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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 12 Documents
 1   2 
Search results for , issue "Vol 15, No 4 (2021): December" : 12 Documents clear
COMPARISON OF DIFFERENT COMMERCIAL SEROLOGY KITS FOR THE DETERMINATION OF SEROPOSITIVE TOXOPLASMOSIS IN CATTLE IN INDONESIA Didik T Subekti; Sisca Valinata; Sulinawati Fong
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.359 KB) | DOI: 10.21157/j.ked.hewan.v15i4.21716

Abstract

This research aims to explain the evaluation of the differences in four commercial kits for the detection of serological toxoplasmosis used in Indonesia.  The results of the study found that the toxoplasmosis seropositivity determined by the four commercial kits showed a significant difference (P0.05).  Seropositive toxoplasmosis obtained using Pastorex, Toxotest, IDScreen, and Toxo Ab were 35.12%, 60.12%, 26.19%, and 10.12% respectively.  IDScreen had a good agreement with Toxo Ab (Gwet's AC1= 0.623) and a moderate agreement with Pastorex (Gwet's AC1= 0.494-0.511).  Toxotest had a low agreement with three commercial kits (Gwet's AC1= 0.2) but had a moderate agreement with western blotting (WB) and modified agglutination test (MAT) (Gwet's AC1= 0.458-0.557).
DETERMINATION OF THE BEST ROSELLA (Hibiscus sabdarifa L.) FILTRATE CONCENTRATION IN EGG YOLK CITRATE DILUENT Arman Sayuti; Herdina Mayuri; Nuzul Asmilia; Tazul Arifin; Mulyadi Adam
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.427 KB) | DOI: 10.21157/j.ked.hewan.v15i4.22398

Abstract

The aim of this research was to determine if the substitution of rosella filtrate (Hibiscus sabdarifa L.) in citrate-based diluent and egg yolks with the ratio 4:1 (16 mL citrate + 4 mL yolk) would help maintain the progressive motility of Kacang goat spermatozoa at room temperature. This study used a male Kacang goat with an average sperm quality of approximately ≥ 205 x 107 spermatozoa/mL and motility of 81.6%. An electro-ejaculator was used once a week to collect the semen. The treatments were labelled as follows: P0 was the negative control group, treatment P1 was given 5% rosella filtrate, treatment P2 was given 10% rosella filtrate, and treatment P3 was given 15% rosella filtrate. Examination of the motility of the spermatozoa was carried out at room temperature. The examination was carried out once every four hours. It was concluded that the rosella filtrate could maintain the progressive motility of spermatozoa of the Kacang goat at optimal concentrations. The optimal motility percentage was found at a concentration of 15% at a storage life of 0-4 hours.
CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Harrold Halilintar MR Subu Taopan; Fitriya Nur Annisa Dewi; Silmi Mariya; Huda Darusman; Permanawati Permanawati; Dondin Sajuthi; Irma Herawati Suparto
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.996 KB) | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

 Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
ULTRASOUND IMAGING OF THE SPLEEN, STOMACH, LIVER, KIDNEYS, BLADDER, AND PROSTATE IN PIGLETS (Sus scrofa domestica) Gunanti Gunanti; Carina Khairunnisa; Fadhilah Nur Annisa; Elok Budi Retnani; Akhmad Arif Amin; Dwi Utari Rahmiati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.276 KB) | DOI: 10.21157/j.ked.hewan.v15i4.21784

Abstract

This study aims to describe ultrasonographic imaging of the spleen, stomach, liver, kidney, bladder, and prostate in piglets (Sus scrofa domestica). Ultrasonography was performed on six male piglets aged 1 month with a body weight of 4-8 kg. Ultrasonography of the spleen, stomach, liver, urinary bladder, and prostate was observed in the dorsal-recumbency position, and the kidney was observed in the lateral-recumbency position. The ultrasound examination used the Chison EBit 60, which is a linear type transducer with a frequency of 9 MHz, transverse and sagittal positions, and a gain of about 55-80. Ultrasonography of the spleen showed that the shape of the spleen head varied in each individual. The results of gastric ultrasonography showed the gastric lining clearly and the similarity of the position, shape, structure, echogenicity, and size of the stomach. The results of liver ultrasonography showed that the echogenicity of the liver tended to be hyperechoic, the kidneys were hyperechoic, and the bladder was hypoechoic due to the use of improper gain. The average length of the right kidney was 4.73±0.50 cm and the left kidney was 4.08±0.26 cm. The bladder wall thickness was 1.3±0.31 mm. Imaging of the bladder showed changes in wall size and echogenicity. Prostate ultrasound results obtained an average length of 2.68±0.45 cm, width 1.69±0.35 cm. The results of this study can be used as a reference for normal data on organ position, organ structure, and organ echogenicity of the spleen, stomach, liver, kidney, bladder, and prostate in piglets.
CAUSES AND REMEDIES FOR NON-INFECTIOUS INFERTILITY IN SHEEP AND GOATS Ömer Uçar; Baris Atalay Uslu
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.654 KB) | DOI: 10.21157/j.ked.hewan.v15i4.20795

Abstract

Non-infectious infertility is particularly critical for sire rams/bucks, as seasonal breeders. This seasonal heavy use of males may lead to sexual exhaustion such that the regular semen production, libido, ejaculation, and litter yield can be seriously impaired. In severe cases, the reproductive processes may stop completely. For avoiding infertility risk, management and feeding should firstly be arranged with a flock-based approach. In reproductive management, the issues of using teaser ram/buck, male/female ratio, weekly mating number, exercise, and experience need attention. Also, inbreeding and using old breeders should be avoided. In oestrus synchronisation, necessary precautions against extremely hot/arid climatic conditions should be considered together with a sufficient number of rams/bucks used rotationally. For sustainable sheep/goat breeding, protective-preventive measures are preferred to problem-solving approaches. In enterprises where female breeder welfare is a priority, sustainable individual yield increases. Further, only the comfort level of males used heavily in season allows the expected libido and fertility to occur. In small ruminants, the necessary interest and responsibility in production provide convenience in the ideal solution with the opportunity of early interventions against undesirable fertility problems in breeders. Undoubtedly, it is vital to consider sustainable animal health and welfare for profitable breeding and husbandry in sheep/goats.
THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES Dona Astari Nurkarimah; Ekayanti Mulyawati Kaiin; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.47 KB) | DOI: 10.21157/j.ked.hewan.v15i4.17688

Abstract

The purpose of this study was to evaluate the effectiveness of cysteamine supplementation on the maturation medium and/or in vitro fertilization medium with regards to improving the normal fertilization rate of sheep oocytes, which are characterized by the formation of two pronuclei. Grade A and B oocytes were matured in medium-199 with 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), 10 IU/mL human chorionic gonadotrophin (hCG), and 50 μg/mL gentamicin added for 24 hours in a 5% CO2 incubator at 39° C. The treatment group was divided into the following groups: a control group with no cysteamine (P1), a group with 100 μM cysteamine in the maturation medium (P2), a group with 100 μM cysteamine in the fertilization medium (P3), and a group with 100 μM cysteamine both in the maturation and fertilization medium (P4). The fertilization was carried out by incubating sperm-oocytes for 12 hours and then staining them with 1% aceto-orcein to observe the formation of a pronucleus. Normal fertilization rates obtained by each treatment group were 56.5% (P1), 57.1% (P2), 57.8% (P3), and 59.9% (P4) with no significant difference (P0.05) between groups. It was concluded that 100 μM cysteamine supplementation in both the maturation medium and fertilization medium was not able to increase the normal fertilization rate of sheep oocytes.
DETERMINATION OF THE BEST ROSELLA (Hibiscus sabdarifa L.) FILTRATE CONCENTRATION IN EGG YOLK CITRATE DILUENT Arman Sayuti; Herdina Mayuri; Nuzul Asmilia; Tazul Arifin; Mulyadi Adam
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.22398

Abstract

The aim of this research was to determine if the substitution of rosella filtrate (Hibiscus sabdarifa L.) in citrate-based diluent and egg yolks with the ratio 4:1 (16 mL citrate + 4 mL yolk) would help maintain the progressive motility of Kacang goat spermatozoa at room temperature. This study used a male Kacang goat with an average sperm quality of approximately ≥ 205 x 107 spermatozoa/mL and motility of 81.6%. An electro-ejaculator was used once a week to collect the semen. The treatments were labelled as follows: P0 was the negative control group, treatment P1 was given 5% rosella filtrate, treatment P2 was given 10% rosella filtrate, and treatment P3 was given 15% rosella filtrate. Examination of the motility of the spermatozoa was carried out at room temperature. The examination was carried out once every four hours. It was concluded that the rosella filtrate could maintain the progressive motility of spermatozoa of the Kacang goat at optimal concentrations. The optimal motility percentage was found at a concentration of 15% at a storage life of 0-4 hours.
CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Harrold Halilintar MR Subu Taopan; Fitriya Nur Annisa Dewi; Silmi Mariya; Huda Darusman; Permanawati Permanawati; Dondin Sajuthi; Irma Herawati Suparto
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

 Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
ULTRASOUND IMAGING OF THE SPLEEN, STOMACH, LIVER, KIDNEYS, BLADDER, AND PROSTATE IN PIGLETS (Sus scrofa domestica) Gunanti Gunanti; Carina Khairunnisa; Fadhilah Nur Annisa; Elok Budi Retnani; Akhmad Arif Amin; Dwi Utari Rahmiati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.21784

Abstract

This study aims to describe ultrasonographic imaging of the spleen, stomach, liver, kidney, bladder, and prostate in piglets (Sus scrofa domestica). Ultrasonography was performed on six male piglets aged 1 month with a body weight of 4-8 kg. Ultrasonography of the spleen, stomach, liver, urinary bladder, and prostate was observed in the dorsal-recumbency position, and the kidney was observed in the lateral-recumbency position. The ultrasound examination used the Chison EBit 60, which is a linear type transducer with a frequency of 9 MHz, transverse and sagittal positions, and a gain of about 55-80. Ultrasonography of the spleen showed that the shape of the spleen head varied in each individual. The results of gastric ultrasonography showed the gastric lining clearly and the similarity of the position, shape, structure, echogenicity, and size of the stomach. The results of liver ultrasonography showed that the echogenicity of the liver tended to be hyperechoic, the kidneys were hyperechoic, and the bladder was hypoechoic due to the use of improper gain. The average length of the right kidney was 4.73±0.50 cm and the left kidney was 4.08±0.26 cm. The bladder wall thickness was 1.3±0.31 mm. Imaging of the bladder showed changes in wall size and echogenicity. Prostate ultrasound results obtained an average length of 2.68±0.45 cm, width 1.69±0.35 cm. The results of this study can be used as a reference for normal data on organ position, organ structure, and organ echogenicity of the spleen, stomach, liver, kidney, bladder, and prostate in piglets.
CAUSES AND REMEDIES FOR NON-INFECTIOUS INFERTILITY IN SHEEP AND GOATS Ömer Uçar; Baris Atalay Uslu
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.20795

Abstract

Non-infectious infertility is particularly critical for sire rams/bucks, as seasonal breeders. This seasonal heavy use of males may lead to sexual exhaustion such that the regular semen production, libido, ejaculation, and litter yield can be seriously impaired. In severe cases, the reproductive processes may stop completely. For avoiding infertility risk, management and feeding should firstly be arranged with a flock-based approach. In reproductive management, the issues of using teaser ram/buck, male/female ratio, weekly mating number, exercise, and experience need attention. Also, inbreeding and using old breeders should be avoided. In oestrus synchronisation, necessary precautions against extremely hot/arid climatic conditions should be considered together with a sufficient number of rams/bucks used rotationally. For sustainable sheep/goat breeding, protective-preventive measures are preferred to problem-solving approaches. In enterprises where female breeder welfare is a priority, sustainable individual yield increases. Further, only the comfort level of males used heavily in season allows the expected libido and fertility to occur. In small ruminants, the necessary interest and responsibility in production provide convenience in the ideal solution with the opportunity of early interventions against undesirable fertility problems in breeders. Undoubtedly, it is vital to consider sustainable animal health and welfare for profitable breeding and husbandry in sheep/goats.

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