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Gilang Nugraha
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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 8 Documents
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Search results for , issue "Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare" : 8 Documents clear
Neutrophil Lymphocyte Ratio (NLR) in COVID-19 Patients Receiving Convalescent Plasma Therapy Desyani Ariza; Andi Maya Kesrianti; Tazya Anggraini Ruslan
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2460

Abstract

Recently, a new RNA virus from the Coronaviridae family was discovered, known as SARS-Cov-2. This virus causes pneumonia and inflammation in the body. One of the laboratory tests used to see inflammation in the body is the Neutrophil Lymphocyte Ratio, or often abbreviated as NLR. NLR is one of the markers of inflammation that can be used simply, efficiently, and reliably because of its high stability and sensitivity. Higher NLR values tend to lead to a severe and poor prognosis, so this test can be done by monitoring patients with confirmed Corona Virus Disease 2019 (COVID-19). The purpose of this study was to determine the neutrophil lymphocyte’s ratio in COVID-19 patients receiving convalescent plasma therapy. This research method is a quantitative observation using a descriptive approach. Quantitative observational research is used to analyze data in the form of numbers from the results of laboratory tests. The results obtained from 17 research subjects, namely the NLR values before convalescent plasma therapy were obtained in as many as 6 patients (35%) with normal NLR values below 3.13 and NLR values are increasing above 3.13 in as many as 11 patients (65%). Meanwhile, after convalescent plasma therapy, there were 8 patients (47%) with normal NLR below 3.13 and NLR values increasing above 3.13 as many as 9 patients (53%). This research concerns about changes in NLR values before and after convalescent plasma therapy, which before convalescent plasma therapy there were 6 patients with normal NLR values and after convalescent plasma therapy increased to 8 patients with normal NLR values which indicate a better good prognosis.
Effect of Bay Leaf (Syzygium polyanthum) Extract on Antioxidant Activity, MDA Levels, and Liver Histopathology Feature of Ethambutol Induced Wistar Rats Bambang Edi Suwito; Lea Maera Shanty; Retna Gumilang; Handayani Handayani; Renata Alya Ulhaq
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2471

Abstract

Bay leaf extract (Syzygiun polianthum) is one herbal elemnt that may be used to lessen liver function issues, lessen symptops of nausea, vomiting, discomfort and improve adherence and the effectiveness of tuberculossi treatment. The objective of this study was to determine the effect of bay leaf (Syzygium polyanthum) extract on antioxidant activity, Malondialdehyde (MDA) levels, and liver histopathology of ethambutol-induced Wistar rats. This research is a laboratory experiment. Antioxidant activity of Bay Leaf Extract was evaluated by comparing vitamin C with spectrophotometry methods. White Wistar rats were separated into 6 groups and uset to test the lebel of MDA and livers histopathology. Group 1 serves as the control group and received DMSO (placebo); Group 2 was received ethambutol 50mg/kg BW; Group 3 was received ethambutol and silymarine with dose 50mh/kg BW. Group 4-6 had been given ethambutol and extra ethanol extract of bay leaves of 75,150, dan 300mg/kg BW, respectively. The results of the spectrophotometry showed that the Bay Leaf Extract had antioxidant activity comparable to that of vitamin C, with an IC50 of 11.4 g ± (4.4%). One-Way ANOVA test results obtained p = 0.002 (p<0.05) while the Kruskal-Wallis test results obtained the Asymp. Sig value 0.105> 0.05. There was a significance difference in each group’s MDA levels (p=0.002). Although there was no significant difference in the liver histopathology of treated rats (p>0.05). While bay leaf extract significantly lowers MDA levels in ethambutol-induced Wistar rats, it has no discernible impact on the liver histopathology of ethambutol-induced Wistar rats. Bay leaf extract possesses antioxidant activity comparable to vitamin C.
The Effect of Cigarette Smoking Duration on Hemoglobin Level Measured with Cyanmethemoglobin Method Fitriani Kahar; Ririh Jatmi Wikandari; Irnawati Irnawati; Martha Silpa Penmaley
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2674

Abstract

In Indonesia, cigarette consumption is quite prevalent and has been become more common over time. According to Riskesdas (Baseline Health Research from Agency of Health Research and Development) study in 2018, the national percentage of people aged who smoke increased from 2016-2018, namely from 3.39% in 2016, 3.9% in 2017 and 9.65% in 2018. Smoking habits will negatively affect human health. Cigarette smoke contains various of different chemicals that are harmful to health, including nicotine addiction, tar (carcinogen or cancer-causing), and carbon monoxide. When inhaled, they form carboxyhemoglobin with Hb (Hemoglobin). When this occurs, the amount of Hb available for oxygen transport is reduced. By way of compensation, the body will produce more red blood cells. The objective of the study was to determine the hemoglobin levels in smokers with smoking durations of 3 years, 4 years and 5 years. This study is quasi-experimental. Determine hemoglobin readings using a photometer 5010 with the Cyanmethemoglobin method. Up to 20 samples were analyzed with SPSS version 20 with Anova analysis at 95% confidence level. The results of statistical analysis showed p-value <0.05, which means that there is a significant effect between variables and vice versa. Hb level comparison between durations of smoking are of 3 years with an average value of 14.8, Hb levels of 4 years with an average value of 14.53, and Hb levels of 5 years with an average value 15.94. The results showed that there was an effect of smoking duration on the results of the examination of hemoglobin levels in smokers of 3,4 and 5 years with the greatest effect being smokers with a duration of 5 years. The duration of smoking impact on hemoglobin levels.
Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples Ari Nuswantoro; Jessica Ningtyas Berlianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2683

Abstract

Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant.  Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.
Histopathological Profile of Therapeutic Doses of Methanolic Extract Combination of Tea and Mango Mistletoes in Cardiac of Preventive Hypertensive Rats (DOCA-salt) Model Durrotul Hasanah; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2693

Abstract

Tea and mango mistletoes have to lower blood pressure by improving oxidative stress and endothelial dysfunction. The flavonoid compounds found in these two mistletoes act as antihypertensives. Hypertension is a risk factors that influences the incidence of cardiac disease. This study examines the histopathological of the cardiac of hypertensive rats (preventive model) after treatment of methanolic extract combination of tea and mango mistletoes. This study used a completely randomized experimental design with 25 Wistar rats in total for five treatments, namely negative control (-), positive control (+), treatment 1 (dose of 50 mg/kg BW), treatment 2 (doses of 100 mg/kg BW), and treatment 3 (doses of 200 mg/kg BW). In this study, Deoxycorticosterone acetate (DOCA) were used to induce hyperttension in experimental animals. Data were analysed with one-way ANOVA test using JAMOVI (version 1.1.9.0) and posteriori. There was a significant difference between all groups (p<0.05). Administration of a methanolic extract combination of tea and mango mistletoes as the preventive model can significantly reduce hypertrophy of cardiac muscle cells. This effect occured with a dose of 50 mg/kg BW, the optimal dose to reduce hypertrophy of cardiac muscle cells in Wistar rats male.
Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C Juwy Trianes; Bastian Bastian; Dewi Hartati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2919

Abstract

Pneumonia is an infection that causes the largest death in children worldwide (South Sumatra Province), which occupies the 6th position. One of the laboratory re-identification of Klebsiella pneumonia is a multi-antibiotic testing. The high sensitivity antibiotic that is still used is amikacin. The uniqueness of the diameter of the inhibition zone using the antibiotic amikacin is that it is active against most gram-negative bacilli. But one of the several factors that affect the diameter of the inhibition zone is the incubation temperature. The optimum temperature for pathogenic bacteria is 37oC by using an incubator; however, several factors in the use of instruments such as frequent instability and disruption of installation lead to a need of incubation at 25oC. The study aimed to determine the difference in the diameter of the growth inhibition zone of K. pneumoniae after incubation at 37oC and 25oC. This research is an experimental research conducted at the Microbiology Laboratory of IKesT Muhammadiyah Palembang. The sample is K. pneumoniae which will be subjected to gram staining, biochemical tests, followed by a sensitivity test on Mueller Hinton media which is given an amikacin antibiotic disk and incubated at 370C and 250C in order to calculate the diameter of the zone of inhibition for the growth of K. pneumoniae bacteria. The data was analyzed using the alternative Wilcoxon test which obtained a p value of 0.014. The results of this investigation showed that K. pneumoniae incubated at 37°C and 25°C had a significantly different diameter of the growth inhibition zone.
Validation of Rapid Antibody (IgM – IgG) Test Kit for SARS-CoV-2 Infection in Surabaya, Indonesia Museyaroh Museyaroh; Puspa Wardani; Aryati Aryati; Evy Diah Woelansari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.3027

Abstract

At the beginning of the Corona Virus Disease 2019 (COVID-19) pandemic, rapid test examinations were widely used as a screening for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection. The purpose of this examination was to detect SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of these tests uses the immunochromatographic method. This study aims to determine the validity of immunochromatography. The study was conducted from August to September 2020. The sample used in this study was 100 patients. The research was conducted at Husada Utama Hospital Surabaya, Indonesia. According to the study's findings, the Zybio brand reagent kit has an accuracy of 85%, a sensitivity of 82%, a specificity of 88%, a positive predictive value of 87%, and a negative predictive value of 83%. In the group of patients who experienced clinical symptoms, < 7 had a sensitivity of 50%, specificity of 88%, positive predictive value of 60%, negative predictive value of 83%, and accuracy of 77.94% while the group of patients experiencing clinical symptoms > 7 days, had a sensitivity value of 100 %, specificity of 88%, positive predictive value of 84%, negative predictive value of 100%, and accuracy of 92.68%. Based on these results, the conclusion is that the Zybio brand reagent kit has a relatively high sensitivity, specificity, positive predictive value, negative value, and sample accuracy. In the group with clinical sensitivity < 7 days, the positive predictive value and accuracy are lower than the sample group with clinical symptoms > 7 days but have the same specificity.
Relationship of Albumin Concentrations with Sodium, Potassium, in CAPD (Continuous Ambulatory Peritoneal Dialysis) Patients at PHC Hospital Surabaya Dian Anggraini; Edy Haryanto; Syamsul Arifin; Ayu Puspitasari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.3252

Abstract

Kidney failure is caused by a permanent decline in kidney function and requires renal replacement therapy, one of which is Continuous Ambulatory Peritoneal Dialysis (CAPD). This study aimed to determine the relationship between albumin concentrations and sodium and potassium concentrations in CAPD patients. The study was carried out between January to May 2022. Cross-sectional research methodology is used in this study. It included 60 chronic kidney failure patients who had CAPD as therapy at PHC Surabaya Hospital. Then, patients were divided into three groups with an age interval of 40-50 years, 51-60 years, and 61-70 years in. The results of grouping CAPD patients according to the characteristics based on the age of CAPD patients aged 61-70 years had low albumin concentrations and low sodium concentrations A total of 12 people (66.67%), patients with large CAPD aged 51-60 years, had low albumin and potassium concentrations, namely 11 people (61.11%). CAPD patients aged 51-60 years mostly had low albumin and sodium concentrations in 13 people (72.22%). the conclusion is that in patients with renal failure on CAPD therapy, there is a decrease in albumin and sodium-potassium concentrations due to peritonitis (inflammation) which is associated with a measurable excess of fluid (overhydration) which causes more dilute sodium, causing hyponatremia and hypokalemia.

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