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Journal : Biospecies

Respon Imun Spesifik Larva Ikan Mas (Cyprinus carpio) melalui Imunitas Maternal yang Diberi Vaksin Inaktif Whole Cell Aeromonas salmonicida Syohibahttul Islamiyah BAHAR; Esti HARPENI; Eko EFFENDI
Biospecies Vol. 10 No. 1 (2017): Januari 2017
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.797 KB) | DOI: 10.22437/biospecies.v10i1.3486

Abstract

Aeromonas salmonicida are specific bacteria that can cause infections and death to the cultivation of carp (Cyprinus carpio) during larval stage. Death in carp can be prevented by a vaccine, but the vaccine can only be given on the seed over the age of 3 weeks. Maternal vaccination needs to be done to improve the immune system of the larvae by means of inactivated whole cell vaccine A. salmonicida on broodstock ready to spawn. Aims to determine the effectiveness of vaccines on breeders carp to the parent antibody titer test and larvae, as well Survival Rate (SR) and the Relative Percent Survival (RPS) larvae. This research was conducted with a completely randomized design, 4 treatments A (control); B (0.3 ml/kg); C (0.4 ml/kg); D (0.5 ml/kg) and 3 repetitions. The results show that the antibody titer of 0.3 ml dose capable of providing agglutination reaction to pitting 7th (64x dilution) in broodstock, and vaccine doses 0,4ml on broodstock able to give agglutination reaction to the larvae until all 6 wells (32x dilution). A dose of 0.4 ml/kg resulted the highest SR and RPS with 96.11% and 81.25% respectively. Clinical symptoms of redness in control larvae was spread throughout the body whereas on the vaccine treatment was only in certain body parts.Keywords: A. salmonicida, vaccines, maternal immunity, larva, specific immune responĀ 
ANALISIS UJI TANTANG UDANG VANAME (Litopenaeus vannamei) YANG DIBERI BAKTERI PROBIOTIK Bacillus sp. D2.2 DAN EKSTRAK UBI JALAR SEBAGAI SINBIOTIK Arlin Wijayanti; Nandya Dwinitasari; Uun Febriyani; Esti Harpeni; Wardiyanto Wardiyanto
Biospecies Vol. 11 No. 2 (2018): Volume 11, No.2, July, 2018
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.723 KB)

Abstract

In vaname shrimp cultivation system, bacterial disease is a problem that often arises that mainly caused by the bacteria Vibrio sp. Sinbiotic application is one way to prevent disruption of the disease. Sinbiotic is nutritional supplements that made from the combination of probiotic and prebiotic that can provide beneficial effects to the host. This research uses a local sinbiotic of probiotic bacteria Bacillus sp. D2.2, which comes from the traditional ponds in the Mulyosari village, Pasir Sakti district, Lampung Timur Regency, Lampung. Prebiotic that used in this research made from meal extracts of purple sweet potato that had the best capability to support the growth of bacteria probiotic Bacillus sp. D2.2. This research aims to know the effectivity of sinbiotic against the infection of vibriosis againts to vaname shrimp by doing analysis of clinical symptoms and tissue damage post test challenge using bacteria Vibrio harveyi. The results showed that addition sinbiotic gave effect to SR 6,25 %, MTD 13,80 hour, and clinical symptoms and then tissue damage is lower than without the feeding of sinbiotik.