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All Journal Jurnal Bios Logos Protobiont: Jurnal Elektronik Biologi BERITA BIOLOGI Jurnal Biologi Tropis IJIS - Indonesian Journal On Information System JURNAL PENGABDI Biosaintifika: Journal of Biology & Biology Education Agroprimatech Jurnal Borneo Akcaya : Jurnal Penelitian dan Pengembangan Pelayanan Publik Seminar Nasional Peningkatan Mutu Pendidikan Life Science
Rikhsan Kurniatuhadi, Rikhsan
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Tanjungpura, Pontianak, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil Rousdy, Diah Wulandari; Rahmawati, Rahmawati; Kurniatuhadi, Rikhsan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7499

Abstract

Peat soil is a type of soil that dominates the island of Borneo. Typical compounds in peat soil is humic acid. Various in vitro studies performed have shown peat subtropical humic compounds can stimulate the immune system. However, in vivo study on animal has not been done. This study aimed to determine the effect of humic acid extracted from peat soil of Borneo against the immune system, both of non-specific and specific immunity Wistar rats (Rattus novergicus). Research using a completely randomized design with five treatments and five replicates, the normal controls, a positive control (isoprinosine), humic acid 125; 250; 500 mg/kg. Humic acid was administered orally for 10 days. The results showed humic acid adduction did not significantly affect levels of hemoglobin, erythrocytes and hematocrit. Humic acid adduction of 125 mg/kg significantly affects the total leukocyte count and differential leukocyte. Humic acid 125 mg/kg also showed increased phagocytic index better than normal controls. All humic acid treatments do not provide a significant effect on the total amount of antibody. The results of this study can be used for the development of Borneo tropical peat resources as natural imunostimulant.How to CiteRousdy, D. W., Rahmawati, R. & Kurniatuhadi, R. (2016). Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil. Biosaintifika: Journal of Biology & Biology Education, 8(3), 401-406.
MIKROANATOMI LIMPA TIKUS PUTIH (Rattus novergicus L.)SETELAH PEMBERIAN ASAM HUMAT DARI TANAH GAMBUT KALIMANTAN Wulandari Rousdy, Diah; Rahmawati; Kurniatuhadi, Rikhsan; Kurniadi, Edi
JURNAL BORNEO AKCAYA Vol 4 No 1 (2017): Jurnal Penelitian dan Pengembangan Pelayanan Publik
Publisher : Badan Penelitian dan Pengembangan Provinsi Kalimantan Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51266/borneoakcaya.v4i1.80

Abstract

Peat soil is organic soil types that has various biological potential to be developed. Humic acid compounds in the peat soil have a variety of potential biological one as immunostimulant. The aimed of this study to determine the effect of humic acid extracted from peat soil spleen histology Kalimantan against white male rats (Rattus novergicus L.) strain Wistar. Peat soil samples were taken in Pontianak on sapris maturity level. Humic acid is separated from other humic substances using IHSS methode. This research was done by completely randomized design with 4 treatments ie positive control (isoprinosin 380,3 mg/kg), humic acid 125; 250; 500 mg / kg body weight. Humic acid was administrated by oral gavage for 10 days. After 10 days, spleen organ was taken to preparat by paraffin methode and hematoxilin-eosin staining. Humic acid administration of 125 mg/kg showed a larger diameter than the white pulp isoprinosin control. However, humic acid administration of 500 mg/kg caused the excess stimulation of white pulp of the spleen congestion symptoms characterized by disarrangenment of lymphocytes cluster. There are no significant different of spleen weight between all treatments
AKTIVITAS ANTIFUNGI EKSTRAK METANOL DAUN SALAM (Syzygium polyanthum [Wight] Walp.) TERHADAP PERTUMBUHAN Malassezia sp. (M1) SECARA IN VITRO Sulistrioningsih, Sulistrioningsih; Wardoyo, Elvi Rusmiyanto Pancaning; Kurniatuhadi, Rikhsan
Jurnal Protobiont Vol 9, No 2 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i2.45849

Abstract

Malassezia sp. is a normal flora on the skin of humans and animals. A considerable amount of the fungus may cause tinea versicolor and dandruff. One of the plants that is known to have antifungal compounds is bay leaf (Syzygium polyanthum [Wight] Walp.). This research was done to determine the ability and the Minimum Inhibitory Concentration (MIC) of methanol extract from bay leaves S. polyanthum in inhibiting the growth of Malassezia sp. (M1). This study used a completely randomized design (CRD) with  treatment levels that consisted of concentrations of 80%, 90%, and 100%, negative control (sterile distilled water) and positive control (ketoconazole 2%). The antifungal activity test was carried out using the Kirby-Bauer method. The data showed that each treatment levels had different results. Concentrations of 90% and 100%, and positive control showed inhibition zones of 9.27 mm, 11.57 mm, and 16.67 mm, respectively. Therefore the concentration of 90% was the Minimum Inhibitory Concentration (MIC) of methanol extract from bay leaves S. polyanthum against the growth of Malassezia sp. (M1).
ANGKA PALING MUNGKIN DAN DETEKSI COLIFORM PADA SAMPEL LALAPAN DAUN KEMANGI (Ocimum bacilicum) DI KOTA PONTIANAK Sari, Ita Purnama; Rahmawati, Rahmawati; Kurniatuhadi, Rikhsan
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36822

Abstract

Ocimum basilicum is one type of vegetables that can be consumed without having to be processed. Food consumed must have high nutritional value, clean and free of microbes. Coliform is one of the bacteria that can cause disease in humans. This study aims to determine the value of MPN on samples Ocimum basilicum leaves from warung lamongan in Pontianak and determine the existence of coliform bacteria on Ocimum basilicum leaf samples from warung lamongan in Pontianak. Detection of coliform bacteria using the MPN method. Detection of E. coli was carried out using EMBA media. The results showed that all samples had interval values between 210 MPN/g to > 1100 MPN/g. It showed that all samples excced the threshold determined by BPOM (2016), which was the maximum limit of coliform bacteria contamination in ready-toconsume vegetables was < 3 APM/g. The results of the identification of the bacterial genus coliform grouphad the members of genera Escherichia and Citrobacter
EFEKTIVITAS Pseudomonas sp. BOT4 DALAM MENDEGRADASI MINYAK JELANTAH MENGGUNAKAN SUMBER NITROGEN NATRIUM NITRAT DAN YEAST EXTRACT Harfan, Donatus Tia; Rousdy, Diah Wulandari; Kurniatuhadi, Rikhsan
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36872

Abstract

Jelantah is the residual waste of cooking oil. The disposal of untreated waste directly into the environment has the potential to promote damage such as water channels clogging and water body pollution. Form of waste treatment such as biodegradation can be done using potential bacteria such as Pseudomonas spp. which has been known for being effectively in decomposing organic waste. This study aimed to observe the ability of Pseudomonas sp. BOT4 in degrading jelantah with different nitrogen sources i.e. NaNO3 and yeast extract. This study was carried out from August to October 2018. The used cooking oil samples were homemade with deep frying method and the isolate samples were collected from used cooking oil-contaminated sewer water. Split plot design was used with time of incubation as main plots and nitrogen sources as subplots. The parameters observed were cell density and degraded oil weight. The results obtained stated that nitrogen sources of NaNO3 and yeast extract given optimum effect on cell density of Pseudomonas sp. BOT4 on day three each with OD600 value of 1,361 and 2,300. Nevertheless both nitrogen sources did not really give real effect on final weight of degraded oil, each with weight of 1,28 g dan 1,09 g.
Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro Rikhsan Kurniatuhadi, Ines S Simatupang Elvi Rusmiyanto PW,
Jurnal Protobiont Vol 8, No 2 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i2.34056

Abstract

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%
Hubungan Keberadaan Koloni Bakteri Staphylococcus dan Faktor Fisikawi dalam Ruangan Terhadap Kejadian Sick Building Syndrome (SBS) pada Petugas Perpustakaan Universitas Tanjungpura Rikhsan Kurniatuhadi, Nuriani, Rahmawati,
Jurnal Protobiont Vol 6, No 3 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i3.22486

Abstract

Sick Building Syndrome (SBS) is a group of symptoms experienced by building occupants associated with time spent in buildings that have poor air quality. This research aims to determine the correlation between the factor of the presence of bacteria members of the genus Staphylococcus and indoor physical factors including air temperature, air humidity and light intensity and the occurrences of SBS among library staff of Tanjungpura University. This study used explanatory research method with cross sectional approach conducted from February to July 2017. SBS respondents data were collected using a questionnatres and in depth interviews. In total, 19 respondents were included in this study. The result of analysis of analysis show that there was no correlation between presence of colony of Staphylococcus and most aspects of physical work environment except air humidity p value = 0.003(p value ≤ 0.05) and accurences of SBS at the Library of Tanjungpura University.
Aktivitas Biologis Asap Cair Batang Manggis (Garcinia mangostana L.) Terhadap Viabilitas Streptococcussp. (L.10.3) Rikhsan Kurniatuhadi, Lilis Susanti Elvi Rusmiyanto
Jurnal Protobiont Vol 7, No 3 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i3.29062

Abstract

The wood vinegar of the stem of mangosteen (G. mangostana L.) is the result of pyrolysis of the stem which is condensed into steam. Wood vinegar contains acid and phenol compounds that have antibacterial activities. This research aims to determine the biological activities of the wood vinegar of the stem of mangosteen (G. mangostana L.) on the viability of Streptococcus sp. (L.10.3) and determine the concentration of wood vinegar of the stem of mangosteen (G. mangostana L.) which is effective in inhibiting the growth of Streptococcus sp. (L.10.3). This research used a completely randomized design (CRD) with 6 treatments consisting of wood vinegar with a concentration of 0.1 (Tl); 0.5 (T2); 1 (T3) and 1.5% (T4), negative controls namely sterile distilled water (T5), and positive control namely chlorhexidine of 0.2% (T6). The viability test used the dilution method and calculation of the number of colonies using the total plate count (TPC) method, each treatment was repeated four times. The results showed the increasing concentration of wood vinegar of the stem of mangosteen (G. mangostana L.) and the decreasing amount of Streptococcus sp. (L.IO.3). Wood vinegar at concentration 1.5% showed bacterial growth of 6.9 x 105 CFU / ml which was very different from sterile distilled water by 2.5 x 107 CFU / ml with inhibition up to 96.9% and not significantly different from chlorhexidine of 0.2%.
DETEKSI BAKTERI Salmonella DAN Shigella PADA MAKANAN BURGER DI SUNGAI RAYA DALAM PONTIANAK Apriani, Lisa; Rahmawati, Rahmawati; Kurniatuhadi, Rikhsan
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36836

Abstract

Burger is one of the food in Indonesia that consists of bread, meat, vegetables, sauce, and mayonnaise. This study aims to detect the bacterial presence from genus Salmonella and Shigella in burger food sold in Sungai Raya Dalam, Pontianak. Samples were collected from the location consist of cooked whole burger, precooked bread, postcooked bread, precooked burger meat, postcooked burger meat, lettuce, tomatoes, cucumbers, sauce, and mayonnaise ware bought from six traders. Based on the research, sixty samples tested in Salmonella Shigella Agar (SSA) media showed that 28,33% samples was contaminated with by Salmonella and 3,33% samples was contaminated by Shigella, this showed that burger samples in Sungai Raya Dalam Pontianak could be contaminated by bacteria members of the genus Salmonella and Shigella and exceed the threshold that has been determined by SNI is negative / 25g
Deteksi Bakteri Selulolitik pada Kotoran Luwak (Paradoxurus hermaphroditus) dari Kebun Binatang Bandung Rikhsan Kurniatuhadi, Shinta Rahayu Rahmawati,
Jurnal Protobiont Vol 7, No 2 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i2.25294

Abstract

Cellulolytic bacteria were a group of bacteria involved in coffee fermentation in the digestive tract of civet (Paradoxurus hermaphroditus). The aim of this research was to detect the presence of cellulolytic bacteria isolated from civet feces. This research was conducted from December 2015 to February 2016. Samples of civet feces were collected from Bandung Zoo. Bacteria were isolated from civet feces and screened for cellulolytic activity in Carboxy Methyl Cellulose (CMC) media. Positive result for cellulose producing activity was indicated by the formation of clear zone around the colony. Characterization of the isolated bacteria was done based on macroscopic and microscopic observations followed by biochemical test. A total of 9 genera of cellulolytic bacteria were identified from the civet feces samples, such as Xylophilus (BSKL 1), Caryophanon (BSKL 2), Aeromicrobium (BSKL 3), Exiguobacterium (BSKL 4, BSKL 11), Brochotrix (BSKL 5), Alcaligenes (BSKL 6), Alteromonas (BSKL 7), Halomonas (BSKL 8), Chromobacterium (BSKL 9), Corynebacterium (BSKL 10, BSKL 13), Cellulomonas (BSKL 14) and BSKL isolates 12.
Co-Authors Abdullah Abdullah Anzani, Yunita Magrima Apriani, Lisa Ari Hepi Yanti Ari Hepi Yanti Asrin, Fauzan Diah Wulandari Rousdy Dina Komara Edi Kurniadi Elvi Rusmiyanto, Elvi Fahruna, Yulyanti Firman Saputra Hadary, Ferry Harfan, Donatus Tia Hendri Hendri Holyness Nurdin Singadimedja Indah Permatasari Irwan Lovadi Ita Purnama Sari, Ita Purnama Junardi Junardi Junardi Kustiati Kustiati Larasaty, Suprih Lastyanti Mulyani Masnur Turnip Mukarlina, Mukarlina Muthahhari, Morteza Rafdinal, Rafdinal Rahmawati Rahmawati Rahmawati, Rahmawati Riyandi Riyandi Riza Linda, Riza selno, Silvester Siti Ifadatin Sulistrioningsih, Sulistrioningsih Tri Rima Setyawati Turnip, Masnur Wardoyo, Elvi Rusmiyanto Pancaning Zulfa Zakiah