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Jurnal Pembelajaran dan Biologi Nukleus
Published by Universitas Labuhanbatu
ISSN : 24429481     EISSN : 26857332     DOI : https://doi.org/10.36987/jpbn
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media
Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali dalam setahun pada bulan Maret dan September. Jurnal ini menyajikan hasil penelitian dibidang Pendidikan Biologi dan sains Biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 22 Documents
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Search results for , issue "Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023" : 22 Documents clear
Development of Student Worksheets Based on Science Process Skills on Human Excretion System Materials Erma Lisa; Ira Suryani; Efrida Pima Sari Tambunan
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4285

Abstract

Development of Student Worksheets (LKPD) based on science process skills on human excretory system material presented in the form of an application on a smartphone which contains human excretion system material, stages of practicum activities and questions adapted to indicators of science process skills, which are expected to be able to train participants' thinking abilities educate. The research aims to find out: (1) the feasibility of developing science process skills-based LKPD (Student Worksheets): (2) the practicality of developing process skills-based LKPD (Student Worksheets); and (3) The effectiveness of developing LKPD (Student Worksheets) based on science process skills on human excretory system material. The development model in this study is 4D which consists of 4 stages, namely define, design, development, and disseminate the research subject of 30 students of class XI IPA MAS 12 Perbaungan. The research instruments are validation sheets for material experts, linguists, and media experts on feasibility testing, practitioner validation sheets to determine practicality, pretest and posttest sheets for effectiveness testing. The results of this study prove that the skill-based LKPD science process developed is declared very feasible with a percentage of 89.39%, very practical with a percentage of 81.94% and very effective with a high category of learning improvement that has been tested with n-gain with a score of 0.73 and excellent student response with a percentage of 81.58%. The results of this study prove that the developed science process skills-based learner worksheets are very feasible, very practical, and very effective to use in the learning process
Bioprospecting of Lactobacillus sp. Starter Culture from Colostrum Breast Milk for Probiotic Milk Production from Soybeans Mufti Hatur Rahmah; Masriany Masriany; Ramlah Ramlah; Isdaryanti Isdaryanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4427

Abstract

Lactobacillus sp. is a bacterium that naturally exists in various environments, including in breast milk colostrum. This bacterium belongs to a group of lactic acid bacteria that produce lactic acid as the main product of sugar fermentation so that it has probiotic properties that also play a role in the fermentation process. This study aims to produce probiotic milk using Lactobacillus sp isolates from breast milk colostrum  with soybean (Glycine max (L.) Merril.) as the base material and then test the total lactic acid content, pH value, reducing sugar and the number of lactic acid bacteria. The production of soybean fermented milk is based on the analysis of total lactic acid content by acid-base titration method with 0.1 N NaOH standard solution, pH value by using universal pH meter, reducing sugar test by spectrophotometric method of Nelson-Somogyi and the number of lactic acid bacteria by using Glucose Yeast Pepton Agar (GYPA) medium added with CaCO3 then calculated by using Standard Plate Count (SPC) method with TPC value (Total Plate Number) is 2.3. 1012 colonies/ml, 2.5.1012 colonies/ml, 4.5.1014 colonies/ml, 4.1.1014 colonies/ml. From the results of the study it is known that Lactobacillus sp isolates from breast milk colostrum fluid can ferment soybean milk (Glycine max (L.) Merril.)
Morphometric Study of African Spurred Tortoise (Centrochelys sulcata) Based on Age Group Tetty Barunawati Siagian; Muhammad Firman Bayu Prayogo; Danuja Widigdaya; Pungki Pungki
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4344

Abstract

Centrochelys sulcata are kept and bred to prevent extinction. The sulkata tortoises were identified to determine their respective identities by measuring them morphometrically. Morphometric studies on sulkata tortoises are still minimal, so a morphometric study of the sulkata tortoises is needed. The purpose of this study was to identify and describe the morphometry of sulcata tortoises with three different age ranges kept in ex-situ conservation. The research sample used 15 sulcata turtles which were divided into 3 groups namely group A (1 year), Group B (2 years), and Group C (3 years). The morphometric parameters measured were body weight, head, carapace, plastron, forelimb, hindlimb and bridge. The results showed that the average body weight of group A, B and C turtles was 2,179 kg, 1,289 kg and 0,167 kg respectively. The average maximum carapace length in a row for groups A, B, C, namely 11.64, 23.18 and 16.72 cm. The average plastron lengths for groups A, B, C were 9.08, 6.44 and 9.24 cm respectively. The average forelimb length in groups A, B and C were 3.04, 6.28 and 7.78 cm respectively. The average hindlimb length for groups A, B, and C were 1.86, 3.5 and 4.04 cm, respectively. The average bridge width for groups A, B and C were 4.36, 8.94 and 10.1 cm respectively. Based on the results of morphometric measurements of the three groups of sulcata tortoises, it was shown that group C had a larger morphometric size than groups B and A, this was due to differences in age and growth factors. Sulcata tortoises group C is 3 years old and belongs to the juvenile group. Sulcata tortoise groups A and B aged 1 and 2 years are included in the yearly group
In Vitro Antidiabetic Activity and Bioactive Ingredients of Apis mellifera and Apis dorsata Binghami Nest Extracts Henike Sibala; Orbanus Naharia; Nonny Manampiring; Yermia Semuel Mokosuli
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4511

Abstract

Indonesia ranks 7th out of ten countries with diabetes mellitus, which continues to increase yearly. Honeycomb is rich in secondary metabolites formed from plant resins (propolis) which contain alkaloids, flavonoids, saponins, tannins, steroids and triterpenes. The research aimed to analyze the comparison of flavonoid content and antidiabetic activity with the enzyme α-glucosidase In Vitro nest extract of A. mellifera and A. dorsata Binghami. The samples used were nests of A. mellifera from northern Toraja, southern Sulawesi and A. dorsata Binghami from Southeast Minahasa, North Sulawesi. Using 96% ethanol, HPLC method of flavonoid testing and in vitro antidiabetic activity testing using α-glucosidase enzymes. The flavonoid content results were based on the number of peaks produced and the retention time of A. mellifera produced 26 compounds, and A. dorsata Bingham produced 21 compounds. The results of in vitro antidiabetic tests with α-glucoside enzyme inhibitors obtained IC50 values from the nest extract of A. mellifera of 3.605 mg/L and the nest extract of A. dorsata Binghami of 4.992 mg/L. The extract from A. mellifera had better antidiabetic potential than that of A. dorsata Binghami, although the difference is insignificant.
The Development of Scratch Software-Based Interactive Learning Media on Regulatory System Material Nurhalizah Nurhalizah; Ummi Nur Afinni Dwi Jayanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4462

Abstract

The research aims to develop interactive learning media based on software scratch on the material of the regulatory system and to know the validity, practicality, and efficacy of the media. The research and development method with the ADDIE model is used in this research. The research instruments used are needs analysis in the form of teacher interview guides and student questionnaires, validation sheets consisting of media and materials expert validation sheets, and implementation tools in the form of effectiveness sheets, namely pre-test and post-test, and practicality sheets, namely teacher and student response questionnaires. Based on the results and discussion, the media expert`s validity test result is 87.33%, and the material expert's result is 87.50%, indicating that it is very valid. Then, the practicality test results of the teacher's responses received a percentage of 97.50%, which is very practical. The practicality test of student responses on a small scale yielded a rate of 93.25%, which is very practical, while the field test yielded a rate of 84.30%, which is quite practical. In the effectiveness test, the percentage of n-gain obtained was 84%, which means it was effective. The results show that scratch-based interactive learning media have proven to be valid, practical, and effective. This learning media can be implied as one of the supporting media for teachers in biology subjects, particularly on regulatory system material that may boost students' motivation for learning and academic success.
Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts Reinny Silvana Tuegeh; Herry Maurits Sumampouw; Meity Neltje Tanor; Yermia Semuel Mokosuli
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4514

Abstract

Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants
Analysis of Liver Histology Several Fish in the Air Kotok River in the Gold Mining Area, Lebong Regency Dian Fita Lestari; Eliza Febrianti; Shahnaz Sabrina Wulansari; Apriza Hongko Putra
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4020

Abstract

Fish have habitats that are easily exposed to contamination from the environment such as heavy metal waste which can cause changes in the structure and function of body tissues. One type of waste that enters the river in the Lebong Regency River is gold mining processing waste that uses mercury and cyanide. Heavy metals can enter organisms and accumulation and biomagnification of mercury can occur in the bodies of aquatic biota such as fish. There has been no research related to fish histology in the Lebong gold mining area, so the aim of this research was to determine the histological condition of the liver organs of several fish in the form of Hemibagrus nemurus, Rasbora sp, Trichogaster trichopterus, and Macrognathus circumcinctus in the Air Kotok river in the gold mining area of Lebong Regency. The research was conducted using the paraffin method with hematoxylin-eosin staining. Data were analyzed descriptively qualitatively based on the histology of the fish liver. The results of the research on the liver histology, it shows damage to the fish liver structure which includes vacuolization, congestion, pyknosis, dilated sinusoids, melanomacrophages and leukocyte infiltration. The damage to the hepatocytes of this fish can be used as a bio-indicator of exposure to toxic substances in the Air Kotok river, one of which is heavy metal activity from gold mining, which can be a threat to the health and survival of the fish.Keywords: Bengkulu, gold mining waste, heavy metal, hepa
The Effect of IAA and BAP on Root Induction of Cattleya Orchids Fauziyah Harahap; Kenari Br Sinuraya; Syarifuddin Syarifuddin; Cicik Suriani; Ayu Putri Ningsih; Syahmi Edi; Nusyirwan Nusyirwan
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4481

Abstract

Orchid is an ornamental plant commodity that has an important meaning in international trade. Until now, orchids are still the center of attention of farmers and lovers of ornamental plants, because of their bright potential as cut flowers and potted plants. The popular and widespread type of orchid is Cattleya sp. The Cattleya orchid is also called the queen of orchids because of its colorful flowers. Cattleya sp. orchids take a long time to propagate by seed, about 4 to 7 years, so other methods are needed to deal with them. Tissue culture is a method for isolating plant parts such as cells, tissues or organs, and cultivating them in an aseptic environment. The purpose of this study was to determine the optimal interaction of growth regulators indole acetic acid (IAA), benzyl amino purine (BAP), and their interactions with the root induction of Cattleya sp. Cytokinins interact with auxin in determining the direction of cell differentiation. In this research method, differences in concentrations of indole acetic acid (IAA: 0, 2, 4, 6 ppm) and concentrations of benzyl amino purine (BAP: 0, 0, 5, 1 ppm) were carried out. Parameters observed were the number of roots and root length. Data were analyzed descriptively and inferentially using ANOVA. The highest number of roots was obtained in the treatment medium containing 0.5 ppm BAP and 6 ppm IAA, which was 6.33. The highest root length was found in the B0I4 treatment (BAP 0 + IAA 4 ppm). The results of the analysis of the interaction variance between the IAA treatment and the BAP treatment were 0.005, meaning that there was a significant effect of the combination of IAA and BAP on the leaf length of Cattleya sp.
Formulation of Red Beet (Beta vulgaris. L) and Aloe Vera (Aloe vera) Gel Extracts as Anti-Aging Mutiara Dwi Lestari; Siti Maimunah; Andre Prayoga
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4478

Abstract

Anti-Aging can prevent or slow down the effects of skin aging. This study aims to formulate extracts of beetroot (Beta vulgaris. L) and Aloe Vera (Aloe Vera) in gel preparations and test their anti-aging effectiveness. This research was conducted at the Laboratory of Pharmaceutical Chemistry, University of Sari Mutiara Indonesia. The tools used are skin analyzer and moisture checker. B. vulgaris Samples from Brastagi, Karo Regency and A.vera taken from Rantauprapat, Labuhan Batu Regency. Methods of implementation Beet extraction was extracted with 96% ethanol solvent, and A.vera extract from the juicer. The irritation test on 12 volunteers used nettle extract cream with the highest concentration of 0.5%. Anti-aging activity testing used 12 female volunteers aged 19-22 years, who were divided into 4 groups, namely 3 volunteers (A; Blank), administration of beetroot extract gel (B. vulgaris. L) and A.vera (B) : 3%), (C: 6%), and (D: 9%). 3. Parameters measured include moisture content, number of pores and wrinkles. Data distribution was analyzed using the One sample Kolmonogrov-Smirnov Test method. Then proceed with the analysis of the One-Way Anova method. The results of research on beet (B. vulgaris. L) and Aloe Vera (A.vera) gel preparations can be formulated in a gel dosage form. pH 6.9, W/O emulsion type, viscosity 2500-3500 cps, non-irritating, and stable on storage for 28 days. Beet fruit extract (B. vulgaris) and aloe vera viscous extract (A.vera) can be formulated in the form of an anti-aging gel that can provide an anti-aging effect at the best concentration, namely at a concentration of 9% by increasing skin moisture to 40.24%; smaller pores 0.53%; and reduce wrinkles by 0.33%
Anthocyanin, Antioxidant and Metabolite Content of Butterfly Pea Flower (Clitoria ternatea L.) Based on Flowering Phase Juswardi Juswardi; Rina Yuliana; Nina Tanzerina; Harmida Harmida; Nita Aminasih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4064

Abstract

The butterfly flowers (Clitoria ternatea L.) are used as natural dyes and herbal medicines because they contain metabolites, anthocyanins and antioxidants. The content of these metabolites is influenced by the phase of flower development. This study aims to determine the levels of anthocyanins, antioxidants and metabolites of butterfly pea flowers from different flowering phases. The Butterfly flower collection was taken from the village of Mulyaguna, Teluk Gelam, Ogan Komering Ilir, South Sumatra. The extraction was carried out with 70% ethanol solvent, and determination of the amount of anthocyanin content by spectrophotometry, antioxidant content by DPPH method, and metabolite compounds by GC-MS. Data on anthocyanin and antioxidant content were analyzed with averages and standard deviations, and GC-MS chromatograms were traced for compounds with reference to the PubChem, KEGG, ChEBI, PlantCyc, and Spectrabase websites, which then determined the dominant compound group. The results of the study on blooming butterfly pea flowers found that the antioxidant content was 6.58 ppm, higher than that of bud flowers, which were 2.55 ppm, and wither flowers, which were 1.74 ppm. The anthocyanin content of the blooming butterfly pea flower was 40.33 ppm, the withering flower was 4.36 ppm, and the bud flower was 3.60 ppm. The dominant metabolites were identified as fatty acids, organic acids, aromatics and flavanoids, followed by differences in antioxidant and anthocyanin content in the flowering phase of the butterfly pea flower

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