Badriatul Musyarofah Badriyatul Musyarofah Bagus Dwi Jauhari Chandra Tri uliana Sari Dimas Arya Nugraha Dwi Dianita Irawan Dwi Zulianti emil Milhatul Maflahah Halma Febianti Dwi Hapsari Fika Nur Fitriana Putri Bukhori Karin Alifia Rachmadani Karin Alifia Rachmadani Karin Alifia Rachmadani Khoirin Nisa Lailatus Fitri Lilis Maghfuroh Mahbubillah, M. Ainul Mufti Ari Bianto Nur Khurotul A’in Nynda Ayu Nadira Savitri Nynda Ayu Nadira Savitri Putri Ayu Ika Setiyowati Reny Rosalina Salasun Nisah Silvi Wasi’atul Af'idah Sri Bintang Sahara Sri Bintang Sahara Mahaputra Kusuma Negara Sri bintang Sahara Mahaputra Kusumanegara