Journal of Health (JoH)
Vol 10 No 2 (2023): Journal of Health (JoH) - July

Karakterisasi dan Formulasi Uji Sitotoksik Nanopartikel PLGA-pcDNA 3.1-SB3-HBcAg dari Gel Retardation Assay sebagai Delivery Agent Kandidat Vaksin DNA Hepatitis B

Lalu Unsunnidhal (Universitas Mataram)
Raudatul Jannah (STIKES Yarsi Mataram)
Fihiruddin (Poltekkes Kemenkes Mataram)
Nurul Inayati (Poltekkes Kemenkes Mataram)
Agus Supinganto (STIKES Yarsi Mataram)

Article Info

Publish Date
05 Jul 2023

Abstract

Indonesia is one of the countries that has the highest prevalence of HBsAg (hepatitis B disease), ranging from 2.5% to 10%, with the highest levels reported in North Sulawesi with 33.0%, Papua with 12.8%, and Pontianak with 9.1%. Strategies to overcome these obstacles include using formulations such as nanoparticles, which are formed by coacervation between polymers and DNA. This study showed that the results of the physicochemical characterization of PLGA-pcDNA 3.1-SB3-HBcAg showed a polydispersity index value of 0.246, a particle size of 925 nm, and a zeta potential value of 2.31 mV. PLGA managed to protect PLGA-pcDNA 3.1-SB3-HBcAg from enzymatic degradation, and the viability percentage of the PLGA-pcDNA 3.1-SB3-HBcAg cytotoxicity test was 98.03%, so that PLGA has good potential as a delivery system for PLGA -pcDNA 3.1-SB3- HBcAg.

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Journal Info
Journal of Health (JoH)
Website

Abbrev

joh

Publisher

Sekolah Tinggi Ilmu Kesehatan Guna Bangsa Yogyakarta

Subject

Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Description

Journal of Health (JoH) (p-ISSN 2355-8857 | e-ISSN 2407-6376) publishes research articles, conceptual articles, reports field studies (See Focus and Scope). The articles of this journal are published every six months, that is on January and July (2 issues per year), and published by the Lembaga ...