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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
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Articles 11 Documents
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Search results for , issue "Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist" : 11 Documents clear
Pembuatan Membran Antiseptik Dari Kombinasi Kitosan Dan Kulit Jeruk Nipis (Citrus aurantiifolia Agnes Ayu Biomi; rahayu artini yuan
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.10870

Abstract

As technology develops, air quality tends to decline. One of the air pollutants that is often encountered is bacteria that infect through the air such as Staphylococcus aureus. As a basic protection tool, a cloth mask with a membrane layer can be an alternative solution. One of the materials that can be used is chitosan and lime peel pectin. Lime peel produces essential oils that are used as stimulant, aromatherapy, and antimicrobial enhancers. This study aims to determine the results of characteristic tests, content analysis through phytochemicals and Staphylococcus aureus. The research method used is descriptive exploratory and experimental. The membrane mask made from chitosan of shrimp skin and lime peel has a characteristic thickness of 00.24 mm with a density of 0.60 - 0.90 g/cm3 with a textured heterogeneous surface. Has a water vapor transmission power of 0.00088 g/day/m2. It takes 27.54 days to completely decompose. Grapefruit peel essential oil contains secondary metabolites in the form of alkaloids, flavonoids, tannins, phenolics and triterpenoids. The membrane mask had Staphylococcus aureus antibacterial activity ranging from 14.05 mm. Keywords: Lime peel (Citrus aurantiifolia), Shrimp Skin Chitosan, Membrane mask, Antibacterial.
Efek Obat Antituberkulosis Fase Intensif dan Konsumsi Purin Terhadap Kadar Asam Urat Pasien Tuberkulosis Mustaming Mustaming
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.7939

Abstract

ABSTRACT A high prevalence of TB requires serious treatment with Anti-Tuberculosis Therapy, but the side effects can gain uric acid levels. This study aimed to analyze the effects of intensive phase therapy and high purine consumption patterns with uric acid levels in TB patients with Anti-Tuberculosis Therapy. This research was an analytic study using a prospective cohort design. The population was all tuberculosis patients who received Anti-Tuberculosis Therapy at several Public Health Centers in Samarinda. The sampling technique used consecutive sampling approach. The respondents were selected according to the inclusion criteria and had signed the informed consent. Furthermore, the venous blood was measured to know the levels of the uric acid pre-test. The measurements of uric acid levels post-test were carried out after undergoing Anti-Tuberculosis Therapy for 2 months and filling out the FFQ questionnaire to determine the patterns of high purine consumption. The collected data was analyzed using a t-paired and a one-way anova test. T-Paired test results exhibited that there were increasing trend in the average uric acid levels after following Anti-Tuberculosis Therapy in an intensive phase of 2.53 mg / dL (39.78%) with p-value <0.001. Besides, the one way anova test results showed that the average difference in uric acid levels in respondents who consumed high purine 4-6 times/week was 3.16 mg / dL and was higher than respondents who never consumed high purine or those who consumed high purine 1-3 times/week with a p-value <0.001. An average increase in uric acid levels in TB patients after undergoing the intensive phase of Anti-Tuberculosis Therapy for 2 months with a pyrazinamide and ethambutol regimen and an increase in uric acid levels was higher compared to respondents who consumed high purine. Keywords: uric acid level, high purine consumption, intensive phase
Uji Efektivitas Asap Cair Batang Bambu (Bambusa sp) sebagai Antiseptik Chintia Agatha Oktavia Pah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.10393

Abstract

Batang bambu (Bambusa sp) biasa digunakan dibidang industri,tetapi batang bambu juga dapat diolah sebagai asap cair karena memiliki senyawa alami yaitu fenol, asam asetat dan karbonil yang dapat digunakan sebagai antimikroba. Penelitian ini bertujuan untuk mengurangi bahan kimia dalam penggunaan antiseptik. Dilakukan uji efeltivitas asap cair batang bambu secara in vitro dan in vivo, secara in vitro dilakukan dengan metode kirby bauer, metode ini terdiri dari difusi cakram untuk bakteri dengan jamur, dan metode sumuran untuk jamur, dengan alkohol 70% sebagai kontrol positif dan akuades sebagai kontrol negatif, konsentrasi asap cair yang digunakan adalah 50%, 75%, dan 100% untuk mengetahui konsentrasi optimum dalam menghambat pertumbuhan S.aureus ATCC 25923, E.coli ATCC 25922, C.albicans ATCC 10231 dan A.flavus ATCC 9643. Sementara itu, uji efektivitas secara in vivo dilakukan dengan mengaplikasikan asap cair batang bambu konsentrasi 100% pada telapak tangan responden. Perhitungan jumlah koloni bakteri dan jamur dilakukan sebelum dan setelah diberi perlakuan. Metode yang digunakan adalah Total Plate Count (TPC). Hasil asap cair batang bambu secara in vitro menunjukkan adanya zona hambat pada konsentrasi optimum 100% dengan nilai rata-rata S.aureus ATCC 25923 (6,83 mm), E.coli ATCC 25922 (3,3 mm) sedangkan pada C.albicans ATCC10231 dan A.flavus ATCC 9643 tidak terdapat zona hambat pada konsentrasi optimum 100%. Hasil secara in vivo menunjukkan asap cair batang bambu konsentrasi 100% efektif dalam menghambat pertumbuhan bakteri sebesar 82,3% dan jamur sebesar 73%. Hasil penelitian in vivo didukung dengan hasil kuesioner kesukaan responden terhadap asap cair batang bambu yaitu diantaranya suka terhadap warna 91,6%, terhadap aroma 75%, suka terhadap asap cair karena tidak menimbulkan efek kekeringan pada tangan sebesar 91,7% dan tidak menimbulkan efek lainnya (seperti sensasi terbakar) 100%.
Efektifitas Nutrisi Hidroponik AB MIX Sebagai Larvasida Alternatif Larva Nyamuk Aedes aegypti Yauwan Tobing Lukiyono; Vella - Rohmayani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.10656

Abstract

ABSTRACT            Hydroponics is a technique for cultivating vegetable and fruit plants. The principle of hydroponic technique is the provision of nutrient solution as a source of plant nutrients. These plants are grown in certain media using water instead of soil. The presence of stagnant water in hydroponic techniques can be a breeding place for Aedes aegypti larvae. Aedes aegypti mosquito can do as a vector for dengue fever. Dengue fever is still a public health problem today. The purpose of this study was to analyze the potential of AB mix as a nutrient for hydroponic plants as larvicides of the Aedes aegypti mosquito.This research was a pure experimental research. The research design used a pre post only group design. The research sample was F3 larvae of Aedes aegypti instar III. The research variables were the mortality of Aedes aegypti larvae and variations in the concentration of AB mix. The test was repeated 10 times. Data were analyzed using one-way ANOVA. Based on the results obtained in all treatments there was death or mortality of mosquito larvae. The administration of 75% AB Mix with a duration of 70 minutes is the most optimal treatment to eradicate Aedes aegypti mosquito larvae. So, it can be concluded that AB Mix can be used as an alternative larvicide to eradicate Aedes aegypti mosquito larvae. Keywords                   : AB Mix, mortalitas dan Larva Aedes aegypti
Daya Hambat Ekstrak Daun Sirih dan Daun Legundi Terhadap Pertumbuhan Bakteri Staphylococcus aureus Ida Bagus Oka Suyasa; Heri Setiyo Bekti; Luh Putu Rinawati; Luh Putu Laksmi; Putu Diah Wahyuni; Desak Gede Dwi Agustini; Aprilia Rakhmawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11015

Abstract

Staphylococcus aureus is one of the causes of infectious disease, ranging from mild to severe infections such as meningitis. The drug that is often used is the penicillin class of antibiotics. Apart from chemical antibiotics, natural antibacterial compunds from plants can be also used as an alternative treatment for this infection. Such as green betel (Piper betle L.) and legundi (Vitex trifolia L.). The ethanol extract of betel leaf has antibacterial activity against Staphylococcus aureus. The phytochemical content in legundi leaf is also able to inhibit the growth of Staphylococcus aureus. The ethanol extract of betel leaf and legundi is made through a maceration process. The extract concentrations used in this study were 20%, 30%, and 40%. The inhibition test of Staphylococcus aureus using the disc method. From the results of this study, the active substances found in betel leaf were flavonoids, tannins, and phenols, while in legundi were found flavonoids, tannins and phenols, and quinones. In total, the mean inhibition zone in this study ranged from 7.87mm to 17.33mm where legundi leaf extract was in the moderate category, betel and combination extract were classified as strong. There was a difference in the diameter of the inhibition zone of betel and legundi extract at a concentration 20% with 40%. In the combination extract there is a difference in the concentration of 20% with 40% and 30% with 40%. An antimicrobial compound can be said to synergize if a mixture of two or more antimicrobial compounds is able to provide an effect of each compounds. In this study, it has seen that the mean of the combination is higher than the mean of legundi leaf extract. Meanwhile, the mean of betel leaf extract is still greater than the combination extract.
Evaluasi Penggunaan Biosaliva Dalam Deteksi Sars-Cov-2 Metode RT-PCR Suryanata Kesuma; Suparno Putera Makkadafi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11280

Abstract

SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection.
Prevalensi Hasil Uji Saring Hbsag Pada Darah Donor Di Unit Tranfusi Darah (UTD) PMI Sampang Madura Rahma Widyastuti; Nur Vita Purwaningsih; Ellies Tunjung Sari Maulidiyanti; Tri Ade Saputro
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.12824

Abstract

The screening blood test is a stage that is carried out by each PMI to ensure the safety before blood transfusion. It is usually done on antibodies (Ab) such as anti-HCV, anti-HIV, TPHA or antigen (Ag) such as HBsAg. HBsAg is the outer coat protein of hepatitis B virus (HBV) and a sign that the individual has been infected with the hepatitis B virus. Hepatitis B virus can cause acute problems and even cirrhosis of the liver. One of the screening blood test methods which is used to detect hepatitis B is ELISA (Enzyme Linked Immuno Sorbent Assay) method. This research was conducted at UTD PMI Sampang by using the data collection in February 2020, and aimed to find out the overview of HBsAg examination results on the screening blood test using ELISA method based on age and sex. Based on the screening blood test, 9 people (3%) were reactive HBsAg and 345 people (97%) were non-reactive. In the age category, the highest number of reactive HBsAg donors was in the age group of 18-24 years old, that was 4 donors (1.1%), while the highest number of non-reactive HBsAg donors was in the age group of 25-44 years old, that was 184 people ( 51.9%). In the gender category, donors with the most reactive HBsAg results were male, that was 8 people (2.3%) and donors with the highest non-reactive HBsAg results was male, that was 283 people (79.9%). In order to minimize hepatitis B, it is expected that society should avoid activities which can cause hepatitis B infection. Keywords        : HBsAg, Blood Transfusion, Screening Blood Test 
Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis puspitasari Puspitasari; Andika Aliviameita
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.12667

Abstract

Sample stability, part of the pre-analytic phase is an important component that can affect clinical laboratory results. Handling of blood samples and storage methods can significantly affect the results of hematological examinations. This study aims to determine the effect of delay time and storage temperature on the results of the examination of leukocyte count, hemoglobin level, erythrocyte count, hematocrit level, and platelet count. The type of research used is a quantitative analysis using laboratory experimental methods. The research was carried out in the hematology laboratory of the Medical Laboratory Technology Study Program, University Muhammadiyah of Sidoarjo in September - December 2021. The results of the Two Way ANOVA test showed that there was no statistically significant effect between the length of time delay and storage temperature on the results of the examination of leukocyte count, hemoglobin level, number of erythrocytes. Friedman test results showed that there was a statistically significant effect between the length of time of delay and storage temperature on hematocrit levels and platelet counts.
Identifikasi dan Enumerasi Escherichia coli dengan Kombinasi Metode MPN-PCR Atik Kurniawati; Dwi Listyorini; Agung Witjoro
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.7592

Abstract

Healthy and nutritious food should be free from biological contaminants. The presence of Escherichia coli in food is an indicator contamination and also presence of other pathogenic bacteria. This study aims to determine the value of MPN E. coli in food samples using the MPN-PCR combination technique. Descriptive research method. Samples were taken from 5 traders in Malang City. The results showed that 3 out of 5 food samples were contaminated with E. coli, indicated by the presence of a specific band amplified and produced a DNA band measuring 584 base pairs in agarose gel electrophoresis. Conclusion: MPN value of E. coli in the three food samples code II 34 MPN / gram, code III 75 MPN / gram and V code 290 MPN / gram. The MPN-PCR combination can be used as an alternative method for the conventional MPN test because it can detect the presence of E. coli bacteria in food quickly and reliably
Description Of Ciprofloxacin Sensitivity On Bacteria Causing UTI In T2DM Patients Muhammad Ibnu Nazari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.6628

Abstract

Diabetes mellitus is a chronic metabolic disease characterized by a state of hyperglycemia due to a lack of insulin production by the pancreas or the body's inability to use the insulin produced or both. Uncontrolled hyperglycemia can suppress the immune system and making the body vulnerable to infection, especially urinary tract infections (UTIs). The main choice in UTI treatment is antibiotic therapy, one of the most frequently used is ciprofloxacin from the fluoroquinolone group.The research design used was descriptive method with prevalence studies. The research was done using antibiotic sensitivity tests with agar diffusion methods. The number of samples were taken using total sampling method.The results of ciprofloxacin sensitivity test were obtained as many as 22 isolates. Escherichia coli bacteria are sensitive to ciprofloxacin at 66.67%, intermediate and resistant 16.67% respectively. Pseudomonas aeruginosa are 60% sensitive and 40% intermediate. Enterobacter aerogenes are 66,67% sensitive and 33,33% intermediate. Shigella spp. are 100% sensitive, and Klebsiella spp. 100% resistant.The conclusion from the study showed that ciprofloxacin are an effective empirical therapy for UTI.

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