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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 402 Documents
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Utilization of thermophilic cellulolytic bacteria isolates and Trichoderma harzianum fungi on rice strow composting Dini Mufriah; Rini Sulistiani
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The thermophilic cellulolytic bacteria are potential organisms for applications such as in decomposition of organic materials and enzyme technology. One potential organic materials which is very abundant in Indonesia is rice straw, which have not been optimally utilized. The problem is, the compostingprocess of straw in nature is used to take along time, so it needs to add effective decomposer microoroganisms which can adapt on compost termperature rise. The aims of this research were to isolated and selected thermophilic cellulolytic bacteria and Trichoderma harzianum and tested the isolates on rice straw composting. This research is executed in laboratory in faculty of agriculture, University of Alwashliyah Medan. Twenty thermophilic cellulolytic bacteria were isolated from several process composting of rice strawand corn waste at high temperature, from peat land and sidebu-debu hot spring, while Trichoderma harzianum fungi that used in this research was already got from previous research. Cellulolytic activity was determined by ability of bacteria and fungi to degrade carboxymethyl cellulose (CMC) substrate. Cellulolytic microorganisms was selected based on clear zone which form surrounding the colonies. Cellulase activity was measured by dinitrosalisilic acid (DNS) method. Results showed that there were 5 isolates (JG12, JG9,JG7 JR2, SD1) showing the best ability to degrade CMC. JG12 isolate had the higest diameter ratio of clear zone and enzyme activity ( 2,6 and 5,07 x 10-3 U/mL), while T. Harzianum had ratio of clear zone 1,22 and enzyme activity 4,02 x 10-3 U/mL).Then the selected bacteria and T. Harzianum were test on rice straw composting. This selection was used complete block Random Design Factorial, which consist of bacteria isolates as the first factor (S0=control, S1= Straw+JG7, S2=Straw+JR2, S3=Straw+JG9, S4=Straw+JG12, S5=Straw+SD1) and T. Harzianum as the second factor( T0=control, T1=Straw+T. harzianum). The result of this research are the highest decreasing of ratio C/N is caused by the treatment combination S4T1 which is consist of JG12 bacteria isolate that isolated from corn waste composting and Trichoderma harzianum (C/N = 17,16).
In vitro antioxidant activity of Lantadene A Chong Grace-Lynn; Ibrahim Darah; Sasidharan Sreenivasan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Lantadenes are the pentacyclic triterpenoids present in the leaves of the plant Lantana camara. Pentacyclic triterpenes are often studied as their biological properties are considerable and contributes to the development of modern therapeutic drugs. In recent years various natural product based compounds are extensively studied for various pharmacological activities including antioxidant activity. Therefore, this study aims to determine the in vitro antioxidant activity of Lantadene A (from Lantana camara ) as results will enhance the knowledge of the compound towards development of hepatoprotective agents. The antioxidant assays performed on the compound were the DPPH radical scavenging assay, nitric oxide assay, superoxide anions scavenging activity and iron chelating assay. Results showed promising antioxidant activities as the IC50 for Lantadene A in the above assays were 6.574 mg/ml, 0.098 mg/ml, 2.506 mg/ml and 0.001 mg/ml respectively as compared to the standards (BHT, ascorbic acid and EDTA) 0.027 mg/ml, 0.075 mg/ml, 1.025 mg/ml and 0.47 mg/ml respectively. The DPPH radical scavenging assay for Lantadene A was weaker than BHT while iron chelating assay of Lantadene A is much stronger than the standard. The rest of the assays were comparable to the reference standards. These results suggest that Lantadene A can be a potential cadidate to be developed as an antioxidant. 
Correlation of streamflow and sediment concentration at upstream and downstream Padang Watershed Kemala Sari Lubis; Erwin Masrul Harahap; Abdul Rauf; Zainal Arifin Hasibuan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 2 (2013): Engineering
Publisher : Syiah Kuala University

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Abstract

Sediment concentration affected b streamflow at river body. This research was conducted to study correlation between streamflow and sediment concentration at upstream and downstream Padang watershed on July until December 2013. The research was conducted by using survey method at two outlet of Padang watershed were Padang sub watershed and Padang Hilir sub watershed. Sampling of water at two (2) outlets were conducted at stream surface by using depth intergrating suspended sediment sampler technique at three (3) points of river body. Sampling waters conducted at two times that were after rainand no rain. Sediment concentration (Cs) calculated by Cs = (G2 – G1) / V equation. Streamflow (Q) calculated by multiplying velocity and wide of river by Q = A x V equation. The results showed that no correlation between streamflow and sediment concentration at upstream when no rain (Cs = 0,0007 +0,0412 Q; R2 = 0,43) and at downstream when no rain (Cs = 0,0002 Q + 0,07157; R2 = 0,057). There were correlation between streamflow and sediment concentration at upstream after rain (Cs = 0,00041 Q +0,0611; R2 = 0,88) and at downstream after rain (Cs = 0,00015 Q + 0,03293; R2 = 0,9).
Inflammation and the Migration of Mesenchymal Stem Cell Reza Maulana; Nur Wahyuniati; Imai Indra
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Mesenchymal stem cells are adult non-hematopoietic stem cells with multilineage proliferation and differentiation capabilities. This type of stem cell has the multipotent ability to differentiate into osteocytes, tenocytes, adipocytes, chondrocytes and  bone marrow stromal cells. The migration mechanism of mesenchymal stem cell is not yet fully understood, but based on studies  that  have  been  done  recently  by  the  researchers  worldwide  shown  that  the inflammatory process plays an important role for mesenchymal stem cell migration. A number of chemokine that plays a role in the migration of mesenchymal stem cells such as MCP-1 (CCL2), CXCL8, RANTES (CCL5), LL-37, integrin β1, CD44 receptor, CCR2, CCR3, and tyrosine kinase receptors for the following growth factors: IGF -1, PDGF-bb, HGF and VEGF
Analysis for ground anchor using 2D simulation model by MIDAS/GTS software MUNIRA SUNGKAR; JASON WU
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 1 (2014): Engineering
Publisher : Syiah Kuala University

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Abstract

Ground anchors are commonly exposed to both acidic and alkaline ground conditions depending on ground mineral content, soil/rock type, age and groundwater flow path. It is essential to understand the limitations to ground anchorage materials especially when exposed to aggressive ground conditions whilst in a stressed state. This study discusses about ground anchors and analysis of the ground anchor failure trigger by corrosion, which analysed by using difference software in previous study (ABAQUS) compare with simulation model analysed by 2D Midas/GTS. Numerical techniques may be very efficient for investigating the load transfer of ground anchors. The results of anchor failures in numerical modeling are different from previous study (field test results), practiced by first cement crack and then wire break. Recommendations are given for applying FEM (finite element method) analysis to the design of anchored stabilization systems, and also for analyzing design variables, and design loads, to achieve more efficient and cost-effective anchored stabilization systems. Several models or simulations are presented to illustrate the displacement and the load that applied to the anchor which analyzed by Midas/GTS software. However, the condition that the numbers of occurrence correspond to load of failures at the time of damages is caused as the consequence of material and model deviation
Effect of moisture content and amount of Hexane on in-situ transesterification of jatropha seeds for biodiesel production Ryan Moulana; . Satriana; M. Dani Supardan; Wirda Aina
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 2 (2013): Engineering
Publisher : Syiah Kuala University

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Abstract

The aim of this research is to study in-situ transesterification technique of jatropha seeds for biodiesel production. This research was conducted using a randomized factorial block design consisting of two factors that is moisture content of jatropha seeds (2% and 3%) and amount of hexane that used as a co-solvent (0 ml, 75 ml, 95 ml, 115 ml and 135 ml). The experimental result shows that the highest yieldbiodiesel of 78.72 % obtained at process condition of jatropha seeds moisture content of 2% and amount of hexane of 115 mL. The physico-chemical analysis of biodiesel procured the acid number of 0.35 mg KOH/g, density of 0.89 g/mL and viscosity of 3.04 mm2/ s that meet requirement of Indonesian Nasional StandardNo. 04-7182-2006.
Study of Digestion Efficiency on Spectrophotometric Determination of Phosphate In Water Sample by Flow Injection Analysis . Lelifajri
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
Publisher : Syiah Kuala University

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Abstract

A method suitable for the determination of phosphate in water samples is presented. The flow injection (FI) manifold contain a PTFE reaction coil wrapped around a low power UV lamp and is based on the spectrophotometric determination of Orthophosphate and mineralized organic phosphate at 690 nm after reduction of phosphomolybdate to molybdenum blue with tin(II) chloride. The linear range was 0-1.5 mg L-1 P, with a detection limit (3 s) of 7 μg L-1 and a sample throughput of 40 h-1. The tolerance of interference from Fe(II), Al(III), and Fe(III) was achieved using on-line sample pre-treatment by a strong acid ion exchange column
Regeneration rates of Dendrobium bobby messina plbs with ascorbic acid using PVS2 vitrification Jessica Jeyanthi James Antony; Chan Lai Keng; Sreeramanan Subramaniam
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Cryopreservation techniques using PVS2 vitrification was applied on PLBs of Dendrobium Bobby Messina, with survival monitored through observations of growth rate and the 2,3,5-triphenyltetrazolium chloride (TTC) analyses. The parameters optimized were PLBs size, preculture concentration, preculture duration, PVS2 incubation temperature and duration. The optimized parameters obtained were 3-4mm of PLBs precultured in 0.2M sucrose for 1 day, treated with a mixture of 2M glycerol and 0.4M sucrose supplemented with half strength liquid MS media at 25°C for 20 minutes and subsequently dehydrated with plant vitrification solution 2 (PVS2) at 0°C for 20 minutes prior storage in liquid nitrogen. Following rapid warming in a water bath at 40°C for 90 seconds, PLBs were washed with a half strength liquid MS media supplemented with 1.2M sucrose. Subsequently, PLBs were cultured on half strength semi-solid MS media supplemented with 2% (w/v) sucrose in the absence of growth regulator. The optimized vitrification method was successful in preserving this orchid as it produced growth recovery in cryopreserved PLBs up to 40%. Ascorbic acid was added in the media to evaluate the regeneration process of cryopreserved PLBs. However, growth recovery rate was only 10% at 0.6mM ascorbic acid. RAPD analysis using 6 primers indicated that cryopreserved and non-cryopreserved PLBs from vitrification method were genetically faithful to the mother plant. However, 3 primers showed polymorphism and 1 primers indicated partial polymorphism between the cryopreserved and non-cryopreserved PLBs in comparative to the mother plant.
Tidal analysis at Kuala Langsa and Pusong Island using Admiralty method ANNISA S. SUGIARTI; . MARWAN; ICHSAN SETIAWAN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 1 (2014): Engineering
Publisher : Syiah Kuala University

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Abstract

A research about tidal analysis at Kuala Langsa and Pusong Island with different physical condition had been done, where Kuala Langsa is an estuary and Pusong Island is a coastal area in Kota Langsa District, Aceh Province. The purpose of this research is to determine tidal type on both locations. This research used secondary data which is obtained from Kuala Langsa – Pusong Island Hydro – Oceanographic Survey and primary data which is taken about 29 hours at both locations.  Admiralty method had been using to analyze the tides and from this method can be obtain amplitude and phase lag based on Formhazl number. The result of this research according to Formhazl number for Kuala Langsa and Pusong Island are 0.12 and 0.23 respectively. Both values of Formhazl number of the locations are smaller than 0.25, it concluded that both locations had showed same tidal type, i.e. semidiurnal. The phase lag for tidal types in Kuala Langsa for M2 is 3590while for Pusong Island is 3300. While for the amplitude of both locations for M2 is 56 cm for Kuala Langsa and 48 cm for Pusong Island. It is shown that the amplitudes at Kuala Langsa are a little bit higher than that of Pusong Island. The difference is suspect due to the different physical condition of the observation areas
Morphology and Parasitaemia Development of Plasmodium berghei in Balb/c Mice (Mus musculus) Rosnizar Rosnizar; Kartini Eriani
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Malaria is one of the most severe public health problems worldwide. It is a leading cause of death and disease in many developing countries, where young children and pregnant women are the groups most affected. Malaria disease caused by Plasmodium parasite have symptoms that typically include fever, fatigue, vomiting and headaches. In severe cases, it can cause yellow skin, seizures, coma or death. The present study is aimed to monitoring parasitemia level and percentage of parasite morphology as parasitaemia progresses. This research used Plasmodium berghei NK strain obtained from National University of Malaysia which originally from MR4, USA. Design used in this research was completely randomized design, with 2 treatments which were mice without infection and mice with infection of P. berghei parasite. Method used in this research was staining method of thin smear of blood using Giemsa stained and observed by microscopic. Parameter of observation were morphology and development of P. berghei at different level of parasitaemia (10%, 20%, 30% and 40%). The result showed from microscopic examination of blood slides prepared from the study animals indicated that the ring form was dominant stage obtained at all different stages of infection followed by trophozoite stage. Meanwhile schizont stage was the lowest stage obtained at all different stages observed.

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