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Isolation, Characterization and Activity Test of Soil Origin Bacteria Amilage Monica Kharisma Swandi
Biosfer: Jurnal Tadris Biologi Vol 11, No 2 (2020): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

This study aimed to obtain amylase-producing potent bacteria from soil and test the amylase activity produced. Soil samples were taken from the Biological Education and Research Forest, Andalas University. The isolation was done by using the stratified dilution technique on agar media. The screening of amylase activity employed the qualitative and quantitative tests on agar starch. From 8 isolated amylolytic bacteria, there were three isolates with amylolytic potential. The results of characterization and identification based on Bergey's Manual of Determinative Bacteriology show that isolates A4, A1, and A6 belonged to the genus Bacillus, Corynebacterium, and Klebsiella. The bacteria obtained can then be produced and optimized for the needs of industrial enzymes.

Diversity of Medicinal Plant and Potential as Antibacterial in Peret Hill Forest, Tiang Tarah Village, District of Bakam, West Bangka Monica Kharisma Swandi; Neli Sulastri; Fitri Ulandari; Mega Febriyanti
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111785-0-00

Traditional medicine that has been done by the Tiang Tarah Village shaman, Bakam District, Bangka Regency uses plants as medicine to cure various diseases. However, so far some plants that are used as medicine can not be proven definitively effectiveness against a disease. Therefore, this research was conducted with the aim of knowing which plants are used as traditional medicine in Tiang Tarah Village, Bakam District, Bangka Regency, exploring the diversity of medicinal plants in the Bukit Peret Forest, and testing the antibacterial activity of medicinal plants against Escherichia coli and Staphylococcus aureus bacteria. The research was conducted with several stages, namely: (i) Interviewing informants who are experienced in the field of traditional medicine (shaman) using snowball sampling techniques, (ii) Exploration in the Peret Hill Forest for sampling using roaming method, (iii) Antibacterial activity test medicinal plants against E. coli and S. aureus by using agar diffusion method. The results of the study obtained 22 types of medicinal plants interviewed by 3 shamans Tiang Tarah Village. Of the 22 species, only 14 were found during exploration in the Peret Hill Forest. 10 of them have antibacterial activity against E. coli and 11 types against S.aureus.

Isolasi Bakteri Pendegradasi Limbah Cair Industri Minyak Sawit Monica Kharisma Swandi; Periadnadi Periadnadi; Nurmiati Nurmiati
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

An isolation of degrading bacteria of Palm Oil Mill Effluent (POME) was conducted from May to October 2014 at the Laboratory of Microbiology, Department of Biology, Faculty of Natural Sciences, Andalas University. This study aimed to describe the characteristic of the bacteria which can degradate the POME. The result obtained five isolates ie BLS1, BLS2, BLS3, BLS4 and BLS5 and their characters were rod-shaped (basil), gram-positive, motile, positive catalase and forming endospores. Keywords: isolation, characterization, degrading bacteria, POME

Uji Organoleptik dan Umur Simpan Soyghurt dengan Berbagai Konsentrasi Gula dan Waktu Inkubasi Firsty Vanezza Gabriela; Frista Chairunnisa; Nadhifah Raniah; Rama Pratama; Monica Kharisma Swandi; Nur Azizah
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 6 No 2 (2021): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

One of fermented soybean (Glycine max L. Merr) products is soy milk, called as Thew Fu Sui by the community of Bangka Belitung. Soy milk product has unpleasant aroma or “langu” and need to be processed into alternative product such as yogurt. Soy milk yogurt (soyghurt) was made by utilizing the same bacteria in yogurt-making from animal milk including Streptococcus thermophillus and Lactobacillus bulgaricus. Sugar concentration and incubation time were the factors that influenced the fermentation process and product’s taste. This research was conducted to determine the level of people's preference for soyghurt by organoleptic assessment of color, taste, aroma and texture based on variation of sugar concentration and incubation time of soyghurt. Our study found that various additional sugar concentration and incubation time gave difference result in organoleptic test. Soyghurt with 10%, 12.5%, and 15% of sugar addition became more favorable among the participant. The incubation time of 8 hours, 12 hours, and 16 hours equally produced good fermentation results. Storage in refrigerator for 1 days could maintain its flavor, texture, colour and aroma, but changed after 2 days stored.

Training on Making Fresh and Preserve Slide-Culture Preparation and Identification of Microscopic Fungi for Senior High School Biology Teachers Monica Kharisma Swandi; Salmi Salmi
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 6, No 2 (2022): Vol 6, No 2 (2022)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.021 KB)

Fresh and preserve slide-culture preparation and identification of microscopic fungi workshop for biology teachers in senior high school was conducted as the community service activity. The aim of this workshop was to improve teachers’s competency by providing a short lecture and training on fresh and preserve slide-culture preparation and identification of microscopic fungi. This workshop was held online using zoom meeting application and divided into four session; short lecture, group activity for identification of fungi, discussion and evaluation. The participant’s knowledge about the topic evaluated two times, before the short lecture and after the discussion session using Google-form. Statistical analysis used paired sample t-test shown that the teachers’s knowledge and skill in identification of microscopic fungi significantly improved after the workshop (p0.05). As the workshop ended, the participant also got a tutorial video, pocketbook and preserved microscopic fungi slide-cluture. We hope the biology teachers can implement all they got during the workshop in learning process at school, so the students can understand the fungi topic better.

Pelatihan Pembuatan Preparat Segar dan Awetan Jamur Serta Cara Mengidentifikasi Jamur Mikroskopik Bagi Guru Biologi Sekolah Menengah Atas Monica Kharisma Swandi; Salmi Salmi
Jompa Abdi: Jurnal Pengabdian Masyarakat Vol. 1 No. 2 (2022): Jompa Abdi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Jompa Research and Development

Kegiatan pelatihan pembuatan preparat segar dan awetan jamur serta cara mengidentifikasi jamur mikroskopik bagi Guru Biologi Sekolah Menengah Atas dilaksanakan dalam rangka pengabdian kepada masyarakat. Kegiatan ini bertujuan untuk meningkatkan kompetensi guru biologi melalui kuliah singkat, pelatihan persiapan preparat segar dan awetan serta identifikasi jamur mikroskopik. Pelatihan dilakukan secara daring menggunakan aplikasi zoom meeting dan dilaksanakan dalam 4 sesi : kuliah singkat, kerja kelompok untuk identifikasi jamur, diskusi, dan evaluasi. Tingkat pengetahuan peserta dievaluasi dua kali, sebelum kuliah singkat dan setelah sesi diskusi dengan menggunakan google-form. Analisis secara statistik menggunakan paired sample t-test menunjukkan bahwa adanya peningkatan pengetahuan dan kemahiran peserta dalam mengidentifikasi jamur mikrokopik secara signifikan (p<0.05). Pada akhir pelatihan, peserta juga mendapatkan video pembelajaran, buku saku dan preparat jamur awetan. Kami berharap apa yang diperoleh guru biologi selama mengikuti workshop dapat diaplikasikan pada proses pembelajaran di sekolah masing-masing, sehingga siswa dapat memahami materi jamur dengan lebih mudah.

Uji Aktivitas Antibakteri Ekstrak Metanol Daun Paku Resam (Glechenia liearis Burm.) pada Tiga Bakteri Penyebab Akne Vulgaris Salmi Salmi; Monica Kharisma Swandi
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 4, No 2 (2022)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v4i2.30474

Abstract: Acne vulgaris or acne is a skin disease caused by a bacterial infection. This study aimed to evaluate the antibacterial activity of methanol extract of paku resam leaves (Gleichenia linearis Burm.) on three different acne-causing bacteria Propionibacterium acnes ATCC 6919, Staphylococcus epidermidis ATCC 12228, and Staphylococcus aureus ATCC 25923. The research consisted of an antibacterial activity test using the disc diffusion method, a minimum inhibitory concentration (MIC) test, a minimum bactericidal concentration (MBC) test, and an equivalence test of antibacterial activity with clindamycin as a positive control. This study found that the methanol extract of paku resam leaves had a significant effect (p<0.05) on inhibiting the growth of the three bacteria at all concentrations tested, namely 2.5; 5; 10; 15; 20; 30%. The MIC value of the extract was lower for P. acnes ATCC 6919 and S. epidermidis ATCC 12228 by 1.63%, compared to S. aureus ATCC 25923 by 3.25%. The KBM value of methanol extract of paku resam leaves was lower for P. acnes ATCC 6919 and S. epidermidis ATCC 12228 (3.25%) compared to S. aureus ATCC 25923 (6.5%). The equivalence test showed that the antibacterial activity of the methanol extract of paku resam did not as strong as clindamycin inhibitory activity to the acne-causing bacteria. In conclusion, the methanolic extract of paku resam leaves has antibacterial activity toward three acne-causing bacteria based on the antibacterial test, MIC, and KBM tests, but its activity was still lessened than clindamycin. Abstrak: Akne vulgaris atau jerawat merupakan salah satu penyakit kulit yang dapat disebabkan oleh infeksi bakteri. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak metanol daun paku resam (Glechenia linearis Burm.) pada Propionibacterium acnes ATCC 6919, Staphylococcus epidermidis ATCC 12228 dan Staphylococcus aureus ATCC 25923 bakteri penyebab jerawat. Pengujian terdiri atas uji aktivitas antibakteri dilakukan dengan metode difusi cakram, uji konsentrasi hambat minimum (KHM), uji konsentrasi bunuh minimum (KBM) dan uji kesetaraan aktivitas antibakteri dengan antibiotik klindamisin sebagai kontrol positif. Hasil penelitian menunjukkan bahwa ekstrak metanol daun paku resam berpengaruh nyata (p<0.05) menekan pertumbuhan ketiga bakteri pada semua konsentrasi yang diujikan yaitu 2.5; 5; 10; 15; 20; 30 %. Nilai KHM ekstrak lebih rendah terhadap P. acnes ATCC 6919 dan S. epidermidis ATCC 12228 sebesar 1.63%, dibandingkan pada S. aureus ATCC 25923 sebesar 3.25%. nilai KBM ekstrak metanol daun paku resam lebih rendah terhadap P. acnes ATCC 6919 dan S. epidermidis ATCC 12228 (3.25%) dibandingkan pada S. aureus ATCC 25923 (6.5%). Hasil uji kesetaraan menunjukkan bahwa aktivitas antibakteri ekstrak metanol paku resam masih lebih rendah dibandingkan klindamisin. Sebagai kesimpulan, ekstrak metanol daun paku resam mampu menghambat pertumbuhan 3 bakteri penyebab jerawat berdasarkan uji antibakteri, KHM dan KBM, namun aktivitasnya masih lebih rendah dibandingkan klindamisin.

Karakteristik Berbagai Formulasi Mikroorganisme Lokal (MOL) dan Pengaruhnya terhadap Pertumbuhan Tanaman Kangkung Darat (Ipomoea reptans (L.) Poir) Monica Kharisma Swandi; Jely Jeniver; Siti Ade Nur Milah; Mutiara Safitri; Inayah Asyyifa; Irawati Irawati; Putri Aliya; Khusnul Khotimah; Astri Dian Sari; Julian Eka Putri; Nadila Puspita Sari; Firzan Fatansyah; Efita Karunia Harita; Lesta Ayu Wiriyanti; Putri Ayu Indah Suryani
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4161

Mikroorganisme lokal (MOL) berisikan larutan hasil fermentasi berbahan dasar dari berbagai sumber daya lokal. Larutan MOL berpotensi dijadikan pupuk organik cair karena mengandung mikroorganisme yang dapat meningkatkan pertumbuhan tanaman. Penelitian ini bertujuan mengetahui karakteristik MOL berdasarkan sumber mikroorganisme yang berbeda yang didapat dari bahan pangan khas Bangka Belitung, serta mengetahui efektivitasnya terhadap pertumbuhan tanaman kangkung darat. Penelitian eksperimen ini menggunakan rancangan acak lengkap (RAL) dua faktorial yaitu jenis MOL (tanpa pemberian MOL (M0), MOL berbahan terasi Bangka (M1), MOL berbahan rusip (M2), dan MOL berbahan sampah dapur (M3)) dan dosis MOL (0% (D0), 15% (D1), 25% (D2), dan 50% (D3)). Masing-masing perlakuan dilakukan sebanyak 2 kali ulangan dengan total 32 unit percobaan. Variabel yang diamati yaitu karakteristik MOL setelah 1 minggu inkubasi dan pengaruh pemberian MOL terhadap pertumbuhan tanaman kangkung darat 14 hari setelah tanam (HST). Data dianalisis secara statistik menggunakan software SPSS versi 25 dengan menggunakan metode analysis of varian (ANOVA) dan uji lanjut duncan’s multiple range test (DMRT) dengan taraf kepercayaan 95%. Hasil penelitian didapatkan semua perlakuan MOL behasil terfermentasi dengan karakteristik beraroma asam, tekstur cair, terdapat gelembung udara, pH asam, dan terdapat bakteri dan kapang. Hasil ANOVA menunjukkan faktor pemberian jenis MOL dan faktor interaksi jenis MOL dan dosis MOL berpengaruh nyata terhadap semua parameter pengamatan. Namun pemberian faktor dosis MOL tidak berpengaruh nyata terhadap semua parameter pengamatan. Perlakuan jenis dan dosis MOL M2D1 dapat meningkatkan respon jumlah daun, bobot basah tanaman, bobot kering tanaman, bobot kering tajuk, dan bobot kering akar tanaman kangkung darat. Sedangkan perlakuan M1D3 meningkatkan respon tinggi tanaman kangkung darat.

Uji Aktivitas Antibakteri Ekstrak Metanol Daun Paku Resam (Glechenia liearis Burm.) pada Tiga Bakteri Penyebab Akne Vulgaris Salmi Salmi; Monica Kharisma Swandi
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 4, No 2 (2022)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v4i2.30474

Abstract: Acne vulgaris or acne is a skin disease caused by a bacterial infection. This study aimed to evaluate the antibacterial activity of methanol extract of paku resam leaves (Gleichenia linearis Burm.) on three different acne-causing bacteria Propionibacterium acnes ATCC 6919, Staphylococcus epidermidis ATCC 12228, and Staphylococcus aureus ATCC 25923. The research consisted of an antibacterial activity test using the disc diffusion method, a minimum inhibitory concentration (MIC) test, a minimum bactericidal concentration (MBC) test, and an equivalence test of antibacterial activity with clindamycin as a positive control. This study found that the methanol extract of paku resam leaves had a significant effect (p<0.05) on inhibiting the growth of the three bacteria at all concentrations tested, namely 2.5; 5; 10; 15; 20; 30%. The MIC value of the extract was lower for P. acnes ATCC 6919 and S. epidermidis ATCC 12228 by 1.63%, compared to S. aureus ATCC 25923 by 3.25%. The KBM value of methanol extract of paku resam leaves was lower for P. acnes ATCC 6919 and S. epidermidis ATCC 12228 (3.25%) compared to S. aureus ATCC 25923 (6.5%). The equivalence test showed that the antibacterial activity of the methanol extract of paku resam did not as strong as clindamycin inhibitory activity to the acne-causing bacteria. In conclusion, the methanolic extract of paku resam leaves has antibacterial activity toward three acne-causing bacteria based on the antibacterial test, MIC, and KBM tests, but its activity was still lessened than clindamycin. Abstrak: Akne vulgaris atau jerawat merupakan salah satu penyakit kulit yang dapat disebabkan oleh infeksi bakteri. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak metanol daun paku resam (Glechenia linearis Burm.) pada Propionibacterium acnes ATCC 6919, Staphylococcus epidermidis ATCC 12228 dan Staphylococcus aureus ATCC 25923 bakteri penyebab jerawat. Pengujian terdiri atas uji aktivitas antibakteri dilakukan dengan metode difusi cakram, uji konsentrasi hambat minimum (KHM), uji konsentrasi bunuh minimum (KBM) dan uji kesetaraan aktivitas antibakteri dengan antibiotik klindamisin sebagai kontrol positif. Hasil penelitian menunjukkan bahwa ekstrak metanol daun paku resam berpengaruh nyata (p<0.05) menekan pertumbuhan ketiga bakteri pada semua konsentrasi yang diujikan yaitu 2.5; 5; 10; 15; 20; 30 %. Nilai KHM ekstrak lebih rendah terhadap P. acnes ATCC 6919 dan S. epidermidis ATCC 12228 sebesar 1.63%, dibandingkan pada S. aureus ATCC 25923 sebesar 3.25%. nilai KBM ekstrak metanol daun paku resam lebih rendah terhadap P. acnes ATCC 6919 dan S. epidermidis ATCC 12228 (3.25%) dibandingkan pada S. aureus ATCC 25923 (6.5%). Hasil uji kesetaraan menunjukkan bahwa aktivitas antibakteri ekstrak metanol paku resam masih lebih rendah dibandingkan klindamisin. Sebagai kesimpulan, ekstrak metanol daun paku resam mampu menghambat pertumbuhan 3 bakteri penyebab jerawat berdasarkan uji antibakteri, KHM dan KBM, namun aktivitasnya masih lebih rendah dibandingkan klindamisin.

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