Article Per Year (5 Year)
p-Index From 2019 - 2024
4.805
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Ethos Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Folium : Jurnal Ilmu Pertanian Berkala Penelitian Hayati AGRONISMA
Novi Arfarita
Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

Published : 40 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 40 Documents
Search

 1   2   3   4 
ISOLATION AND CHARACTERISTIC OF NITROGEN-FIXING BACTERIA AND PHOSPHATE-SOLUBILIZING BACTERIA FROM SOIL HIGH IN MERCURY IN TAILINGS AND COMPOST AREAS OF ARTISANAL GOLD MINE Nuraini, Yulia; Arfarita, Novi; Siswanto, Bambang
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research was conducted at Brawijaya University and West Nusa Tenggara, from March 2013 to October 2013. The tailings areas of the gold mine contains high mercury (Hg) as much as 1,090 ppm, and living microbes (resistance) exist in a small number in such a condition. Microbial P solvents encountered came from the genus Bacillus with a population of 23 x 103 cfu g-1 and N-fixing bacteria encountered are of the genus Bacillus, with a population of 4 x 103 cfu g-1. Identification of species using Becton Dickinson Phoenix test, both species belong to Bacillus megaterium and Bacillus pumilus. While the waste corn and peanuts that has been composted for 4 weeks acquired P-solubilizing bacteria, such as Enterobacter, Bacillus and Pseudomonas, and N-fixing bacteria found was Pseudomonas and Azotobacter. While testing the activity and antagonism of N-fixing and P-solubilizing bacteria, the result on agar media did not show antagonism in its growth. Bacillus pumilus and Bacillus megaterium effective at 5 % molasses medium with the number of 0.15 x 1012 on seven days of incubation. Keywords: N-fixing bacteria, P-solubilizing bacteria, mercury, phytoremediation
THE APPLICATION OF Trichoderma viride STRAIN FRP 3 FOR BIODEGRADATION OF GLYPHOSATE HERBICIDE IN CONTAMINATED LAND Arfarita, Novi; Djuhari, Djuhari; Prasetya, Budi; Imai, Tsuyoshi
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.550

Abstract

In this current study, we observed Trichoderma viride strain FRP3 capability for biodegradation of glyphosate on contaminated land in Indonesia. There were two blank plots that have been involved as representatives of indigenous fungal, that prepared as control (non-contaminated soil) and P1 (GP-contaminated soil) while the treatments were represented by two plots.  Plot 2 (P2) was introduced with conidia suspension of Trichoderma viride strain FRP3 one time application, and plot 3 (P3) was introduced with conidia suspension of Trichoderma viride FRP3 two time applications. At the end of observation, the CFU of two times application was the highest with CFU of 15.97 x 106 gr-1 soil. The CFU of P3 was corresponding to 45% higher than P2 (8.83 x 106 gr-1 soil). The CFU of GP-contaminated soil without conidia suspension application had 0.66 x 106 gr-1soils, only 0.7% and 0.4% corresponding to P2 and P3, respectively. Direct indicator of glyphosate degradation was determined using GC analysis. Within 7 days after Trichoderma viride FRP3 was introduced, glyphosate content of treated soil decreased. This fungal strain provided 48% (P2) and 70% (P3) of glyphosate degradation higher than indigenous soil microbial community (P1) within 28 days of application.
OBSERVASI EFEK EKSTRAK ETANOL DAUN BAMBU JAWA (Gigantochloa atter (Hassk.) Kurz) DENGAN PARAMETER FISIK DAN FISIOLOGI HEWAN UJI TIKUS (Rattus sp.)YANG DIINDUKSI BORAKS Airlangga, Hardadi; Safitri, Endand; Arfarita, Novi
El-Hayah : Jurnal Biologi Vol 5, No 2 (2015): EL-HAYAH (VOL 5, NO 2, Maret 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i2.3023

Abstract

Bamboo Jawa leaves (Gigantochloa atter) is known to contain antioxidant compound sandhas been used as abeverage from generation to generation invarious Asiancountries. While in Indonesia, currently borax widely abusedas a food preservative and food additives. Borax produce exogenous free radicals that can lead to organ damage due to oxidative stress.This current study was to observe the effect of ethanolic extract of G. atter leaf using physiology and physical parameter onthe levels of SOD, MDA and the hepatosite necrosis totalof rats induced by borax. Borax will generate free radicals which cause cell damage through lipid peroxidation there by increasing the levels of MDA and lower SOD antioxidant. Borax metabolism occurs mostlyin theliver, therefore, one of the organs damaged by borax is liver The method of this research was experimental control group posttest only using wistar rats which divided into five groups. The negative control group was induced by aquades. The positive control group was induced by borax 1gr/kg BW and treatment group was induced by borax. Ethanolic extract of G. atter leaf was given with doses of 10mg/ml, 20mg/ml and 40mg/ml for 21 days. After 21 days of treatment, hepatocite necrosis was then observed and counted using microscope trinocular 1000x. SOD examination was conducted by the NBT test while the MDA using TBA test. Data analysis was using one way ANOVA test. G. atter ethanolic extract dosageof 10 mg/ml, 20 mg/ml and 40 mg/mlcould decrease the number of liver cells necrosis. They were 23%, 39% and 63% respectivelly when compared to positive control. Extract ethanolik of G.Atter leaf also could improve SOD and decrease MDA rats serum which induced by borax. The effective dose is 20mg/ml.
ISOLASI DAN IDENTIFIKASI BAKTERI PENGHASIL PROTEASE YANG DISKRINING DARI TERASI ARFARITA, NOVI
El-Hayah : Jurnal Biologi Vol 5, No 3 (2015): EL-HAYAH (VOL 5, NO 3,September 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i3.3098

Abstract

Terasi is a traditional fermented shrimp paste of Indonesia popularly used as an important ingredient in many Indonesian cuisines, and further screened for their protease-producing bacteria. A total of 117 bacterial isolates were retrieved  from 4 terasi samples. When inoculatedusing skim milk agar, 71 isolates (~61%) produced distinct clear zones implying their proteolytic activity. A bacterial isolate  namely Bacillus S2-3 wasselected for further study due to its widest clear zones when thebacterial cells and culture supernatant were used. This strain could grow up to 60oC with a broad pH range of 5 – 9. The optimal growth condition was at pH 6 and 37oC. The enzyme activity was also characterized using azocasein method. The optimal pH of bacterialenzymes was between 6 and 7 whereas the optimal temperature of BacillusS2-3 proteases was 22 – 24oC. For thermostability, the S2-3 enzymes remained active up to 2h at 40 and 50oC. The results ofbiochemical tests, BacillusS2-3 was identified as Bacillussubtilis, later explored it is potential for the purposes for  food industry
Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment Novi Arfarita; Anton Muhibuddin; Tsuyoshi Imai
Journal of Degraded and Mining Lands Management Vol 6, No 2 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.22 KB) | DOI: 10.15243/jdmlm.2019.062.1617

Abstract

Nitrogen is the most abundant element in the atmosphere, however, most often deficient in agricultural lands. This research was an exploratory to get indigenous non-symbiotic nitrogen-fixing bacteria. Soil samples were collectedfrom rhizosphere of green beans. This study was aimed to determine the bacterial population of the three regions; screening, isolation and selection of free nitrogen-fixing bacteria. Antagonism and pathogenicity tests were performed to observe its potential for a biofertilizer product. The highest number of free nitrogen-fixing bacteria was found from forest soil sample of 2.5x 1011CFU/ml. Screening and isolation process has obtained 10 free nitrogen-fixing isolates. Then was selected into 4 isolates namely SNF4, SNF5, SNF7 and SNF8 according to the ammonia production test qualitatively. When an antagonism activity performed, there was no inhibition zone each other. The pathogenicity test did not show the pathogenic symptom. This study also showed that bacterial isolates obtained significantly affected the germination growth of green beans compared to controls. Possibility, bacteria of this type produced growth hormone for a plant. Strain SNF8 has shown the highest ammonium production then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SNF8 had 99% similarity with Bacillus cereus.
Effects of seaweed waste on the viability of three bacterial isolates in biological fertilizer liquid formulations to enhance soil aggregation and fertility Novi Arfarita; Takaya Higuchi; Cahyo Prayogo
Journal of Degraded and Mining Lands Management Vol 6, No 4 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2019.064.1889

Abstract

Biofertilizer production in Indonesia should fulfil the minimum requirement for being produced and released to the market. Problems occurred when those products are being absent on informing those expiration dates and the viability of microbial activity which then closely related to the quality of the product. Seaweed composted material are potential resources for producing Biofertilizer, but lacking on the optimization on their process as this material contain a various important component for soil and environment. The production of Biofertilizer from seaweed waste required an optimum condition, i.e.: pH and typical microbe which could germinate under specific formulation and temperature. This study aimed to determine the optimum pH in liquid fertilizer formulations made from seaweed waste in the form of composted material, to test the viability of three bacterial isolates and those pathogenicity properties, to examine the effect of metabolites release from bacterial isolates to green bean seed germination. The experimental design used was a completely randomized design with four treatments, which were as follow: P0 as a control (Peptone), RP1 (seaweed waste), RP2 (seaweed waste and glycerol), and RP3 (seaweed waste and PEG). The three bacterial isolates used were: (1) Bacillus licheniformis, (2) Psudomonas plecoglossicida and (3) Pantoea ananatis. This liquid fertilizer biological formulation was stored for 8 weeks at pH 5.5 and temperature 25oC. The results showed that the treatment of RP1 (seaweed waste) had high bacterial viability and could stimulate growth for green bean sprouts. The carrier material for seaweed waste with the addition of glycerol and PEG showed no effect of the disease and symptoms of a pathogenic bacterial consortium on germination of green beans.
Penerapan Teknologi "BioPot" dalam Menunjang Revegatasi Lahan Bekas Tambang Pasir Novi Arfarita; Cahyo Prayogo
ETHOS (Jurnal Penelitian dan Pengabdian) Vol 8 No.2 (Juni, 2020) Ethos: Jurnal Penelitian dan Pengabdian kepada Masyarakat (Sains & Teknologi
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/ethos.v8i2.5920

Abstract

Abstract. The community-managed sand mining activity in Bambang Village, Wajak District, Malang Regency caused serious environmental descruptions and many did not have official permits. The land that was once fertile turned into degraded land, the level of soil fertility, often found former sand dug pits and cliffs that have relatively steep slope. Joint land rehabilitation includes land use management, erosion and sedimentation control, and revegetation and maintenance of introduced plants. Land structuring is carried out to prepare land to become land ready for planting by adopting "BioPot" technology which is preparing planting hole on the ground consisting of a mixture of clay, manure, biofertilizers and lime. Selected plants adapt to existing conditions consisting of woody plants and fruits such as: Pule (Alstonia macrophylla), Kecrutan (Spatodea campalunata), Bungur (Langerstroemia spesiosa), Avocado (Persea americana), Pete (Parkia speciaosa), Durian (Spatodea campalunata), Pucuk merah (Syzygium myrtifolium), etc. Based on the existing land conditions, the land is arranged using a blocking system and between plants 4 m x 4 m apart. This technology can be adopted well by the community and planted plants can grow and develop properly.Keywords: Land Former Sand Mining, Revenge, Biofertilizer, Pioneer Plants Abstrak. Kegiatan penambangan pasir yang dikelola masyarakat di Desa Bambang, Kecamatan Wajak, Kabupaten Malang menyebabkan kerusakan lingkungan yang serius dan banyak yang tidak memiliki ijin resmi. Lahan yang semula subur berubah menjadi lahan terdegradasi tingkat kesuburan tanahnya, banyak dijumpai lubang bekas galian pasir serta tebing yang memiliki kemiringan lereng relatif curam. Rehabilitas lahan yang dilakukan bersama mencakup penataan lahan, pengendalian erosi dan sedimentasi, serta revegetasi dan pemeliharaan tanaman yang diintroduksikan. Penataan lahan dilakukan untuk menyiapkan lahan menjadi lahan siap tanam dengan mengadopsi teknologi “BioPot” yang merupakan lubang tanam terdiri dari campuran tanah liat, pupuk kandang, biofertiliser dan kapur. Tanaman terpilih menyesuaikan dengan kondisi yang ada yang terdiri dari tanaman kayuan dan buah-buahan seperti: Pule (Alstonia macrophylla), Kecrutan (Spatodea campalunata), Bungur (Langerstroemia spesiosa), Alpukat (Persea americana), Pete (Parkia speciaosa), Durian (Durio zibhentinus), Pucuk merah (Syzygium myrtifolium), dll. Berdasarkan kondisi lahan yang ada, lahan ditata menggunakan sistem blocking dan antar tanaman berjarak 4 m x 4 m. Teknologi ini dapat diadopsi dengan baik oleh masyarakat dan tanaman yang ditanaman dapat tumbuh dan berkembang dengan baik. Kata Kunci : Lahan Bekas Tambang Pasir, Revegetasi, Biofertilizer, Tanaman Pioneer
Pemanfaatan Eceng Gondok (Eichornia Crassipes) Dan Limbah Pertanian Untuk Persediaan Pakan Ternak Dan Biokompos Berbasis Teknologi Fermentasi Novi Arfarita; Anik Malikah; Djuhari - Djuhari
ETHOS (Jurnal Penelitian dan Pengabdian) Vol 10 No.1 (Januari, 2022) Ethos: Jurnal Penelitian Dan Pangabdian Kepada Masyarakat (Sains & Tekno
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/ethos.v10i1.6859

Abstract

Water hyacinth causes environmental problems in the Selorejo Reservoir due to its rapid growth. This activity aims to introduce fermentation-based technology to farmer groups (klomtan) in the use of water hyacinth as animal feed and compost, reduce environmental impact and increase its economic value. The methods used are (1) Organizing education and training on zero waste product technology through the manufacture of silage and bio compost for water hyacinth weeds; (2) Provide a special place for the manufacture of silage and bio compost; (3) Strengthening klomtan institutions with production and marketing management training; (4) Assist in production monitoring. This program has achieved the following targets: (1) Farmers and ranchers can adopt a zero-waste technology package to increase livestock productivity and agricultural products; (2) Increasing the awareness and ability of klomtan to utilize agricultural waste and water hyacinth weeds; (3) Institutional strengthening of the Karya Makmur and Lestari Jaya Makmur groups so that they can become examples of farming communities in their villages. The results of this activity showed an increase in the knowledge of group members by 85% of the technology offered. Continuous assistance needs to be carried out because fermentation technology is influenced by various factors so that it is not easily applied by klomtan. Eceng gondok menyebabkan masalah lingkungan di Waduk Selorejo karena pertumbuhannya yang cepat. Tujuan kegiatan ini untuk mengenalkan teknologi berbasis fermentasi kepada kelompok tani (klomtan) dalam pemanfaatan Enceng Gondok sebagai pakan ternak dan kompos, mengurangi dampak lingkungan dan meningkatkan nilai ekonominya. Metode yang digunakan adalah (1) Melakukan penyuluhan dan pelatihan teknologi zero waste product dengan memberikan pelatihan pembuatan silase dan biokompos gulma enceng gondok; (2) Menyediakan tempat khusus pembuatan silase dan biokompos; (3) Penguatan kelembagaan klomtan dengan pelatihan manajemen produksi dan pemasarannya; (4) Melakukan pendampingan monitoring produksi. Kegiatan ini telah mencapai target: (1) Petani dan peternak mampu mengadopsi paket teknologi zero waste product untuk meningkatkan produktivitas ternak dan hasil pertanian; (2) Tumbuhnya kesadaran dan kemampuan klomtan untuk memanfaatkan limbah pertanian dan gulma enceng gondok; (3) Penguatan Manajemen Kelembagaan klomtan Karya Makmur dan Lestari Jaya Makmur agar dapat menjadi contoh masyarakat tani-ternak di desanya. Hasil kegiatan ini menunjukkan peningkatan pengetahuan anggota kelompok sebesar 85% terhadap teknologi yang ditawarkan. Pendampingan secara terus-menerus perlu dilakukan karena teknologi fermentasi dipengaruhi oleh berbagai faktor sehingga tidak mudah diterapkan oleh klomtan.
Enhancing bare land soil quality using electric induction apparatus in combination with rabbit urine liquid fertilizer application to support garlic (Allium sativum) production Novi Arfarita; Mohammad Jasa Afroni; S Sugiarto; Tsuyoshi Imai
Journal of Degraded and Mining Lands Management Vol 7, No 4 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.074.2381

Abstract

The new approach in this study was using electric induction technique to enhance soil fertility of bare land in a combination of rabbit urine application in the form of liquid fertilizer. This approach had been exaggerating soil microbial activities and modifying the exchange capacity of anions and cations in soil. This study aimed to find out the best combination of different duration of electricity induction and rabbit urine liquid fertilizer at different levels of their application. Randomized factorial block design with two factors was used for this study. The first factor was the duration of the electric induction at the rates of 0, 30, 45, and 60 minutes, while the application of rabbit urine as liquid organic fertilizer was at the rates of 0, 100, 150, and 200 mL/L. Plant height, number of leaves and stem diameter, fresh and dry weight of garlic bulb were measured within 3 months. The observations were carried out using destructive and non-destructively approached with intervals of 7 days. The best treatment that gave the highest plant height, number of leaves, stem diameter, tuber diameter, fresh and dry garlic bulb and garlic production was found under the combination of 60 minute of electricity induction and 200 mL/L of urine rabbit application (P<0.05). Also, this treatment was successfully to give garlic production of 8.56 t/ha.
Structure and Composition of Major Arbuscular Mycorrhiza (MA) under Different Farmer Management of Coffee and Pine Agroforestry System Cahyo Prayogo; Dimas Prastyaji; Budi Prasetya; Novi Arfarita
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2639

Abstract

Utilization of Arbuscular Mycorrhiza (MA) as beneficial soil microbes is expected to support nutrient demand for improving crop performance. However, under the agroforestry system that facing a problem on light, water, and nutrients competitions, the role of MA is becoming unclear. The purpose of this research was to examine how far different management in Coffee Pines Agroforestry System (CPAS) affects MA structure and compositions. The relationship between soil parameters (e.g. pH, soil organic C) and MA activities was also being evaluated. The selected plot according to existing management practices were chosen as follows: (1) Low management (LC); (2) High management which then compared to (3) Business As Usual (BAU) plot in which were repeated in triplicate. ANOVA and multivariate analysis were employed to determine the effect of the treatments. The result showed that there were significant differences (p<0.05) in the structure and composition of the MA, in terms of the total number of MA spores and the abundance of Glomus sp. under the coffee tree sampling point, while the lowest number was detected in Gigaspora sp. genera. The more intensive land management resulted in a higher abundance of MA biomass which then leads to increased soil P and uptake-P along with MA infection.
Co-Authors adinda widya rahmawati Agus Sugianto Amanda Reha Zahran Anik Malikah Anis Rosyidah Anton Muhibuddin aprilia dwi nastiti ayu azhariah baltar ardyah pramesti Bambang Siswanto Bambang Siswanto Budi Prasetya Budi Prasetya Cahyo Prayogo Cahyo Prayogo Cahyo Prayogo Cahyo Prayogo Cahyo Prayogo Dhurrotul Roaidha Dimas Prastyaji Djuhari - Djuhari Djuhari Djuhari, Djuhari dwi nawang wulan eddrin wahyu febbiliani Ekachai Chukeatirote Endand Safitri, Endand erwin erwinn Feby Syaharani firman aprilianto dwi setiawan Firman Hidayat Hardadi Airlangga Hermawan Susanto hosinatul asror Indiyah Murwani Indiyah Murwani Indiyah Muwarni Indiyah Muwarni Khodiroh Shokibatun Mahayu Woro Lestari Maria Ulfah Miftahur Rohmah Mohammad Jasa Afroni nurhadiyanti rochmana Nurhidayati, Nurhidayati Nurlailah Nurlailah Putri Nur Azizah rosita dwi S Sugiarto S Sunawan sekar putri lisnanwati SITI MUSLIKAH Sugiarto Sugiarto Syafarotin Syafarotin Takaya Higuchi Tsuyoshi Imai Tsuyoshi Imai Tsuyoshi Imai Tsuyoshi Imai, Tsuyoshi wandi wandi Wiwit Nur Hidayah Yulia Nuraini Yuliana Yuliana