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All Journal Abdimas Sainteknol : Jurnal Sains dan Teknologi Unnes Science Education Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Pembelajaran Biologi : Kajian Biologi dan Pembelajarannya Life Science
Lutfia Nur Hadiyanti, Lutfia Nur
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Semarang State University, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Social Sciences

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Journal : Biosaintifika: Journal of Biology

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Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA Fibriana, Fidia; Hadiyanti, Lutfia Nur
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6748

Abstract

In this study, twenty local durian accessions obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generated from the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed variations for the different accessions, whereas polymerase chain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restriction analyses with the restriction endonucleases Bsp1431yielded a restriction pattern for each accessions. The results of this study can be utilized by local durian farmers as a preliminary reference for durian propagation. The data obtained need to be supported by further research using the other molecular markers to obtain more accurate data. The clear identity of durian species can help the management of propagation systems by farmers to get superior local durian.How to CiteFibriana, F., & Hadiyanti, L. N. (2016). Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA. Biosaintifika: Journal of Biology & Biology Education, 8(3), 362-370.
Co-Authors Aji Purwinarko, Aji Andreas Priyono Budi Prasetyo Ari Widodo Fidia Fibriana Melati, Inaya Sari Nana Kariada Trimartuti Nur Kusuma Dewi Solichin Solichin Sri Sukaesih