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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 10 Documents

Search results for , issue "Vol 10, No 2 (2015): (December 2015)" : 10 Documents clear

THE USE OF PATH ANALYSIS IN THE DETERMINATION OF ENVIRONMENTAL FACTOR EFFECTS ON THE TOTAL PRODUCTION OF AQUACULTURE PONDS IN PASURUAN, EAST JAVA PROVINCE Andi Indra Jaya Asaad; Erna Ratnawati; Akhmad Mustafa
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Environmental factors in the form of soil and water quality are the important factors of aquaculture pond productivity, including total production (tiger shrimps, Penaeus monodon, and milkfish, Chanos chanos) in Pasuruan, East Java Province. The objective of this study was to analyze the direct or indirect effects of soil and water quality on the total production of ponds in Pasuruan using a path analysis application. Data were collected in the pond areas around Pasuruan Regency including Nguling, Lekok, Rejoso, Keraton, and Bangil Sub-Districts as well as Pasuruan City. Soil quality was determined as a free variable and exogen; water quality as mediate variable, suspended, and endogen; as well as milkfish production as suspended variable and endogen. Environmental characteristics were illustrated using descriptive statistics, while environment factor effects on total production were analyzed using path analysis. The results of path analysis show that from the 12 analyzed soil quality variables, only two variables were affected in the total production of pond (tiger shrimps and milkfish) namely: contents of soil organic carbon and soil phosphate. While based on 11 water quality variables, two variables (water salinity and water iron) were affected the total production of ponds in Pasuruan Regency. The direct effects of soil organic carbon and phosphate on the total production were 0.314 and -0.600, respectively. Water salinity and water iron gave direct effects on total production amounting to -0.678 and 0.358 respectively. It is also found that two soil variables which were affected in the total production, did not indicate the effect towards water quality in ponds. Further implication of this research is put more attention for these variables into pond’s management in order to gain more production. Technical application could be appropriate for pond preparation and frequently water changing during grow out.

IMPROVED PRODUCTION OF TIGER SHRIMP (Penaeus monodon) THROUGH PROBIOTICS APPLICATION Irsyaphiani Insan; Endhay Kusnendar Kontara; Raden Roro Sri Pudji Sinarni Dewi
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The study was carried out in Brebes District, the North coast of Java. Tiger shrimp farming in Indonesia, particularly in this area faced some problems which caused by improper pond preparation, disease, and low seed quality. Probiotic was applied in pond to solve this problem. The aim of this study was to evaluate the production of tiger shrimp in ponds with probiotic applications. Six experimental ponds (each measuring 0.5 ha) were selected of which three were probiotic ponds and three were controlled. Tiger shrimp postlarvae (PL-30) were stocked at density of four shrimps/m2. Tiger shrimps were reared for three months. Shrimps were fed by commercial pellet. In the first month, shrimp were fed about 7%-5% of the total biomass; in the second months, 3.5%-3% of the total biomass; and in the third month, 2.5%-2% of the total biomass. The treatments in this study were the application of probiotics with concentration of 3 mg/L that were given every five days and control (without probiotics). The results showed the rearing period was 92 ± 6 days in probiotic ponds and 76 ± 16 days in controlled pond. The shrimp in controlled pond should be harvest earlier caused by the high mortality. The average final weight was 16.2 ± 0.7 g in probiotic pond and 15.6 ± 1.9 g in controlled pond. The survival rate was 64.13 ± 12.63% in probiotic pond and 44.17 ± 14.15% in controlled pond. Production was 208 ± 46 kg/pond/cycle in probiotic pond and 123 ± 6 kg/pond/cycle in controlled pond. The result showed that probiotic plays an important role in maintaining water quality parameters and health management as well as increases the survival of shrimp.

IMMUNOGENICITY ASSESSMENT FOR DETERMINATION OF THE MOST POTENTIAL ISOLATES OF KOIHERPES VIRUS Lila Gardenia; Isti Koesharyani; Tatik Mufidah
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.10.2.2015.155-162

Common carp and koi (Cyprinus carpio) are the main consumption fish commodity and ornamental fish in Indonesia. Diseases due to koiherpes virus (KHV) infection had caused a huge loss in both common carp and koi culture industries world-wide. This study was generated to select the most potential candidate from out of three koiherpes virus isolates based on their serological performances for vaccine development to control Koiherpes virus disease in koi. Virus collection, isolation, and propagation in cell culture have been conducted from common and koi samples originated from Banjarmasin, South Kalimantan; Takalar, South Sulawesi; Cirata Lake-Cianjur, West Java; Depok, West Java; Ciseeng, Bogor, West Java; and Purwokerto, Central Java. Determination of the causative agent of the disease was done by PCR (Polymerase Chain Reaction) assay and inoculation of filtrate homogenates from infected fish into cell culture. There are three koiherpes virus isolates (BJMN-1, BJMN-2, and SKBM) which are being successfully isolated, cultured and propagated in Koi Fin (KF-1) cell line. Calculation of tissue culture infection dose from each isolates were 104.55 TCID50/mL, 104.72 TCID50/mL, and 103.28 TCID50/mL. Immunoassay of the three isolates was conducted by Indirect-ELISA method. Polyclonal antibody was made by injecting each isolate to the experiment animals (rat/Rattus norwegicus strain Sprague Dawley). It was shown that all of three isolates have high level of immunogenicity, as seen from the absorbance values at 490 nm wavelength. BJMN-1 isolates have a higher potential for it’s ability to cross-react with the other isolates and provided the highest absorbance values. In future, all isolates can be used as KHV vaccine candidates to prevent KHV infection.

MORPHOLOGICAL CHARACTERIZATION OF THE AFRICAN CATFISH (Clarias gariepinus BURCHELL, 1822) STRAINS INTRODUCED TO INDONESIA Bambang Iswanto; Imron Imron; Rommy Suprapto; Huria Marnis
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

African catfish (Clarias gariepinus Burchell, 1822) has become a great important species in Indonesian aquaculture. Several strains of the African catfish have been introduced to Indonesia for aquaculture purposes, initiated by Dumbo strain from Taiwan in 1985, followed by Paiton strain from Thailand in 1998, then Egypt strain from Egypt in 2007, Masamo strain from Thailand in 2010 and later Kenya strain from Kenya in 2011. Since its introductions, there were no reports yet on their characterization studies. The present study was conducted to morphologically characterize the strains of African catfish introduced to Indonesia, i.e. Dumbo, Paiton, Egypt, Masamo, and Kenya strains. Morphometric and meristic data obtained were analyzed using Principal Component Analysis. Results of the morphometric characterization in the present study revealed that Dumbo, Paiton, Masamo, and Kenya strains were indistinguishable, while morphometric characteristic of Egypt strain was more or less different from those of the other strains. On the other hand, results of the meristic characterization suggested that meristic characteristics of all strains of the introduced African catfish were not different from each other. Therefore, to keep the genetic purity of those introduced strains, they should be properly maintained in isolated places.

IDENTIFICATION OF A LOCAL PROBIOTIC BACTERIUM USING 16S rRNA GENE SEQUENCE THAT WAS USED FOR FIELD TRIAL TO ENHANCED WHITELEG SHRIMP (Litopenaeus vannamei) SURVIVAL Tb. Haeru Rahayu; Ketut Sugama
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The use of local probiotics in the culture of aquatic organisms is increasing with the demand for more environmental-friendly aquaculture practices. The local bacterium isolate considered as a probiotic was added into the water of whiteleg shrimp (Litopenaeus vannamei) culture in a field trial. Four rectangular plastic ponds (ca. 20 m x 30 m per pond) were used for 100 days experimentation for six consecutive crops in two years experiment. Survival, harvest size, feed conversion ratio (FCR) and Vibrio bacterial count was compared with those of shrimp receiving and none of local isolate. Identification based on 16S rRNA gene sequence shown those isolate was Bacillus pumilus strain DURCK14 with 99% homology. Water shrimp pond added a local isolate had significantly higher survival at about 10.0% to 11.7% than shrimp without added the isolate (p<0.05), and better FCR, but no significant different in shrimp harvest size. Vibrio bacterial was undetected by total plate count. Moreover, it shown better projected yields on an annual basis (three crops per year).

EFFECTS OF SALINITY ON OXYGEN CONSUMPTION AND BLOOD PROPERTIES OF YOUNG GREY MULLETS Mugil cephalus Vitas Atmadi Prakoso; Ki Tae Kim; Byung Hwa Min; Rudhy Gustiano; Young Jin Chang
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Oxygen consumption (OC) is one of important factors in aquaculture activities, as the oxygen is a vital condition for all the organisms living in the water and having an aerobic type of respiration. OC is the preferred method for measuring and reporting the metabolic rate in fish. The aims of this study were to evaluate the effects of salinity on OC and blood properties of grey mullets. Five experimental groups were conducted to measure OC and blood properties of grey mullets Mugil cephalus (BW: 187.9 ± 45.8 g) according to salinity (30→0 psu, 0→30 psu) changes; SDS: fish reared in seawater (SW, 30 psu) directly shifted to SW, SGF: SW fish gradually shifted to freshwater (FW, 0 psu), SDF: SW fish directly shifted to FW, FDF: FW fish directly shifted to FW, and FDS: FW fish directly shifted to SW. The result showed that OC tended to decrease in the groups of SW fish shifted to FW showing 194.5 mg O2/kg/h at 25°C in SDS to 82.4 mg O2/kg/h at 15°C in SGF. On the contrary, OC increased in the groups of FW fish shifted to SW showing 80.5 mg O2/kg/h at 15°C in FDF to 184.0 mg O2/kg/h at 25°C in FDS. Cortisol levels at the end of experiments were rapidly increased with the lowering salinities in SW fish shifted to FW showing 20.6 ng/mL in SDS to 316.2 ng/mL in SDF, while those were decreased with the increasing salinities in FW fish shifted to SW showing 40.2 ng/mL in FDF to 10.3 ng/mL in FDS. However, glucose levels showed no significant differences among all experimental groups. Based on the information from this study, aquaculture of grey mullet might be applied or developed in freshwater due to its osmotic adaptation ability.

THE DYNAMICS OF GENETIC VARIABILITY IN THREE GENERATIONS OF MASS SELECTION FOR FAST GROWTH IN AFRICAN CATFISH, Clarias gariepinus ASSESSED BY MICROSATELLITE MARKERS Imron Imron; Bambang Iswanto; Huria Marnis; Rommy Suprapto; Narita Syawalia Ridzwan
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Selective breeding aiming at improving the performance of economically important traits acts by exploiting population’s phenotypic variance. Due to the relationship between phenotype and genotype, selection on phenotype may also affect the profile of genotype. This study was aimed to monitor the impact of three generations of mass selection for fast growth in African catfish, Clarias gariepinus, on genetic variability, assessed by microsatellite. A total of 350 fish representing four populations, namely a composite base population (G-0), selected lines of the first generation (G-1) to the third generation (G-3), were sampled. The samples were screened for their genetic diversity using five microsatellite loci1 namely cga01, cga02, cga03, cga05, and cga09. Several genetic parameters including number of allele (A), allelic richness (AR), observed (Ho) and expected (He) heterozygosity, and fixation index (Fis) were evaluated. The results showed that there was a slight increase in the value of diversity indices in the G-1 relative to the G-0 and to the other two generations. Among these parameters, the number of allele seemed to be the most sensitive parameter in detecting genetic changes. All populations experienced heterozygote deficit and positive fixation index indicating the phenomena of inbreeding. Overall, selection for growth for three generations in African catfish breeding program resulted in significant genetic differentiation between populations. Further, the level of genetic differentiation seemed to accumulate along with the number of generaton in breeding program. However, selection did not result in a decline in genetic diversity within population. A relatively short period of the program, along with the use a high number of broodstock (mating pairs) to produce each generation seems to be able to maintain the stability of genetic diversity of the population.

THE EFFECTS OF CRUDE RECOMBINANT VIRAL PROTEIN VACCINES AGAINST GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) ON HUMPBACK GROUPER (Cromileptes altivelis) Ketut Mahardika; Indah Mastuti
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Infection of Megalocytivirus cause serious mass mortality in marine fish in South East Asian countries. The aim of this study was to produce recombinant of GSDIV capsid protein and its protection to humpback grouper Cromileptes altivelis against grouper sleepy disease iridovirus (GSDIV). A major capsid protein (MCP) was selected for use as a crude subunit vaccines. This gene target (MCP) was inserted to the protein expression system vector of pET SUMO and cloned in cells bacteria Escherichia coli strain BL-21. The MCP was succeded to be induced using 1 mM of IPTG. Results of protein analysis using MALDI TOF-TOF indicated that the MCP has measurement of 49.566 kDa with PI index of 6.00, and contained 453 amino acids. BLAST homology analysis exhibited that the amino acid sequence of the MCP showed high similarity with MCP of Red Sea Bream Iridovirus (RSIV). E. coli expressing MCP protein was inactivated using 0.03% formalin overnight and washed using PBS. The inactivated E. coli as a crude subunit vaccine was then injected intramuscularly to humpback grouper juveniles. Subsequently, the juveniles were challenged tested with GSDIV. The juveniles vaccinated with the MCP recombinant bacteria showed significantly higher survival rates than control those vaccinated with PBS. Thus, the MCP fusion protein is considered as a potential vaccine against GSDIV infections in grouper.

GROWTH RESPONSE OF CLOWN LOACH (Chromobotia macracanthus Bleeker 1852) JUVENILES IMMERSED IN WATER CONTAINING RECOMBINANT GROWTH HORMONE Asep Permana; Alimuddin Alimuddin; Wartono Hadie; Agus Priyadi
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The main problem in the culture of clown loach (Chromobotia macracanthus) is the slow growth rate, which takes about six months to reach its market size (two inches total body length). Slow growth eventually cause a long production time and increase the production costs. An alternative solution can be proposed in order to enhance the growth is by using recombinant growth hormone. The aim of this study was to determine the immersion dose of recombinant Epinephelus lanceolatus growth hormone (rElGH) which can generate the highest growth in clown loach. Larvae at seven day after hatching were hyperosmotic treated with NaCl 2.0% for one minute, then immersed for one hour in water containing 0.3% NaCl, 0.01% bovine serum albumin (BSA), and different doses of rElGH, namely: 0.12 (treatment A), 1.2 (B), 12 (C), and 120 mg/L (D). As control, fish were immersed in water without rElGH and NaCl (control-1), water containing 0.3% NaCl and 0.01% BSA (control-2), and 0.3% NaCl water (control-3). Each treatment was replicated three times. The results showed that clown loach juveniles in treatment B, C, and D had longer total body length (P<0.05) than control-1, while fish treatment A was the same as controls. Survival and body weight were similar in all treatments and controls (P>0.05). In addition, the percentage of large size juveniles increased approximately 5% in treatment B, almost the same as in the medium size, while the small size were decrease compared to the control-1. Thus, the best immersion dose of rElGH was 1.2 mg/L water.

GROWTH AND COLOUR PERFORMANCE OF THE CROSSBREED MARBLE STRAIN Betta splendens AND Betta imbellis Eni Kusrini; Riani Rahmawati; Siti Murniasih; Ruby Vidia Kusumah; Anjang Bangun Prasetio
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Betta ornamental fish of marble strain is a strain which is prefered by the hobbyists, because of its unique color, expensive price, and difficult to obtain the fish color strains. Therefore strain marble betta fish is still dominant for export quality for national and international contests. The aim of this study was to observe the growth and color performance of the crossedbreed marble strains of wild betta, Betta imbellis crossed with Betta splendens. The hybrids of B. imbellis (f) x B. splendens (m) (D) had a specific length and weight rate, (1,113 ± 0.04%/day; 2,531 ± 0.14%/day; 26.61 ± 2.02 mm) which was better than that of the hybrids B. imbellis (m) x B. splendens (f) (C) (1,099 ± 0.02%/day; 2,244 ± 0.13%/day; 25.97 ± 1.33 mm; and 0.0027 ± 0.0003 mm). However, the survival rate of the C hybrids (42.19 ± 11.42%) was higher than those of D (33.67%-17.08%). Based on the obtained results of the color identification the hybrids had as many as 15 characters of color, homozygous strains of marble which became the target of as much as 1%.

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Contact Name
Ofan Bosman

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Ofan Bosman

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Journal Mail Official
iaj.puslitbangkan@gmail.com

Editorial Address
-

Location
Kota adm. jakarta pusat,

Dki jakarta

INDONESIA