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All Journal Biospecies JOURNAL OF COASTAL DEVELOPMENT Berkala Perikanan Terubuk Journal of Aquatropica Asia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan

Esti Harpeni

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Author-ID : 450221

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Other

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Respon Imun Spesifik Larva Ikan Mas (Cyprinus carpio) melalui Imunitas Maternal yang Diberi Vaksin Inaktif Whole Cell Aeromonas salmonicida Syohibahttul Islamiyah BAHAR; Esti HARPENI; Eko EFFENDI
Biospecies Vol. 10 No. 1 (2017): Januari 2017
Publisher : Universitas Jambi

Aeromonas salmonicida are specific bacteria that can cause infections and death to the cultivation of carp (Cyprinus carpio) during larval stage. Death in carp can be prevented by a vaccine, but the vaccine can only be given on the seed over the age of 3 weeks. Maternal vaccination needs to be done to improve the immune system of the larvae by means of inactivated whole cell vaccine A. salmonicida on broodstock ready to spawn. Aims to determine the effectiveness of vaccines on breeders carp to the parent antibody titer test and larvae, as well Survival Rate (SR) and the Relative Percent Survival (RPS) larvae. This research was conducted with a completely randomized design, 4 treatments A (control); B (0.3 ml/kg); C (0.4 ml/kg); D (0.5 ml/kg) and 3 repetitions. The results show that the antibody titer of 0.3 ml dose capable of providing agglutination reaction to pitting 7th (64x dilution) in broodstock, and vaccine doses 0,4ml on broodstock able to give agglutination reaction to the larvae until all 6 wells (32x dilution). A dose of 0.4 ml/kg resulted the highest SR and RPS with 96.11% and 81.25% respectively. Clinical symptoms of redness in control larvae was spread throughout the body whereas on the vaccine treatment was only in certain body parts.Keywords: A. salmonicida, vaccines, maternal immunity, larva, specific immune respon

ANALISIS UJI TANTANG UDANG VANAME (Litopenaeus vannamei) YANG DIBERI BAKTERI PROBIOTIK Bacillus sp. D2.2 DAN EKSTRAK UBI JALAR SEBAGAI SINBIOTIK Arlin Wijayanti; Nandya Dwinitasari; Uun Febriyani; Esti Harpeni; Wardiyanto Wardiyanto
Biospecies Vol. 11 No. 2 (2018): Volume 11, No.2, July, 2018
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.723 KB)

In vaname shrimp cultivation system, bacterial disease is a problem that often arises that mainly caused by the bacteria Vibrio sp. Sinbiotic application is one way to prevent disruption of the disease. Sinbiotic is nutritional supplements that made from the combination of probiotic and prebiotic that can provide beneficial effects to the host. This research uses a local sinbiotic of probiotic bacteria Bacillus sp. D2.2, which comes from the traditional ponds in the Mulyosari village, Pasir Sakti district, Lampung Timur Regency, Lampung. Prebiotic that used in this research made from meal extracts of purple sweet potato that had the best capability to support the growth of bacteria probiotic Bacillus sp. D2.2. This research aims to know the effectivity of sinbiotic against the infection of vibriosis againts to vaname shrimp by doing analysis of clinical symptoms and tissue damage post test challenge using bacteria Vibrio harveyi. The results showed that addition sinbiotic gave effect to SR 6,25 %, MTD 13,80 hour, and clinical symptoms and then tissue damage is lower than without the feeding of sinbiotik.

LIFE HISTORY STUDIES OF Montipora digitata IN PIONEER BAY, NORTH QUEENSLAND, AUSTRALIA Esti Harpeni; Arthur Lemuel David
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 1 (2011): Volume 15, Number 1, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (643.947 KB)

Intertidal zone in reef flat is the extreme condition for many species of corals. Periodical tides, wave action and sedimentation become the main challenging factors which usually avoided by most of coral species. However, Montipora digitata is the species that found abundant in these conditions. Having relatively small and short-lived colony, forced this species to find the strategy to survive and maintain the energy efficiently. Understanding biology and life history of M. digitata in the coral reefs is the key to understanding how this species can adapt to the extreme conditions. The biology and life history of this study focused on the population size structure, reproduction, competitive interactions, investment repair and maintain and types of selection. M. digitata leaded to productivity as a strategy to cope with any extreme condition. This pattern relatively matched with r-and S-R selection.

THE POTENTIAL ROLES OF INTERFERON IN MANAGING VIRAL DISEASES IN CRUSTACEAN Esti Harpeni
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 2 (2011): Volume 14, Number. 2, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.008 KB)

World crustacean aquaculture has developed considerably since the 1980’s and is now worth more than USD 10 billion per year. However, the economic growth of this industry has been severely affected by problems related to diseases and environmental degradation. Viral disease outbreaks are particularly concerning and have caused massive economic losses around the world. However, there is still no effective treatment for most viral diseases. Current research on crustacean diseases focuses on the role of innate immune system as the first defence mechanism against viral infections. Of the available antiviral immune responses, interferons (IFNs) are known to have ability interfering effect on viral replication, particularly in vertebrates. This paper reviews the function and molecules involved in the vertebrate interferon system and whether similar molecules and pathways may exist in crustacean immune systems. Therefore, IFN or IFN-like proteins in crustaceans may provide the key to managing viral diseases.

EFFECT BIOFLOC SYSTEM AND PROBIOTICS Bacillus sp. D2.2 THAT NON SPECIFIC IMMUNE RESPONSE OF TILAPIA Oreochromis niloticus (Linn, 1758) INFECTED BY Aeromonas hydrophila Triga Royana Sagala; Supono Supono; Esti Harpeni
Berkala Perikanan Terubuk Vol 48, No 1 (2020): Februari 2020
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Disease in tilapia is caused by Aeromonas hydrophila. The disease has an effect on reduction of production cultivation and economic losses. The control efforts taken are to improve the non specific immune response in tilapia cultivated with biofloc system and probiotics against bacterial infection of Aeromonas hydrophila. This study aims to determine effect the non specific immune response in tilapia cultivated with biofloc system and probiotics against bacterial infection of Aeromonas hydrophila infection. The design used in this study completely randomized design with four treatments, control, biofloc, probiotics in feed and a combination of feed probiotics and biofloc. The parameters observed included total leukocytes, differential leukocyte, survival rate, relative percent survival, and water quality. The results showed that cultivated tilapia with bioflok, probiotics, and a combination of both provides a significant immune response to the parameters of total leukocytes and survival rate compared to control and immune response increases after injected bacteria Aeromonas hydrophila.

UTILIZATION OF SAMBUNG NYAWA LEAF EXTRACTS Gynura procumbens (Lour) Merr. FOR TREATMENT OF Vibrio alginolyticus IN TIGER GROUPER (Epinephelus fuscoguttatus Forsskal, 1775) Novi Santika; Wardiyanto Wardiyanto; Esti Harpeni
Berkala Perikanan Terubuk Vol 47, No 2 (2019): Juli 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Tiger Grouper is one of the sea water fish commodities that is quite popular with the community and has a high economic value. The problem faced by farmers is the attack of Vibriosis, one of which is caused by the Vibrio alginolyticus bacteria. The use of synthetic antibiotics has been widely used but has many adverse effects, so it needs new alternatives for the treatment of Vibriosis disease. One of them is by using the extract of lifelong leaf extract. Life-sustaining plants (Gynura procumbens) contain secondary metabolites such as flavonoids, tannins, and antibacterial saponins. This study aims to determine the best dosage of lifelong leaf extract for the treatment of Vibriosis disease in tiger grouper. The study was conducted in two stages, namely in vitro and in vivo. Before the fish were treated with feed that had been given a sambung deca leaf extract, the fish were challenged using Vibrio alginolyticus with a density of 108 CFU / mL as much as 0.1 mL / head and then fed with treatment and maintained for 21 days. The results of the in vitro study showed that the life of sambung leaf extract at a dose of 700 ppm had a broad inhibitory effect on V. alginolyticus, which amounted to 10.47 mm compared to other treatments. Whereas when continued for in vivo testing, a dose of 350 ppm in general has been applied for the treatment of attacks of Vibrio alginolyticus in tiger grouper.

PENGGUNAAN EKSTRAK DAUN JAMBU BIJI (Psidium guajava Linnaeus) SEBAGAI IMUNOSTIMULAN IKAN MAS (Cyprinus carpio L) YANG DIINFEKSI MOTILE AEROMONAS SEPTICEMIA Rissa Amelia; Esti Harpeni; Hilma Putri Fidyandini
Journal of Aquatropica Asia Vol 6 No 2 (2021): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/aquatropica.v6i2.2614

This study aims to analyze the effect of the various concentration of guava leaf extract (Psidium guajava Linnaeus) on the prevention of Motile Aeromonas Septicemia disease in common carp (Cyprinus carpio Linnaeus). In a total of 150 common carp, length 9-11 cm with a density of 10 carps/aquarium. This study used a completely randomized design through 5 treatments, each treatment has 3 replications, namely (K+) consists of without guava Psidium guajava leaf extracts and without bacteria-infection Aeromonas hydrophila, (K-) consists of without guava Psidium guajava leaf extracts with bacteria-infection Aeromonas hydrophila, (A) the application of 125 ppm guava Psidium guajava leaf extracts, (B) the application of 250 ppm guava leaf extracts, (C) the application of 500 ppm guava Psidium guajava leaf extracts for 23 days. The parameters observed total of leukocytes, differensial leukocytes, hematocrit levels, erythrocyte, hemoglobin, phagocytic activity (AF), phagocytic index (IF), relative percent survival (RPS), survival rate (SR) and water quality. Data were analyzed with Anova 95% confidence interval and continued with Duncan test. The parameter also showed a significant result (P<0,05) with the application of guava Psidium guajava leaf extracts to the total leukocytes, differensial leukocytes, erythrocyte, hemoglobin, phagocytic activity, phagocytic index and relative percent survival. The results of this research indicate the concentration of 250 ppm guava Psidium guajava leaf extracts is the optimum application to increase common carp non-spesific immune responses.

IMPROVEMENT IN THE GROWTH PERFORMANCE OF TIGER GROUPER Epinephelus fuscoguttatus (Forsskal, 1775) BY PROBIOTIC MICROCAPSULES, Bacillus sp. D2.2 Ignatius Sandra Setyabudi; Esti Harpeni; Wardiyanto Wardiyanto
AQUASAINS Vol 8, No 2 (2020)
Publisher : Jurusan Perikanan dan Kelautan

Probiotics in the fish's body are able to optimize the nutrition of feed given for growth. Microcapsule techniques can protect probiotics as they pass through the digestive tract. This study aims to study the increase in growth of tiger grouper fish fed with probiotic Bacillus sp. Microcapsules. D2.2 with different dosages. The study was conducted in May - June (30 days) with 5 treatments and 3 replications. The treatments applied are K- (feed without probiotics), K + (feed + liquid probiotics), A (feed + probiotic microcapsules 1 gram / kg of feed), B (feed + probiotic microcapsules 2 gram / kg of feed), C (feed + probiotic microcapsules 3 grams / kg of feed). The following are the highest observations, namely absolute weight gain (23.8 ± 0.7) grams, specific growth rate (1.35 ± 0.04)% /day, feed covertion ratio (FCR) (1.3 ± 0.1) ), survival rate (83 ± 2.9)% and protein digestibility 90.56% while the lowest value is in the control treatment. The results showed that the administration of probiotic microcapsules was able to significantly increase the growth of tiger grouper fish compared to control treatments.

Penerapan Manajemen Kesehatan Panti Benih Udang Di Kalianda Lampung Selatan Rico Wahyu Prabowo; Sri Waluyo; Yudha Trinoegraha Adiputra; Rara Diantari; Esti Harpeni
AQUASAINS Vol 3, No 2 (2015)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.762 KB)

Shrimp culture drives the national economy that are included in the program of industrialization of fisheries in the economic development programs of blue ( blue economy ). Shrimp farming in Lampung province remains a mainstay of the region as a foreign exchange earner . Kalianda , South Lampung is the center hatchery tiger shrimp (Penaeus monodon) and vannamei shrimp (Litopeneaeus vannamei ) the largest in Lampung. research conducted to observe the shrimp health management applied by growers of black tiger shrimp and vannamei shrimp in South Lampung and learn relationship between seed health management with quality seeds and tiger prawn shrimp vannamei shrimp health management dihasilkan. Data obtained by interview and the quality of shrimp seed obtained from observations in the laboratory with the observed light response, swimming activity, abnormality, gut contents, lipid droplets, the condition of the hepatopancreas and pigmentation. There are 22 hatchery were observed with the application of the value of health management in the high category for the third hatchery , hatchery medium category 6 and category bneih low at 13 homes. The quality of virus-free shrimp WSSV and IHHNV and the amount of bacteria in the body are normal and healthy shrimp was observed in the overall nursing bneih diamati. Relation between hatchery management application health and seed quality is reflected by the equation Y = 0,019X2 - 1,358X + 52.49 with the coefficient of determination ( R2 ) of 0.419. This relationship indicates that the quality of shrimp seed is determined by the health of hatchery management practices (r = 0.647). This study reinforces the importance of implementing health management hatchery known by farmers with biosecurity as a form of sustainable shrimp farming .

ASSAY OF Acinetobacter AS COMPETITOR TO VIBRIOSIS LUMINESCENCE BACTERIA IN PACIFIC PACIFIC WHITE SHRIMP(Litopenaeus vannamei) Assovaria .; Esti Harpeni; Asep Saefulloh
AQUASAINS Vol 4, No 1 (2015)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.196 KB)

Pacific white shrimpculture is having problems caused by bacteria infection. The cause of bacteria infection is V. harveyi, it can decrease seed production. Pathogenicity of vibriosis can decrease with inhibition by other types of bacteria. The aim of the research was to get competitor V.harveyi isolates through identification and challenge test in vitro and in vivo. The result of screening of bacterial isolates were obtained from cultivation supplement products to produced 4 pure isolates with different morphologically colonies and cells. There was one potential isolate could inhibits V. harveyi by inhibition test in vitro. Biochemical test from three different laboratories were identified that Acinetobacter sp. as competitor to V. harveyi and it was not pathogenic to Pacific white shrimp. Challenge test Acinetobactersp. against V. harveyi on Pacific white shrimp larvae was conducted through three treatments, without given V.harveyi and Acinetobacter sp. As controls; treatment with given bacteria V.harveyi and treatment with given V. harveyi and Acinetobacter sp. The challenge test results showed that treatment with given of V.harveyi and Acinetobacter sp. may decreased V. harveyi density of 6.3x106CFU/mL to 7.5x103CFU/mL which could suspected to decrease pathogenicity of competition number of colonies.

Co-Authors Agus Setyawan Ahmad Mustawa Arlin Wijayanti Arthur Lemuel David Asep Saefulloh Assovaria . Berta Putri Eko EFFENDI Eva Susanti Hilma Putri Fidyandini Ignatius Sandra Setyabudi Juliana Marbun Limin Santoso Moh. Muhaemin Muhammad Ridho Taris Munti Sarida Nandya Dwinitasari Novi Santika Nurma Jana Hazzulli Rara Diantari Rico Wahyu Prabowo Rissa Amelia Septiarini - Sera Hardiyani Sri Waluyo Suliswati Suliswati Supono Supono Syohibahttul Islamiyah BAHAR Triga Royana Sagala Uun Febriyani Wardiyanto - Wardiyanto Wardiyanto Yudha Trinoegraha Adiputra

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