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All Journal UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Dental Journal (Majalah Kedokteran Gigi) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JFIOnline Canrea Journal: Food Technology, Nutritions, and Culinary Journal
Mochammad Yuwono
Departemen Ilmu Kefarmasian, Fakultas Farmasi, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Chemistry Dentistry Economics, Econometrics & Finance Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Efek Perbandingan Surfaktan dan Kosurfaktan Terhadap Karakteristik dan Efisiensi Penjebakan Ovalbumin Dalam Mikroemulsi Hendradi, Esti; Yuwono, Mochammad
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 1 (2016)
Publisher : Indonesian Research Gateway

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Abstract

ABSTRACT: The study was conducted to investigate the effect of composition of surfactant (Span 80 and Tween 80) and cosurfactant (butanol) on characteristics and ovalbumin entrapment in the microemulsion water in oil (w/o).The composition of surfactant (Span 80 and Tween 80) and cosurfactant (butanol) used were 6:3, 7:3 and 8:3. Evaluation was conducted on the surface tension, viscosity, droplet size, pH and the efficiency of ovalbumin trapping in microemulsion system. Results were analyzed by statistic using one way analysis of variance (ANOVA) with 95% confidence level (α = 0,05). From the results it was concluded that the composition of surfactant and cosurfactant 7:3 resulted in the best microemulsion. Keywords: microemulsion, surfactant, cosurfactant, butanol, ovalbumin ABSTRAK : Penelitian ini dilakukan untuk mengetahui pengaruh komposisi surfaktan (Span 80 dan Tween 80) dan kosurfaktan (butanol) terhadap karakteristik dan penjebakan ovalbumin dalam mikroemulsi minyak dalamm air (w/o). Perbandingan surfaktan (Span 80 dan Tween 80) dan kosurfaktan (butanol) adalah 6:3, 7:3 dan 8:3. Evaluasi yang dilakukan meliputi tegangan permukaan, viskositas, ukuran droplet, pH dan efisiensi penjebakan ovalbumin dalam sistem mikroemulsi. Hasil dianalisis dengan statistik menggunakan ANOVA dengan tingkat kepercayaan 95% (α = 0,05). Dari hasil penelitian ini disimpulkan bahwa bahwa perbandingan surfaktan dan kosurfaktan sebesar 7:3 menghasilkan mikroemulsi yang terbaik. Kata Kunci: mikroemulsi, surfaktan, kosurfaktan, butanol, ovalbumin    
Efek Perbandingan Surfaktan dan Kosurfaktan Terhadap Karakteristik dan Efisiensi Penjebakan Ovalbumin Dalam Mikroemulsi Hendradi, Esti; Yuwono, Mochammad
Jurnal Farmasi Indonesia Vol 8, No 1 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.779 KB) | DOI: 10.35617/jfi.v8i1.203

Abstract

ABSTRACT: The study was conducted to investigate the effect of composition of surfactant (Span 80 and Tween 80) and cosurfactant (butanol) on characteristics and ovalbumin entrapment in the microemulsion water in oil (w/o).The composition of surfactant (Span 80 and Tween 80) and cosurfactant (butanol) used were 6:3, 7:3 and 8:3. Evaluation was conducted on the surface tension, viscosity, droplet size, pH and the efficiency of ovalbumin trapping in microemulsion system. Results were analyzed by statistic using one way analysis of variance (ANOVA) with 95% confidence level (α = 0,05). From the results it was concluded that the composition of surfactant and cosurfactant 7:3 resulted in the best microemulsion. Keywords: microemulsion, surfactant, cosurfactant, butanol, ovalbumin ABSTRAK : Penelitian ini dilakukan untuk mengetahui pengaruh komposisi surfaktan (Span 80 dan Tween 80) dan kosurfaktan (butanol) terhadap karakteristik dan penjebakan ovalbumin dalam mikroemulsi minyak dalamm air (w/o). Perbandingan surfaktan (Span 80 dan Tween 80) dan kosurfaktan (butanol) adalah 6:3, 7:3 dan 8:3. Evaluasi yang dilakukan meliputi tegangan permukaan, viskositas, ukuran droplet, pH dan efisiensi penjebakan ovalbumin dalam sistem mikroemulsi. Hasil dianalisis dengan statistik menggunakan ANOVA dengan tingkat kepercayaan 95% (α = 0,05). Dari hasil penelitian ini disimpulkan bahwa bahwa perbandingan surfaktan dan kosurfaktan sebesar 7:3 menghasilkan mikroemulsi yang terbaik. Kata Kunci: mikroemulsi, surfaktan, kosurfaktan, butanol, ovalbumin    
INFLUENCE OF OLEIC ACID ON THE IN VITRO PENETRATION OF DICLOFENAC SODIUM GEL Lidya Ameliana; Esti Hendradi; Mochammad Yuwono
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Diclofenac sodium (DS) is a nonsteroidalantiinflammatory drugs (NSAIDs), that inhibitscyclooxygenase-2 enzyme (COX-2). It has first passmetabolism by 40-50%, because of its shortbiological half-life, the drug has to be givenfrequently (Ganiswara, 2005). Dose of DS in gel is 1%(Sweetman, 2007). In peroral use, it may cause therisk of gastrointestinal bleeding, cardiovascular,hypersensitivity reactions and disorders of thecentral nervous (Katzung, 2002, Chuasuwan, et al.,2008).DS does not penetrate well through skin and cannotreach the effective concentration at the site ofaction after transdermal application (Mohammed,2001; Ozguney, et al., 2006). DS partition coefficientin n-octanol-buffer aqua (log P) was 1.1 (Chuasuwan,2008). Lipophilic nature of the stratum corneum andthe hydrophilic nature of the underlying tissuesshowed that the drug will penetrate the skin shouldhave an optimal balance between the lipophilic andhydrophilic properties. In general, the skin is morepermeable to the material which has a partitioncoefficient (P) in octanol-water between 10-1000(Michniac-Kohn et al., 2005).Oleic acid enhances the penetration of drugs intothe skin by increasing the fluidity of the stratumcorneum lipids through the establishment ofchannels (water channels) (Fang , et al , 2003)This study aimed to know the influence of OleicAcid (OA) in several concentrations (1%, 3% and5%) as penetration enhancer on the in vitropenetration of topical DS gel. Furthermore, thephysical properties of the topical preparation(organoleptic, pH and viscosity), and DS penetrationpercutaneously through rat skin . Then observedthat in spite of DS assay and penetrate through therat skin at certain intervals and observed by meansof HPLC.
Optimasi Metode KCKT untuk Penetapan Kadar 4-Isobutilasetofenon dan 2-(4-Isobutirilfenil) Asam Propanoat dalam Tablet Ibuprofen Rara Dyah Chrissanti; Asri Darmawati; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 7 No. 1 (2020): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v7i12020.26-34

Abstract

Pendahuluan: Ibuprofen (IBP) banyak digunakan sebagai obat anti piretik dan pereda nyeri, obat ini sensitif terhadap oksidasi dan suhu. Cemaran IBP yang keberadaannya dalam tablet harus memenuhi syarat uji batas adalah 4-isobutilasetofenon (IBP RC C) dan 2-(4-isobutirilfenil) asam propanoat (IBP RC J). Variasi matriks tablet seringkali membuat prosedur standar untuk analisis cemaran perlu dioptimasi karena dapat menyebabkan kinerja kromatografi yang tidak memenuhi syarat kompendia. Tujuan: Penelitian ini bertujuan untuk memperoleh kolom dan kondisi operasional KCKT yang selektif, efisien dan memenuhi persyaratan kompendia untuk pengujian IBP dan cemarannya. Metode: Penelitian ini dirancang untuk membandingkan kinerja kromatografi (resolusi, faktor ikutan dan jumlah lempeng teoritis) beberapa kolom KCKT C18 yang sesuai persyaratan United States Pharmacopoeia (USP) untuk penetapan cemaran organik dalam tablet ibuprofen. Instrumen KCKT yang digunakan adalah Shimadzu Prominence dengan detektor Photo Diode Array (PDA). Kondisi analisis optimum yang diperoleh diaplikasikan untuk analisis 3 macam sediaan tablet IBP. Hasil: Kondisi optimum diperoleh melalui kolom C18-C 4,6 x 250 mm, 5 µm,  menggunakan fase gerak larutan asam kloroasetat 1% pH 3,0 : asetonitril (45:55, v/v), laju alir 1,5 mL/menit, suhu kolom ambient, detektor PDA      254 nm, volume injeksi 10 µL dengan waktu analisis 11 menit. Diperoleh puncak-puncak yang simetris dengan kinerja kromatografi yang memenuhi persyaratan USP. Kesimpulan: Metode yang telah dioptimasi berhasil diaplikasikan pada 3 sediaan tablet yang beredar di pasaran dan dapat digunakan untuk pengujian rutin dalam rangka pengawasan kualitas produk obat tablet ibuprofen.
Optimasi Metode KCKT-ELSD dengan Pemisahan HILIC untuk Penetapan Kadar Glukosamin Hidroklorida pada Suplemen Kesehatan Etik Wahyuningsih; Riesta Primaharinastiti; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 7 No. 2 (2020): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v7i22020.80-87

Abstract

Pendahuluan: Analisis glukosamin hidroklorida dalam suplemen kesehatan memiliki beberapa kesulitan dikarenakan rendahnya absorpsi pada daerah UV dan sifat kepolarannya yang tinggi. Tujuan: Mendapatkan kondisi optimum metode kromatografi cair kinerja tinggi dengan detektor hamburan cahaya evaporatif (KCKT-ELSD) menggunakan kolom kromatografi cair interaksi hidrofilik (HILIC) untuk pemisahan glukosamin hidroklorida dalam suplemen kesehatan. Metode: Optimasi kondisi KCKT dilakukan terhadap pengaruh konsentrasi amonium format, pH fase gerak, dan komposisi asetonitril dalam fase gerak pada pemisahan glukosamin hidroklorida dalam suplemen makanan. ZIC-HILIC 150 x 4,6 mm, 5 μm digunakan sebagai kolom. Optimasi kondisi ELSD dilakukan terhadap pengaruh suhu nebulasi dan evaporasi. Hasil: Pemisahan terbaik yang menghasilkan puncak simetris dari glukosamin hidroklorida dicapai dalam kondisi isokratik menggunakan komposisi fase gerak asetonitril : air : 30 mM ammonium format (77 : 3 : 20, v/v/v) pada pH 4,5, suhu kolom 35°C, laju alir fase gerak 1 mL/menit, dan volume injeksi sampel 5 μL. Suhu nebulisasi dan evaporasi berturut-turut adalah 50°C dan 80°C, dengan laju aliran nitrogen adalah 1,10 standar liter per menit (SLM). Kesimpulan: Kondisi optimum KCKT-ELSD dengan mode HILIC yang diperoleh dalam penelitian ini menghasilkan kromatogram dengan puncak glukosamin hidroklorida yang simetris, dan terpisah secara selektif dan sensitif dari komponen matriks suplemen kesehatan.
Optimasi dan Validasi Metode KCKT untuk Identifikasi dan Penetapan Kadar Metabolit Nitrofuran dalam Bakso Udang Rachma Ayuningtyas; Riesta Primaharinastiti; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 8 No. 2 (2021): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v8i22021.117-124

Abstract

Pendahuluan: Dikarenakan sifat dari Furazolidon, furaltadon, dan nitrofurantoin yang karsinogenik, perlu dilakukan pengawasan terhadap cemaran metabolit nitrofuran dalam produk berbahan dasar udang. Nitrofuran dimetabolisme secara cepat dalam jaringan tubuh hewan. Tujuan: Penelitian ini bertujuan melakukan validasi metode KCKT untuk penetapan kadar metabolit nitrofuran dalam bakso udang. Metode: Metabolit furazolidone, furaltadone dan nitrofurantoin yaitu 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), dan 1-aminohydantoin (AHD) diderivatisasi menggunakan 2-nitrobenzaldehida, diekstraksi dalam etil asetat dan dicuci dengan n-heksana, kemudian disuntikkan ke dalam system KCKT dengan detektor UV-PDA. Pemisahan dilakukan dengan kolom C-18 46 x 250 mm, 5 mm. Sebagai fase gerak adalah ammonium asetat 20 mM : asetonitril (70:30), dengan laju alir 0.5 mL/menit, suhu kolom 40oC, dan volume injeksi 100 µL. Hasil: Metode yang digunakan dapat memisahkan semua metabolit dari senyawa pengotor dengan waktu analisis 40 menit, dan menghasilkan linieritas yang baik dalam rentang konsentrasi 51,90 – 103,79 ng/mL (AOZ); 56,63 - 101,39 ng/mL (AMOZ); dan 49,92 - 89,86 ng/mL (AHD). Akurasi metode dipresentasikan sebagai rekoveri dengan hasil sebesar 78,50 - 102,29% untuk AOZ; 77,02 - 99,87% untuk AMOZ; and 85,54 - 99,77% untuk AHD. Presisi metode dinyatakan dalam simpangan baku relatif (relative standard deviation, RSD) dengan hasil masing-masing adalah 6,10 - 19,90%; 3,68 - 17,75%; and 2,75 - 12,58% untuk AOZ, AMOZ, dan AHD. LOD dan LOQ sebesar 3,09 ng/mL dan 10,29 ng/mL (AOZ); 6,84 ng/mL dan 22,82 ng/mL (AMOZ); serta 4,61 ng/mL dan 15,36 ng/mL (AHD). Kesimpulan: Metode uji dapat digunakan untuk skrining awal untuk mendeteksi dan penetapan kadar cemaran metabolit nitrofuran pada bakso udang.
HPLC Method Optimization for Simultaneous Determination of Quercetin, Luteolin, Sinensetin, and Stigmasterol in Herbal Medicines Asih Imulda Hari Purwani; Riesta Primaharinastiti; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 9 No. 1 (2022): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v9i12022.62-68

Abstract

Background: Quercetin, luteolin, sinensetin and stigmasterol each is the main marker compound in extracts of Sonchus arvensis, Plantago major, Orthosiphon stamineus, and Strobilanthes crispus, respectively. These extracts show nephrolithiasis activity. For quality control of herbal medicines, a high performance liquid chromatography (HPLC) method has been developed in this study using quercetin, luteolin, sinensetin and stigmasterol as phytochemical markers. Objective: to show optimal conditions of analysis and evaluate the stability of quercetin, luteolin, sinensetin and stigmasterol. Methods: The optimal conditions for analysis were carried out by determining the composition of the mobile phase, the flow rate, and the detector's wavelength. Zorbax Eclipse Plus C18 150 x 4.6 mm, 5 μm was used as the column. The stability test was done by analyzing the standard and samples stored at 4oC for 0, 3, 6 and 24 hours. Results: The best separation of the extract was achieved under isocratic conditions using a mixture of water: methanol: phosphoric acid: acetic acid : acetonitrile (50: 30: 0.05: 0.05: 20 v/v/v/ v/v) as mobile phase with detector wavelength of 352 nm, a mobile phase flow rate of 1 mL/min, and a sample injection volume of 10 μL. Conclusion: In this study, the optimal condition for analysis of quercetin, luteolin, sinensetin and stigmasterol. Quercetin, luteolin, sinensetin and stigmasterol were not stable during 6 hours storage, therefore, standard solutions and samples should be made fresh to maintain the stability.
Determination of fluoride in black, green and herbal teas by ionselective electrode using a standard-addition method Mochammad Yuwono
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 2 (2005): June 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.133 KB) | DOI: 10.20473/j.djmkg.v38.i2.p91-95

Abstract

Tea leaves are very rich in fluoride, since tea plants take up fluoride from the soil and accumulate in its leaves. Some of this fluoride is released into the infusion, which is drunk as tea. Fluoride in tea could be beneficial for the prevention of dental caries, but it may result in excessive intake and lead to enamel fluorosis. The purpose of this work was to determine the fluoride levels in 12 different brands and types of tea by means of a computer-controlled ion-selective electrode potentiometry using a standard-addition method. It is a rapid method which showed good accuracy and precision. Fluoride contents of tea infusions after 5 min ranged from 0.95 to 4.73 mg/l for black teas; from 0.70 to 1.00 mg/l for green teas, and from 0.26 to 0.27 mg/l for herbal teas. It was concluded that black teas and green teas examined may be important contributors to the total daily fluoride intake. However, the ingestion of some black teas that were found to have high fluoride content by children at the age of risk to dental fluorosis should be avoided.
Effect of chlorophyll in alginate-based edible film in inhibiting spoilage of fish snacks Eko Nurcahya Dewi; Asmi Citra Malina A.R. Tassakka; Mochammad Yuwono; Eko Agus Suyono; Lukita Purnamayati; Jamaluddin Fitrah Alam
jurnal1 VOLUME 5 ISSUE 1, JUNE 2022
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v5i1.571

Abstract

Edible films are environmentally biodegradable materials used for food packaging. The edible green alga Caulerpa racemosa has antimicrobial properties; however, its chlorophyll-based bioactive compounds can be damaged when heated so it is prepared in microcapsules. Our research evaluated the effect of Caulerpa microcapsules on an alginate-based edible film on film properties and food spoilage. The microcapsules were used at concentrations of 0%, 0.5%, 1%, and 1.5%. The edible film was measured for film properties, total phenolic content (TPC), antioxidant activity (DPPH), functional group (FTIR) and microstructure (SEM). Food spoilage was evaluated on a popular fish-based product (fish bubble snacks). Bacterial strains of Escherichia coli and Staphylococcus aureus were used to evaluate antimicrobial effectiveness of the edible films. The addition of Caulerpa microcapsules had no significant negative effect on physical properties of the alginate-based film, while the smoother and more homogenous surface should enhance the barrier properties of the film. The slow and evenly distributed release of active compounds from the microcapsules increased resistance to Rhizopus sp. and significantly reduced the proliferation of E. coli but not S. aureus on coated fish snacks. Caulerpa racemosa can be used to enhance the effectiveness of alginate-based films in delaying spoilage and could extend product shelf-life
Optimization Method and Stability Test to Determinate Luteolin, Quercetin, Apigenin, and Sinensetin Levels in Herbal Medicines Using TLC-Densitometry Muhammad Hidayatullah; Mochammad Yuwono; Riesta Primaharinastiti
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 9 No. 3 (2022): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v9i32022.235-241

Abstract

Background: Nephrolithiasis is a condition in which there are one or more kidney stones in the pelvis or calyces. Luteolin, quercetin, apigenin, and sinensetin are marker compounds in the extracts of Plantago major, Sonchus arvensis, Strobilanthes crispus and Orthosiphon stamineus which have nephrolithiasis activity. To control the quality of herbal medicines, a TLC-Densitometry method was developed in this study using luteolin, quercetin, apigenin, and sinensetin as phytochemical markers. Objective: The present work aimed to develop optimal conditions for analyzing luteolin, quercetin, apigenin, and sinensetin. Methods: Determination of optimal conditions for analysis is carried out by determining the composition of the mobile phase, chamber saturation time, and analysis wavelength. Silica gel 60 F254 was used as the stationary phase. Stability tests were carried out by analyzing standards and samples at 0, 4, 8, and 24 hours. Results: The best separation that produces symmetrical peaks of herbal medicine was achieved under isocratic conditions using the composition of the mobile phase chloroform : acetone: dichloromethane : acetonitrile : formic acid (6 : 2: 2 : 0,05 : 0.05 v/v/v/v/ v) with a wavelength of 335 nm with a saturation time of 30 minutes. Conclusion: In this study, the optimal conditions for the analysis of luteolin, quercetin, apigenin, and sinensetin. Luteolin, quercetin, apigenin, and sinensetin are unstable during 8 hours of storage. Therefore, standard solutions and samples must be made fresh to maintain stability.
Co-Authors Albertus Aditya Setiawan Ameliana, Lidya Angkasa Megistra Ulvan Asih Imulda Hari Purwani Asmi Citra Malina Asri Darmawati Bastiana Bermawi Eko Agus Suyono Eko Nurcahya Dewi Esti Hendradi Etik Wahyuningsih Farida Ifadotunnikmah Fawaz Shihab Jamaluddin Fitrah Alam Julian Dwi Susanto Juni Ekowati Laili Irfanah Lukita Purnamayati Muhammad Hidyatullah Neny Prawitasari Rachma Ayuningtyas Rara Dyah Chrissanti Riesta Primaharinastiti Ryan Afandi Suzana Thalia Vanessa Hartono Winanda Rizki Febrianti