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Tropical Animal Science Journal

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Published by Institut Pertanian Bogor

ISSN : 2615787X EISSN : 2615790X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Energy

ropical Animal Science Journal (Trop. Anim. Sci. J.) previously Media Peternakan is a scientific journal covering broad aspects of tropical animal sciences. Started from 2018, the title is changed from Media Peternakan in order to develop and expand the distribution as well as increase the visibility of the journal. The journal is published three times a year in April, August, and December by Faculty of Animal Science, IPB University (Bogor Agricultural University), associated with Animal Scientist's Society of Indonesia. The first edition with the new title will be published in April 2018 edition (Vol 41 No 1 2018), while the previous edition (up to 2017 edition) will still use Media Peternakan as the title and could be accessed in the old website (http://medpet.journal.ipb.ac.id/). This journal has been accredited by National Journal Accreditation (ARJUNA) Managed by Ministry of Research, Technology, and Higher Education, Republic Indonesia with First Grade (Sinta 1) since year 2018 to 2022 according to the decree No. 30/E/KPT/2018.

Arjuna Subject : -

Articles 268 Documents

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Authentication of Sumateran Wild Boar (Sus scrofa vittatus) Meat Contamination by Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) Technique of Cytochrome b Gene M. W. Adiningsih; R. D. Soejoedono; T. Purnawarman; H. Latif; O. N. Poetri; D. D. Putri
Tropical Animal Science Journal Vol. 41 No. 3 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

High population of Sumateran wild boar causes the increasing number of hunting and overflowing of wild boar meat availability in the market at a very low price. This condition leads to the falsification of meat. This study was aimed to characterize and differentiate Sumateran wild boar meat (Sus scrofa vittatus) and beef (Bos taurus) since Sumateran wild boar meat is often falsified as a beef in Indonesian traditional market. The domesticated pork meat (Sus scrofa domestica) was also investigated to compare Sumateran wild boar meat and domestic pork. Samples used in this study were DNA (deoxyribonucleic acid) from pork (Sus scrofa domestica), Sumateran wild boar meat (Sus scrofa vittatus), and beef (Bos taurus). Beef and pork were obtained from traditional markets in Bogor city whereas Sumateran wild boar meat was obtained from Way Kanan Regency, Lampung Province. Herein, we performed a thorough investigation on Sumateran wild boar meat (Sus scrofa vittatus) and beef (Bos taurus) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. This study utilized cytochrome b primer from mitochondria DNA with the size target amplification of 359 bp. This study utilized the restriction endonuclease enzymes, i.e. AluI, BsaJI, HindIII, RsaI, HaeIII, and TaqαI, in order to digest the amplification products from cytochrome b gene. The results showed that HaeIII is the merely specific enzyme to discriminate wild boar meat, domestic pork, and beef since HaeIII was able to digest these three meats in different locations of cyt b gene. However, AluI, BsaJI, HindIII, RsaI, and TaqαI enzymes were not specific enough to differentiate wild boar meat and domestic pork. In conclusion, among six tested enzymes, the use of PCR-RFLP analysis of the cyt b gene followed by digestion using HaeIII restriction enzyme provides a simple, relatively quick, and accurate identification of Sumateran wild boar species.

Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene K. Eriani; A. Azhar; M. Ihdina; B. Rosadi; M. Rizal; A. Boediono
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Sperms are vulnerable to oxidative stress due to the high proportion of polyunsaturated fatty acids within their membranes. This condition could decrease sperms quality during preservation. β-carotene is among antioxidants able to neutralize reactive oxygen species, natural by-products of oxygen metabolism in sperms. This study was done to investigate the capability and protective effect of this antioxidant on motility, viability, acrosomal integrity, and plasma membrane integrity of frozen-thawed sperms of Aceh swamp buffalo. The study was conducted using completely randomized design with four treatments and five replications. Fresh semen was diluted in egg-yolk tris-based extenders with the addition of antioxidant of 0% (w/v) (control, P0), 0.625% (w/v) (P1), 1.25% (w/v) (P2), and 2.5% (w/v) (P3) prior to freezing. Variables of sperms quality examined were motility, viability, acrosomal integrity, and plasma membrane integrity of semen after dilution, equilibration, and thawing. Semen having the best post-thawing quality after β-carotene treatment was used for artificial insemination (AI) for accessing its pregnancy rate. The results showed that the addition of 0.625% (w/v) β-carotene (P1) resulted in the highest motility, viability, acrosomal integrity, and plasma membrane integrity. Pregnancy rate of buffalo inseminated with semen treated with 0.625% β-carotene (P1) was 50%. In conclusion, administration of 0.625% β-carotene was able to maintain and could protect motility, viability, acrosomal integrity, and plasma membrane integrity of frozen sperms of Aceh swamp buffalo that are good for artificial insemination.

Haplotype Diversity of Partial Cytochrome b Gene in Kebumen Ongole Grade Cattle T. Hartatik; D. Maharani; J. H. P. Sidadolog; A. Fathoni; Sumadi Sumadi
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Cytochrome b gene is commonly used to figure out the genetic variation in animals. This research was designed to explore specific markers of Kebumen Ongole grade cattle and to understand the relationship between Kebumen and Brahman cattle using mtDNA cyt b sequences. The sequence of mtDNA cytochrome b gene of 14 individuals Kebumen Ongole grade cattle and 15 individuals Brahman cattle was determined by using polymerase chain reaction and sequencing with a pair of primer. The haploview software was utilized to linkage disequilibrium (LD) analysis. As a result, 36 SNPs (single nucleotide polymorphisms) with 8 haplotypes were identified in Kebumen Ongole grade cattle. In contrast, a small number of SNPs (7) and haplotypes (6) were observed in Brahman cattle. The homozigosity in Brahman cattle was higher than that in Kebumen Ongole grade cattle cattle. Moreover, based on mtDNA sequences, 50% of Kebumen Ongole grade cattle had 85% similarity with Brahman cattle. The SNP number and LD values of Kebumen Ongole grade cattle were greater than those of Brahman cattle. It was presumed that Brahman cattle might be introduced into Kebumen territory. In conclusion, some of Kebumen Ongole grade cattle have genetic relation with Brahman cattle. In addition, the information of SNP and haplotype results from this research can be used as markers to differentiate breeds of animals.

Performance and Intestinal Profiles of Tegal Duck Fed Ration Supplemented with Prebiotics N. Iriyanti; B. Hartoyo; S. Suhermiyati
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was aimed to determine the effects of prebiotics on performance and gastrointestinal profiles of Tegal ducks. One hundred two-month-old male Tegal ducks were distributed into 5 treatment groups; R0= control; R1= FOS; R2= MOS; R3= Inulin; and R4= natural prebiotic (water hyacinth/Eichhornia crassipes). The prebiotics were given at 0.2% of the total feed (w/w). The observed variables were performances and gastrointestinal profiles. This study used a completely randomized design and continued with honestly significant difference test. Performance (live weight, body weight gain, and feed intake) and intestinal profile (percentage weights of intestine, gizzard, crop, and pancreas) of ducks were not significantly affected by prebiotic supplementation in feed. FOS prebiotic and natural prebiotic from water hyacinth showed increased (P<0.05) the percentage of carcass and cecum weight compared to the other treatments. In conclusion, the use of different prebiotics resulted in relatively similar performances and digestive tract profiles except the percentage of carcass and cecum weight that increased by 4.5% and 36.67%, respectively, with the supplementation of either FOS or water hyacinth.

Growth Performance and Ruminal Metabolic Variables of Goats Fed Rain Tree (Samanea saman) Pods M. J. Morais; C. C. Sevilla; J. T. Dizon; G. L. Manulat; E. E. C. Abes; A. A. Angeles
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

The effect of rain tree pods (RTP) or acacia pods on the growth performance, rumen metabolites, and digestibility of dry matter (DM) and crude protein (CP) in goats was evaluated through feeding trial and in situ methods. Eighteen 7 month-old Philippine native goats with body weight range of 7.86±1.28 kg were divided into three groups consisting of six replicates and randomly assigned to one of treatment diets containing 0%, 50%, and 100% RTP in the concentrate portion of a ration containing 65% roughage and 35% concentrate. Three rumen-cannulated mature goats were used in the in situ studies to determine the dietary effects on rumen fluid pH, total volatile fatty acids (VFA), ammonia, and rumen DM and CP digestibilities. Differences (P values < 0.05) were observed on daily gain, total feed intake, and feed efficiency with diets having >50% RTP generally resulted in lower above-stated performance parameters. Rumen fluid pH of goats decreased after feeding and was lowest in goats fed with 50% RTP. Concentrations of VFA and ammonia were not significantly different. Slowly degradable DM of the test diets were higher in concentrate mixture without RTP. Degradable fractions of CP had highest value on concentrate mixture without RTP while lowest on 100% RTP. However, no differences were observed on potentially digestible fractions and degradability constants of DM and CP. It could be concluded that RTPs can be an alternative ingredient in concentrate mixtures given up to 50% in the mixture as part of a daily ration for goats.

The Growth Efficiency and Carcass Characteristics of Dorper Sheep Treated by Corn Inclusion as Energy into Palm Kernel Cake Based-Diet O. A. Saeed; A. Q. Sazili; H. Akit; A. R. Alimon; M. Mazlan; A. A. Samsudin
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study investigated the effect of energy as partial replacement of a diet based on palm kernel cake (PKC) on the growth performance, nutrient intake, digestibility, carcass quality, and economic cost of Dorper sheep. Twenty-seven Dorper rams (initial body weight 15 ± 0.59 kg) were kept in individual pens and divided randomly into dietary groups consisting of T1: basal diet + 0% corn (control), T2: basal diet + 5% corn, and T3: basal diet + 10% corn. Feed intake was recorded on a daily basis. On day 110, 4 lambs from each group were randomly chosen and transferred into metabolism crates to determine nutrient digestibility and completely randomized design was used. The results showed that high feed intake was in T3 while, the DM, CP, NDF, and ADF digestibility were decreased significantly with the increasing level of corn as an energy source. The carcass cuts were not affected by the dietary treatments. Significant differences were noted in the non-carcass parts (heart, lungs and trachea, defattted kidneys, liver, spleen, and kidney fat) with T3 having the highest value. The feeding cost was slightly increased when PKC was replaced by corn. In conclusion, inclusion of 10% corn as a source of energy in 65.3% PKC as fattening diets had effects on growth BW, ADG, and organs performance on Dorper lambs.

Improvement of Indigofera zollingeriana Production and Methionine Content through Inoculation of Nitrogen-Fixing Bacteria P. S. Hutapea; L. Abdullah; P. D. M. H. Karti; I. Anas
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Indigofera zollingeriana is a tremendous potential as a forage plant due to its high protein content. This experiment was conducted to increase production and nutrient quality of I. zollingeriana biomass, especially methionine content through inoculation of nitrogen fixing bacteria. Nine isolates obtained from nodule of I. zollingeriana were screened for 37 days in greenhouse, resulting in three desirable isolates. These three selected isolates at density of 105 cfu/mL and 107 cfu/mL were inoculated as much as 1 mL per seedling. Uninoculated plants were also grown as control treatment, i.e., positive (+ 0.05% KNO3 (w/v)) and negative. The experiment was laid out in a completely randomized design with four replicates and carried out for three months. Plant biomass was analyzed for amino acid and other nutrient contents. Moreover, nodulation activity was also observed by counting and weighting the nodules. The results showed that Bradyrhizobium sp. P8 828, Bradyrhizobium sp. PZS_A08, and Roseomonas sp. CMS4Y-2-2 significantly increased shoot production. Subsequent experiment showed that plant inoculation with Bradyrhizobium sp. PZS_A08 at 105 cfu/mL significantly increased dry matter content. Inoculation with Bradyrhizobium sp. PZS_A08 and Roseomonas sp. CMS4Y-2-2 at 107 cfu/mL produced higher crude protein contents that were better than the application of nitrogen fertilizer. Inoculation treatment with Bradyrhizobium sp. P8 828 at 105 cfu/mL was the most effective in improving methionine content. It is concluded that production and quality of biomass obtained from inoculation treatments were comparable to the application of inorganic N-fertilizer.

Growth Performances and Carcass Characteristics of Broiler Chickens Fed Akasya [Samanea Saman (Jacq.) Merr.] Pod Meal F. C. C. Reyes; A. T. A. Aguirre; E. M. Agbisit Jr; F. E. Merca; G. L. Manulat; A. A. Angeles
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Akasya pods are seasonally abundant and are good sources of protein and energy and have been considered as an alternative feed source for livestock. This study was conducted to determine the effects of feeding Akasya pod meal (APM) on the growth performances and carcass characteristics of broiler chickens. Three hundred straight-run 7-day-old Cobb broiler chicks were randomly allocated to five dietary treatments containing 0%, 0.5%, 1.0%, 2.5%, and 5.0% APM following a completely randomized design. Each treatment was replicated 10 times with 10 birds per replicate. The digestibility of nutrients was also evaluated to determine the effect of different levels of APM on the efficiency of nutrient digestion in broilers. Broilers fed APM level at more than 1.0% had lower body weights and body weight gains, but feed intake, feed conversion ratio, livability, and carcass characteristics were not affected by increasing levels of APM. The digestibility of crude protein (CP), crude fiber (CF), nitrogen free extract (NFE), ether extract (EE), and energy were the same in broilers across treatments. Increased income over chick and feed cost (IOFCC) was generated from broilers fed diets with 0.5% APM, but progressively declined as APM level in the diet was increased. In conclusion, APM can be used in broiler diets at inclusion levels up to 1.0% wherein growth performance and carcass characteristics were optimum in broilers and income generated was improved at 0.5% level.

Purification of Bacteriocin from Lactobacillus plantarum IIA-1A5 Grown in Various Whey Cheese Media Under Freeze Dried Condition R. Fatmarani; I. I. Arief; C. Budiman
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Lactobacillus plantarum IIA-1A5 is a lactic acid bacteria (LAB) that has been reported to have capacity to produce bacteriocin, usually called plantaricin. This bacteriocin is usually produced in commercial synthetic media. However, the media is expensive, thus finding a novel source which is less expensive and abundant is necessary. The present work was aimed to compare the use of gouda and mozarella cheese whey as growth media of L. plantarum IIA-1A5 producing plantaricin, as well as to evaluate the effectiveness of freeze dried plantaricin as an antimicrobial agent. The results showed that gouda and mozarella cheese whey were applicable for growth media of L. plantarum IIA-1A5 and production of plantaricin. The plantaricin produced from the whey showed a size of about 9.6 kDa. Freeze dried plantaricin was shown to be relatively stable in the second week of storage, but there was a slight decrease in protein concentration during storage, indicating that the protein was partially denatured and precipitated. However, the freeze dried plantaricin showed inhibitory activities against S. aureus ATCC 25923, B. cereus ATCC 21332, S. thpymurium ATCC 14028, E. coli ATCC 25922, and P. aeruginosa ATCC 27853 during storage at room temperature.

Milk Lipid and Protein Profiles of Abkhazian and Kackar Goats H. Iskender; O. Kaynar; A. Hayirli; Y. Camadan
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Fat and protein profiles of milk of Abkhazian and Kackar goats, Caucasian breeds, were compared in this study. The milk samples (n= 60) from 60 Abkhazian and Kackar goats were subjected to assessments of lipid profile using the high performance thin layer chromatography and protein profile using the sodium dodecyl sulphate polyacrylamide gel electrophoresis. The milk lipid and protein contents as well as their fractions were compared using student t-test. Total lipid content was 4.23±0.022 g/dl and 3.44±0.026 g/dl for Abkhazian and Kackar goat milk (P<0.0001). Milk triacylglycerol, free fatty acid and diacylglycerol fractions were different (P<0.05), but the cholesterol fraction was similar. Total protein content was 3.94 g/dl and 3.75 g/dl for Abkhazian and Kackar goat milk (P<0.007). The milk fat globule membrane protein mucine1 and xhantine oxidase, α-lactalbumin, α-casein, and κ-casein fractions were different (P<0.05). In conclusion, milk lipid and protein profile differs between Abkhazian and Kackar goats despite living in the same ecosystem. Differences in milk lipid and protein profile could be pertinent to human nutrition and health.

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Faculty of Animal Science Building, IPB University (Bogor Agricultural University) Jln Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Prof. Dr. Ir. Komang G. Wiryawan

Contact Email kgwiryawan@yahoo.com

Phone +622518421692

Journal Mail Official mediapeternakan@apps.ipb.ac.id

Editorial Address Faculty of Animal Science Building, IPB University (Bogor Agricultural University) Jln Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia

Location Kota bogor, Jawa barat INDONESIA

Abstract

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Abstract

Abstract

Abstract

Contact Name
Prof. Dr. Ir. Komang G. Wiryawan

Contact Email
kgwiryawan@yahoo.com

Phone
+622518421692

Journal Mail Official
mediapeternakan@apps.ipb.ac.id

Editorial Address
Faculty of Animal Science Building, IPB University (Bogor Agricultural University) Jln Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia

Location
Kota bogor,

Jawa barat

INDONESIA