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Inventory Of Predatory Insects In Banana Germplasm Umbulharjo Yogyakarta Ichsan Putra; Hastin Nur Samsia Sahnan
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

One of the beneficial insects in agriculture and plantations is a predator. Using natural predators to control pests and diseases certainly has advantages. The purpose of this study was to calculate the level of predatory insect diversity in banana germplasm plantations and find the most abundant and least abundant predatory insects. The independent variable in this study is the banana germplasm plantation area, and the dependent variable in this study is the diversity and dominance of predatory insects found in the research location. Sampling in this study using a random method. The sweep net was in the morning, while the pitfall was collected, installed in the morning and taken in the afternoon. Predatory insect data analysis used in this study is to use the scan now-wiener diversity index and the Simpson dormancy index. The results showed that the predatory insects that mainly were found were the species, Paratrechina longicornis while the least that was found was the species Ischiodon scutellaris. From other results, namely the level of predatory insect diversity obtained, the results show that Yogyakarta banan germ plantation have moderate diversity of predatory insects. The conclusion of this study is that there is a disturbance in the Giwangan Banana Germplasm Garden Yogyakarta due to insects, so that the diversity index is moderate, while the predominance of predatory insects is 0.484, which means that in the ecosystem, one predatory insect is found that dominates.

The Effect of Arabica Coffee Fruit Skin Extract (Coffea arabica L.) on the Histopathology of Mice (Mus musculus L.) Liver Induced by Ethanol Dini Cahyani; Rita Maliza; Haris Setiawan
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

Excessive alcohol consumption can lead to serious live injury. Alcohol is a source of free radicals that can cause oxidative stress. The provision of antioxidants can counter the effects of free radicals that enter the body. The skin of the Arabica coffee fruit contains antioxidant compounds that can be used as a hepatoprotective in the liver. The skin of the Arabica coffe fruit contains antioxidant compounds that may have a hepatoprotective effect on the liver. This study aimed to determine the effect of extract of arabica coffee fruit skin on necrosis, infiltration and haemorrhage in the liver tissue of 15% EtOH-induced mice for 15 days. The animals used for this study were eight weeks old male Balb/c strain mouse weighing approximately 30-40 grams. This research is an experimental study. There were five groups: the control group (K), the 15% ethanol treatment group (E), and the ethanol-induced group and given different doses of arabica coffee skin extract: 125 mg/kg BW. (A), 250 mg / kg BW (B) and 500 mg / kg BW (C). Observation and identification of necrosis, hepatocyte infiltration and haemorrhage were carried out by using Hematoxylin-eosin staining. The data analysis used was One way ANOVA and continued by Duncan Multiple Range Test (DMRT). The histopathological observations showed that the extract of arabica coffee fruit skin at a 250 mg/kg BW dose reduced the number of cell necrosis. There was no infiltration of inflammatory and hemorrhagic cells in the liver of 15% EtOH-induced mice. This study concludes that the extract of the skin of the Arabica coffee fruit has hepatoprotective potential in 15% EtOH-induced mice.

Comparison of Total Phenolic, Flavonois Content, and Antioxidant Activity Artocarpus heterophyllus Lamk. Methanol Extract from Sleman and Bantul District Ambar Pratiwi; Riska Susilowati
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

Antioxidant compounds can inhibit free radicals that are harmful to body health. The side effects of natural antioxidant compounds are safer than synthetic antioxidants. The source of natural antioxidant compounds can be obtained from jackfruit because it contains phenolics, flavonoids, sterols, and tannins. The study was conducted to determine the highest total phenolic content, total flavonoids, and antioxidant activity in the methanol extract samples of jackfruit from Sleman and Bantul Regencies. The extraction technique used maceration with methanol (1: 5) solvent. The phenolic content was tested by the Follin-Ciocalteu method at a wavelength of 745 nm, while the flavonoid content was tested using the AlCl3 method at a wavelength of 435 nm. The antioxidant activity was determined by the diphenylpicrylhydrazyl (DPPH) method at a wavelength of 517 nm. The results showed that the total phenolic and flavonoid content of the methanol extract of jackfruit from Bantul Regency was 1.927 m GAE / mg and 0.261 m QE / mg higher than Sleman Regency 1.353 m GAE / mg and 0.0913 m QE / mg and the highest antioxidant activity name in the sample extract from Bantul Regency, the IC50 value was 581.5 mg / mL and based on the results of statistical tests there were significant differences in content. This study concludes that the total phenolic content and total flavonoids as well as the antioxidant activity of the methanol extract of jackfruit from the Bantul Regency were higher than from Sleman Regency.

Prevalence and Intensity of Anchor Worm (Lernaea cyprinacea L.) Ectoparasite in Koi Fish (Cyprinus carpio L.) at Bantul Koi Farm D.I. Yogyakarta Wida Afriandini; Nurul Suwartiningsih
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

One obstacle in the cultivation of Koi fish (Cyprinus carpio L.) in Bantul Koi Farm D.I. Yogyakarta is a disease caused by parasites of anchor worms (Lernaea cyprinacea L.). Therefore, this study observed the prevalence and intensity of anchor worm ectoparasites in Koi fish in Bantul Koi Farm. Taking fish samples using the purposive sampling technique. Each of three fish individuals was taken as replication from three populations, namely Showa, Shiro and Saragoi, with a size of 28-36 cm. The prevalence and intensity are determined by the observation method by counting the number of anchor worms found. Inferential statistics carried out data analysis of the number of ectoparasites in each population. The results showed that the highest anchor tick ectoparasite prevalence was found in the Shiro population (100%) and the lowest in the Saragoi population (33%). The highest intensity was in the Showa population (4 individu/ fish), and the lowest was in the Shiro population (2 individu/ fish), although all of them were in a low category. The highest number of ectoparasites was obtained from the Showa population (8 individuals) and the lowest in the Saragoi population (3 individuals), but it was not statistically significant. The high prevalence in the Shiro population is due to the high stocking density. Anchor flea ectoparasites attacked the skin and fins of Koi fish and were not found to attack gills. The conclusion of this study is the highest prevalence of anchor tick ectoparasites was found in the Shiro population (100%) and the lowest in the Saragoi population (33%). The highest intensity was found in the Showa population (4 individu/ fish) and the lowest in the Shiro population (2 individu/ fish), even though all of them were in a low category.

The Effect of Online Learning Policy on the Composition of Bird Species in the Rice Field around Campus 4 of Universitas Ahmad Dahlan Ragil Yoga Mandhala Wicaksana; Inggita Utami
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

The Covid-19 pandemic, which began in March 2020, resulted in enacting an online learning policy on campus. This study aimed to analyze the effect of online lecture policies on the composition of birds in the rice fields around Campus 4 of Universitas Ahmad Dahlan (UAD) Yogyakarta. Primary data collection was carried out at six points with the point count method in September 2020 and January 2021. Data analysis was carried out descriptivel by comparing observations with secondary data in September 2019 and January 2020, right before the Covid-19 pandemic. The results showed that the bird species present increased from 14 species to 32 species. The different paddy fields and rice morphology conditions in September and January also affect the bird species present. All birds present were not protected, and only one species, Acridotheres javanicus, was included in the IUCN Red List vulnerable category. Data collection on bird species must be carried out every year to monitor the presence of waterbirds in urban areas.

Methanolic Extracts of Waru Leaves (Hibiscus tiliaceus L.) for Controlling Armyworms (Spodoptera litura F.) Diah Asta Putri; Arib Cahyo Saputra
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

Botanical extracts have been reported as biopesticides, which act as antifeedants, insect growth regulators and repellents. As biopesticides, botanical extracts offer a more sustainable solution to pest control as chemical pesticides have a residual problem and are prone to pest resistance. The present study was conducted to evaluate the efficacy of waru leaves extract to control S. litura larvae in vitro conditions. The extract was prepared by maceration with methanol as a solvent. Third instar larvae of S. litura were randomly grouped into 6 treatments consisting of 0%, 3%, 6%, 9%, 12% and positive control (Lannate). Each treatment consisted of 3 replications. Leaf Dipping method was used in the treatment. Observations were made for up to 4 days. The parameters observed were changes in larval morphology, mortality, LC50 and LT50. Data were analyzed using One Way ANOVA (followed by LSD) and Probit analysis. The results showed that the highest activity of waru leaf extract was found at a concentration of 12%, which caused a mortality of 53% larvae. The LC50 value was at a concentration of 11.72%, and the LT50 value was 3.287. It can be concluded that the leaf extract has the potential to control S. litura larvae.

Species Diversity and Distribution Area of Lichen in Baturraden Botanical Garden, Banyumas Siska Dwi Yulianti; Eko Retno Mulyaningrum; Praptining Rahayu
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022)
Publisher : Universitas Ahmad Dahlan

Lichen is a symbiotic organism between fungi and algae which is used as a bioindicator of air pollution. This study aims to determine the diversity of species and distribution areas of lichen in Baturraden Botanical Garden, Banyumas Regency by using purposive random sampling method. Sampling was carried out at three stations, namely flora of Java forest (Javanese mountain plants), resin tree forest (damar forest), and natural forest. Based on the results of the research at the three observation stations, 10 species of lichen were found. The lichens found belonged to 8 families including Cryptothecia striata (Family Arthoniaceae), Dirinaria picta (Family Caliciaceae), Graphis scripta and Diorygma poitaei (Family Graphidaceae), Parmotrema austrosinense and Parmotrema perlatum (Family Parmeliaceae), Psilolechia lucida (Family Psilolechiaceae), Lepraria caeosioalba (Family Stereocaulaceae), Normandina pulchella (Family Verrucariaceae), Opegrapha atra (Family Opegraphaceae). The thallus types of lichen found were foliose, crustose, and squamulose species. The average value of the species diversity index from the three stations is H' = 1.493 in the moderate abundance category. The distribution of lichens in Baturraden Botanical Garden is influenced by climatic factors (light intensity, air temperature, humidity), topographic factors (altitude) and environmental conditions.

Research trends in isolation and identification of bacteria from Indonesia with various roles: Review Article Moh. Mirza Nuryady; Aisha Aisha; Diva Aulia; Aulia Savitri
Journal of Biotechnology and Natural Science Vol. 1 No. 2 (2021)
Publisher : Universitas Ahmad Dahlan

Bacteria are agents that can be used widely and are genetically easy to manipulate and reproduce. Many studies related to the isolation and identification of bacterial isolates from Indonesia have been carried out for various purposes. This research is still ongoing and has never been informed about the abundance of data from previous studies. The purpose of this study is to provide an overview of research topic trends related to the isolation and identification of bacterial isolates from Indonesia. The method used in this review is by setting inclusion and exclusion criteria and selecting a random sample of articles for analysis. The results of a review of research trends in isolation and identification of bacterial isolates from Indonesia showed four main topics discussed, namely the topics of food processing, agriculture, health, and bioremediation. Analysis of 41 articles shows that the most common discussion is the exploration of Lactate Origin-producing bacteria, the role of improving food quality. Furthermore, it was identified that the most isolated bacterial isolates came from food and plants, with 14 publications from a total of 41 articles. It can be concluded that exploratory research on Lactic Acid Bacteria for improving the quality of food products is currently the most studied topic by researchers in Indonesia.

Analysis of welfare levels, ecology, and animal management in Seblat Elephant Training Center, Bengkulu−Indonesia Cassytta Dhiya Imtiyaaz; Amir Syarifuddin; Joko Triwanto; Rosyid Ridlo Al Hakim
Journal of Biotechnology and Natural Science Vol. 1 No. 2 (2021)
Publisher : Universitas Ahmad Dahlan

The Sumatran elephant (Elephas maximus sumatranus) is a large mammal protected species and categorized as endangered according to the IUCN Red List. Habitat management includes control and regulation forest vegetation, open areas, water sources, salting sites, and resting areas for good animal welfare. The Seblat Elephant Training Center (SETC) is a conservation area to protect the Sumatran Elephant from extinction and is an ecotourism destination. This study aimed to analyze the welfare level and management of the Sumatran elephant in the SETC. This research used structured interview method to determine the indicators of the animal welfare level. Calculation of feed productivity and secondary data collection were used to improve the welfare level of the Sumatran Elephant. The research result stated that the welfare level of Sumatran elephant was classified as excellent (the implementation achievement value is 74.3%). In addition, the development of observations suggests that freedom from environmental discomfort needs to be improved by providing quarantine cages and reproductive cages to support natural behavior. Environmental conditions also support the welfare level of the Sumatran elephant. In conclusion, this facility is properly conducive to the welfare of Sumatran elephants.

The Process of Making Nata de Salacca from Honey Salak Fruit (Salacca edulis Reinw) with the Application of Biotechnology Techniques Anisa Novia Irwani; Yasmin Auranina Oskandar; Anisa Novia Irwani; Dian Putri Rahmawati; Tara Puri Ducha Rahmani
Journal of Biotechnology and Natural Science Vol. 1 No. 2 (2021)
Publisher : Universitas Ahmad Dahlan

Salak is typical Indonesian fruit commonly found in tropical countries. This fruit can fill global fruit market via exports to various countries. One of the varieties is "salak madu" ("honey salak"), whose scientific name is (Salacca edulis Reinw), which has higher economic value than "salak pondoh super". Some of the characteristics of honey salak are thick flesh, high water content, and sweet taste. Due to its high water content, honey salak rots quickly, resulting in useless waste and causing unfavorable smell. As such, utilization of overripe salak fruit (ripe close to rotten) can be done with implementation of biotechnological approach using Acetobacter xylinum to make nata. This study is aimed at utilizing overripe honey salak (ripe close to rotten) by processing it into Nata de salaca and identifying the factors affecting the production of Nata de salaca. The study used systematic review method by reviewing and examining previously published journal articles. The results of this study suggest that overripe salak can be utilized to make Nata de Salaca with the help of Acetobacter xylinum. Based on ratio of salak substrate to water, a good Nata has the ratio of 1 : 4. 1. Whereas, based on the factor of sprout extract additive material, it is 3%. Dan Whereas based on optimum duration of fermentation, it is 10 minutes.

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Ichsan Luqmana Indra Putra

Contact Email
ichsan.luqmana@bio.uad.ac.id

Phone
+6283869807245

Journal Mail Official
journal.bns@bio.uad.ac.id

Editorial Address
Jl. Ahmad Yani, Ring Road Selatan, Kragilan, Tamanan

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ichsan Luqmana Indra Putra

Contact Email ichsan.luqmana@bio.uad.ac.id

Phone +6283869807245

Journal Mail Official journal.bns@bio.uad.ac.id

Editorial Address Jl. Ahmad Yani, Ring Road Selatan, Kragilan, Tamanan

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ichsan Luqmana Indra Putra

Contact Email
ichsan.luqmana@bio.uad.ac.id

Phone
+6283869807245

Journal Mail Official
journal.bns@bio.uad.ac.id

Editorial Address
Jl. Ahmad Yani, Ring Road Selatan, Kragilan, Tamanan

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA