Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Jurnal Teknologi Laboratorium

Website

Published by Poltekkes Kemenkes Yogyakarta

ISSN : - EISSN : - DOI : -

Core Subject : Science, Education,

Chemistry Other

Arjuna Subject : -

Articles 97 Documents

1 2 3 4 5

Potensi Antibakteri Isolat Actinomycetes terhadap Aktivitas Proteolitik dan Amilolitik Escherichia Coli ATTC 25922 Bahar, Meiskha; Zulfa, Fajriati
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.

Isolasi Candida albicans Dari Swab Mukosa Mulut Penderita Diabetes Melitus Tipe 2 Kadek Sri Jayanti, Ni; Jirna, I Nyoman
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Candida albicans can be pathogen when immunity had decreased and physiological function is impaired, such as in diabetes mellitus type 2. This study aims to isolation Candida albicans that collected from the oral cavity of diabetes mellitus type 2 patients. This research is conducted with a descriptive study by observing the presence of Candida albicans in 30 samples of diabetes mellitus type 2 patients, which grows on Potato Dextrose Agar. The microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours. Based on this research was found 14 (46,7%) patients from 30 patients were positive Candida albicans.

Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG Abror, Yogi Khoirul; Woelansari, Evy Diah; Suhariyadi, Suhariyadi
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.

The Presence of Methanol In Alcoholic Beverages Analyzed Using Qualitative Method Navianti, Diah; Tarmizi, Muhammad Ihsan; Holifah, Sinta Nur
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

An alcoholic beverage contains ethyl alcohol or ethanol (C2H5OH)that is produced from fermentation or distillation of sugars. In many cases, either producers or consumers add ethanol with denaturated alcohol containing methanol (CH3OH) as an additive. Methanol can cause blindness and induce comas, and it is deadly in high doses. This study aimed at investigating the presence of methanol or methyl alcohol in alcoholic beverages sold in Palembang, Indonesia. Seventeen samples collected from small shops and supermarkets were taken by accidental sampling. A chromotropic acid method was used to examine the presence of methanol. The results showed that there were 18% of the samples was positive, and 82% was negative. Based on alcohol content, the research showed that all (100%) samples of group A were negative; 33% of group B was positive, and 33% of group C was positive. The study indicated that methanol was still present in alcoholic drinks sold in markets. The government should inform the society that denatured alcohol contains methanol and, therefore, should not be feasible to consume.

Optimasi Waktu Produksi Metabolit Sekunder dan Skrining Aktivitas Antibakteri Isolat Actinomycetes Rizosfer Tanaman Tin (Ficus carica) Warsi, Warsi; Sulistyani, Nanik
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Some Actinomycetes isolates of a tin plant (Ficus carica L.) have been obtained, namely T24M, T18, T19, T24, T25, T34, T37, T41, and T43. The aim of this study was to optimize the production of secondary metabolites (antibiotics) and screening antibacterial activity against Methicillin-Resistant Staphylococcus aureus (MRSA) from the Actinomycetes isolate of the tin rhizosphere. The study was performed with test an activity of the culture fluid from Actinomycetes isolate against MRSA by the well method. The result of optimization secondary metabolite production showed that the second day was the best incubation time to harvest antibiotics. The results showed that bacterial isolates of T24M produced antibiotics that could inhibit MRSA growth.

Variasi Konsentrasi Alfa Siklodekstrin dan Waktu Sentrifugasi Dalam Preparasi Serum Lipemik Pada Pemeriksaan Glukosa Metode GOD-PAP Izzati, Arfa; Riyani, Ani
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Examination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (Î±-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides Â± 1000 mg/dL, Â± 1500 mg/dL and Â± 2000 mg/dL incubated for 30 minute at 4Â°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4Â°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration Â± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration Â± 1500 mg/dL and Â± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute.

Pengembangan Prekultur Oxgall sebagai Sampel Klinis untuk Deteksi Salmonella typhi dengan Metode Real-time PCR Gunawan, Annisa Pratiwi; Djuminar, Ai; Ernawati, Ernawati; Chaidir, Lidya
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Typhoid fever is a significant public health burden in low-income countries caused by Salmonella enterica serotype typhi (S.typhi). Clinical manifestations of typhoid fever are varied and non-specific, making the diagnosis difficult. Using oxgall for pre-incubation as a selective culture medium before amplification of Real-time PCR (RT-PCR) in wholeblood produces a fast and sensitive diagnostic. The purpose of this study was to know the performance oxgall-precultured Real-time PCR for detection of Salmonella sp.. Prior to the sample process, spike method optimization was performed to find out that the reagents were well used for clinical specimens. In the sample process, blood samples from 30 Widal-positive patients were collected for this study . Venous blood samples from typhoid fever patients were taken on the day of diagnosis; 5 ml for blood culture, and 5 ml for RT-PCR. The bacteria were grown in oxgall 10% (standard microbiological laboratory clinics) and incubated for 6 hours (37Â° C) before bacterial DNA was isolated for RT-PCR detection. The results showed that reagen of RT-PCR is good used for a clinical sample and a blood culture was better than RT-PCR using oxgall (positive blood culture results over 24 hours). This suggests that there is a need for further research on the duration of incubation and oxgall concentrations in RT-PCR and the selection of clinical samples.

Antibiotic Resistant and Plasmid Conjugative Study of Salmonella typhi Haji Saeed Akreyi, Waleed; Younis Yousif, Samira; Assafi, Mahde
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The emergence of multi-drug resistant (MDR) bacteria has endangered the efficacy of antibiotics treatment of pathogenic bacteria worldwide. The aim of this research was to investigate the incidence of Salmonella enterica serovar Typhi in Duhok city, Iraq. Specimens of blood and stool were recruited from 267 patients. S. Typhi isolates were diagnosed depending on morphology, biochemical and serological tests. S. Typhi isolates were tested for their antibiotic resistance. Multi-drug resistant S. Typhi isolates were conjugated with E. coli HB101. The plasmid profile of transconjugants was investigated. 15/267 (5.6%) S. Typhi isolates were identified. Based on their biochemical tests, S. Typhi isolates were categorized into two biotypes (I, 26.66% and II, 73.33%). Four resistance patterns were observed. The resistant pattern to ampicillin and tetracycline was the higher (46.6%). Conjugation experiment showed that all antibiotic markers were transferred from S. Typhi to E. coli HB101 with a conjugation frequency of (0.38Ã—10-5). 13.3% of the S. Typhi isolates were multi-drug-resistant resistant and had two small plasmids. Transconjugants E. coli acquired the resistance from the multi-drug resistant S. Typhi. Antibiotics treatment of the pathogens could be hindered by the constant rise of multi-drug-resistant. Further studies are needed to study the mobile genetic elements and their contribution to antibiotics resistance.

Aktivitas Antibakteri Kulit Buah Karika Dieng terhadap Shigella flexneri dan Escherichia coli Novalina, Dhiah; Sugiyarto, Sugiyarto; Susilowati, Ari
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Karika is an endemic plant of Dieng Plateau. The leaf of Karika has been studied to have antibacterial activity against bacteria that cause diarrhea, while the peels are removed or used as a mixture of animal feed. This study aims to determine the antibacterial activity of Karika peels against bacteria that cause poisoning, i.e Shigella flexneri, and Escherichia coli. The sample fractionated to obtain the fraction of n-hexane and ethyl acetate. The fractions were tested for their antibacterial activity against Shigella flexneri and Escherichia coli by the cup-plate method. The results showed that the ethyl acetate fraction (ethyl acetate 50%) had the highest inhibition on Shigella flexneri and Escherichia coli compared with another concentration. Based on the study can be concluded that the peels of Karikahas antibacterial activity against Shigella flexneri and Escherichia coli.

Potensi Ekstrak Daun Carica Pubescens Sebagai Alternatif Antidiare Bakteri Vibrio cholerae dan Shigella dysentriae Astuti, Tri Dyah; Hadi, Wahid Syamsul
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Acute diarrhea is one of the main causes of morbidity and mortality. A people are starting to choose traditional medicines for alternative therapy. Traditional medicines or herbal medicines are considered safer and do not have side effects such as chemical drugs. The purpose of this study was to determine the anti-diarrhea effect of Carica pubescens leaf extract on the bacteria Vibrio cholerae and Shigella dysentriae. This study was conducted by testing the activity of Vibrio cholerae and Shigella dysentriae bacteria on Carica pubescens leaf extract with a well method, which results can be seen from the formation of inhibitory zones. The data obtained were processed using Two Way ANOVA test statistics. The results showed that the leaves extract of Carica Pubescens concentration of 100% had the best therapeutic effect because it had the greatest inhibitory power on the bacteria Vibrio cholerae and Shigella dysentriae.

Page 1 of 10 | Total Record : 97

1 2 3 4 5

Filter by Year

2014 2021

Filter By Issues

All Issue Vol 10 No 1 (2021): inpress Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19" Vol 9 No 2 (2020): 2020 (2) Vol 9 No 1 (2020): 2020 (1) (inpress) Vol 9 No 1 (2020): 2020 (1) Vol 9 No 2 (2020): (In Progress) Vol 8 No 2 (2019): 2019 (2) Vol 8 No 1 (2019): 2019 (1) Vol 7 No 2 (2018): 2018 (2) Vol 7 No 1 (2018): 2018 (1) Vol 6 No 2 (2017): 2017 (2) Vol 6 No 1 (2017): 2017 (1) Vol 5 No 2 (2016): 2016 (2) Vol 5 No 1 (2016): 2016 (1) Vol 3 No 2 (2014): 2014 (2) Vol 3 No 1 (2014): 2014 (1) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA