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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH JSDH Jurnal Biologi Tropis Makara Journal of Science
Sri Listiyowati
Departmen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Hubunagn kemampuan pergantian inang dengan plastisitas genetika pada cendawan blas padi (Pyricularia grisea) Sri Listiyowati; Utut Widyastuti; Gayuh Rahayu; Alex Hartana; Muhammad Jusuf
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1504.563 KB)

Abstract

The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P. grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Bali and Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolates Dc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1.
Keragaman Cendawan Pascapanen pada Umbi Bawang Merah Varietas Bima Brebes Okky Setyawati Dharmaputra; Sri Listiyowati; Ira Zahara Nurwulansari
Jurnal Fitopatologi Indonesia Vol 14 No 5 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.217 KB) | DOI: 10.14692/jfi.14.5.175

Abstract

Diversity of Postharvest Fungi on Shallot Bulbs Variety Bima BrebesIn Indonesia, shallot (Allium ascalonicum) is horticultural main commodity after hot pepper. Significant yield losses can be caused by postharvest fungi infection. Research on the diversity of postharvest fungi on shallot bulbs has been conducted in some countries, unfortunately little is done in Indonesia. The study was aimed to obtain information on the diversity of postharvest fungi infecting shallot bulbs variety Bima Brebes from several traditional markets in Bogor City. Shallot bulbs were collected in January and February 2016. The study consisted of fungal isolation from shallot bulbs, fungal pathogenicity test, and identification of pathogenic fungi based on morphological and molecular characteristics. Morphology identification was based on the color of fungal colony, growth pattern, as well as somatic and reproduction structures. Several species of pathogenic fungi were successfully identified from shallot bulbs i.e. Alternaria alternata, Aspergillus niger, Colletotrichum gloeosporioides species complex, Fusarium fujikuroi species complex, F. oxysporum, F. solani, Penicillium citrinum and P. pinophilum.  Among these fungi, the highest pathogenicity was shown by C.  gloeosporioides species complex.
Potensi Cendawan Asal Hydrilla verticillata Sebagai Pengendali Hayati Fusarium oxysporum dan Ganoderma boninense: Fungi from Hydrilla verticillata as Potential Biological Control Agents of Fusarium oxysporum and Ganoderma boninense Astri Ariyani; Nampiah Sukarno; Sri Listiyowati
Jurnal Sumberdaya Hayati Vol. 5 No. 2 (2019)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.5.2.43-51

Abstract

Hydrilla verticillata is an aquatic weed, can build symbiosis and also become a substrate of freshwater fungi. Some of freshwater fungi that live in association with H. verticillata are pathogenic fungi which were potentially used as controller for Fusarium oxysporum and Ganoderma boninense. The use of pathogenic fungi as a biocontrol is not consistent in the field. Therefore, this research aimed to find new fungus candidates that are stronger and more consistent in controlling pathogenic fungi. Freshwater fungi were isolated from H. verticillata. Identification was based on morphological characters. Molecular analysis was carried out for freshwater fungi which had the highest inhibition percentage to pathogenic fungi. The antagonism test was conducted by dual culture method. The mechanism of interaction of hyphae was observed under a microscope. Freshwater fungi was derived from H. verticillata stem are Aspergillus section flavi, Gliomastix sp., and Talaromyces sp. Antagonism test results showed that all fungi could inhibit the growth of pathogenic fungi. Based on molecular analysis used ITS region showed Talaromyces sp. could not identify to the level of species so that additional genes were required, namely beta-tubulin gene, in order to further identify Talaromyces sp.
Diversity of SCAR Markers of Pyricularia grisea Isolated from Digitaria ciliaris Following Cross Infection to Rice SRI LISTIYOWATI; UTUT WIDYASTUTI; GAYUH RAHAYU; ALEX HARTANA; MUHAMMAD JUSUF
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.5.1.1

Abstract

Cross infection of Pyricularia grisea from grass to rice and vice versa has been reported, but genetic changes are not known yet. This research aimed at estimating the possibility of the genotype alteration in P. grisea dc4 isolated from Digitaria ciliaris, following cross infection to either rice cv. Kencana bali, Cisokan, and IR64 or Panicum repens, Cynodon dactylon, Digitaria sp., and Ottochloa nodosa. The genotypes were analyzed by employing three SCAR markers, Cut1; PWL2; and Erg2. The results indicated that the dc4 was only able to infect Kencana bali, Cisokan, and P. repens. The dc4 had only two out of three SCAR markers, Cut1 and Erg2. Host shift was followed by genotype alteration in two loci of SCAR. Isolates derived from lesions on Kencana bali (dc4-kb) and Cisokan (dc4-c) of the dc4 infection, both lost their Cut1 and gained PWL2. On the contrary, there was no genotype alteration from dc4 to isolate derived from P. repens of dc4 infection (dc4-pr). Neither the isolate dc4-kb that was cross-inoculated to Cisokan nor the dc4-c that was cross-inoculated to Kencana bali showed SCAR marker change. In comparison, race 173 isolate and those derived from Kencana bali and Cisokan did not show genotype alteration. All had two out of three SCAR markers, PWL2 and Erg2. The isolate 173 was adapted to rice. This indicated that genotype diversity of the dc4 might arise following host shift from grass to rice.Pyricularia grisea merupakan cendawan blas yang telah diketahui memiliki kisaran inang luas selain pada padi. Infeksi silang cendawan blas pada rumput ke padi dan sebaliknya telah dilaporkan, tetapi perubahan genetiknya belum dilaporkan. Tujuan penelitian ini menganalisis kemampuan infeksi silang dan perubahan genotipe P. grisea dc4 asal Digitaria ciliaris dalam perpindahannya ke padi cv. Kencana bali, Cisokan, dan IR64 atau rumput Panicum repens, Cynodon dactylon, Digitaria sp. dan Ottochloa nodosa. Genotipe P. grisea dianalisis melalui tiga marka SCAR, yaitu Cut1; PWL2; dan Erg2. Isolat dc4 memiliki 2 marka SCAR, yaitu Cut1 dan Erg2; tidak memiliki PWL2. Isolat dc4 hanya mampu menginfeksi silang Kencana bali, Cisokan, dan P. repens. Turunan isolat dc4 sebagai hasil infeksi silang ke Kencana bali (dc4-kb) dan Cisokan (dc4-c) menunjukkan perubahan genotipenya, yaitu Cut1 tidak teramplifikasi pada keduanya; PWL2 teramplifikasi;, serta Erg2 tetap teramplifikasi. Sebaliknya, turunan isolat dc4 sebagai hasil infeksi silang ke P. repens (dc4-pr) tidak mengalami perubahan genotipe. Turunan isolat dc4-kb sebagai hasil infeksi silang ke Cisokan, maupun turunan isolat dc4-c dari Kencana bali, juga tidak menunjukkan perubahan genotipe, yaitu tetap menunjukkan keberadaan PWL2 dan Erg2. Sebagai pembanding digunakan isolat ras 173 yang diisolasi dari padi. Genotipe isolat tersebut maupun turunannya, sebagai hasil infeksi silang ke Kencana bali dan Cisokan, tidak menunjukkan perubahan. Perubahan genotipe dc4 terjadi mengikuti pergantian inang dari rumput ke padi.
Identification of Aspergillus flavus and Detection of Its Aflatoxin Genes Isolated from Peanut and Peanut Processed Products Kemala S. Nagur; Nampiah Sukarno; Sri Listiyowati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 21 No. 1 (2014)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.864 KB) | DOI: 10.11598/btb.2014.21.1.398

Abstract

Aspergillus flavus is one of the main fungi that are able to produce aflatoxin.The presence of the fungi and its aflatoxin are become serious problem on food safety. This research was aimed to isolate and identify A. flavus from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis used species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R, while detection of  aflatoxin genes employed four specific primers of apa-2 (aflR), nor-1 (aflD), ver-1 (aflM) and omt-1 (aflP). From 36 samples, the A. flavus group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10-4 cfu/g. The total 18 isolates were identified as A. flavus based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp respectively. Based on aflatoxin gene analysis showed that all 18 isolates successfully amplified by both apa-2 and nor-1, 83.3 % by omt-1 and 72.2 % by ver-1 genes which taking part in aflatoxin production. The amplicons size of apa-2, nor-1, ver-1 and omt-1   primer pairs were about 1032, 400, 895 and 1024 bp respectively.
Yeasts Isolated from Traditional Brem Bali Show Stress Tolerance Phenotype against Fermentation-Related Stresses Lenka, Audria Bayu; Astuti, Rika Indri; Listiyowati, Sri
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

The search for microbes, primarily yeasts with unique characters such as the tolerance against fermentation-related-stresses, is gaining significant interest nowadays. Traditionally made alcoholic beverages can be used as sources for such yeasts, given that during fermentation and storage, microbes may develop stress tolerance responses leading to naturally stress-tolerant yeast strains. In this study, we used an alcoholic beverage, that is, Brem Bali, as the source of potential yeast isolates. We isolated nine yeast isolates from two traditional Brem Bali products. All isolates showed tolerance against high glucose stress (40–50%) and sensitivity against high-temperature stress (37–50 °C). Notably, isolate BT5 showed tolerance phenotype against ethanol stress (up to 12.5%). Notably, the ethanol stress tolerance phenotype shown by isolate BT5 was unlikely correlated to the ability of the isolates in combating other stresses. Based on the internal transcribed spacer sequence, isolates BT2, BT5, and BT6 shared high similarity with Wickerhamomyces anomalus (98%). Further approaches may be needed to clarify the identity of these isolates. Based on our study, isolate BT5 bears potential as a fermentation agent based on its ability to combat high glucose and ethanol stresses.
Diversitas Pangan Fermentasi Berbasis-Susu di Indonesia dan Kandungan Gizinya Firyal Noviatanti Nabilah; Sri Listiyowati; Rika Indri Astuti
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 4 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.4.552

Abstract

Indonesia has abundant and diverse food commodities, including fermented food. However, metadata analysis about the diversity of fermented foods has not been reported. In addition, a comparison of the nutritional content of milk-based fermented foods is also not yet available. Therefore, this study aims to reveal the diverse status of fermented foods, to determine the nutritional content, especially of fermented milk-based foods, namely dangke, and their comparisons between milk-based fermented products. The research method included literature study, making dangke, proximate analysis of dangke, and statistical data analysis. Literature studies show that fermented foods and beverages in Indonesia were most reported in 2017, 2018, and 2019 respectively. Although most fermented foods are not known for their area of origin, the microbial groups involved in their processing come from the bacterial group rather than fungi, mainly through the lactic acid fermentation mechanism. Microbes' role in fermented products is diverse and can be grouped into five groups. The primary role of microbes is to support bioprocesses. Among milk-based fermented products, dangke is one of the traditional products with a fat content that is significantly different fat content from yogurt, kefir, and dadih, and the protein is not significantly different from yogurt, kefir, dadih, and mozzarella cheese. This nutritional content analysis indicates differences in fat and protein content among fermented milk-based food products. Keywords: dangke, fat content, metadata, protein content, proximate analysis
Monascus sp. Pigment Potency as Simple Preservative in Food Ingredients Wahyu Aji Mahardhika; Maulida Aqlinia; Delfiani Anggias Putri; Firda Sri Efendi; Vincentia Fenice Angger Maherani; Sri Listiyowati
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.3761

Abstract

Everyday foodstuffs such as tofu, chicken, and fish are ingredients that have a short shelf life if stored outdoors or in the open air. The fungus Monascus sp. is a fungus whose pigment is often used in giving color to food. This study aims to determine the potential of Monascus sp pigment isolated from commercial red rice (Angkak) as a preservative in tofu, chicken, and fish at room temperature. The methods used included isolation of the fungus Monascus sp from commercial red rice (Angkak), macroscopic and microscopic morphological characterization, solid fermentation using rice, harvesting pigments with water solvent, and pigment resistance tests on tofu, chicken meat, and fish. The results obtained from the application of the crude extract of the Monascus sp. pigment in fish survived for two days, chicken meat lasted up to three days, and tofu lasted four days. Each experiment had different results during the organoleptic test and compared to a positive control, namely turmeric, proving that turmeric was still better at resisting food spoilage. This research needs to be investigated further regarding the potential if the pure extract of the Monascus sp pigment is used.
Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae Nadila Fany Ashafani; Rika Indri Astuti; Sri Listiyowati; Irmanida Batubara
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.734-742

Abstract

Metabolites from clove (Syzygium aromaticum L.) have been extensively studied for various pharmacological uses, including quercetin. Endophytic bacteria isolated from plant tissues can produce similar secondary metabolites as their host plants. However, little is known about the antioxidant activity of the endophytic bacterial extract from clove leaves. Amongst the potential bacterial groups, the genera of Bacillus have been widely studied as a source of antioxidants. Thus, this study, we examined the potential antioxidant properties of Bacillus sp., isolated from clove leaves. We have successfully isolated ten endophytic bacteria, of which only one isolate (P1U2) was found non-pathogenic based on a hemolytic assay. Based on the 16S rRNA sequence, P1U2 shared the highest similarity to Fictibacillus phosphorivorans. The extract of extracellular metabolites of P1U2 contains quercetin compounds, based on LC-MS analysis. The results of the antioxidant activity test using the DPPH assay showed that the P1U2 extract had an IC50 value of 161 mg/ml. The supplementation of the extract on culture media reduced the ability of S. cerevisiae to decolorize mutagen malachite green and increased cell viability. Furthermore, the addition of 161 ppm extract was able to counteract UV mutagens effects of UV exposure and toxicity of malachite green, significantly increasing the growth of S. cerevisiae. Suggesting the bioactivity of the extract in combating mutagens. To the best of our knowledge, this is the first study to report a quercetin-rich extract derived from endophytic bacteria, F. phosphorivorans. Thus, further study is required to optimize the synthesis of quercetin from isolate P1U2.
Antagonistic Mechanism of Entomopathogenic Fungi Against Fusarium oxysporum f. sp. cubense, The Causal Agents of Banana’s Panama Disease Sri Listiyowati; Tya Rustiani; Gayuh Rahayu
Jurnal Fitopatologi Indonesia Vol 19 No 3 (2023)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.3.99-110

Abstract

Fusarium oxysporum f. sp. cubense merupakan cendawan tular tanah penyebab penyakit panama pada tanaman pisang. Agens hayati dari kelompok cendawan telah banyak dilakukan untuk mengendalikan penyakit tanaman. Oleh sebab itu, penelitian ini bertujuan mengidentifikasi empat koleksi cendawan entomopatogen berdasarkan pada ciri morfologinya dan mengevaluasi mekanisme antagonismenya terhadap F. oxysporum f. sp. cubense IPBCC 19 1472. Galur cendawan entomopatogen dengan kode PS 4, PS 9, PS 11 berasal dari kawasan Situ Gunung, Sukabumi, Jawa Barat dan galur KRC berasal dari Kebun Raya Cibodas, Jawa Barat. Identifikasi cendawan dilakukan berdasarkan ciri morfologi pada medium agar-agar dekstrosa kentang. Mekanisme antagonistik diteliti menggunakan metode biakan ganda dan sebagai kontrol digunakan biakan tunggal F. oxysporum f. sp. cubense. Pengamatan dilakukan terhadap daya hambat cendawan entomopatogen dan pertumbuhan koloni F. oxysporum f. sp. cubense. Semua cendawan entomopatogen tidak dapat diidentifikasi secara morfologi karena tidak bersporulasi. Pertumbuhan koloninya lebih lambat daripada F. oxysporum f. sp. cubense. Semua cendawan entomopatogen menghambat F. oxysporum f. sp. cubense melalui mekanisme kompetisi ruang. Galur KRC memiliki aktivitas antagonisme paling besar, diikuti berturut-turut oleh galur PS 9, PS 11, dan PS 4. F. oxysporum f. sp. cubense membentuk klamidospora sebagai respons terhadap cendawan entomopatogen galur PS9 dan vakuolisasi ketika berinteraksi dengan tiga galur cendawan entomopatogen lainnya.
Co-Authors ALEX HARTANA Alex Hartana Astri Ariyani Cici Rahmawati Delfiani Anggias Putri Firda Sri Efendi Firyal Noviatanti Nabilah Gayuh Rahayu Gayuh Rahayu Handika Dwi Prasetyo Ira Zahara Nurwulansari Irmanida Batubara Irmanida Batubara Kemala S. Nagur Lenka, Audria Bayu Maulida Aqlinia MUHAMMAD JUSUF Muhammad Jusuf Nadila Fany Ashafani Nampiah Sukarno Nampiah Sukarno Okky Setyawati Dharmaputra Rika Indri Astuti Rika Indri Astuti Rika Indri Astuti Tya Rustiani UTUT WIDYASTUTI UTUT WIDYASTUTI Vincentia Fenice Angger Maherani Wahyu Aji Mahardhika Wendi Nurul Fadillah Yanti Novera