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All Journal Indonesian Journal of Pharmaceutical Science and Technology Jurnal Kimia Riset Kartika Jurnal Ilmiah Farmasi Jurnal Agrotek UMMat Journal of Pharmacopolium Jurnal Kartika Kimia JURNAL FARMASI GALENIKA Jurnal Farmagazine Jurnal Sains dan Teknologi Farmasi Indonesia Journal of Public Health Concerns
Budiana, Wempi
Sekolah Tinggi Farmasi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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The Effect of Extraction Condition on The Polyphenol Content and Antioxidant Activity of Curcuma zedoaria (Christm.) Roscoe Rhizome Marliani, Lia; Budiana, Wempi; Anandari, Yonara
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 2 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.314 KB) | DOI: 10.15416/ijpst.v4i2.12770

Abstract

White turmeric (Curcuma zedoaria (Christm.) Roscoe) is one of Indonesian herbal medicine. The extraction process to get active compound was influenced by some factor such as solvent, temperature, time, and method of extraction. The objective of this study was to determine the significant factor of extraction  that is solvent, temperature and time of extraction on the polyphenol content and antioxidant activity of white turmeric (Curcuma zedoaria (Christm.) Roscoe) rhizome. Extraction was done by dynamic maceration method with variations (23 factor variable design) of solvent (ethanol 95% and water), temperature (25°C and 70°C), time (6 and 24 hours). The method of analysis of polyphenol content using Folin Ciocalteu reagent, and the antioxidant activity using DPPH free radical reduction method. The experiment design and data analysis using software Design Expert ver.10. The result showed that extraction using ethanol 95% at 70°C for 24 hours was gave high polyphenol content and antioxidant activity. Data analysis was showed that polyphenol content and antioxidant activity was influenced only by solvent of extraction. This study indicated that solvent is significant extraction factor for polyphenol content and antioxidant activity of white turmeric (Curcuma zedoaria (Christm.) Roscoe) rhizome. Keywords: Antioxidant, Curcuma zedoaria (Christm.) Roscoe, extraction, polyphenol
Aktivitas antioksidan ekstrak daun tiga genus Artemisia sp dengan metode DPPH serta penetapan kadar total flavonoid, fenol dan karotenoid Budiana, Wempi; Suhardiman, Aris; Roni, Asep; Sumarah, Isnugraheni; Nara, Theodorik Erik
Kartika : Jurnal Ilmiah Farmasi Vol 5, No 2 (2017)
Publisher : Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.786 KB) | DOI: 10.26874/kjif.v5i2.106

Abstract

Abstrak Tumbuhan adalah salah satu penghasil bahan berkhasiat obat salah satunya sebagai antioksidan. Senyawa pada tumbuhan yang bisa berfungsi sebagai antioksidan adalah flavonoid, fenol, dan karotenoid. Penelitian ini bertujuan untuk menetapkan aktivitas antioksidan dan  kadar flavonoid, fenol dan karotenoid total pada ekstrak daun  Artemisia vulgaris L., Artemisia annua  L. dan  Artemisia dracunculus L. Ekstraksi terhadap masing-masing bahan dilakukan secara bertingkat menggunakan alat refluks, pemantauan kandungan senyawa dengan Kromatografi Lapis Tipis (KLT), pengujian aktivitas antioksidan menggunakan DPPH, penetapan kadar flavonoid total menggunakan metode Ordon, penetapan kadar fenol total menggunakan reagen Folin-Ciocalteu dan penetapan kadar karotenoid total menggunakan pelarut n-heksana. Hasil menunjukkan ekstrak etanol daun Artemsia darcunculus L. paling tinggi dalam menghambat radikal bebas dengan nilai IC50 terkecil (32,10±0,10µg/mL), Ekstrak etanol daun Artemisia vulgaris L. (77,19±0,13µg/mL) dan Ekstrak etanol daun Artemisia annua L. (99,46±0,16 µg/mL). Kadar total flavonoid, fenolat dan karotenoid tertinggi pada ekstrak etil asetat daun Artemisia vulgaris L. berturut-turut 12,83 mg QE/100 mg ekstrak, 68,72 mg GAE/100 mg ekstrak dan 10,01 mg BE/100 mg ekstrak. Dapat disimpulkan bahwa ekstrak etanol daun Artemisia darcunculus L. memiliki aktivitas antioksidan paling kuat dan ekstrak etanol daun Artemisia vulgaris L. memiliki kadar flavonoid total, fenolat total dan karotenoid total tertinggi. Kata Kunci: Artemisia sp, antioksidan, flavonoid, fenol, karotenoid. Antioxidant Activities of Leaves Extract Three Genus Artemisia sp Using DPPH Method and Total Content of Flavonoid, Fenol And Carotenoid Abstract Plant is the natural product medicine such as antioxidant. Natural compounds as antioxidants are flavonoids, phenols, and carotenoids. This research was conducted to determinethe antioxidant activities and the levels of total flavonoids, phenols and carotenoids content in Artemisia vulgaris L., Artemisia annua L. and Artemisia dracunculus L. extract. Each sample was extracted gradual reflux method, monitoring of extracts compounds with Thin Layer Chromatography (TLC), antioxidant activities were test using DPPH, determination of total flavonoids content with Ordon method, total phenol content with Folin-Ciocalteu reagent and total carotenoids using n-hexane. Ethanol extract of Leaves Artemisia dracunculus L. was highest in inhibiting free radicals with the smallest IC50 value (32.10±0.10μg / mL), ethanol extract of leaves Artemisia vulgaris L. (77.19 ± 0.13μg / mL) and ethanol extract of leaves Artemisia annua L. (99.46±0.16 μg / mL). The highest total content of flavonoid, phenolate and carotenoid in ethyl acetate extract of leaves Artemisia vulgaris L. were 12.83 mg QE / 100 mg extract, 68.72 mg GAE / 100 mg extract and 10.01 mg BE / 100 mg extract. Ethanol extract of Leaves Artemisia darcunculus L. has the strongest antioxidant activity, ethanolic extract of leaves Artemisia vulgaris L. has highest total flavonoid content, total phenolate and total carotenoid. Keywords:              Artemisia sp, antioxidant, flavonoid, phenolic, carotenoid
AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BUAH KACANG KRATOK (PHASEOLUS LUNATUS) DAN KULIT BUAH KACANG GUDE (CAJANUS CAJAN) DENGAN METODE DPPH SERTA PENETAPAN KADAR TOTAL FLAVONOID DAN FENOL Budiana, Wempi
Journal of Pharmacopolium Vol 1, No 3 (2018)
Publisher : STIKes Bakti Tunas Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.138 KB)

Abstract

Free radicals are molecules or atoms that have one or more unpaired electrons. Free radical compounds are very reactive and always try to find an electron pair so that the condition is stable. The presence of free radicals in the body is the cause of various chronic and degenerative diseases. This study aimed to determine the antioxidant activity and total flavonoid content and total phenolics from plant extracts of rind Phaseolus lunatus and Cajanus cajan. Extraction was carried out by using reflux method using solvents in stages, n-hexane, ethyl acetate, and ethanol. Evaluation of antioxidant activity was carried out by TLC and visible UV-Vis spectrophotometry with DPPH method. Determination of total flavonoid content, total phenolics using UV-Vis spectrophotometry. The results of antioxidant activity testing, the total content of flavonoids and phenols showed that ethanol extract of Cajanus cajan rind was the best with IC50 values of 22.07 µg / mL, 2.39 mg QE / 100 mg, 9.83mg GAE / 100 mg. The conclusion was that the ethanol extract of rind of Cajanus cajan had very strong antioxidant activity. 
PENGARUH PELARUT, WAKTU DAN SUHU EKSTRAKSI TERHADAP KANDUNGAN SENYAWA FLAVONOID DAN KURKUMINOID EKSTRAK RIMPANG TEMU PUTIH (Curcuma zedoaria (Christm.) Roscoe) Marliani, Lia; Anandari, Yonara; Budiana, Wempi
JURNAL FARMASI GALENIKA Vol 4 No Edisi Khus (2017): Jurnal Farmasi Galenika Volume 4 Edisi Khusus SemNas Tanaman Obat Indon
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.212 KB)

Abstract

Temu putih merupakan salah satu anggota family Zingiberaceae yang sering digunakan sebagai obat tradisional di Indonesia. Rimpang temu putih mengandung senyawa aktif seperti kurkuminoid, polifenol, flavonoid, minyak atsiri, terpenoid. Kandungan senyawa dalam ekstrak dipengaruhi beberapa variabel ekstraksi  seperti pelarut, waktu, suhu, maupun metode ekstraksi. Tujuan: Penelitian ini bertujuan untuk menganalisa pengaruh  pelarut, waktu, dan suhu ekstraksi rimpang temu putih (Curcuma zedoaria (Christm.) Roscoe ) terhadap kadar flavonoid dan kadar kurkuminoid ekstrak.  Ekstraksi simplisia dilakukan dengan metode ekstraksi maserasi dinamik dengan menggunakan variasi dengan design faktor variabel 23  meliputi variasi pelarut (etanol 95% dan air), suhu (25oC dan 70oC) dan waktu (6 jam dan 24 jam). Analisa kandungan senyawa menggunakan Spektrofotometri UV-Visible. Design eksperimen dan analisa data menggunakan software Design Expert ver.10.   Ekstraksi menggunakan pelarut etanol 95% , suhu ekstraksi 70oC dan waktu ekstraksi selama 24 jam menghasilkan ekstrak dengan kadar flavonoid dan kurkuminoid yang paling tinggi. Analisis data menunjukkan bahwa kandungan senyawa flavonoid hanya dipengaruhi oleh jenis pelarut. Sedangkan kandungan senyawa kurkuminoid dipengaruhi oleh ketiga faktor yaitu pelarut, suhu dan waktu ekstraksi.
PENETAPAN KADAR FENOLAT TOTAL, FLAVONOID TOTAL, SERTA AKTIVITAS ANTIOKSIDAN DENGAN METODE DPPH DAN CUPRAC PADA EKSTRAK DAUN SENDOK (Plantago major L.) Budiana, Wempi; Burhanudin, Burhanudin; Roni, Asep
JURNAL FARMASI GALENIKA Vol 3 No 2 (2016): Jurnal Farmasi Galenika Volume 3 No. 2, 2016
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.558 KB)

Abstract

Daun sendok (Plantago major L.) merupakan tanaman gulma yang termasuk ke dalam famili plantaginaceae. Hasil penelitian sebelumnya dengan metode maserasi menggunakan pelarut etanol, fraksi air (IC50 = 80,54 μg/mL), fraksi etil asetat (IC50 = 60,37 μg/mL) dan fraksi n-heksana (IC50 = 80,54 μg/mL). Penelitian ini bertujuan untuk menetapkan kadar  total fenol dan flavonoid dan aktivitas antioksidan dari ekstrak daun sendok. Sampel diperoleh dari dua lokasi tumbuh yang berbeda. Ekstraksi simplisia daun sendok dilakukan dengan metode  refluks menggunakan tiga pelarut dengan polaritas yang berbeda. Pengujian antioksidan menggunakan metode DPPH dan CUPRAC, dan penentuan kadar  total fenol dan flavonoid menggunakan spektrofotometri UV-sinar tampak. Ekstrak etil asetat daun sendok Bantul (IC50 16,81± 0,11 μg/mL) paling rendah dalam menghambat radikal DPPH dibandingkan dengan ekstrak etil asetat sendok Garut (IC50 17,79± 0,40 μg/mL) dan ekstrak etanol Bantul (EC50 20,83 ± 0,07 μg/mL) memiliki nilai paling rendah dalam mereduksi CUPRAC dibandingkan dengan ekstrak etanol daun sendok Garut (EC50 25,60 ± 0,11 μg/mL). Ekstrak etil asetat daun sendok memiliki aktivitas antioksidan paling kuat karena memiliki kadar total fenol dan flavonoid paling tinggi. Ekstrak etil asetat dari daun sendok Bantul memiliki kadar fenol total tertinggi (34,803 ± 0,25 mg  GAE/g ekstrak) dan flavonoid (1,440 ± 0,01 mg QE/g ekstrak). Kesimpulan lokasi tumbuh tanaman mempengaruhi kandungan senyawa aktif, dimana tempat tumbuh yang rendah memiliki aktivitas yang lebih kuat.
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK MIKROALGA Porphyridium cruentum MENGGUNAKAN METODE PEREDAM RADIKAL BEBAS DPPH juliana, Vina; Budiana, Wempi; Y, Aghni Khaeratul Zannah
Journal of Pharmacopolium Vol 3, No 3 (2020)
Publisher : P3M STIKes Bakti Tunas Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36465/jop.v3i3.656

Abstract

The Effect of Extraction Condition on The Polyphenol Content and Antioxidant Activity of Curcuma zedoaria (Christm.) Roscoe Rhizome Lia Marliani; Wempi Budiana; Yonara Anandari
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 2 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.314 KB) | DOI: 10.15416/ijpst.v4i2.12770

Abstract

White turmeric (Curcuma zedoaria (Christm.) Roscoe) is one of Indonesian herbal medicine. The extraction process to get active compound was influenced by some factor such as solvent, temperature, time, and method of extraction. The objective of this study was to determine the significant factor of extraction  that is solvent, temperature and time of extraction on the polyphenol content and antioxidant activity of white turmeric (Curcuma zedoaria (Christm.) Roscoe) rhizome. Extraction was done by dynamic maceration method with variations (23 factor variable design) of solvent (ethanol 95% and water), temperature (25°C and 70°C), time (6 and 24 hours). The method of analysis of polyphenol content using Folin Ciocalteu reagent, and the antioxidant activity using DPPH free radical reduction method. The experiment design and data analysis using software Design Expert ver.10. The result showed that extraction using ethanol 95% at 70°C for 24 hours was gave high polyphenol content and antioxidant activity. Data analysis was showed that polyphenol content and antioxidant activity was influenced only by solvent of extraction. This study indicated that solvent is significant extraction factor for polyphenol content and antioxidant activity of white turmeric (Curcuma zedoaria (Christm.) Roscoe) rhizome. Keywords: Antioxidant, Curcuma zedoaria (Christm.) Roscoe, extraction, polyphenol
UJI AKTIVITAS ANTIBAKTERI TUMBUHAN GANDARIA (Bouea macrophylla Griff) TERHADAP Staphylococcus aureus DAN Escherichia coli Asep Roni; Zahra Sayyidatunnisa; Wempi Budiana
Jurnal Farmagazine Vol 6, No 1 (2019): Jurnal Farmagazine
Publisher : STF Muhammadiyah Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47653/farm.v6i1.126

Abstract

Meski pemanfaatan tanaman sebagai bahan obat telah dilakukan sejak dulu, namun hingga sekarang belum semua tumbuhan telah diketahui potensinya sebagai tanaman obat.Penelitian ini bertujuan untuk mengetahui aktifitas dan senyawa antibakteri dari daun dan batang tanaman gandaria (Bouea macrophylla Griff)., metodelogi yang di unakan meliputi Ekstraksi dilakukan secara bertingkat dengan metode refluks dengan Uji aktivitas antibakteri dilakukan dengan metode mikrodilusi dan bioautografi terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil pengujian menunjukkan nilai KHM ekstrak n-heksana ektrak etil asetat, dan ektrak methanol daun gandaria terhadap Staphylococcus aureus 128 µg/mL, dan nilai KHM pada ekstrak n-heksana batang gandaria yaitu 64 µg/mL, hasil pengujian terhadap bakteri Escherichia coli menunjukan nilai KHM dari ekstrak n-heksana, ekstrak etil asetat dan ekstrak methanol daun gandaria secara berturut-turut yaitu 128 µg/mL, 16 µg/mL dan 16 µg/mL. Hasil tersebut menunjukan ekstrak yang paling aktif yaitu ekstrak etil dan ekstrak methanol batang gandaria. Hasil pengujian bioautografi terhadap ekstrak batang dan daun gandaria menunjukan senyawa fenolat yang diduga sebagai senyawa aktif antibakteri.
Antibacterial Activity of Marine Microalgae Navicula salinicola Extract Against Propionibacterium acnes and Staphylococcus epidermidis Dewi Kurnia; Fitri Bella Mustika Sari; Wempi Budiana
Jurnal Kartika Kimia Vol 3 No 2 (2020): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v3i2.65

Abstract

Navicula salinicola merupakan salah satu jenis mikroalga laut yang termasuk dalam kelas Bacillariophyceae (diatom). Mikroalga ini bersifat bentik dan memiliki warna agak kecoklatan. Pada penelitian ini dilakukan uji aktivitas antibakteri dari mikroalga Naviculla salinicolla yang diekstraksi menggunakan beberapa pelarut organik. Adanya aktivitas antibakteri dilihat melalui nilai KHM dan KBM dari masing-masing ekstrak serta mengidentifikasi golongan senyawa aktif yang terkandung dalam ekstrak tersebut. Ekstraksi dilakukan dengan metode maserasi bertingkat menggunakan pelarut n-heksana, kloroform, dan etanol. Uji aktivitas antibakteri dilakukan terhadap bakteri Propionibacterium acnes dan Staphylococcus epidermidis menggunakan metode mikrodilusi dengan klindamisin sebagai standar pembanding. Hasil penelitian menunjukkan ekstrak kloroform memiliki aktivitas antibakteri dengan nilai KHM 1.024 µg/mL dan nilai KBM >16.384 µg/ml. Pemantauan ekstrak kloroform menunjukkan adanya senyawa alkaloid, fenol, flavonoid, steroid dan terpenoid. Hasil pengujian bioautografi terhadap ekstrak kloroform Navicula salinicola menunjukan bahwa golongan senyawa fenol dan flavonoid memberikan aktivitas antibakteri. Kata kunci: aktivitas antibakteri, Naviculla salinicola, Propionibacterium acnes, Staphylococcus epidermidis
MOLECULAR DOCKING COMPOUNDS IN METHANOL EXTRACT OF MANGO LEAVES (Mangifera indica L.) AS ANTI-INFLAMMATORY AGENT Vina Juliana Anggraeni; Purwaniati Purwaniati; Wempi Budiana; Thoriq Nurdin
Jurnal Kimia Riset Vol. 7 No. 1 (2022): June
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v7i1.35950

Abstract

Previous studies have shown that mango has anti-inflammatory properties. Anti-inflammatory drugs are compounds or medicines used to treat diseases caused by inflammation. The most commonly used anti-inflammatory drug is a non-steroidal anti-inflammatory drug (NSAID). The drug works by inhibiting the enzyme cyclooxygenase (COX). The purpose of this study was to determine the profile metabolites present in the methanol extract of mango leaves and their interaction with the COX enzyme. This study includes an analysis of the compounds contained in the methanol extract of mango leaves using LCMS / MS and molecular docking studies of these compounds. Compounds detected by MS include C26H24O14, C45H84O14, Khellol-β-D-glucoside, Mangiferin, and Nevadensin-5-β-D-glucoside. Analysis of docking result was based on ΔG and Ki and the binding interactions that occur. Compounds that are compatible with COX1 and COX2 are Khellol βD glucoside with G and Ki values of 7.49 kcal/mol and 3.23 µM and 8.32 kcal/mol and 0.7919 µM, respectively. Through the molecular docking process, it was confirmed that khellol β-D-glucoside may be activated as an anti-inflammatory agent.  
Co-Authors Anandari, Yonara Aris Suhardiman Aris Suhardiman Aris Suhardiman Burhanudin Burhanudin Dewi Kurnia Diana Ulfah Ela Fitri Nuryana Fitri Bella Mustika Sari Herlina Husein Hikmiah Hikmiah Inggrid Dirgahayu juliana, Vina Lia Marliani Maria Ulfah Jamil Marliani, Lia Nara, Theodorik Erik Purwaniati Purwaniati Rahayu, Sri Mulyati Roni, Asep Suhardiman, Aris Sumarah, Isnugraheni Thoriq Nurdin Vina Juliana Anggraeni Y, Aghni Khaeratul Zannah Yonara Anandari Zahra Sayyidatunnisa