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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Back Cover AB Vol 21 No 2 (2017) Rahmawati, Syamsidah
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.587 KB) | DOI: 10.14203/ab.v21i2.321

Abstract

Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Kanti, Atit
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.036 KB) | DOI: 10.14203/ab.v19i2.252

Abstract

The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30°C, and initial moisture content was adjusted to 60%. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, and amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.
Identification of nifD and nifH Genes of Methanotrophic Bacteria from Rice Field Bintarti, Ari Fina; Rusmana, Iman; Wahyudi, Aris Tri
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (879.425 KB) | DOI: 10.1234/97

Abstract

Metanotrophic bacteria have ability to oxidize methane and fix atmospheric nitrogen, hence the bacteria has an important role as a nitrogen source provider on wetland area like rice fields. Nitrogen fixation process is catalyzed by the nitrogenase enzyme complex, encoded by nifD and nifH genes. However, characteristic of these genes from indigenous-methanotrophic bacteria still poorly understood. Hence, nifD and nifH genes of methanotrophic bacteria isolated from rice fields in Indonesia (BGM3, BGM9, SS1, SS3, SS10, ST18, SP3 and INP4) were identified and characterized. Detection of nifH and nifD genes was conducted by polymerase chain reaction (PCR) amplification. nifH and nifD gene sequences were analyzed using BLAST-X and phylogenetic trees were constructed using Neighbour Joining method. Based on nifH sequences analysis, SS1 closely related to Beijerinckia mobilis and SS3, SS10, ST 18 closely related to Beijerinckia indica subsp. indica ATCC 9039, while, BGM3, INP4, and BGM9 related to nifH of uncultured nitrogen-fixing bacterium. In other hand, sequence analysis of nifD gene showed that SS1, SS3, SS10, ST 18 closely related to B. indica subsp. indica ATCC 9039 and BGM3, BGM9, INP4 closely related to Xanthobacter autotrophicus Py2. Identification by 16 SrRNA indicated that SS1, SS3, SS10, and ST18 had closeness to Beijerinckia sp. P310-1, while INP4 closely related to Xanthobacter sp. M5C24.
Tissue Culture Propagation of “Hausa potato” (Solenostemon rotundifolius (Poir) JK Morton) Witjaksono, Witjaksono; Leksonowati, Aryani
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/38

Abstract

We have developed an effective plant propagation method of “hausa potato‟ through shoot proliferation  in vitro, acclimatization in the greenhouse and transplantation of the plantlet on the individual media container for planting material production. The shoot cultures were initiated from shoots that grew from tuber collected from the field, disinfested using standard method and transferred to MS medium with 1 mg/l  of  BA for shoot proliferation. The proliferating shoots were then subcultured to MS medium with no growth regulators to induce rooting and enlarge the shoot. The plantlets were acclimatized in a plastic box containing medium of a mixture of cocopeat, sands, rice husk charcoal and top soil covered with plastic film to maintain humidity. The plantlets survived at 85% and almost 100% grew into planting materials ready for transplanting in the soil.  The planting materials survived in the field and produced tuber normally. Keywords: Solanostemon rotundifolius, hausa potato, kentang hitam, in vitro propagation, acclimatization, planting materials.
Back Cover AB Vol 20 No 1 (2016) Anugerah, Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (985.742 KB) | DOI: 10.14203/ab.v20i1.264

Abstract

Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Thontowi, Ahmad; Rahmani, Nanik; Yopi, Yopi
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/75

Abstract

Bt05 is marine bacterium which was isolated from the Jakarta Bay, Indonesia. The aim of this study was to characterize PAHs-degrading property, molecular identification by partial analysis of 16S rRNA gene and to partially analyze dioxygenase gene of Bt05 isolate. Our further study on this isolate revealed that it could degrade three PAHs (phenanthrene, dibenzothiophene, fluorene) between 60%–90% within 11 days at 100 ppm level. This finding indicated the potential of the isolate for bioremediation of PAHs. The isolate was identified as Alteromonas alvinellae by phylogenetic analysis of 16S rRNA gene sequence. Sequence analysis of the PCR product of PAH dioxygenase genes amplified using two primer set (iiDA and ppAH) of the isolate were identified 97% as naphthalene dioxygenase gene (phaAc) and 58% as 1,2-dioxygenase.
Construction of Binary Vector With Wound Inducible Promoter for Hbsag Expression: Development of Plant-Based Edible Hepatitis B Vaccine from Indonesian Isolate Suhandono, Sony; Rachman, Ernawati A.Giri; Zainuddin, Ima M.; Utari, Putri Dwi; Supraba, Apsari; Estiati, Amy
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/22

Abstract

Hepatitis B is a serious infectious disease In the third world countries including Indonesia . Vaccination is  the most effective way to prevent the spread of the disease;  therefore the demand for HBV vaccine is high.  In order to produce more vaccine at lower cost, transgenic plant can be chosen to express the vaccine with the above criteria. Several researches were successfully producing transgenic plants expressing HBsAg that formed virus-like particles and  induced  immune response  in  human.  However. HBsAg expression  in  transgenic plant needs to be  improved especially on gene  expression control system. Here, we describe the construction of HBsAg .  structural gene under the control of wound  inducible promoter, MeEFl promoter from  manihot esculenta Crantz. The HBsAg gene was amplified using PCR from HBV genome isolated from an  Indonesian patient. The gene was subsequently fused with VSPaS signal peptide, which targeted the reticulum endoplasm of plant cell . The construct was cloned into binary expression vector for Agrobacterium plant Transformation  in  near future.Keywords: HBsAg. VSPaS signal peptide and MeEFI promoter
Editors Preface AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.21 KB) | DOI: 10.14203/ab.v21i1.305

Abstract

A Highly Abundant Lectin Protein in Arabidopsis thaliana Confers Resistance Against Pathogens Omega, Maria prihtamala; Hall, Skye Thomas; Schenk, Peer; Kobe, Bostjan
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/58

Abstract

Lectins are glycoproteins that recognize and bind  to specific carbohydrates. they are  involved  in a range of biological  functions,  such  as  plant defence,  storage  proteins  seed  germination  and  plant  microbe  interactions. Lectin 3.1  (At3g 15356) is a protein in plant model , Arabidopsis thaliana, that has been shown  to be up-regulated in  all  defence  pathways, especially  in  responce  to methyl  ester jasmonate  (MJ) . All  thal was  known  about  the gene  was  that  it  had  good  homology  to  the  beta domain  of legume  lectins. That aim of  this  project was  to characterize  the  structure  and  function  of  the  Iectin  protein  using  CD spectra and  X-ray  crystallography. A  T­DNA  insertion  line  for the  lectin  gene  and  a number  of 35S  over-expression  lines  that  had  varying  levels  of expression had been generated, but none of these showed any obvious  phenotype. Two protein bands were observed on  Comassie stained SDS-PAGE gels  in  the over-expression  lines and  in MJ  induced wild-type  (WT) . The  two protein bands represented  two  isoforms of  the  lectin 3.1  protein ; in  a glycsylation assay the  larger protein  band was shown  to be heavily glycosylated . A nematode (M. incognita) disease assay djscovered  that the  lectin over-expression lines  had  less  nematode  eggs  compared  to  that  of the WT and  that  the  insertion  line  had  more nematode  eggs  than  the WT. This  data  provides  evidence  that   lectin  3. 1  improves. plant  resistance  against M. incognita infection.  interestingly,  the nematode gut  lining  contains  fucose with  which  Iectin  3.1 binds  to. Keywords: Arabidopsis  thaliana,  lectin’s structure and  function,  pathogen resistance
Investigation of Functional Difference between TecIII and TecIV in Mammals Cos-1 Cells Using GFP Fusion Proteins Atmosukarto, Ines; Booker, Grant W.
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2981.061 KB) | DOI: 10.1234/12

Abstract

Signal transduction cascades are critical components of intra- and inter-cellular communication. Key component of such cascades includes tyrosine kinases. One such family of tyrosine kinases is the Tec family of tyrosine kinases . The family of tyrosine kinases is expressed mainly in cells of the hematopoietic lineage, and mutations in at least one of its one member of this family, Btk, has so far been asociated with the human immunodeficiency disorder X-Linked Agammaglobulinemia. Two major isoforms of the Tee transcript, referred to as TecIII and TecIV has been detected in various mouse embryonic and adult tissues, as well as in a number of different hematopoietic cell lines: Tec IV is the full length Tec protein with functional PH, TH, SH3 , SH2 and Kinase domains, while TecIII is generated by the splicing out of exon 8 sequences to yield a shorter peptide with a non-functional SH3 domain. Using GFP-TecIII fusion proteins, this shorter isoform ofTec was shown to have biological characteristics that differed from TECIV.  

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