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All Journal Biomedical Journal of Indonesia SRIWIJAYA JOURNAL OF MEDICINE
Lusia Hayati, Lusia
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Identifikasi Mutasi Gen rpob Ser531Leu Mycobacterium tuberculosis Yang Berhubungan Dengan Resistensi Rifampisin Amalia, Ella; Nindatama, Maghfiroh Rahayu; Hayati, Lusia; Handayani, Dwi
Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya Vol 1, No 1 (2015)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Tuberkulosis (TB) adalah penyakit infeksi yang disebabkan oleh Mycobacterium tuberculosis. Kasus resistensi M. tuberculosis terhadap rifampisin sudah banyak dilaporkan di dunia termasuk Indonesia. Resistensi terhadap rifampisin pada  M. tuberculosis sebagian besar disebabkan mutasi gen rpoB yang menyandi RNA polymerase subunit ß. Mutasi gen rpoB Ser531Leu yang berhubungan dengan resistensi terhadap Rifampisin paling sering terjadi. Adanya mutasi pada rpoB akan  menyebabkan perubahan pada struktur dan aktivitas target obat. Penelitian ini bertujuan untuk mengidentifikasi mutasi gen rpoB Ser531Leu Mycobacterium tuberculosis pada sampel yang diambil dari penderita tuberkulosis paru di RSUP dr. Mohammad Hoesin Palembang. Penelitian ini merupakan penelitian deskriptif terhadap 40 penderita tuberkulosis paru di RSUP dr. Mohammad Hoesin Palembang. Identifikasi mutasi gen rpoB Ser531Leu Mycobacterium tuberculosis dilakukan dengan teknik Multiplex Polymerase Chain Reaction menggunakan primer rpoB531. Dari 40 isolat gen rpoB kodon 531 didapatkan 70% (21 dari 30) terjadi mutasi, wild type sebanyak 9 isolat (30%) dan isolat yang tidak menghasilkan pita sebanyak 10 isolat. Telah ditemukan mutasi gen rpoB Ser531Leu Mycobacterium tuberculosis pada penderita tuberkulosis paru di RSUP dr. Mohammad Hoesin Palembang.
Prevalence of CYP2C19 Gene Polymorphism and Its Influence In Omeprazole Metabolism As Predictors Of Drug Inoxification In Malay Ethnic In South Sumatra: Prevalensi Polimorfisme Gen CYP2C19 dan Pengaruhnya Dalam Metabolisme Omeprazole Sebagai Prediktor Intoksikasi Obat Pada Etnis Melayu Di Sumatera Selatan Triwani, Triwani; Hayati, Lusia
Sriwijaya Journal of Medicine Vol. 1 No. 2 (2018): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Cytochrome P450 2C19 (CYP2C19) is an enzyme complex that plays a role in the metabolism of several drugs and is part of the super family of cytochrome P450. Genetic polymorphisms in these enzymes are associated with the emergence of poor metabolic phenotypes (poor metabolizers / PMs and intermediate metabolizers / IMs) that have a poor ability to metabolize the drugs that become substrates. Genotypes and phenotypes were analyzed using PCR-RFLP and bio-analysis of omeprazole levels in 30 subjects from ethnic Malay living in South Sumatra. Markers used to assess the presence of polymorphisms in the CYP2C19 gene are two polymorphic sites of exon 5 (CYP2C19 * 2) and exon 4 (CYP2C19 * 3). 321 bp DNA bands for exon 5 and 271 bp for exon 4 will be produced after DNA amplification by PCR method under denaturation for 5 min at 95oC; followed by 60 seconds at 95oC, 60sec at 53oC and 60sec at 72oC for 30 cycles; as well as the final polymerization for 5 minutes at 72 ° C. Furthermore, DNA cutting was done using the restriction enzyme endonuclease SmaI (CYP2C19 * 2) at 30oC and BamHI (CYP2C19 * 3) with incubation at 37oC for 3 hours. Bioanalysis of omeprazole levels in the blood with LC-MS. The results of this study indicate the presence of polymorphisms on both sites will eliminate the enzyme sites SmaI and BamHI. The results showed that 46.7% of South Sumatran Malay populations were classified as PM consisting of 13.3% homozygous mutandan mutant 33.4% heterozygotes. The high phenotype of PM enzyme CYP2C19 in ethnic Malays in South Sumatra predicted to influence metabolism of drugs become substrates. However, based on spearman correlation analysis, the correlation value was 0.035 with p = 0.875. This means that between the CYP2C19 gene polymorphism and the omeprazole levels in the blood there is a weak and meaningless correlation. The results of this study provide an overview of the high genetic polymorphisms of dyspepsia syndrome patients from the Malay population, ie almost half of the study subjects (46.7%). There is a weak and insignificant correlation between polymorphism and omeprazole levels.
Hubungan Polimorfisme Gen p53 Codon 72 dengan Kejadian Kanker Serviks di Rumah Sakit Dr. Mohammad Hoesin Palembang Hayati, Lusia; Delvia, Siska
Sriwijaya Journal of Medicine Vol. 1 No. 3 (2018): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Di Indonesia angka kejadian kanker serviks diperkirakan sekitar 50 per 100.000 penduduk. Diperkirakan ada lebih dari 1 juta perempuan di seluruh dunia dengan kanker serviks dan sebagian besar dari mereka belum didiagnosis atau tidak memiliki akses untuk skrining dan perawatan. Polimorfisme pada gen p53 codon 72 dapat mempengaruhi risiko kanker serviks melalui regulasi proliferasi dan apoptosis sel. Tujuan untuk mengetahui hubungan polimorfisme gen p53 codon 72 dengan kejadian kanker serviks. Penelitian ini merupakan penelitian observasional analitik. Pelaksanaan penelitian dengan pemeriksaan di laboratorium Biologi Molekuler Fakultas Kedokteran Universitas Sriwijaya dengan pendekatan studi Kasus Kontrol (Case-Control). Terdapat 70 sampel yang dibagi 2 kelompok, yaitu 35 Kasus dan 35 Kontrol. Penentuan Genotip dan Alotip menggunakan teknik PCR-RFLP. Hasil penelitian menunjukkan ada perbedaan signifikan pada karakteristik responden yang diamati antar kelompok kasus dan kontrol. Hasil genotip gen p53 codon 72 pada kelompok kasus terdapat 7 subjek (20,0%) yang memiliki genotip Pro/Pro dan Arg/Arg 5 subjek (14,3%) dan ada 23 subjek (65,7%) yang memiliki memiliki genotip Pro/Arg. Pada kelompok kontrol terdapat 28 subjek (80,0%) yang memiliki genotip Pro/Pro dan Arg/Arg 0 subjek (0,0%) dan ada 7 subjek (20,0%) yang memiliki memiliki genotip Pro/Arg. Frekuensi alel Prolin pada kelompok kasus 37 alel (52,9%) dan alel Arginin 33 alel (47,1%) sedangkan pada kelompok kontrol alel Prolin 63 (90%) dan alel Arginin 7 alel (10%). Hasil uji chi square genotip didapatkan p value 0.000, OR 1,304 and CI 95% 1,071-5,891, sedangkan pada alel didapatkan ρ value 0,000; OR 8,027 ; CI 95% 3,228 - 19,962.Disimpulkan ada hubungan antara genotip dan alel polimorfisme gen p53 codon 72 dengan kejadian kanker serviks.
CORRELATION BETWEEN SERUM FERRITIN LEVELS AND NUTRITIONAL STATUS OF THALASSEMIA-BETTA MAJOR PATIENTS Rafika, Rafika; Marwoto, Djoko; Hayati, Lusia
Biomedical Journal of Indonesia Vol 5, No 2 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i2.7986

Abstract

Thalassemia is a chronic disease that requires repeated blood transfusions, due to impaired hemoglobin synthesis due to mutations of one or more globin genes. Continuous transfusion can cause iron accumulation in the body, especially the heart, liver and endocrine organs, and can cause stunted growth and malnutrition or poor nutrition. This study aims to determine the correlation between serum ferritin levels and the nutritional status of thalassemia-betta major patients in RSUP Dr. Moh. Hoesin Palembang. This research is an observational analytic study with cross sectional design. The sample of this study was thalassemia-betta major patient who was hospitalized in the Department of Pediatrics. Mohammad Hoesin from October to November 2016 who met the participation criteria. Of the 43 patients, the majority (60.5%) had lean bodies according to their weight index according to age (BW / U) and were short stature according to their height index according to age (TB / U), but only 14.0% of patients had undernourished nutrition BB / TB index. Obtained average values of serum ferritin 2837.69 g / L, with a range of 278.7-13867 g / L. The results of the correlation test between serum ferritin levels and nutritional status indicate p = 0.326 and r = 0.153. There was no significant correlation between serum ferritin levels and the nutritional status of thalassemia-betta major patients.
Regulation of Cell Cycle Rachmat Hidayat; Hayati, Lusia
Biomedical Journal of Indonesia Vol. 6 No. 3 (2020): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i3.236

Abstract

The cell cycle is a vital process in the life of every organism. Normally, the cell cycleresults in cell division. Cell division consists of 2 main processes, namely DNAreplication and chromosome division that has been duplicated into 2 daughter cells.Cyclin is expressed periodically so that the cyclin concentration varies at each phase ofthe cell cycle. Cdks are threonine or serine protein kinases which must bind to cyclinfor its activation. The concentration of Cdks is relatively constant during the cell cycle.Cyclin-dependent kinase inhibitor (CKI) is a protein that can inhibit Cdk activity bybinding to Cdk or the cyclin-Cdk complex
Fertilization, Cleavage and Implation Hayati, Lusia
Sriwijaya Journal of Medicine Vol. 3 No. 3 (2020): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v3i3.229

Abstract

Most of the livestock in Indonesia are still conventional or traditional farms, where the quality of the seeds, the use oftechnology and the skills of farmers are still relatively low, thus affecting the productivity and genetic quality oflivestock. Therefore it is necessary to have knowledge about livestock reproduction that discusses fertilization,cleveage and implantation. Fertilization or fertilization (singami) is the fusion of two gametes which can be a nucleusor nucleus cells to form a single cell (zygote) or fusion of the nucleus. the process starts with the preparation of ovumcells and spermatozoa; penetration; core incorporation; and early zygote cleavage. Fertilization phase is the meetingbetween sperm cells and ovum cells and will produce zygote. Zygote will perform cell division (cleavage). The zygotethen undergoes growth and development through stages, namely division, gastrulation, and organogenesis.Implantation or also known as oxidation is the process of implanting the embryo, which is the result of conception,into the uterine wall (endometrium) to further develop.
Co-Authors Dwi Handayani Ella Amalia, Ella Maghfiroh Rahayu Nindatama, Maghfiroh Rahayu Marwoto, Djoko RACHMAT HIDAYAT Rafika Rafika Siska Delvia Triwani Triwani