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All Journal Abdimas Sainteknol : Jurnal Sains dan Teknologi Unnes Science Education Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Pembelajaran Biologi : Kajian Biologi dan Pembelajarannya Life Science
Lutfia Nur Hadiyanti, Lutfia Nur
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Semarang State University, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Social Sciences

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Journal : Sainteknol : Jurnal Sains dan Teknologi

 1 
PHYLOGENETIC RELATIONSHIPS OF LOCAL DURIAN SPECIES BASED ON MORPHOLOGICAL CHARACTERISTICS AND PCR-RFLP ANALYSIS OF THE RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) DNA Fibriana, Fidia; Hadiyanti, Lutfia Nur
Sainteknol : Jurnal Sains dan Teknologi Vol 14, No 2 (2016): December 2016
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v14i2.8990

Abstract

In this study, twentydurian species obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generatedfrom the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed a variation for the differentspecies, whereas polymerasechain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restrictionanalyses with the restriction endonucleases Bsp1431yielded a restriction pattern for eachspecies.
Co-Authors Aji Purwinarko, Aji Andreas Priyono Budi Prasetyo Ari Widodo Fidia Fibriana Melati, Inaya Sari Nana Kariada Trimartuti Nur Kusuma Dewi Solichin Solichin Sri Sukaesih