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All Journal Biospecies JOURNAL OF COASTAL DEVELOPMENT Berkala Perikanan Terubuk Journal of Aquatropica Asia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan
Esti Harpeni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Other

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Journal : Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

 1 
Eksplorasi Bakteri yang Berasosiasi dengan Karang Lunak sebagai Alternatif Sumber Senyawa Bioaktif: Uji Hayati Antibakteri Esti Harpeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.283

Abstract

Soft coral has a significant function in the ecology of coral reef which produces secondary metabolites. One of very serious problems with coral reef ecosystem related to secondary metabolite improvement was coral supply because of need of many soft corals producing a little amount of secondary metabolites. The purposes of this research were to isolate and to select soft coral bacteria that have ability to produce antibacterial having been used to inhibit Escherichia coli and Staphylococcus aureus. The research was conducted from March 31th to November 2nd, 2005 by using description method. Sampling was carried out at Pulau Tegal Waters on two different coral genera, namely Briareum and Sinularia at three different sites. Laboratory studies were performed at Environment and Healthy Monitoring Fish Laboratory at Lampung Marine Agricultural. The results showed that five of 125 isolates obtained had potential of producing antibacterial that have been used to inhibit E.coli and S. aureus. The coral ability to inhibit E.coli and S. aureus was indicated by inhibition zone around soft coral bacteria colonies. Morphology and biochemistry characterization of potentially inhibiting isolates against E. coli and S. aureus resulted in strains Staphylococcus sp. (isolate code B1I10), Plesiomonas sp. (isolate code B1I11), Actinobacillus sp. (isolate code B2I6), Actinomyces sp. (isolate code B1II4), and    Aerococcus sp. (isolate code S1III2). 
Screening of Potential Probiotic Vibrio sp. Against Vibriosis in the Litopenaeus vannamei Munti Sarida; Esti Harpeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.197

Abstract

Vibriosis is a bacterial disease generated by Vibrio sp., particularly V. harveyi that becomes the biggest cause of up to 100% shrimp mortality. Using antibiotics as the curative step is not applicable anymore, because of the residual effects to the shrimp and environment and the resistant effects to the humans as well. The purpose of this study was to screen potentially probiotic from strain Vibrio sp. that could inhibit V. harveyi as an effort of handling vibriosis diseases. Water sample from the shrimp ponds were isolated and purified in SWC-agar and TCBS-agar and then were incubated overnight at the room temperature. Bacterial concentrations were calculated using MC Pharland method and spectrophotometer. In vivo test was conducted for the best candidate of potential probiotic isolate, i.e. CP1. Challenged test was leaded within 4 different bacterial concentrations as the treatments and 2 replications using 10 shrimps as the tested organisms per aquarium. The treatments were 107 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (1), 106 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (2), 105 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (3) and PBS (4) as the control.  Our study showed that the Survival Rate of the tested organisms was 90% in the treatment 1. This result indicated the capability of growth inhibition of the candidate probiotic bacterium against V. harveyi. Presumably, Vibrio sp. could be used in the vanamei culture as the probiotic. Finally, the candidate probiotic bacterium was identified as Vibrio furnissii.
Co-Authors Agus Setyawan Ahmad Mustawa Arlin Wijayanti Arthur Lemuel David Asep Saefulloh Assovaria . Berta Putri Eko EFFENDI Eva Susanti Hilma Putri Fidyandini Ignatius Sandra Setyabudi Juliana Marbun Limin Santoso Moh. Muhaemin Muhammad Ridho Taris Munti Sarida Nandya Dwinitasari Novi Santika Nurma Jana Hazzulli Rara Diantari Rico Wahyu Prabowo Rissa Amelia Septiarini - Sera Hardiyani Sri Waluyo Suliswati Suliswati Supono Supono Syohibahttul Islamiyah BAHAR Triga Royana Sagala Uun Febriyani Wardiyanto - Wardiyanto Wardiyanto Yudha Trinoegraha Adiputra