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Journal : Buletin Peternakan

SRY Gene Marker Differences in Native and Crossbreed Cattle Tety Hartatik; Dwi Ahmad Priyadi; Ali Agus; Sigit Bintara; I Gede Suparta Budisatria; Panjono Panjono; Budi Prasetyo Widyobroto; Yudi Adinata
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.29940

Abstract

This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.
The Effect of Young Coconut Husk on the Quality of Goat Manure-Chicken Excreta Bioculture Dwi Ahmad Priyadi; Dyah Triasih; Sefri Ton; Ari Istanti
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.51434

Abstract

The negative impact of chemical fertilizers use is soil fertility declining. The situation occurs because of biological, physical, and chemical properties of the soil is decreased. Agricultural waste is a large commodity which utilization can still be optimized, for example by using as raw material for organic liquid fertilizer. Agricultural wastes that have good quality as fertilizer include goat manure, chicken excreta, and coconut husk. The utilization of agricultural waste as organic fertilizer is one way of creating sustainable agriculture. This study aims to test the quality of liquid fertilizer (bioculture) of goat manure and chicken excreta by adding various levels of coconut husk. Bioculture is made by anaerobic fermentation for 21 days. The parameters observed included levels of C-organic, N, P, and K bioculture, as well as germination tests to determine the presence of phytotoxins. The data were analyzed using one way ANOVA. The treatment of P5 (5% coconut husk) showed the highest levels of N and P, while the K content was not significantly different from the treatment of P4 (2.5% coconut husk). The germination test showed no phytotoxin activity in bioculture.
Utilization of Different Concentration Sugarcane Molasses to the Quality of Goat Feces-Chicken Excreta-Coconut Husk Organic Liquid Fertilizer Dwi Ahmad Priyadi; Sefri Toni; Ari Istanti
Buletin Peternakan Vol 47, No 1 (2023): BULETIN PETERNAKAN VOL. 47 (1) FEBRUARY 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i1.80378

Abstract

Indonesia is an agricultural country that certainly has the potential for agricultural waste and by-products. Some of those in Indonesia are livestock manure, coconut husk, and sugarcane molasses. Utilization of this waste is limited, which is causing various environmental problems such as environmental pollution. The study is aimed to determine the quality of organic liquid fertilizer made by raw material from goat feces-chicken excreta-coconut husk with various sugarcane molasses concentrations (P0= 0%; P1= 5%; P3= 15%; P4= 20%; P5= 25%). The fermentation was carried out anaerobically for 21 days. The parameters of temperature, pH, organoleptic (color and odor), levels of C-organic, nitrogen (N) total, phosphorus (P) total, potassium (K) total, and biological tests were observed. Biological tests were conducted in the growth of mung bean (Vigna radiata) and spinach (Amaranthus tricolor). Data were analyzed using one-way ANOVA. Variations in the addition of molasses sugarcane did not affect the liquid fertilizer's temperature and pH during fermentation. However, the treatments had positive effect on organoleptic test, C-organic, N total, P total, K total, and biological tests. The addition of 15% sugarcane molasses (P3) showed a dominating performance toward most of the parameters tested. The P3 treatment produced liquid fertilizer with a pleasant smell, and the contents of C-organic, total N, total P, and total K were 2.12%, 0.25%, 0.13%, and 0.13%,  respectively. and produce spinach as a biological test with plant height, number of leaves, leaf width, stem diameter, and root length were 21.82 cm, 6.66 sheets, 3.59 cm, 4.09 mm, and 14.67 cm, respectively.