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INDONESIA
LenteraBio
Published by Universitas Negeri Surabaya
ISSN : 22523979     EISSN : 26857871     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 281 Documents
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PENINGKATAN PATOGENESITAS VIRUS LATEN HASIL ISOLASI DARI KULTUR SEL MIDGUT LARVA SPODOPTERA LITURA TERHADAP LARVA SPODOPTERA LITURA MELALUI PENGINFEKSIAN BERULANG IQBAL FILAYANI, MUHAMMAD Iqbal
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Nuclear polyhedrosis virus (NPV) is a virus of the family Baculoviridae that attack Lepidoptera. Nuclear polyhedrosis virus (NPV) were attacked S. litura called Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV). SlNPV Propagation can be done by multiplication of the host's body (in vivo) and in the larval midgut cell cultures S. litura (in vitro). Propagation in cell culture midgut expressed latent virus generated from midgut cells S. litura. Latent virus is inactive virus in cell larval Latent virus has a very low pathogenicity. This study aimed to improve latent viral pathogenicity with recurrent infection at larval S. litura by in vivo. This research used larval S. litura second instar with 200 larval S. litura and latent virus concentration used 7.8 x107 PIBs/ml. is the results showed that the latent virus pathogenicity  increase if infection done repeatedly, the first infection gain mortality by 14%, the second infection gain  mortality by 63%, the third infection gain mortality by 90%.
PEMBUATAN KOMPOS BERBAHAN BAKU LIMBAH LUMPUR PABRIK KERTAS DENGAN PENAMBAHAN TRICHODERMA HARZIANUM FITRI, INAYAH
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Sludge in the paper mill is now large. Utilization of sludge as composting is one alternative to use it. But the problems and constraints faced is the composting process takes about 3 months. In order to speed up the composting process, the addition of fungus T. harzianum is one of the alternative. The purpose of this study was to describe the quality of the nutrient concertation of sewage sludge compost from paper mill with the addition of T. harzianum as N, P, K, and C/N ratio. Descriptive data were analyzed by comparing the nutrient content and the soil criteria based on Hardjowigeno. The results showed that the addition of T. harzianum affected the quality of the nutrient of N, P, K and C/N ratio in compost. The concentration of N, P, K, and C/N ratio were 1.09% (very high), 0.24% (medium), 0.28 % (low) and 17 (high).
PENGARUH KIAMBANG (SALVINIA MOLESTA) YANG DIFERMENTASI DENGAN RAGI TEMPE SEBAGAI SUPLEMEN PAKAN TERHADAP PENINGKATAN BIOMASSA AYAM PEDAGING ZAMAN, QOMARUZ
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

This study aimed to determine the dose of fermented kiambang as feed supplements for increasing biomass of broiler; to define the number of consumption of feed boiler; and to determine feed conversion number caused by kiambang consumption. This research was experimental research with Completely Randomized Design (CRD) using fermented kiambang concentration as experimental factor. Kiambang was given using 6 kinds of concentrations: 0%, 10%, 20%, 30%, 40%, and 50% for 4 times repetition at random design. The data including increase of biomass boiler, feed consumption data, and conversion feed data, tested using Analysis of Variant and continued by Duncan?s Multiple Range Test (DMRT) 5% if the first test result was significant. Based on the increase of biomass boiler, feed consumption, and feed conversion, the 50% fermented kiambang concentration showed the best result to increase boiler biomass. These results suggested that kiambang fermentation can increase broiler biomass, reduce the number of feed consumed, and feed conversion.
PENGARUH PEMBERIAN DETERJEN TERHADAP MORTALITAS IKAN PLATY SP. KAMISWARI, RIZKY
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

One of the causes of water pollution are domestic wastes in the form of residual detergent. In the waste surfactants contain chemicals that are more resistant and does not change in a variety of media. Antiseptic ingredient in soap or detergent can disrupt the microorganisms, which is in the nature of water can be dangerous. This study aimed to determine the effect of detergent on Platy Sp. fish mortality at doses of 0 ppm, 18 ppm, 36 ppm, and 54 ppm of deterjent for 4 days treatment. The method used in this study was experimental design. The result of mortality obtained were analyzed using descriptive  analysis. From the research it could be concluded that the concentration of detergent affect the rate of mortality in Platy Sp. fish positively. At 18 ppm detergent was the mortality of  Platy Sp. longer than the mortality of the concentration at 54 ppm detergent.
UJI KEEFEKTIFAN CENDAWAN LECANICILLIUM MUSCARIUM DALAM MENGHAMBAT PERTUMBUHAN CENDAWAN PATOGEN TULAR TANAH (FUSARIUM OXYSPORUM, RHIZOCTONIA SOLANI, SCLEROTIUM ROLFSII) SECARA IN VITRO CAHYADI, ABDURRAZAK
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

One of the obstacles to the improvement and stabilization of crop production in Indonesia is soil borne disease pathogens that cause severe damage to agricultural crops in general and reduce both the quantity and quality of production. Three types of soil borne fungal pathogens commonly found in plants are F. oxysporum, R. solani and S. rolfsii, which resulted in the emergence of the fungus to soil borne diseases in crops such as corn, soybeans, potatoes, tomatoes, beans, cabbage, cucumber, cotton, and peanuts. This study aimed to describe the effectiveness of inhibiting fungus L. muscarium to inhibit the growth of F. oxysporum, R. solani and S. rolfsii in vitro. The experiment was conducted at the Laboratory of Microbiology, University of Surabaya C9 Building in October to December 2012. This study used the technique of exploratory observations made by growing the fungus in PSA medium for 5 days and then calculate the rate of growth of each fungus and calculate the inhibition of the fungus L. muscarium the three types of fungal pathogens. The results obtained were analyzed using descriptive quantitative analysis. From the results of this study concluded that the fungus L. muscarium has the effectiveness of different inhibitors on the three types of fungus pathogen (F. oxysporum, R. solani and S. rolfsii). The growth rate of each fungus did not show any difference. The fungus L. muscarium have rates similar maximal inhibitory effectiveness against fungus F. oxysporum and fungus R. solani by 66.31% and has a mean minimal inhibitory effectiveness against fungus S. rolfsii by 58.69%.
PENGARUH PENAMBAHAN DAUN LAMTORO TERHADAP KUALITAS KOMPOS KERTAS-LAMTORO DAN PEMANFAATANNYA TERHADAP PERTUMBUHAN TANAMAN BAYAM MERAH ZULIYANTI NINGSIH, RINA Zuliyanti
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

One alternative to use the sludge paper mill in order not to cause pollution in the environment is to use it as compost. Paper-lamtoro compost is expected to increase the levels of nutrients content, especially nitrogen (N) level. The purpose of this study were (1) to describe the quality of paper-lamtoro compost in the levels of N, P, K and C/N ratio and (2) to describe the effect of the concentrations of paper-lamtoro compost on the growth of red spinach plants. The study consisted of two phases, the first was the composting process and the second was the utilization of paper-lamtoro compost on plant growth red spinach plants performed experimentally using Randomized Block Design with one factor treatment, the concentration of compost includes 4.30 g, 6.45 g, 8.60 g, 10.45 g and 0.20 g of urea as a control. These treatment were repeated five times to obtain 25 samples. The parameters were the levels of N, P, K and C/ N ratio and the growth of the red spinach plants which includes the number of leaves, plant height and wet plant biomass. The first stage of data analysis was performed by comparing the descriptive criteria for nutrient content of the soil by Hardjowigeno (2003), and the second stage using analysis of variance (ANOVA), followed by LSD test to determine differences between treatments. The results showed that (1) the addition of Leucaena glauca leaves affect the quality of nutrient content of  N, P, K and C/ N ratio of paper-lamtoro compost produced. The level of N, P, K and C/ N ratio were 2.14%; 0.35%, 0.71% and 12.80% respectively (2) the concentration of paper-lamtoro compost affect significantly on the growth of red spinach plant. The best growth was found in the concentration of 10.75 g compost/ 3000 g planting medium.
PENGARUH PEMBERIAN FILTRAT DAUN KATUK TERHADAP KONSENTRASI DAN MORFOLOGI NORMAL SPERMATOZOA MENCIT (MUS MUSCULUS) YANG TERPAPAR ASAP ROKOK RATNA MUSTIKASARI, DWI Ratna; Tjandrakirana, Tjandrakirana
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

This research was carried out to prove that the katuk leaves filtrate can improves sperm quality and the concentration of malondialdehyde (MDA) as an indicator of free radicals is lower. The subjects of this reaserch were 60 male mice that were divided into 5 groups; Ko (normal control), K1 (exposured cigarette smoke control), P1 (exposed to cigarrete smoke and given katuk leaves filtrate 2 ml/100 gBW/day), P2 (exposed to cigarette smoke and given katuk leaves filtrate 2.5 ml/100 gBW/day), and P3 (exposed to cigrette smoke and given katuk leaves filtrate 3 ml / 100 gBW/hari). The results showed that katuk leaves filtrate can increase (P <0.05) the concentrations (> 80 million / ml) and normal morphology but the wasn?t difference (P <0.05).
UJI KEMAMPUAN PARASITIK JAMUR LECANICILLIUM LECANII TERHADAP MORTALITAS SISTA NEMATODA SISTA KUNING (GLOBODERA ROSTOCHIENSIS W.) NOHAN REMBULAN, SOLICHAH Nohan
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Golden Cyst Nematode is one of the nematode Tylenchida ordo with Heteroderidae family destructive and deadly plant potatoes in large numbers in cycles cyst. It can reduce potato crop productivity. Therefore, it is necessary to apply biological control (IPM) to control cyst Golden Cyst Nematode, one which is to use biological agents. One of those attempt is by using entomopatogenic fungi, for example is Lecanicillium lecani. This research is a preliminary study which aims to determine the ability of parasitic fungus L. lecanii against cyst of Golden Cyst Nematode at various concentrations in laboratory scale. This research was experimental type using Completely Randomized Design (CRD) with one treatment factor. The treatment factor was the concentration of L. lecanii, which consisted of 2 concentrations. They were 107 spores/ml108 spores/ml, and control (no fungus L. lecanii conidia) was repeated 6 times replications for a total of 18 experiment units. Each treatment unit used 10 cyst of Golden Cyst Nematode. The data obtained were the amounts of cyst mortality of Golden Cyst Nematode. It was observed until 2 weeks or the cyst of Golden Cyst Nematode covered by fungi. The data were analyzed by probit regression analysis using SPSS 16.0 for windows to get the percentage mortality of cyst of Golden Cyst Nematode and to know the LD80. Based on the results of the study, it can be concluded that the effectiveness of the parasitic fungus L. lecanii causing mortality cyst of Golden Cyst Nematode at concentration 108 conidia/ml.. The value of LD80 was get at concentration 1,4 x 108 spores/ml.
IDENTIFIKASI RHIZOBAKTERI PADA SEMANGGI (MARSILEA CRENATA PRESL.) YANG TERPAPAR LOGAM BERAT TIMBAL (PB) ARRIZAL, SYAFRUDDIN
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 1 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

The pollution of plumbum in Benowo, Surabaya cause the decrese of water quality. The objective of this research were to identification and to know characteristic of rhizobacteria in Marsilea crenata?s roots in the farm of  Benowo, Surabaya that flated plumbum 93.61mg/Kg. This research was descriptive research. The sample were 10 gram Marsilea crenata?s roots took from Benowo farm. The observation parameter were colony morphology, gram coloration, and characteristic of biochemistry test with Microbact Identification System. The identification of  rhizobacteria isolate  with Microbact Software dan buku Cowan and Steel?s Manual for Identification of Medical Bacteria Third Edition and Bargey?s Manual of Determinative Bacteriology Eight Edition. Data were analyzed descriptive qualitatively. The results indicated that characteristic and identification isolate, there are three bacteries, Streptococcus agalactiae; Bacillus circulans and Xanthobacter autrophicus with similary koefisiens is 90,5%, 88,2% and 81,5%.
KEKAYAAN JENIS KELELAWAR (CHIROPTERA) DI KAWASAN GUA LAWA KARST DANDER KABUPATEN BOJONEGORO Nurfitrianto, Hendrik; Budijastuti, Widowati; Faizah, Ulfi
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Bats are one of the animals that have the 2nd greatest diversity of species in the class of mammals after Rodentia. One of bats habitat is karst cave. East Java has more karst area like Tuban, Gresik and Bojonegoro. The bats of Lawa Cave at karst Dander have not studied yet. This study aimed to identify the species of bat in area Lawa Cave karst region Dander Bojonegoro. This research conducted during the months of February-March 2013. Bats collected directly from 4 stations in Lawa cave area using traps mistnet with size 3x10 m. Identification was done in the Taxonomy Laboratory Department of Biology UNESA. The results of this study obtained 5 species of bat: Rousettus amplexicaudatus, Hipposideros larvatus, Pipistrellus javanicus, Rhinolophus pusillus, Megaderma spasma.

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