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Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto, Rudiyanto; Efendi, Darda; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.

Comparison of the Reduction Effect of Sucrose and Table Sugar Concentration on Growth Characteristics of Red Ginger (Zingiber officinale Rocs.) Cultured in Liquid Medium Hapsari, Betalini Widhi; Ermayanti, Tri Muji; Rantau, Deritha E.; Rudiyanto, Rudiyanto
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/34

The aim of this research was to compare the reduction effect of sucrose or table sugar concentration on growth characteristics of red ginger cultured in MS liquid medium. Shoots of red ginger was cultured on MS liquid medium without addition of plant growth regulators, supplemented with 5, 10, and 20 g/l of sucrose or table sugar for 8 weeks. Resulted plantlets were acclimatized in a greenhouse to investigate their growth and survival rate. Numbers of stomata, chlorophyll concentration as well as cross section of leaves from plantlets grown in vitro were compared to those of transplants grown in the greenhouse. The results showed that the use of table sugar at concentration of 20 g/l gave the best growth of red ginger. Meanwhile, the reduction of table sugar from 20 to 10 g/l reduced growth and survival rate of in vitro shoots as well as that of transplants in the greenhouse. Only few shoots formed roots when they were grown on the medium containing 5 g/l of table sugar, and transplants failed to grow in the greenhouse. It found that the chlorophyll content of in vitro plantlets was lower than those of transplants grown in the glasshouse. However, the number of stomata of the in vitro plantlets was higher than that of transplants grown in the glasshouse. There was no anatomical abnormalities found on the cross section of leaves between in vitro plantlets and transplants grown in the greenhouse. The replacement of sucrose with table sugar may reduce the production cost of plantlets.Keywords: red ginger (Zingiber officinale Rocs.), reduction of sugar, survival rate, chlorophyll, stomata.

Pengaruh Modifikasi KH2PO4, NH4NO3 dan Sukrosa terhadap Pertumbuhan Tunas serta Pembentukan Umbi Mikro Taka (Tacca leontopetaloides) secara In vitro Rudiyanto, Rudiyanto; Hapsari, Betalini Widhi; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3658

ABSTRACTPolynesian arrowroot (Tacca leontopetaloides (L.) Kuntze), which is one of the bulbous herbaceous plants,have high nutritional value. Modification of macro nutrients by reducing nitrogen content and increasingphosphorus on the medium gave affects on shoot growth and initiated micro tuber formation on in vitrocultures. The aim of this research was to determine the effect of modified macro nutrients in combination withthe increase in sucrose concentrations on shoot growth and micro tuber formation of T. leontopetaloides. Theexperimental design was factorial completely randomized design.The factors tested were modifications of MSmacro nutrients that were. M1 (170 mg/l KH2PO4 and 1650 mg/l NH4NO3; normal, control treatment); M2 (340mg/l KH2PO4 and 825 mg/l NH4NO3); and M3 (680 mg/l KH2PO4 and 412.5 mg/l NH4NO3 in combinationwith 30 (S1) (control treatment), 40 (S2), 50 (S3) and 60 g/l of sucrose (S4). The variables tested were shootheight, number of leaves, number of roots and number of micro tuber which were observed weekly at 0-8weeks after culturing. The results showed that the modification of macro nutrient in combination with sucroseconcentration had significant effect on shoot height, number of leaves and number of roots but not significanton the number of tubers. The highest shoots were found in M1S3 treatment, the highest number of leaves wasin M1S1 and M1S3 treatment and the highest number of roots was in M1S4 treatment. The number of tubersnot significantly different between the treatments tested.Keywords: in vitro, KH2PO4, microtuber, NH4NO3, sucrose, Tacca leontopetaloides

PENGARUH MODIFIKASI KH2PO4, NH4NO3 DAN SUKROSA TERHADAP PERTUMBUHAN TUNAS SERTA PEMBENTUKAN UMBI MIKRO TAKA (TACCA LEONTOPETALOIDES) SECARA IN VITRO Rudiyanto, Rudiyanto; Hapsari, Betalini Widhi; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3658

ABSTRACTPolynesian arrowroot (Tacca leontopetaloides (L.) Kuntze), which is one of the bulbous herbaceous plants,have high nutritional value. Modification of macro nutrients by reducing nitrogen content and increasingphosphorus on the medium gave affects on shoot growth and initiated micro tuber formation on in vitrocultures. The aim of this research was to determine the effect of modified macro nutrients in combination withthe increase in sucrose concentrations on shoot growth and micro tuber formation of T. leontopetaloides. Theexperimental design was factorial completely randomized design.The factors tested were modifications of MSmacro nutrients that were. M1 (170 mg/l KH2PO4 and 1650 mg/l NH4NO3; normal, control treatment); M2 (340mg/l KH2PO4 and 825 mg/l NH4NO3); and M3 (680 mg/l KH2PO4 and 412.5 mg/l NH4NO3 in combinationwith 30 (S1) (control treatment), 40 (S2), 50 (S3) and 60 g/l of sucrose (S4). The variables tested were shootheight, number of leaves, number of roots and number of micro tuber which were observed weekly at 0-8weeks after culturing. The results showed that the modification of macro nutrient in combination with sucroseconcentration had significant effect on shoot height, number of leaves and number of roots but not significanton the number of tubers. The highest shoots were found in M1S3 treatment, the highest number of leaves wasin M1S1 and M1S3 treatment and the highest number of roots was in M1S4 treatment. The number of tubersnot significantly different between the treatments tested.Keywords: in vitro, KH2PO4, microtuber, NH4NO3, sucrose, Tacca leontopetaloides

DETEKSI VARIASI SOMAKLONAL PLANLET Jatropha curcas Linn. HASIL REGENERASI EMBRIO SOMATIK DENGAN MARKA MOLEKULAR ISSR . Rudiyanto; Darda Efendi; Erwin Al-Hafiizh; Tri Muji Ermayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Physic nut (Jatropha curcas Linn.) has the potential as a source of sustainable biofuels. Somatic embryo proliferation of J. curcas may cause somaclonal variations. This research aimed to investigate somaclonal variations of J. curcas somatic embryo derived-plantlet using ISSR markers. Somatic embryos of J. curcas at the globular phase were cultured on liquid MS medium supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg L-1 of 2,4-D. Parameter observed were embryos weight, embryos volume, colour, and size of embryos. After proliferation, the embryos were cultured on a germination medium until the cotyledonary phase. The results showed that proliferation of J. curcas somatic embryos was optimal, with the highest weight and volume, at MS medium added with 1 mg L-1 2,4-D.

PERTUMBUHAN DAN MORFOLOGI KULTUR TUNAS SEMPUR (Dillenia philippinensis Rolfe) PADA MEDIA MS-BAP-NAA (Growth and Morphology of Sempur (Dillenia philippinensis Rolfe) Shoot Culture on MS –BAP-NAA Media) Deritha Ellfy Rantau; Dyah Retno Wulandari; Tri Muji Ermayanti; Rudiyanto Rudiyanto; Betalini Widhi Hapsari; Aida Wulansari; Evan Maulana; Herlambang Laksmana Firdaus
Jurnal Penelitian Hutan Tanaman Vol 18, No 1 (2021): Jurnal Penelitian Hutan Tanaman
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpht.2021.18.1.65-78

ABSTRACT Propagation of sempur (Dillenia philippinensis Rolfe) using conventional vegetative methods is considered ineffective due to slow process and low germination level. Propagation by tissue culture is considered more effective because it does not depend on the season and requires less plant material. It can benefit sempur conservation since it is categorized as threatened with extinction in 2020 on the IUCN red list. This study aimed to evaluate the effect of BAP and NAA on the growth of sempur’s shoots and to observe the morphology of shoot culture. The media used as a control was MS without growth regulators. The treatment medium was MS with the addition of BAP and NAA. Shoots were used as explants. Shoot growth and plantlet morphology were observed eight weeks after planting. The results indicated that the combination of 1 mg/l BAP and 0.5 mg/l NAA resulted in the highest total number of leaves, nodes and adventitious shoots. The combination of BAP and NAA, each 1 mg/l, resulted in the highest fresh weight and callus formation. The combination of 1 and 2 mg/l BAP and 0.5-1 mg/l NAA could not form roots. In comparison, the combination of BAP and NAA in culture media could change the shape and size of the leaves. The survival rate of growth of plantlets derived from MS medium was 50% at 22 weeks after acclimatization.Keywords: morphology, shoot culture, sempur, BAP (Benzil Amino Purin), NAA (Naphthalene Acetic Acid) ABSTRAK Perbanyakan tanaman sempur (Dillenia philippinensis Rolfe) dengan metode konvensional secara vegetatif memerlukan waktu lama dan daya kecambah biji juga rendah. Perbanyakan dengan kultur jaringan dianggap lebih menguntungkan karena tidak tergantung musim, memerlukan sedikit bahan tanaman dan bermanfaat untuk konservasi sempur karena termasuk spesies yang hampir terancam punah tahun 2020 dalam daftar merah IUCN. Penelitian ini bertujuan untuk mengevaluasi pengaruh BAP dan NAA terhadap pertumbuhan tunas sempur serta mengamati morfologi kultur tunas. Media yang digunakan sebagai kontrol adalah MS yang tidak mengandung zat pengatur tumbuh. Media perlakuan adalah MS dengan penambahan BAP dan NAA. Tunas pucuk dipergunakan sebagai eksplan. Pertumbuhan tunas dan morfologi planlet diamati pada delapan MST. Hasil pengamatan menunjukkan bahwa kombinasi 1 mg/l BAP dan 0,5 mg/l NAA menghasilkan jumlah total daun, buku dan jumlah tunas adventif tertinggi. Kombinasi BAP dan NAA masing-masing 1 mg/l menghasilkan bobot basah dan pembentukan kalus tertinggi. Kombinasi antara BAP 1 dan 2 mg/l serta NAA 0,5-1 mg/l tidak mampu membentuk akar. Kombinasi BAP dan NAA pada media kultur mengubah bentukan dan ukuran daun. Daya tumbuh planlet yang berasal dari media MS adalah 50% pada 22 minggu setelah aklimatisasi. Kata kunci: morfologi, kultur tunas, sempur, BAP (Benzil Amino Purin), NAA (Naphthalene Acetic Acid)

Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto Rudiyanto; Darda Efendi; Tri Muji Ermayanti
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.

Comparison of the Reduction Effect of Sucrose and Table Sugar Concentration on Growth Characteristics of Red Ginger (Zingiber officinale Rocs.) Cultured in Liquid Medium Betalini Widhi Hapsari; Tri Muji Ermayanti; Deritha E. Rantau; Rudiyanto Rudiyanto
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The aim of this research was to compare the reduction effect of sucrose or table sugar concentration on growth characteristics of red ginger cultured in MS liquid medium. Shoots of red ginger was cultured on MS liquid medium without addition of plant growth regulators, supplemented with 5, 10, and 20 g/l of sucrose or table sugar for 8 weeks. Resulted plantlets were acclimatized in a greenhouse to investigate their growth and survival rate. Numbers of stomata, chlorophyll concentration as well as cross section of leaves from plantlets grown in vitro were compared to those of transplants grown in the greenhouse. The results showed that the use of table sugar at concentration of 20 g/l gave the best growth of red ginger. Meanwhile, the reduction of table sugar from 20 to 10 g/l reduced growth and survival rate of in vitro shoots as well as that of transplants in the greenhouse. Only few shoots formed roots when they were grown on the medium containing 5 g/l of table sugar, and transplants failed to grow in the greenhouse. It found that the chlorophyll content of in vitro plantlets was lower than those of transplants grown in the glasshouse. However, the number of stomata of the in vitro plantlets was higher than that of transplants grown in the glasshouse. There was no anatomical abnormalities found on the cross section of leaves between in vitro plantlets and transplants grown in the greenhouse. The replacement of sucrose with table sugar may reduce the production cost of plantlets.Keywords: red ginger (Zingiber officinale Rocs.), reduction of sugar, survival rate, chlorophyll, stomata.

The effect of sucrose concentrations and different types of tube cap on in vitro growth of Dahlia (Dahlia sp.) using vermiculite as substrate Rudiyanto Rudiyanto; Betalini Widhi Hapsari; Deritha Ellfy Rantau; Tri Muji Ermayanti
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.31537

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