Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Pertanian Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI Jurnal Ilmiah Sains
UTUT WIDYASTUTI
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Immunology & microbiology Mathematics Physics Veterinary

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Hubunagn kemampuan pergantian inang dengan plastisitas genetika pada cendawan blas padi (Pyricularia grisea) Sri Listiyowati; Utut Widyastuti; Gayuh Rahayu; Alex Hartana; Muhammad Jusuf
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1504.563 KB)

Abstract

The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P. grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Bali and Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolates Dc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1.
Introduction of the Serine Green Fluorescent Protein (sGFP) Gene into Pyricularia grisea Race dc4 Isolated from Digitaria ciliaris using Agrobacterium tumefaciens-mediated Genetic Transformation Stephanie Stephanie; Utut Widyastuti; Suryo Wiyono
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 22 No. 1 (2015)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.329

Abstract

Gene serin Green Fluorescent Protein (sGFP) has been used to monitor gene expression specific tagged proteins that has implication for fungal cell study. This research aimed to introduce sGFP gene into genome of P. grisea dc4 from D. ciliaris using A. tumefaciens. Plasmid sGFP was introduced into A. tumefaciens by triparental mating method (TPM). Genetic transformation was performed by co-cultivating spore P. grisea dc4 with A. tumefaciens LBA4404–pCAMB-sGFP. Pyricularia grisea dc4 transformant was selected by using selection medium that contains 300 µg/ml hygromycin. The integration of sGFP gene into genome was confirmed by PCR using  sGFP’s spesific primer pair, sGFP-Nos terminator primer pair and β-Tubulin primer pair as internal control. Expression of sGFP from P. grisea dc4 transformant were detected with blue light fluorescent microscope.
Binary Vector Construction and Agrobacterium tumefaciens-mediated Transformation of Lysozyme Gene in Seaweed Kappaphycus alvarezii Tri handayani; Alimuddin Alimuddin; Utut Widyastuti; Emma Suryati; Andi Parenrengi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 21 No. 2 (2014)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.348

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Dwi Susilaningsih; Asahedi Umoro; Fredrick Onyango Ochieng; Dian Noverita Widyaningrum; Hani Susanti; Hadi Susilo; I Nengah Swastika; Utut Widyastuti
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ‘Carita’ Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
PERTUMBUHAN TANAMAN JARAK PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P Gedoan; Alex Hartana; Hamim Hamim; Utut Widyastuti; Nampiah Sukarno
JURNAL ILMIAH SAINS Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.582 KB) | DOI: 10.35799/jis.11.2.2011.205

Abstract

PERTUMBUHAN TANAMAN JARAK  PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P. Gedoan1), Alex Hartana2), Hamim2), Utut Widyastuti2) dan Nampiah Sukarno2) 1)Program Studi Biologi, FMIPA Universitas Negeri Manado di Tondano; 2)Departemen Biologi, FMIPA, Institut Pertanian Bogor, Bogor 16680   ABSTRAK Kegiatan penambangan timah menyebabkan perubahan karakteristik fisika dan kimia tanah sehingga menjadi tidak sesuai untuk pertumbuhan tanaman.  Tujuan penelitian ini untuk mempelajari pertumbuhan 7 aksesi jarak pagar yang ditanam pada lahan pasca tambang timah yang diberi kompos dan kotoran sapi.  Penelitian ini dilakukan di TSS 133, Kelurahan Sinar Baru, Kabupaten Bangka, Provinsi Bangka Belitung.  Penelitian lapangan dilaksanakan pada Bulan Mei 2007 sampai dengan Bulan Desember  2008.  Percobaan faktorial ini dirancang dalam Rancangan Petak Terbagi dengan tiga ulangan.  Petak utama adalah 7 aksesi jarak pagar yang terdiri atas: aksesi Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu,  dan Sukabumi, sedangkan  anak petak berupa tanah tanpa pemberian kompos dan pupuk kandang (kontrol), kompos trubus 4 kg/lubang ditambah 4 kg tanah bagian atas  dan  kotoran sapi 4 kg/lubang ditambah 4 kg tanah bagian atas. Hasil penelitian menunjukkan bahwa pertumbuhan awal yang paling baik untuk tanah bekas tambang timah adalah pada aksesi Jember yang ditunjukkan oleh diameter batang, berat kering tanaman, berat kering tajuk dan berat kering akar.  Sedangkan tinggi tanaman tertinggi diamati pada aksesi Madiun.  Penambahan kotoran sapi dapat meningkatkan produksi biji dan kandungan minyak.  Produksi biji tertinggi diperoleh pada aksesi Bengkulu dan kandungan minyak tertinggi diperoleh pada aksesi Dompu. Kata kunci: aksesi, Jatropha curcas, kompos, kotoran sapi   THE GROWTH OF CASTOR OIL PLANT (Jatropha curcas L.) ON THE POST-TIN-MINING LAND IN BANGKA PROVIDED WITH ORGANIC FERTILIZER ABSTRACT The activity of tin mining changes the physical and chemical characteritics of soil, so that the soil isnot suitable for plant growth.  The objective of this research was to evaluate the growth of some accession of Jatropha curcas that we planted on post-tin-mining land provided with compost and cow feces.   This research was conducted in TS 133, Sinar Baru Village, District of Bangka, Province of Bangka Belitung.  This field research was carried out in May 2007 to December 2008.  Factorial experiment was designed as split plot with three replications.  The main plot was 7 accessions, i.e. Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, and  Sukabumi.  The subplot waskonds of organic fertilizer, i.e. top soil without addition of compost and cow fecer (control), trubus compost  (4 kg/hole) added with top soil (4 kg/hole), and cow feces (4 kg/hole) added with topbsoil (4 kg/hole). The result showed that Jember accession had the best early growth based on the observation of stem diameter, plant dry weight, and root dry weight.  The largest plant height was observed in Madiun accession.  The higest seed production was observed in Bengkulu accession and Dompu accession had the higest oil content.Keywords: accession, Jatropha curcas, , compost, cow feces
Co-Authors Alex Hartana Alimuddin Alimuddin Andi Parenrengi Asahedi Umoro Dian Noverita Widyaningrum Dwi Susilaningsih Emma Suryati Fredrick Onyango Ochieng Gayuh Rahayu Hadi Susilo Hamim Hamim Hani Susanti I Nengah Swastika Muhammad Jusuf Nampiah Sukarno Sri Listiyowati stephanie stephanie Sukmarayu P Gedoan Suryo Wiyono Tri Handayani