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All Journal Buletin Penelitian Tanaman Rempah dan Obat PROSIDING SEMINAR KIMIA Biopropal Industri Jurnal Fitofarmaka Indonesia Jurnal Ilmiah Manuntung Jurnal Farmamedika (Pharmamedica Journal) Buletin Penelitian Tanaman Rempah dan Obat
Lilik Sulastri
Sekolah Tinggi Teknologi Industri Dan Farmasi Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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AKTIVITAS ANTIOKSIDAN KECIBELING, BAKAU MERAH, DAN KATUK PADA METODE EKSTRAKSI DAN RASIO EKSTRAK YANG BERBEDA Lilik Sulastri; Ika Oktavia; Partomuan Simanjuntak
Buletin Penelitian Tanaman Rempah dan Obat Vol 31, No 1 (2020): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v31n1.2020.1-7

Abstract

Tumbuhan obat Indonesia, seperti kecibeling {Strobilanthes crispa (L.) Blume}, bakau merah (Rhizophora stylosa Griff.) dan katuk {Sauropus androgynus (L.) Merr.} mengandung senyawa aktif yang berperan sebagai antioksidan. Tujuan penelitian adalah untuk mengetahui pengaruh metode ekstraksi (maserasi dan infusa) dan rasio perbandingan ekstrak daun kecibeling dan bakau merah, serta batang katuk, baik secara tunggal maupun kombinasi terhadap aktivitas antioksidan ekstrak.  Serbuk simplisia kering berukuran 40 mesh dari daun kecibeling, daun bakau merah, dan batang katuk diekstraksi menggunakan pelarut etanol 96 % (metode maserasi) dan dengan pelarut air (metode infusa). Ekstrak tunggal atau kombinasi ekstrak tunggal daun kecibeling, daun bakau merah, dan batang katuk (1:1:1; 1:1:2; 1:2:1; dan 2:1:1) diuji aktivitas antioksidannya berdasarkan metode radikal bebas 2,2-difenil-1-pikrilhidrazil (DPPH). Metode maserasi dengan etanol lebih baik dibandingkan dengan metode infusa dengan air. Antioksidan dari ekstrak etanol daun kecibeling menunjukkan aktivitas paling kuat dengan nilai konsentrasi penghambatan (IC50) sebesar 37,65 ppm dibandingkan dengan ekstrak air. Kombinasi ekstrak etanol tunggal dari daun kecibeling, daun bakau merah, dan batang katuk (2:1:1) bersifat sinergis dengan aktivitas antioksidan paling kuat (IC50= 18,78 ppm), tetapi masih di bawah aktivitas antioksidan vitamin C (IC50 = 4,24 ppm). Ekstrak etanol daun kecibeling secara tunggal atau dikombinasikan dengan ekstrak etanol daun bakau merah dan batang katuk berpotensi dikembangkan sebagai antioksidan.
PENAMBATAN MOLEKULER SENYAWA XANTON PADA KULIT BUAH MANGGIS (Garcinia Mangostana L.) DENGAN ENZIM COX-2 Sebagai Kandidat Antikanker Payudara. Herson Cahaya Himawan; Lilik Sulastri; Verari Vernando
Jurnal Farmamedika (Pharmamedika Journal) Vol 3 No 1 (2018): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.939 KB) | DOI: 10.47219/ath.v3i1.28

Abstract

Breast cancer is one of the most common types of cancer suffered by women around the world. Breast cancer is known that has relationship with chronic inflammation that is controlled by cyclooxygenase-2 (COX-2). Pericarp of mangosteen (Garcinia mangostana L.) contains a class compound of xanthone. The compound has potential as an inhibitor of the COX-2enzyme.The purpose of this research is to know the most active compound of xanthone compounds. Potential of xanthone compounds tested the toxicityby using Toxtree, the analysis of toxicity is focused on the carcinogenycity and mutagenicity.The result of toxicity test of ligand compound with Toxtree software found that the compound of 1-Isomangostin hydrate, 3-Isomangostin hydrate, Euxanthon, BR-xanthone A,Garcimangosone B and Garcimangosone D predicted safe for use as medicine. Then the compound itself analyzed based on the criteria of Lipinski's Rule of Five to predict the drug absorption in the human body. The analysis showed,five compounds fulfilled the criteria of Lipinski except Garcimangosone D. Further analysis was molecular docking using Autodock VinaA had a binding energy (ΔG) the best that was -10.1 kcal / mol if it compared to other test compounds, approaching the binding energy (ΔG) comparative compound of celecoxib -12.4 kcal / mol.
PENGARUH FRAKSINASI BUNCIS (PHASEOLUS VULGARIS L.) TERHADAP PENURUNAN KADAR KOLESTEROL TIKUS PUTIH (RATTUS NORVEGICUS L.) YANG HIPERKOLESTEROLEMIA Lilik Sulastri; Putri Syafalia; Achmad Fauzi Isa
Jurnal Farmamedika (Pharmamedika Journal) Vol 5 No 1 (2020): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v5i1.90

Abstract

Hiperlipidemia merupakan suatu keadaan meningkatnya kadar lipid darah yang ditandai dengan meningkatnya kadar kolesterol total, Low Density Lipoprotein (LDL), dan trigliserida dalam darah yang melebihi batas normal. Sampai saat ini telah banyak obat yang digunakan untuk penanganan hiperlipidemia baik obat sintetik dan obat herbal. Salah satu obat herbal yang telah digunakan oleh masyarakat Indonesia adalah buncis (Phaseolus vulgaris L.). Tujuan penelitian ini adalah untuk mengetahui pengaruh beberapa ekstrak (n-heksan, etilasetat dan air) buah buncis terhadap penurunan kadar kolesterol tikus jantan galur Sprague Dawley yang telah diinduksi kuning telur puyuh, PTU 0,02% dan asupan pakan aterogenik selama 14 hari. Pengukuran kadar kolesterol dilakukan secara enzimatis dengan alat kolesterolmeter Easy Touch®. Hasil fraksinasi ekstrak etanol buncis dengan pelarut n-heksan, etil asetat, dan air diujikan selama 7 hari dengan dosis 50 mg/kg BB diperoleh fraksi air sebagai fraksi yang paling aktif menurunkan kadar kolesterol darah. Pada uji lanjutan fraksi air dilakukan terhadap 5 kelompok perlakuan (5x5 ekor), yaitu kelompok 1 (Na CMC 0,5%) sebagai kontrol negatif, kelompok 2 (simvastatin 0,18 mg/200 g BB) sebagai kontrol positif dan variasi dosis fraksi air 50 mg/kg BB, 100 mg/kg BB serta 150 mg/kg BB (kelompok 3, 4, dan 5). Selama 21 hari perlakuan tikus tetap diberikan pakan aterogenik dan pengukuran kadar kolesterol dilakukan pada hari ke 0, 14, 21, 28, dan 35. Hasil uji lanjutan menunjukkan bahwa fraksi air ekstrak buncis pada dosis 50 dan 100 mg/kg BB tidak memiliki perbedaan yang nyata dengan simvastatin, sedangkan pada dosis 150 mg/kg BB menunjukkan perbedaan yang nyata dangan simvastatin dalam menurunkan kadar kolesterol.
AKTIVITAS EKSTRAK ETANOL KULIT BUAH NANGKA (Artocapus heterophyllus) MENGHAMBAT PERTUMBUHAN BAKTERI Escherichia coli dan Staphylococcus aureus Siti Mariam; Lia Rahmania; Lilik Sulastri
Jurnal Farmamedika (Pharmamedika Journal) Vol 5 No 2 (2020): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v5i2.109

Abstract

Senyawa aktif yang terkadung dalam buah nangka (Artocapus heterophyllus Lamk.) diantaranya tannin dan polifenol yang diketahui memiliki efek antibakteri. Perlu dilakukan penelitian untuk mengetahui komposisi senyawa kimia dan aktifitas antibakteri pada bakteri Staphylococcus aureus (gram positif) dan bakteri Escherichia coli (gram negative) dari limbah kulit buah nangka Ekstraksi kulit buah dengan metode maserasi menggunakan pelarut etanol 96%. Metode difusi cakram digunakan untuk menguji aktivitas antibakteri dengan 3 kelompok perlakuan (terdiri dari kelompok konsentrasi 10%, 20%, dan 30%) serta 2 kelompok kontrol (chloramphenicol base sebagai control positif dan DMSO sebagai kontrol negative). Hasil penelitian menunjukkan bahwa 3 kelompok ekstrak kulit buah nangka mempunyai aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas tertinggi terhadap bakteri Staphylococcus aureus pada konsentrasi 30% menghasilkan nilai diameter zona hambat rata-rata sebesar 10,76 mm termasuk kategori kuat dan pada bakteri Escherichia coli menghasilkan diameter zona hambat rata-rata sebesar 7,13 mm termasuk kategori sedang. Hasil Analisis data menggunakan ANOVA menunjukkan bahwa aktivitas antibakteri ekstrak kulit buah nangka berbeda nyata pada bakteri Staphylococcus aureus dan Escherichia coli. Hasil uji lanjut dengan Uji Duncan menunjukkan ekstrak kulit buah nangka mempunyai aktivitas antibakteri lebih tinggi pada bakteri Staphylococcus aureus dibanding bakteri Escherichia coli.
Aktivitas Fraksi Air Kulit Batang Mahoni (Swietenia macrophylla King.) Dan Studi In Silico Senyawa Kimia Penghambat Enzim α-Glukosidase Arini Khaerunnisa; Ratna Djamil; Lilik Sulastri; Partomuan Simanjuntak
Jurnal Fitofarmaka Indonesia Vol 9, No 1 (2022): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v9i1.807

Abstract

Inhibition of the α-glucosidase can be used as antidiabetic therapy because it can reduce postprandial blood sugar levels. The stem bark of mahogany (Swietenia macrophylla King.) have properties as inhibitors of enzyme α-glucosidase. The purpose of this study was to determine the active compound of mahogany stem bark againstα-glucosidase enzyme in vitro and in silico. 96% ethanol extract of mahogany stem bark was partitioned with n-hexane, ethyl acetate and water. Testing of inhibition of α-glucosidase enzyme work done with substrate p-nitrophenyl-α-D-glucopyranoside. The water fraction was separated on silica gel column chromatography (SiO2 : dichloromethane-methanol = 10 : 1 ~ 1 : 1 and methanol).Molecular docking using AutoDock 4.2.6 was performed to predict the binding modes of α-glucosidase enzyme with chemical compound from water subfraction of stem bark mahogany. The results showed that water fraction of stem bark mahogany have antidiabetic activity with IC50 value of 1.51 µg/mL. Fraction Fr-1 gave an inhibition of 83.61 ± 1.11 %. Fr-1 contains chemical compound catechin, evodionol and swietenitin K. In silico study showed that catechin and evodionol free energy value (ΔG) -6.98 and -6.25 Kcal/mol and inhibition constanta (Ki) 7.61 and 26.18 µM.
AKTIVITAS DAN ISOLASI SENYAWA BIOAKTIF DARI EKSTRAK ETIL ASETAT DAUN TEH (Camellia sinensis (L.) Kuntze) SEBAGAI PENGHAMBATAN ENZIM α-GLUKOSIDASE Nuraini Saadah; Lilik Sulastri; Syamsoedin Abdilah; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Tea (Camellia sinensis (L.) Kuntze) is one of the plants that has compounds that have the potential as inhibitors of the α-glucosidase enzyme. The purpose of this study was to know the activity of chemical compounds in the ethyl acetate extract of tea leaves as an inhibitor of the α-glucosidase enzyme. Extraction tea leaf was carried out by maceration tea leaf powder in ethanol 96% 3 times, then partitioned with ethyl acetate and water. The ethyl acetate extract obtained was determined by the inhibitory activity of the -glucosidase enzyme, then fractionated by column chromatography (SiO2; n-hexane: ethyl acetate 10:1 ~ 1:1) yielding 10 fractions (CSEA-1 ~ CSEA-10). The CSEA-2 fraction was further fractionated by column chromatography (SiO2; n-hexane: ethyl acetate 20:1 ~ 1:1) yielding 9 fractions (CSEA-2.1~ CSEA-2.9). The results showed that the ethyl acetate extract had activity with an IC50 value of 45.04 ppm, the fractionated CSEA-2.1 subfraction had an IC50 value of 41.51 ppm with acarbose as a comparison of 19.24 ppm. The conclusion is that the CSEA-2.1 subfraction has better activity than the extract in inhibiting the α-glucosidase enzyme and can be used as an alternative candidate for diabetes drugs. Keywords: Camellia sinensis (L.) Kuntze, tea, α-glucosidase enzyme inhibitor, Indonesian medicinal plant, IC50
AKTIVITAS DAN ISOLASI SENYAWA AKTIF PENGHAMBAT ENZIM ALFA-GLUKOSIDASE DARI EKSTRAK ETIL ASETAT DAUN STEVIA (Stevia rebaudiana Bertoni) SECARA IN VITRO Laura Stephanie Joner; Lilik Sulastri; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Stevia leaves (Stevia rebaudiana Bertoni) is a plant that commonly used as a natural sweetener to replace sugar. According to research, stevia has many health benefits and one of them is antidiabetic. The aims of this research are to determine the activity of the ethyl acetate extract of stevia leaves (Stevia rebaudiana Bertoni) as an inhibitor of -glucosidase enzyme. The stages on this research include extraction with 96% ethanol solvent, then the ethanolic extract was partitioned with ethyl acetate – water = 1:1. Isolation and purification of the ethyl acetate extract was carried out by column chromatography (SiO2; i). n-hexane – etil acetate = 10 : 1 ~ 1 : 1 ii). n-hexane – etil acetate = 20:1 which was guided by testing the inhibitory activity of α-glucosidase enzyme by in vitro studies. The results showed that the STEA-1.1 fraction had the best activity with IC50 value 39,55 ± 0,51 ppm compared to the partitioned ethyl acetate extract with an IC50 value 43,47 ± 0,05 ppm, while the acarbose had an IC50 value 19,24 ± 0,03 ppm. Keywords: Stevia rebaudiana Bertoni, α-glucosidase, Antidiabetic, Indonesian Medicinal Plants, IC50
AKTIVITAS SENYAWA BIOAKTIF DARI EKSTRAK ETIL ASETAT DAUN YAKON (Smallanthus sonchifolius) SEBAGAI PENGHAMBAT ENZIM α-GLUKOSIDASE Riska Diana; Lilik Sulastri; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Yakon (Smallanthus Sonchifolius) leaves in Indonesia, better known as insulin leaves, scientifically have various properties and one of them is antidiabetic. This study aims to determine the activity of secondary metabolites contained in the ethyl acetate extract of yakon (Smallanthus Sonchifolius) leaf as an inhibitor of the -glucosidase enzyme. Yakon leaf ethyl acetate extract using eluent from TLC results. Until several sub-factions are obtained. These sub-fractions were combined in TLC and tested for activity. The sub-fraction with the best activity in Back column chromatography and TLC and tested until the IC50 value is obtained. The results showed that the ethyl acetate extract of yakon leaves had inhibitory activity of the -glucosidase enzyme with an IC50 of 45,67 ppm. The results of column chromatography separation showed that sub-fraction 6.5 was a sub-fraction with an IC50 value of 42,23 ppm. Conclusion: Yakon leaf ethyl acetate extract had better activity than purified F6.5 and acarbose as a positive control with an IC50 value of 21,36 ppm. Keywords : Smallanthus sonchifolius, α -glucosidase inhibitory enzyme, IC50, Indonesian medicinal plant
AKTIVITAS PENGHAMBATAN ENZIM α-GLUKOSIDASE EKSTRAK DAN FRAKSI ETIL ASETAT DAUN SALAM (Syzygium polyanthum (Wight) Walp.) Aufa Ali Fajar Nanda Nasukha; Lilik Sulastri; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Diabetes Mellitus is a chronic disease caused by the inability of the pancreas to produce insulin which is characterized by hyperglycemia. One of the therapies used to lower blood glucose levels is drugs that have a mechanism to inhibit the α-glucosidase enzyme, so that it can delay the glucose absorption in the digestive tract. One of the plants that has the potential to inhibit the α-glucosidase enzyme is bay leaf. The intention of this study was determine the activity of ethyl acetate extract and chromatographic fraction of bay leaf in inhibiting the α-glucosidase enzyme. A liquid-partition process from 96% ethanol extract was carried out to obtain ethyl acetate extract. The ethyl acetat extract was separated by fractionation using chromatography and TLC with pNPG substrate and acarbose positive control. The inhibitory activity testing of the α-glucosidase enzyme was executed using an ELISA reader with a wavelength of 405 nm. The result revealed that the ethyl acetate extract had inhibitory activity of the α-glucosidase enzym with an IC50 of 79,65 ppm. The F-5.5 fraction was the best fraction with an IC50 value of 18,99 ppm. The acarbose activity in inhibiting the α-glucosidase enzyme had an IC50 value of 18,05 ppm. Keywords : α-glucosidase, antidiabetic, Salam leaves, Syzygium polyanthum
Co-Authors . Syamsudin Achmad Fauzi Isa Arini Khaerunnisa Aufa Ali Fajar Nanda Nasukha Danty Nur Alvionitasari Djamil, Ratna Edi Susanto Herson Cahaya Himawan Ika Oktavia Laura Stephanie Joner Lia Rahmania Nico Satria Salim Nuraini Saadah Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Putri Syafalia Ratna Djamil Riska Diana Ristanti Mega Lestari Siti Mariam Syamsoedin Abdilah Syamsudin Syamsudin Verari Vernando Yesi Desmiaty, Yesi