INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
Articles
73 Documents
<![CDATA[COMBINATION TEST OF CHINESE LEAF EXTRACT (Leucaena leucocephala folium) AND ALOE VERA INHIBITING GROWTH Escherichia coli ]]>
<![CDATA[Putra Rahmadea Utami]]>;
<![CDATA[Chairani]]>;
<![CDATA[Hendra Yudha]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v2i2.1577
<![CDATA[Chinese petai (Leucaena leucocephala folium) and aloe vera (Aloe vera L.) have medicinal properties among the plants. The objective of this study was to determine the number of inhibitory zones produced by the ethanol extract of Chinese petai and aloe vera on the growth of Escherichia coli. The research method was In-vitro Experimental Laboratory research design with the Kirby Bauer method. The samples used was Chinese petai and aloe vera with pure strains of E. coli. One Way ANOVA was used to compare the differences in inhibition of Chinese petai and aloe vera on the growth of E. coli. The results of a combination of Chinese petai and aloe vera extract test showed that there were significant differences in the concentration of 25 g, 50 g, 75 g, and 100 g (p <0.05). The ethanol extract of Chinese petai and aloe vera can inhibit the growth of E. coli. From the results of this study found that there was an interaction on the combination of ethanol extract of Chinese petai and aloe vera inhibiting the growth of E. coli with the most effective concentration being 100 g/mL. This study can find out the benefits of petai cina and aloe vera also the public will know the benefits and efficacy of Chinese petai and aloe vera leaves in medicine.]]>
<![CDATA[COMPARISON OF LEAVES AND BARK MANGKOKAN (Nothopanax scutellarium) EXTRACTS AGAINST THE DEATH OF HOUSE FLIES (Musca domestica) ]]>
<![CDATA[Darmadi]]>;
<![CDATA[Harni Sepriyani]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v2i2.1618
<![CDATA[Flies (Musca domestica) are vectors of disease that can transmit to humans. Generally, these flies controlled by using chemical insecticides. Excessive use of insecticide causes resistance and environmental pollution. The alternative way in fly control is to use natural ingredients from the extract of mangkokan stem bark and leaves (Nothopanax scutellaria). This study aims to determine the ratio of fly mortality after administration of ethanol extract of bark and methanol extract of mangkokan leaves (N. scutellarium). The method used in this study is a post-test only control group design. The average mortality rate of house flies using ethanol extract of mangkokan stem bark (N. scutellarium) was 5 for 55 minutes while methanol extract of mangkokan leaves (N. scutellarium) was 5 for 135 minutes with a p-value of 0.374 (p>0.05). It concludes that there is no significant difference in fly mortality with the ethanol extract of stem bark and methanol extract of mangkokan leaves (N. scutellarium). Result of this study shows that the ethanol extract of stem bark and mangkokan leaf extract (N. scutellarium) are equally potential natural insecticides.]]>
<![CDATA[PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING ]]>
<![CDATA[Nastasya Nunki]]>;
<![CDATA[Diah Titik Mutiarawati]]>;
<![CDATA[Endah Prayekti]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v2i2.1655
<![CDATA[Crystal violet and Safranin are dyes in Gram staining, which are carcinogenic. Alternative safe materials are needed to minimize the use of carcinogenic properties. Purple sweet potato (Ipomoea batatas L.) peels were the candidate of the alternative dye source because of its high anthocyanin pigment. The purpose of this study was to determine purple sweet potato (Ipomoea batatas L.) peels extract as an alternative to Gentian violet in Gram staining of bacteria. Extracts obtained from purple sweet potato peels studied with varying concentrations of 50%, 60%, and 75% for 1, 3, and 5 min as a substitute for Gentian violet on Bacillus sp. The parameters observed from this study based on visual field clarity, glass slide cleanliness, contrast, bacterial shape, bacterial colour. Each extract concentration compared with a control group using Gentian violet. The results showed that optimum staining in 50% concentration for 5 min, 60% concentration for 5 min, 75% concentration for 3 min, and 5 min respectively. The present study exhibited the potency of Ipomoea batatas L. peels extract as an alternative staining agent.]]>
<![CDATA[ASSOCIATION BETWEEN TRIGLYCERIDE SERUM LEVELS AND GLOMERULAR FILTRATION RATE (eGFR) IN PATIENTS WITH CHRONIC RENAL FAILURE AT JEMURSARI ISLAMIC HOSPITAL SURABAYA, INDONESIA ]]>
<![CDATA[Uswatun Hasanah]]>;
<![CDATA[Suhariyadi]]>;
<![CDATA[Andreas Putro Ragil Santoso]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v2i2.1668
<![CDATA[Chronic Renal Failure (CRF) is a progressive and irreversible decrease in kidney function. One risk factor that affects the progression of CRF is dyslipidemia due to abnormalities of lipid metabolisms. Dyslipidemia is characterized by the increase level of total cholesterol, LDL cholesterol, triglycerides, and a decrease in HDL cholesterol level. eGFR is a parameter for assessing the excretion function, by calculating the amount of filtrate produced by the renal glomerulus. This study aims to determine the relationship between serum triglyceride levels with eGFR in patient suffering from CRF at Jemursari Islamic Hospital Surabaya. This type of research is descriptive experimental with cross–sectional design. The study population was the patients with CRF. The primary data in this study was the results of examination of serum triglyceride levels in patients with CRF. The secondary data (serum creatinine levels, body weight, age, and sex) was obtained from medical records of CRF patients in February 2019. As much as 12% of patients were in the 40–50 years old category, 36% of patients were 51–60 years old, 44% of patients were 61–70 years old, and 8% of patients were 71–80 years old. 72% of patients were male. The mean triglyceride level was 146.68 mg/dL and the average eGFR was 19.86 mL/min/1.73m2. The relationships between triglyceride levels and eGFR was carried out by the Spearman–rho test where r value was –0.442 and value was 0.027. It was concluded that there was a significant negative correlation between serum triglyceride levels and eGFR in patients with CRF.]]>
<![CDATA[The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus ]]>
<![CDATA[Julia Nanda Puspita]]>;
<![CDATA[Rikhsan Kurniatuhadi]]>;
<![CDATA[Rahmawati Rahmawati]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v3i1.1700
<![CDATA[Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).]]>
<![CDATA[Ability of Ethanol Extract from Ajwa and Sukkari Dates (Phoenix dactylifera L.) in Inhibiting the Growth of Methicillin-Resistant Staphylococcus aureus (MRSA) ]]>
<![CDATA[Putra Rahmadea Utami]]>;
<![CDATA[Sri Indrayati]]>;
<![CDATA[Nur Hayatang]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v3i1.1848
<![CDATA[Staphylococcus aureus is a pathogenic bacterium that spread throughout the world and still a problem that continues to increase both in hospitals and the community. Infections due to S. aureus usually treated with antibiotics, but in some cases, several strains of S. aureus found to be resistant to antibiotics, such as Methicillin-Resistant Staphylococcus aureus (MRSA). Based on the previous research, the ethanol extract from Ajwa and Sukkari dates formed an inhibitory zone against the MRSA bacteria growth. This study aims to determine the inhibition of the ethanol extract from Ajwa and Sukkari variety of dates (Phoenix dactylifera L.) on the S. aureus growth. The ethanol extract from Ajwa and Sukkari dates with a concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL, and 20 mg/mL resulted in the same inhibition zone with a diameter of ≤ 6 mm which categorized as weak (resistant), whereas the positive control ciprofloxacin had a resistance zone with a diameter of 9 mm. This study results concluded that the ethanol extract of Ajwa and Sukkari dates only has a maximum concentration of 20 mg/mL, which is still classified as a low concentration and has not been able to inhibit MRSA bacteria growth.]]>
<![CDATA[Relation of Parasites in Soil with The Existence of Parasites on Farmer's Nails ]]>
<![CDATA[Edza Aria Wikurendra]]>;
<![CDATA[Merry Crismiati]]>;
<![CDATA[Globila Nurika]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v3i1.1850
<![CDATA[Worms is an infectious disease caused by parasitic worms that can endanger health. Worms that often infect and have a very detrimental impact are soil-borne worm infections or Soil-Transmitted Helminths. Soil-Transmitted Helminths still considered insignificant because it is considered not to cause harm or cause death. This study aims to determine the relationship of parasites in the soil with the presence of parasites on the nails of farmers Sumber Urip 1 Village Wonorejo, East Java, Indonesia. The research method was used observational analytic with a cross-sectional study design which involved 18 Sumber Urip 1 farmers in Wonorejo Village. The sampling technique used was Total Sampling. The bivariate analysis uses Pearson correlation with decision making using significant <0.01. The identification of parasites using the floating method in 18 soil samples contained 12 flattering pieces of hookworm larvae and roundworm eggs. While the results of parasite identification with sedimentation method in 18 nail samples of farmers, there are 11 positive hookworm larvae samples, i.e. Ancylostoma duodenale. The correlation test result showed a relationship between parasites in the soil and nails of Sumber Urip 1 farmers in Wonorejo village (significant as P < 0.01). The use of gloves and footwear (shoes) when working on agricultural land, wash hands with soap and brush nails so that dirt is lost, and consume worm medicine can prevent worms infection.]]>
<![CDATA[In Silico Analysis of Antiviral Activity and Pharmacokinetic Prediction of Brazilein Sappan Wood (Caesalpinia sappan L.) Against SARS-CoV-2 Spike Glycoproteins ]]>
<![CDATA[Dwi Krihariyani]]>;
<![CDATA[Edy Haryanto]]>;
<![CDATA[Retno Sasongkowati]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v3i1.1854
<![CDATA[Brazilein is one of the secondary sappan wood metabolites which can be used empirically as an antivirus. The SARS-CoV-2 spike (S) glycoproteins play significant roles in attaching and entering the virus into the host cell. This study aims to predict the antiviral activity and pharmacokinetic properties of brazilein of the sappan wood against the in-silico SARS-CoV-2 S glycoproteins with vitamin C as the reference compound. Molegro Virtual Docker 5.5 was used to predict antiviral activity by docking process. SARS-CoV-2 S glycoprotein with NAG ligand available in Protein Data Bank (PDB) (PDB ID: 7C01) was the receptor used. The pkCSM online tool was used to predict the pharmacokinetic properties and toxicity of brazilein. Data were analyzed on the target receptors by comparing the docking bond energies between NAG, brazilein, and vitamin C. The smaller the ligands’ bond energy to the target receptor, the more stable the bonds are. The bond energy of NAG, brazilein, and vitamin C was -59.2864 kcal/mol, -65.8911 kcal/mol, and -53.9093 kcal/mol, respectively. These results suggested that brazilein has a greater capacity as an antivirus compared to NAG and vitamin C. In silico test using the pkCSM online tool demonstrated that brazilein had strong pharmacokinetic properties and relatively low toxicity.]]>
<![CDATA[Chemometric Analysis of Serum Magnesium Calculations Using Mg-Xylidyl Blue-I Method Based on Molar Absorptivity ]]>
<![CDATA[Ally Kafesa]]>;
<![CDATA[Nadira Nur Hajah Lutfi]]>;
<![CDATA[Cep Wahyu]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v3i1.1876
<![CDATA[The concentration of magnesium is determined based on the absorbance of the Mg-Xylydil Blue-I complex solution use spectrophotometer. Based on the Lambert-Beer rule, the calculation of sample concentration is based on the formula A = Ԑ. b. C. Generally, the thickness of the cuvette (b) and the molar absorptivity (Ԑ) factor will be ignored because it is considered to have a fixed value, therefore the sample concentration is measured based on the ratio of the absorbance of the sample against the standard solution. However, the standard solution contains pure magnesium and has a different matrix than the sample matrix, so this condition can give analytical errors and lead to misinterpretation of the results. The purpose of this study was to determine the accuracy and the precision of serum magnesium calculation by the principle of the Mg- Xylydil Blue-I complex reaction based on molar absorptivity compared to the general method. This research uses comparative study design methods. The serum sample used was the patient's serum specimen who has a normal magnesium level. The results showed that the significance value of the paired t-test statistical was 0.000 (p < 0.05). The accuracy value (d%) of the calculation formula uses Ɛ is 0.00 and the precision value (CV%) is 0.53. While the accuracy value (d%) of the calculation formula without Ɛ is 0.00 and the precision value (CV%) is 0.38. Calculations based on molar absorptivity (Ɛ) can measure more significant serum magnesium than those calculated based on standard magnesium solutions.]]>
<![CDATA[Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli ]]>
<![CDATA[Endah Prayekti]]>;
<![CDATA[Suliati Suliati]]>;
<![CDATA[Dwi Agustin Wulandari]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v3i1.1906
<![CDATA[Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.]]>
