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Purification and characterization of recombinant Streptokinase expressed in E.coli from Streptococcus equisimilis with N-terminal methionine Vadla, Pavan Kumar; Tummuru, Murali; Kumar, Dinesh
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Streptokinase is a extracellular enzyme which is extracted from strains of beta Hemolytic streptococcus. The enzyme is a non-protease plasminogen activator that activates plasminogen to plasmin and degrades fibrin clot through its specific lysine binding site which is used in thrombolytic therapy. Purification of streptokinase produced from S.equisimilis in E.coli with N-terminal methionine was carried out in 3 Chromatography purification steps, 1) CM-Sepharose-FF at pH 4.2 followed by concentration and dialysis over night with Tris-HCl pH 8.0. Partially purified dialyzed enzyme sample was loaded on to 2) DEAE-Sepharose-FF column. The Purified fractions of DEAE column were pooled and applied on to Sephadex G-100 column. Enzyme purity was confirmed by SDS-PAGE and RP-HPLC.Its biological activity is determined by specific streptokinase assay and characterised the enzyme by Peptide mapping, MALDI-TOF, Isoelectric-focusing and RP-HPLC. The isoelectric point (pI) of streptokinase is around 4.98.The results of characterization shows that it contains two forms (Isomers) of streptokinase expressed in E. coli which was analyzed by RP-HPLC and chromogenic assay. The variation is formed by isomer-1 in which 85% of Streptokinase expressed without methionine (85000IU/mg) and Isomer-2 in which 15% of streptokinase expressed with methionine (nil activity) in E. coli. This phenomenon shows that the presence and absence of methionine in isomers of streptokinase varying the catalytic activity of the enzyme.

The Physiochemical properties, sensory evaluation and shelf life of corn flour supplemented with Acheta gossypii (cricket) flour Ehoche, Elijah Edache; Oluwafunmi, Akanya; Oluwafunmilola, Adefolalu Folasade
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Most consumed diets are made from corn and are deficient in proteins. Corn flour was made from fermented and dried yellow corn and supplemented with 5 and 10 % proteins of crickets. The diets were analyzed for the following physiochemical properties: swelling index, wettability, bulk density, solubility, and water holding capacity as well as sensory characteristics in a 7 point hedonic scale and the maintaining quality of each diet. Standard methods for physiochemical properties were used, and the microbial assay was also to analyze the diets at refrigerated and non-refrigerated conditions. The swelling index(1.83-2.00 g), gelatinization temperature ( 66.3 -72.3Â°C), wettability (22.0-120 seconds), and bulk density (0.59 - 0.67 mg/mL), significantly increased but solubility (14.1 - 3.00%), and oil-water holding capacity ( 90-65 %) reduced significantly in the cricket supplemented diets over the corn flour ( p< 0.05). The sensory characteristics from both the adult and children scored above average on the 7 points hedonic scale, with a major preference for the 5 % cricket supplemented the diet. It is noteworthy that in the 4 weeks of shelf life experiment, all the diets were found to be devoid of the growth of coliforms, yeast and the only occurrence of the mold is below the harmful threshold except with the 10 % cricket supplemented non-refrigerated diet with the growth of B. subtilis on the 3rd week of the experiment. However, the growth of this bacterium is below a harmful threshold. Proper handling and processing are safest before consumption. Supplementations of corn flour with cricket have been found to improve the nutritional quality of corn and may, therefore, be able to solve the problem of malnutrition.

Erythrocyte sedimentation rate in diabetic and non-diabetic patients of cardiovascular disease Kiyani, Mubin Mustafa; Jahan, Saira; Kiyani, Sania Khawar; Rehman, Hamza; Khan, Lal Gul; Iqbal, Umar
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The pervasiveness of global endemic of type II diabetes, driven by population development, increasing obesity, urbanization, and other additional unidentified factors may be crumpled in the next 20yrs. The purpose of this study was to determine the correlation of ESR in diabetic and non-diabetic patients of cardiovascular diseases. It is a co-relational study conducted at KRL hospital Islamabad from February 2018 to September 2018. The study population compromised a total of 180 cases aged 45 to 50 years were taken, in which 60 cases of CVD with DM, 60 cases of CVD without DM and 60 normal healthy population were taken. All patients and controls gave written informed consent. All subjects went through a general physical examination, and a questionnaire was used to collect the records about demographics and past medical history and existing use of medications. In the presented study, Pearson's Correlation between ESR of healthy and ESR of CVD without DM shows a weak correlation between these two variables i.e., 0.127 with a p-value of 0.503. We also establish that there is a direct intermediate relationship between ESR of healthy and ESR of CVD with DM. High level of ESR in CVD patients might indicate the prognosis of DM. To confirm this further researches and studies must be conducted in this area

The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely Î±, Î², Î³, and Î´. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.

Modification Of Carry-Blair Transport Media For Storage Salmonella typhi Supriatin, Yati; Rahayyu, Muqni
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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The aim of this study was to determine transport media modification as alternative media to replace Carry Blair. One type of transport media that often use to carry faeces specimens suspected to contain Salmonella typhi is Carry-Blair media. Studies have been conducted experimentally by storing Salmonella typhi on alternative transport media with Peptone composition, disodium Phosphate, Sodium chloride, Calcium chloride, which is made using a semi-solid and Carry-Blair as a control. Three variety of storage was done (0 hour,6 hours,9 hours) at a temperature 4â°-8â°C and then Salmonella typhi was inoculated in Salmonella Shigella Agar using spread plate technique incubated during 24 hours at 37â°C, counted the number of colonies by the plate count method using the colony counter. The results of ANOVA could be concluded that modification media could be use as alternative media replace Carry-Blair at 6 hours. Based on regression correlation test was assumed that the Salmonella typhi bacteria still life at less than 11 hours 54 minutes.

Pengaruh Penambahan Bakteri Probiotik yang Dipacu dengan Prebiotik Ubi Jalar Terhadap Penurunan Jumlah Bakteri Shigella dysenteriae Secara In Vitro Rahmi, Safitri Nur; Putra, F.X Saptono; Suyana, Suyana
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Bakteri probiotik adalah mikroba yang bersifat menguntungkan dari golongan Bakteri Asam Laktat (BAL). Antara bakteri yang menguntungkan dan patogen akan terjadi kompetisi. Pertumbuhan Bakteri Asam Laktat (BAL) di usus manusia dapat distimulasi dengan cara memberikan substrat- substrat yang dapat dicerna oleh bakteri tersebut sehingga populasinya meningkat dan melawan bakteri patogen. Substrat- substrat yang dapat digunakan oleh BAL untuk menstimulasi pertumbuhannya dikenal dengan nama prebiotik. Tujuan penelitian untuk mengetahui pengaruh penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap jumlah penurunan bakteri Shigella dysenteriae. Desain penelitian ini adalah Post Test with Control. Dalam desain penelitian ini terdapat kontrol dan kelompok eksperimen. Penghitungan jumlah Bakteri Shigella dysenteriae dengan menggunakan selektif media Mac Conkey Agar. Data didapatkan dari jumlah bakteri Shigella dysenteriae setelah penambahan probiotik Lactobacillus casei. Pengolahan data menggunakan software pengolah data. Uji statistik yang digunakan adalah One Way Anova dengan taraf signifikan 5%. Hasil penelitian yang telah dilakukan didapatkan rerata jumlah penurunan bakteri Shigella dysenteriae pada media Mac Conkey Agar adalah 48.14 x 105 CFU/ml, 45.76 x 105 CFU/ml, 42.10 x 105 CFU/ml, 35.90 x 105 CFU/ml, 32.76 x 105 CFU/ml. Hasil uji Anova One Way diperoleh nilai signifikansi 0.000 (<0.05). Ada pengaruh bermakna penambahan berbagai dosis bakteri probiotik Lactobacillus casei terhadap penurunan jumlah bakteri Shigella dysenteriae.

Pemeriksaan Golongan Darah Sistem ABO Metode Slide dengan Reagen Serum Golongan Darah A, B, O Oktari, Anita; Silvia, Nida Daeninur
Jurnal Teknologi Laboratorium Vol 5 No 2 (2016): 2016 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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The standard examination blood type was performed using a reagent that actually less economical. This research in order to determine whether serum blood type can be used as a reagent blood type. It has been already conducted research antibodies in the serum of blood type A, B, and AB. Each serum would reacted with antigent in the blood, indicated by the agglutination. The research method was experimental by performing blood type with drops of serum in the blood type A, B and AB then saw the grade agglutination. The data obtained were processed statistically using Kruskal Wallis. The result showed serum A, B, and O can be used as a substitute reagents alternative anti â€“ A, anti â€“ B, and anti â€“ AB in determining blood type. Based on analysis, quality clotting produced by serum aglutination is not same with the reagent anti â€“ A, anti â€“ B, and anti â€“ AB in determining blood type.

Pemanfaatan Ekstrak Daun Jambu Biji (Psidium guajava Linn) Sebagai Antibakteri dan Antifungi Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Guava (Psidium guajava Linn) is found throughout Indonesia. The leaves contain tannins that can be used as antibacterial and antifungal. The aims of this research are to determine the antiseptic power of guava leaf as antifungal and antibacterial.This research is pre experiments research with laboratory test to determine the inhibitory power of guava ethanol extract as anti-bacterial and anti-fungal. Guava leaves are old made 70% ethanol extract in LPPT UGM using maceration method. The extract made 3 concentrations ie 25%, 50% and 75%. Each concentration was tested for inhibitory by knowing the diameter of growth barrier to Candida albicans and Staphylococcus aureus. Each concentration is repeated 5 times, resulting in 15 data. The data were analyzed descriptively to illustrate their potential comparisons with chlorhexidine as standard materials. The extract of guava leaf using ethanol 70% in laboratory test resulted in average inhibitory zone diameter as follows: for mushroom C.albicans with extract 25%, 50%, and 75% were 13.4mm, 17.6mm, and 19.4mm. While for S. aureus is 2.2mm, 25.6mm, and 27.2mm. The effect of antifungal power of guava leaf extract (Psidium guajava Linn.) on the growth of Candida albicans fungus is smaller compared to Staphylococcus aureus bacteria

Pengaruh Berbagai Dosis Jus Buah Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Dislipidemia Tia, Hesti Dwi; Sistiyono, Sistiyono; Hendarta, Narendra Yoga
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): 2014 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Consumption of excessive fat in the food that we consume daily can increase cholesterol, LDL cholesterol and triglycerides in the bloodthat is called dyslipidemia. Diet, exercise and drugs are the effort can be done to lower the lipid levels. The herbs have been widely used as an alternative to lower cholesterol levels because the active substances contained antihiperlipid properties. Soursop has many benefits in curing some diseases. Soursop contains an active ingredient that can lower LDLâ€™s cholesterol levels, including vitamin C, fiber and niacin. Therefore, the researchers wanted to do experiment using soursop as an alternative for lowering LDLâ€™s cholesterol levels in dyslipidemia white rat serum. The purpose of this experiment was to determine the effect of various doses of soursop juice to decrease of LDLâ€™s cholesterol levels in dyslipidemia white rat serum. This experiment used pre-test and post-test with control group design. Soursop juice doses used was at 0,9; 1,8 and 2,7 g / 200 g BW of white rat. The data were processed using descriptive and statistics by the Kolmogorov Smirnov Test, One Way ANOVA, Post Hoct Test and linear regression in SPSS 16.0 for Windows. The results of this experiment was the percentage decrease of LDL cholesterol levels in the white rat serum of various doses soursop juice, which is 0,9 g / 200 g BW of 46,19%, a dose of 1,8 g / 200 g BW of 52,30 % and a dose of 2,7 g / 200 g BW of 61,62%. There was the effect of various doses of soursop juice to decrease LDLâ€™s cholesterol levels in dyslipidemia white rat serum. The greater the dose ofsoursop juice given, the greater the reduction in LDLâ€™s cholesterol levels in the serum of dyslipidemia white rat.

Gambaran Kadar Trigliserida (Metode Gpo-Pap) Pada Sampel Serum dan Plasma EDTA Hardisari, Ratih; Koiriyah, Binti
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Hasil Laboratorium yang bermutu harus memiliki ketepatan dan ketelitian tinggi. Pemeriksaan laboratorium sangat diperlukan untuk membantu dalam menegakkan diagnosa suatu penyakit secara akurat menurut ukuran medis. Pemeriksaan kadar trigliserid dapat membantu perubahan pola dan gaya hidup sehat, dapat dihindari mengonsumsi makanan yang mengandung karbohidrat atau kadar gula yang tinggi berisiko terkena penyakit jantung dan stroke akan meningkat seiring dengan tingginya kadar trigliserida seseorang. Pemeriksaan untuk menentukan kadar trigliserid biasanya menggunakan sampel serum dan plasma. Mengetahui hasil pemeriksaan kadar trigliserid dan gambaran perbedaan hasil kadar trigliserid metode GPO-PAP dari sampel serum dan plasma EDTA. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian deskriptif disajikan dalam bentuk tabel untuk mengetahui selisih rata-rata kadar trigliserid sampel serum dan plasma EDTA dan grafik untuk mengetahui yang lebih tinggi. Hasil pemeriksaan kadar trigliserid dengan sampel serum rata â€“ rata 143,16 mg/dl sedangkan pemeriksaan kadar trigliserid dengan sampel plasma rata â€“ rata 142,16 mg/dl. Kesimpulan adalah ada perbedaan hasil pemeriksaan kadar trigliserid metode GPO-PAP antara sampel serum dan plasma EDTA adalah 24%.

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Abstract

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Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA