Berkala Penelitian Hayati
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
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<![CDATA[Amplifikasi Gen CRY1 dan Analisis Genom Isolat Bacillus thuringiensis Lokal ]]>
<![CDATA[Dwi Suryanto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/229
<![CDATA[A study on amplification of Cry1 gene and genome analysis of local isolate of Bacillus thuringiensis TU1 has been done. Amplification was done for cryI gene by PCR-technique. Genome analysis was performed using pulsed-field gel electrophoresis. Insecticidal specificity assay of the isolate to larvae was done in larvae of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. Isolate of commercial strain, B. thuringiensis var kurstaki strain HD-7, was used as control for amplification of cryI gene, genome analysis, and insecticidal specificity assay. The result showed that insecticidal specificity assay of the isolates of both TU1 and commercial have similar spectrum of toxicity to the larvae. Amplification of cry1 gene resulted to a fragment of approximately 550 bp in both TU1 and commercial. Genome profiles of TU1 and commercial strain were similar.]]>
<![CDATA[STATUS KUALITAS SUNGAI MUSI BAGIAN HILIR DITINJAU DARI KOMUNITAS FITOPLANKTON ]]>
<![CDATA[Hilda Zulkifli]]>;
<![CDATA[Husnah]]>;
<![CDATA[Moh. Rasyid Ridho]]>;
<![CDATA[Suhodo Juanda]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/231
<![CDATA[Musi river is the main source of water for society and industry on the downstream area, and also becomes the waste disposal area for domestic/industry. The objective of this study was to evaluate the status of Musi river on the downstream area based on phytoplankton community (from the Pulokerto district to downstream of musi river on Tanjung Buyut Village). The sample of water surface and plankton were taken compositely on 18 stations. The water quality analysis is held on LIPI Bogor, while the identification of microscopic community of phytoplankton is held on BRPPU Mariana. The result of research showed that phytoplankton community on observed locations comprises of Chlorophyta with 18 genus (45.34%), followed by Cyanophyta with 7 genus (26.09%), Baccillariophyta with 14 genus (25.81%) and Euglenophyta with 2 genus (2.17%). The Oscillatoria (Cyanophyta) is found on most of observed locations. Based on the examination of saprobic status, Pulokerto station is categorized as “moderate polluted” or (ά/ß –mesosaprobik dan ß/ά –mesosaprobik), while closer to downstream of Musi river is categorized as “low polluted” or (oligo/ß –mesosaprobic).]]>
<![CDATA[PERAN PROTEIN MEMBRAN LUAR 55 kDa Salmonella typhi ISOLAT JEMBER SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN ]]>
<![CDATA[Diana Chusna Mufida]]>;
<![CDATA[Candra Bumi]]>;
<![CDATA[Heni Fatmawati]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/234
<![CDATA[Salmonella typhi is an obligate pathogen that is usually found in clinical specimen from typhoid fever patients. The pathogenic mechanism of bacteria are not fully elucidated especially its potential activity of the outer membrane protein (OMP) as hemaglutinin and adhesion molecule. After identification, bacterial isolate of outer membrane protein fraction 12,5% SDS-PAGE were used to isolate OMP followed by hemaglutinin test and in vitro adhesion test. The study showed that the 55 kDa protein of S. typhi is a hemaglutinin protein that could agglutinate, Wistar mouse erythrocytes. The 55 kDa OMP is also an adhesion protein showed by its activity to adherence to Wistar mice’s enterocyt. The increased dose of 55 kDa OMP will decrease the amount of S. typhi bacteria adherenced to Wistar mice’s enterocyt. Outer membrane protein 55 kDa S. typhi as hemagglutinin and also adhesion protein.]]>
<![CDATA[GROWTH PERFORMANCE AND COCOON PRODUCTION OF SILKWORM (Bombyx mori L) ON DIFFERENT FREQUENCY OF FEEDING AND AGE OF LEAVES ]]>
<![CDATA[Priyantini Widiyaningrum]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/235
<![CDATA[The aim of this study was to evaluate the effects of different frequency feeding and aging of leaves on increasing weight of silkworm instar IV-V and cocoon production. The research was conducted in Cultivation Center of Silkworm Candiroto, Temanggung. The research design was Factorial Design with three level of age from the leaves (the third – fourth leaves from tip of leaf; fifth-sixth leaves from tip of leaf; sevent-eight leaves from tip of a leaf, and three level of feeding frequency 3, 4, 5 times/day). The parameter observed were average daily gain (ADG), weight of cocoon, and weight of empty cocoon. The data gained were analyzed by analysis of variance and to find out the difference caused on the treatments, Duncan Multiple Range Test was used. It is concluded that the different frequency of feeding and age of leaves is significant (P < 0.05) in ADG of silkworm and weight of empty cocoon. The seventh- eight leaf from tip of a leaf, and frequency of feeding five times/day resulting ADG and empty cocoon of the best.]]>
<![CDATA[KARAKTER PROTEIN ICP11 PADA DNA UDANG VANNAMEI (Penaeus vannamei) YANG TERINFEKSI White Spot Syndrome Virus (WSS V) ]]>
<![CDATA[Yuni Kilawati]]>;
<![CDATA[Win Darmanto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/236
<![CDATA[The aim of this study was to support policy of government to lessen import and improve export of non migas presented characteristic of genetic of white prawn mains (Penaeus vannamei) so that this product can be yielded in Indonesia with high quality which do not fail with import product. Special goals wishing to be reached at this research is to analyze the genetic characteristic DNA with Polymorphism Chain Reaction (PCR) and primer ICP11. The background of this research was difference among vannamei prawn DNA characteristic that have infected by viruses. With experimental methods ten healthy post larva of white prawn cultured in aquarium and invected by WSSV virus in 1ml/10 l marine water dossage with three time replication during 7 days in culture. To know these infection, the sample were analyze with specific primer ICP11.]]>
<![CDATA[IDENTIFIKASI DAN KLASIFIKASI BAKTERI AMILOLITIK ISOLAT TG12, TG19, DAN TG31 PENYEBAB KEMASAMAN PADA TEPUNG SAGU BASAH BERDASARKAN ANALISIS GEN 16SrDNA ]]>
<![CDATA[Tri Gunaedi]]>;
<![CDATA[Sebastian Margino]]>;
<![CDATA[Langkah Sembiring]]>;
<![CDATA[Rarastoeti Pratiwi]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/237
<![CDATA[16SrDNA gene were known essentialy for procaryotic life involved bacteria. The gene very concerved such as usefull for bacterial identification and classification in phylogeny tree constructed. The object of this research were identified and cllassified amylolitic bacteria TG12, TG19 and TG31 isolates, causers sourness on raw starch sago by 16SrDNA gene sequences analysis approach. The native isolates from raw starch sago under traditionality processing arround Jayapura and selected depend on activity amylolitic and organic acid productivity. Before DNA genom extraction, isolates were throught out generic assignment analysis. Futhermore DNA genom were amplified and purified by PCR with 27f and 1529r primers. The pure of DNA was sequenced by ABI PRISM 310 DNA sequencer with internal primers 27f, 357f, 790f and 1230f. The generic assignment resulted those isolates related with Bacillus. The 16S rDNA data were aligned with corresponding available Bacillus sequences retrieved from the NCBI database using the CLUSTAL X software. Phylogeny tree was constructed by PHYLIP programme and visualized by Treeview programme. Phylogenetic trees were and the extended the value of 16S rDNA sequencing in amylolitic bacteria causing sourness on raw starch sago. Completed 16S rDNA sequence data showed that two of the tested isolate TG12 formed a distinct center of diversity with Bacillus substilis DSM 10 AJ276351, isolate TG19 with Bacillus substilis strain 1778 EU982544 and TG31 similar genetic with Bacillus cereus strain WJL-063 FJ527559. Identification based on 16S rDNA gene sequences of amylolitic bacteria causing sourness on raw sago starch provided a powerful way of uncovering genetic of strain within the spesies Bacillus substilis and Bacillus cereus.]]>
<![CDATA[BIOFERTILISASI BAKTERI Rhizobium PADA TANAMAN KEDELAI (Glycine Max (L) Merr.) ]]>
<![CDATA[Tini Surtiningsih]]>;
<![CDATA[Farida]]>;
<![CDATA[Tri Nurhariyati]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/238
<![CDATA[The aim of this research want to know the influence of the addition Rhizobium bacteria species, dose and combination both of them, on growth and production of soybean plant (Glycine max (L) Merr.). The experimental design of this research was factorial design 4×2, 4 species of Rhizobium are R1 = Rhizobium japonicum, R2 = R. phaseoli, R3 = R. leguminosarum, R4 = mixture of R1, R2 and R3, and 2 dose of inoculan Rhizobium (D1 = 5 m/plant, and D2 = 10 ml/plant) with 1010 sel bacteria/ml and 5 replications. Independent variable is species of Rhizobium, dose of inoculan Rhizobium and combination both of them. Dependent variable is dry matter, weight of nodules and dry weight of seeds. The harvest data was analyzed by Kruskal-Wallis Test using 5% level (a = 0.05) followed by Mann- Whitney Test. The result of this research show that species of Rhizobium, dose of inoculan Rhizobium and combination both of them present insignificant result (a > 0.05) on soybean growth and production, but the mixture of Rhizobium species with high level dose of bacteria, present better result than single species with low dose of bacteria.]]>
<![CDATA[DAYA ANTIMIKROBA EKSTRAK Lecythophora sp., ENDOFIT YANG DIISOLASI DARI Alyxia reinwardtii ]]>
<![CDATA[Noor Erma Sugijanto]]>;
<![CDATA[H. Putra]]>;
<![CDATA[F. Pritayuni]]>;
<![CDATA[N. Albathaty]]>;
<![CDATA[Noor Cholies Zaini]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/239
<![CDATA[The antimicrobial potential of two endophytic fungi isolated from Alyxia reinwardtii BL, towards selected bacteria (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis FNCC 0059, Pseudomonas aeruginosa ATCC 27853, Salmonella typhi) and fungi (Candida albicans) was tested using ethyl acetate, n-hexane and n-buthanol extracts of fungi cultivated under malt extract liquid fermentation. The extracts were evaluated for its antimicrobial activity by disc diffusion method followed its MIC by agar dilution. Bioautography assay for activity-directed fractionation were also conducted against Bacillus subtilis and Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. Streptomycin sulphate and myconazole used as reference standards. Lecythophora sp. strain 30.1 and 30.5 have broad spectrum antimicrobial activity and a potential source of new classes of antibiotics that could be useful for medicines and biological control agents.]]>
<![CDATA[BIOLOGI REPRODUKSI IKAN JUARO (Pangasius polyuranodon) DI DAERAH ALIRAN SUNGAI MUSI, SUMATRA SELATAN ]]>
<![CDATA[Yunizar Ernawati]]>;
<![CDATA[Eko Prianto]]>;
<![CDATA[A. Ma'suf]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/240
<![CDATA[Study on biology reproduction was expected to give basic information for management, such as for aquaculture, capture fisheries and conservation of P. polyuranodon in Musi river. Sampling were conducted on June 2006, August 2006, January 2007, and July 2007 in Musi river by using gillnet. Data analysis included length-weight relationship, sex ratio, condition factor, gonad maturity stage, Gonadosomatic Index (GSI), fecundity, and spawning type. Total number of collected P. polyuranodon was 51, consisted of 23 male and 28 female. Total length of fish was ranged in 85–511 mm. Length-weight relationship of male and female was W = 0.00002L2.8062 and W = 0.0002L2.8215 respectively. The result from t-test analysis showed that both of sexes have allometric negative growth. Sex ratio thoroughly was balance (1:1). Mean of factor condition relatively increased by the increasing of gonad maturity stage. At that time P. polyuranodon was entering spawning time. June and August was predicted as the spawning time of this species. Gonadosomatic index of female was much higher than the male. Fecundity of female was ranged between 616 and 7,059. Based on distribution of egg diameter, this species was total spawner.]]>
<![CDATA[VARIASI TEMPORAL MAKANAN IKAN SEPAT LAYANG (Trichogaster leerii, Blkr. 1852) DI HUTAN RAWA GAMBUT DESA DADAHUP, KALIMANTAN TENGAH ]]>
<![CDATA[Ahmad Zahid]]>;
<![CDATA[MF. Rahardjo]]>;
<![CDATA[Sutrisno Sukimin]]>;
<![CDATA[Lenny S. Syafei]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/241
<![CDATA[Changes in habitat quality and resource availability associated with seasonal variation in hydrology strongly influences ecological interactions. The study was conducted from July to December 2007 which aiming to described prey item of Trichogaster leerii in peatswamp forest district of Dadahup, Central Kalimantan based on temporal variation (low and high water period). Each month, research zone, and size classes; the stomach contents on consisted predominantly of periphyton, such as Diatoma; Fragillaria; Nitzschia; Navicula; and Tabellaria. Diatoma was the dominance in the stomach contents and prey-selectively ingested of T. leerii. Size classes-based, the diet of T. leerii was least similar during falling and rising water. Feeding strategy of T. leerii was generalized. No different of diet breadth during the low- and the high-water period. Diet overlap between pairs of size classes of T. leerii was high in low- and high water period.]]>
