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All Journal Indonesian Journal of Cancer Chemoprevention The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Jurnal EduHealth
Ivan Arie Wahyudi
Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Public Health Veterinary

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Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ Nurdiana Dewi; Ahmad Syaify; Ivan Arie Wahyudi
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 3 (2013): September 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.845 KB) | DOI: 10.20473/j.djmkg.v46.i3.p135-139

Abstract

Background: Amelogenin is a major protein constituent of the developing enamel matrix that is critical for enamel formation. Mutations of amelogenin cause hypoplastic enamel phenotypes. Previous research found that infant of diabetic mother has higher risk for having enamel hypoplasia. Purpose: The aim of this study was to determine the effect of gestational diabetes mellitus on the expression of amelogenin in Wistar rats offspring tooth germ. Methods: Sixteen female Wistar rats, aged 2.5-3 months, body weight 150-200 g were used in this study, Wistar rats were mated and divided into two groups and treated on day 0 of pregnancy. Group A was DM group, consisting of 8 rats, induced by streptozotocin (STZ) injection 40 mg/kg BW. Group B was control group, consisting of 8 rats received citrate buffer injection. Thirty-two rat pups were decapitated on day 5. Immunohistochemical procedures were performed on molar tooth germ of the mandibular rat pups using antibody anti-AMELX to determine the expression of amelogenin. Examination carried out on the images using ImageJ software. All data were then statistically analyzed by Mann Whitney test. Results: There was no significant difference in the expression of amelogenin in the DM group and control group (p>0.05). Conclusion: Gestational diabetes mellitus did not affect the expression of amelogenin in rat offspring tooth germ. Further study is needed to examine the pattern of amelogenin expression with measurement of glucose levels of rat pups.Latar belakang: Amelogenin merupakan protein terbanyak pada matriks email yang berperan penting dalam pembentukan email. Mutasi pada amelogenin dapat menyebabkan email menjadi hipoplastik. Penelitian sebelumnya menunjukkan bahwa anak yang dilahirkan oleh ibu pengidap diabetes memiliki resiko lebih tinggi untuk mengalami hipoplasia email. Tujuan: Penelitian ini bertujuan untuk meneliti pengaruh diabetes mellitus gestasional terhadap ekspresi amelogenin pada benih gigi anak tikus Wistar. Metode: Enam belas ekor tikus Wistar betina, umur 2,5-3 bulan, berat badan 150-200 g digunakan dalam penelitian ini, dikawinkan kemudian dibagi menjadi dua kelompok dan diberi perlakuan pada kehamilan hari ke-0. Kelompok A merupakan kelompok diabetes mellitus, terdiri atas 8 ekor tikus diberi perlakuan diabetes mellitus dengan injeksi streptozotocin (STZ) 40 mg/kg BB. Kelompok B merupakan kelompok kontrol, terdiri atas 8 ekor tikus diberi injeksi buffer sitrat. Tiga puluh dua anak tikus yang lahir didekapitasi pada hari ke- 5. Dilakukan prosedur imunohistokimia pada benih gigi molar rahang bawah anak tikus menggunakan antibodi anti-AMELX untuk mengetahui ekspresi amelogenin. Pemeriksaan dilakukan pada hasil foto menggunakan software ImageJ. Hasil kemudian dianalisa menggunakan uji Mann Whitney. Hasil: Tidak terdapat perbedaan yang bemakna pada ekspresi amelogenin kelompok kontrol dan kelompok diabetes mellitus (p>0,05). Simpulan: Diabetes mellitus gestasional tidak mempengaruhi ekspresi amelogenin pada benih gigi anak tikus. Diperlukan penelitian lebih lanjut untuk mengetahui pola ekspresi amelogenin dengan pengukuran kadar glukosa darah anak tikus.
Effect of Citrus aurantifolia swingle essential oils on methyl mercaptan production of Porphyromonas gingivalis Anindya Prima Yusinta; Ivan Arie Wahyudi; Anne Handrini Dewi
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 1 (2013): March 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.288 KB) | DOI: 10.20473/j.djmkg.v46.i1.p50-54

Abstract

Background: Halitosis is a term used to describe an unpleasant odors emanating timely from oral cavity. The unpleasant smell of breath most common caused from volatile sulphure compound (VSC). Methyl mercaptan is the major component of VSC. P. gingivalis produced large amount of methyl mercaptan. The essential oils of Citrus aurantifolia swingle contain antibacterial component. Purpose: The purpose of this study was to determine the effect of essential oil of Citrus aurantifolia swingle on the production of methyl mercaptan compounds in P. gingivalis. Methods: Bacterial suspension of P. gingivalis in TSB medium with 108 CFU/ml concentration cultured in a microplate and added by the essential oils of Citrus aurantifolia swingle with 1%, 2%, 3% and 4% concentration. Distilled water was used as negative control and 0.2% Chlorhexidine mouthwash was used as a positive control. Microplate was incubated anaerobically for 48 hours. After the periode of incubation, 0.6% methionine as the exogenous substrate and 0.06% DTNB as a reagen for determining methyl mercaptan concentration were added to each wells. The microplate was futher incubated for 12 hours. Concentration of methyl mercaptan produced by the P. gingivalis was measured spectrophotometrically using microplate reader at 415 nm. Results: One-way ANOVA showed that the essential oil of Citrus aurantifolia swingle take effect on the concentration of methyl mercaptan produced by P. gingivalis. LSD test results indicated that there was a significant difference of methyl mercaptan concentration between treatment groups of the essential oils of Citrus aurantifolia swingle and distilled water that used as negative control. Conclusion: The essential oil of Citrus aurantifolia swingle has decreased the production of methyl mercaptan produced by P. gingivalis.Latar belakang: Halitosis adalah istilah yang digunakan untuk menggambarkan bau tidak sedap yang berasal dari rongga mulut. Penyebab utama halitosis adalah senyawa volatile sulphur compound (VSC) dan metil merkaptan merupakan komponen VSC yang paling dominan menyebabkan halitosis. P. gingivalis dapat memproduksi metil merkaptan dalam jumlah banyak. Minyak atsiri kulit jeruk nipis (Citrus aurantifolia swingle) memiliki kandungan antibakteri di dalamnya. Tujuan: Untuk mengetahui pengaruh minyak atsiri kulit jeruk nipis terhadap produksi senyawa metil merkaptan pada bakteri P. gingivalis. Metode: Suspensi bakteri P. gingivalis dalam media TSB dengan konsentrasi 108 CFU/ml dibiakkan dalam microplate. Selanjutnya dilakukan penambahan minyak atsiri kulit jeruk nipis konsentrasi 1%, 2%, 3%, dan 4%. Obat kumur chlorhexidine 0,2% digunakan sebagai kontrol positif dan akuades sebagai kontrol negatif. Microplate diinkubasi selama 48 jam untuk selanjutnya dilakukan penambahan metionin 0,6% dan DTNB 0,06% dan diinkubasi kembali selama 12 jam. Konsentrasi senyawa metil merkaptan yang diproduksi oleh bakteri P. gingivalis dihitung dengan menggunakan microplate reader pada panjang gelombang 415 nm. Hasil: ANOVA satu jalur menunjukkan bahwa minyak atsiri kulit jeruk nipis berpengaruh terhadap konsentrasi metil merkaptan yang diproduksi oleh bakteri P. gingivalis. Hasil uji LSD menunjukkan adanya perbedaan yang signifikan antara kelompok perlakuan minyak atsiri kulit jeruk nipis dengan akuades sebagai kontrol negatif. Kesimpulan: Minyak atsiri kulit jeruk nipis dapat menurunkan produksi senyawa metil merkaptan yang dihasilkan oleh bakteri penyebab halitosis P. gingivalis.
Inhibition effect of cashew stem bark extract (Anacardium Occidentale L.) on biofilm formation of Streptococcus sanguinis Rizni Amaliah; Sri larnani; Ivan Arie Wahyudi
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 4 (2012): December 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.276 KB) | DOI: 10.20473/j.djmkg.v45.i4.p212-216

Abstract

Background: Biofilm is communities of microorganisms attached to solid surface and enclosed in extracellular matrix that protected microorganisms from antibacterial agents and host defense. One of bacteria might have a role in initial colonization of biofilm formation is Streptococcus sanguinis (S. sanguinis). Previous studies showed that cashew stem bark extract (Anacardium occidentale L.) can inhibit the growth of Streptococcus strains. Purpose: The purpose of this study was to determine the inhibition effect of cashew (Anacardium occidentale L.) stem bark ethanol extract on biofilm formation of S. sanguinis. Methods: Streptococcus sanguinis grown in Brain Heart Infusion (BHI) + 2% sucrose medium by using microplate polystyrene 96 wells. The samples were divided into 3 groups, 5% polyethyleneglycol (PEG) as negative control, cashew stem bark extract (concentration 3.125 mg/ml, 6.25 mg/ml, 9.375 mg/ml, and 12.5 mg/ml), and 0.12% chlorhexidine (as positive control). Biofilm was stained by 1% crystal violet. Afterwards, optical density (OD) of samples were measured by microplate reader λ 595 nm. The data of biofilm formation inhibition percentage were analyzed by one way ANOVA and then continued by Least Significant Difference (LSD) test. Results: The result of one way ANOVA showed that there were significant differences in inhibition of S. sanguinis biofilm formation (p<0.05). LSD test showed that concentration extract 3.125 mg/ml had significant difference with concentration 9.375 mg/ml and 12.5 mg/ml. Reciprocally, concentration 6.25 mg/ml had significant difference with concentration 9.375 mg/ml and 12.5 mg/ml. Conclusion: Cashew stem bark extract was able to inhibit biofilm formation of S. sanguinis.Latar belakang: Biofilm merupakan sekumpulan mikroorganisme yang melekat pada permukaan solid dan diselubungi oleh matriks ekstraseluler yang melindungi mikroorganisme dari bahan-bahan antibakteri dan sel-sel pertahanan tubuh. Salah satu bakteri yang berperan pada awal pembentukan biofilm adalah Streptococcus sanguinis (S. sanguinis). Beberapa penelitian menunjukkan bahwa ekstrak kulit batang jambu mete (Anacardium occidentale L.) dapat menghambat pertumbuhan bakteri strain Streptococcus. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol kulit batang jambu mete (Anacardium occidentale L.) terhadap pembentukan biofilm S. sanguinis. Metode: Media pertumbuhan S. sanguinis menggunakan Brain Heart Infusion (BHI) + 2% sukrosa yang ditumbuhkan pada microplate polystyrene 96 wells. Kelompok perlakuan dibagi menjadi tiga kelompok yaitu PEG 5% (kontrol negatif), ekstrak kulit batang jambu mete (konsentrasi 3,125 mg/ml, 6,25 mg/ml, 9,375 mg/ml, dan 12,5 mg/ml), dan klorheksidin 0,12% (kontrol positif). Biofilm yang terbentuk diwarnai dengan crystal violet 1%. Kemudian optical density (OD) sampel diukur menggunakan microplate reader λ 595 nm. Data berupa persentase penghambatan pembentukan biofilm dianalisis menggunakan uji one way ANOVA dan dilanjutkan dengan uji Least Significant Difference (LSD). Hasil: Uji one way ANOVA menunjukkan terdapat perbedaan daya hambat pembentukan biofilm S. sanguinis yang signifikan (p<0,05). Hasil uji LSD menunjukkan konsentrasi 3,125 mg/ml memiliki perbedaan yang signifikan dengan konsentrasi 9,375 mg/ml dan konsentrasi 12,5 mg/ml. Begitu juga dengan konsentrasi 6,25 mg/ml memiliki perbedaan yang signifikan dengan konsentrasi 9,375 mg/ml dan konsentrasi 12,5 mg/ml. Kesimpulan: Ekstrak kulit batang jambu mete dapat menghambat pembentukan biofilm S. sanguinis.
Analgesic, Anti-Inflammatory and Anti-Biofilm-Forming Activity of Potato (Solanum tuberosum L.) Peel Extract Ivan Arie Wahyudi; Fahri Reza Ramadhan; Rama Insan Kusuma Wijaya; Retno Ardhani; Trianna Wahyu Utami
Indonesian Journal of Cancer Chemoprevention Vol 11, No 1 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss1pp30-35

Abstract

The utilization of natural resources, one of which is plants, has been researched as an alternative to synthetic drugs because of their natural content. Potato (Solanum tuberosum L.) peels, the parts of potatoes that are often cut off and discarded, have been reported to have some phenolic compounds and flavonoids in their composition. The extract of potato peels was investigated for its analgesic, anti-inflammatory, and anti-biofilm-forming properties. A hot plate test was conducted to assess the analgesic activity in treatment doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg with paracetamol as the reference drug and distilled water as the negative control, while carrageenan-induced paw edema was used to assess anti-inflammatory activity in treatment doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg with diclofenac as the reference drug and distilled water as the negative control. Anti-biofilm-forming activity was tested by using the crystal violet assay. The results showed that, compared with the negative control, treatment doses of 100 mg/kg and 200 mg/kg significantly (p < 0.05) reduced pain stimuli, whereas a treatment dose of 100 mg/kg, 200 mg/kg, and 400 mg/kg significantly (p < 0.05) reduced the edema volume increment. However, compared with the positive control, paracetamol and diclofenac were associated with the least pain stimulus and the least edema volume increment, respectively. Potato peel extract against S. mutans biofilm formation demonstrated effectiveness (p < 0.05). Based on these data, it can be concluded that potato peel extract has analgesic, anti-inflammatory, and anti-biofilm-forming activities, as demonstrated in this study.
The Relationship Between Oral Health Knowledge And Oral Health Behavior Among Students Of Sman 1 Kerinci Kanan Arunda Prastio; Ivan Arie Wahyudi; Prayudha Benni Setiawan
Jurnal EduHealth Vol. 14 No. 03 (2023): Jurnal EduHealt, Edition September 2023
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54209/jurnaleduhealth.v14i3.2450

Abstract

Based on basic health research in 2018, the percentage of dental and oral problems in Indonesia is relatively high, namely 57.6%. One of the groups is prone to dental and oral health problems in adolescents. Knowledge and behavior influence and play an important role in dental and oral health directly. The purpose of this study was to determine the relationship between oral health knowledge and oral health maintenance behaviors in students aged 15 – 18 at SMAN 1 Kerinci Kanan, Siak Regency, Riau Province. The cross-sectional study was conducted on 192 students in grades 10 – 12 using a random sampling method. The research instrument used questionnaires regarding oral health knowledge and behaviors that have been tested for validity and reliability. The analysis used to see the relationship between oral health knowledge variables and oral health behavioral behaviors in the study subjects was carried out using the Spearman Rank correlation technique (p < 0.05). The results of the study obtained 74% of students had good oral health knowledge and 59.9% had sufficient oral health behaviors. The results of the Spearman Rank test obtained that a p-value of 0.017 which (p < 0.05) and the Spearman correlation coefficient showed a positive value of 0.172. Conclusion: there is a significant relationship between oral health knowledge variables and oral health behaviors in students of SMAN 1 Kerinci Kanan, Siak Regency, Riau Province. The better the oral knowledge possessed, the better the oral health maintenance
Co-Authors Ahmad Syaify, Ahmad Anindya Prima Yusinta Anne Handrini Dewi Archadian Nuryanti Arunda Prastio Dewi Nurdiana Fahri Reza Ramadhan Fatma Yuza Fatma Yuza, Fatma Hiroko Hagita Keiko Miyoshi Like Rosita Dwi Susanto Like Rosita Dwi Susanto, Like Malida Magista, Malida Merry Angel, Merry Nuning Wahyu Utami Nunuk Purwanti, Nunuk Prayudha Benni Setiawan Rama Insan Kusuma Wijaya Retno Ardhani Rizni Amaliah Sri Larnani Sri larnani Sri Larnani Sri Larnani, Sri Taigo Horiguchi Takafumi Noma Taro Muto Trianna Wahyu Utami Zenia Adindaputri U, Zenia Adindaputri