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INDONESIA
Jurnal Matematika & Sains
Published by Institut Teknologi Bandung
ISSN : -     EISSN : -     DOI : -
Core Subject : Education,
Mathematics
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Articles 7 Documents
 1 
Search results for , issue " Vol 8, No 4 (2003)" : 7 Documents clear
Effect of 2,4-D on Indirect Somatic Embryogenesis and Surface Structural Changes in Garlic (Allium sativum L.) cv. Lumbu Hijau Totik Sri Mariani; Hiroshi Miyake; Rizkita Rachmi Esyanti; Ida Nurwendah
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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Abstract

A study concerning the effect of 2,4-D on indirect somatic embryogenesis and surface structural changes in garlic (Allium sativum L.) cv. Lumbu Hijau was conducted. The purposes of this study were to evaluate the effect of 2,4-D on the induction of somatic embryogenesis and to observe the developmental stage of somatic embryo as well as the surface structural changes of somatic embryo in garlic. Root tips explants were cultured on embryogenic callus induction medium (ECIM). Somatic embryos were then transferred to the embryo maturation medium (EMM), desiccated and subsequently transferred to the repetitive somatic embryogenesis medium (RSEM). Observation by dissecting microscope showed that embryogenic callus and somatic embryo was formed at ECIM containing 0.1 μM 2,4-D. At EMM containing 0.01 μM 2,4-D somatic embryo developed into mature somatic embryo. Somatic embryo underwent repetitive somatic embryogenesis that consisted of globular and mature somatic embryo at RSEM, without plant growth regulator. Observation by scanning electron microscopy showed that the development al stages of garlic somatic embryo consisted of proembryo, transition phase from proembryo to globular, globular, mature embryo and single cotyledon stages. Effect of 2,4-D on the cell surface structure could be seen on each somatic embryo developmental stage. In ECIM containing 0,1 μM 2,4-D, proembryo, transition embryo and globular embryo were formed. The surface of proembryo was smooth whereas fibrillar material as cellulose microfibril, was observed during the transition from proembryo to globular stage. The surface of globular stage was entirely covered by a new cell wall. Subsequently, in 0.01 μM 2,4-D, mature embryo and single cotyledon were formed, which their cell wall surface was stable. From this study, it could be concluded that the optimum concentration of 2,4-D for induction of embryogenic callus and initiation of somatic embryo was 0.1 μM. Decreasing of 2,4-D concentration to 0.01 μM resulted in the development of globular embryo to mature and single cotyledon stage. Repetitive somatic embryogenesis occurred in RSEM medium without plant growth regulator. Based on this result it can be concluded that each somatic embryo developmental stage had a typical characteristic of surface structure.
Peningkatan Produksi Azadirahtin dalam Kultur Suspensi Sel Azadirachta indica A.Juss melalui Penambahan Skualen Zulfa Zakiah; Erly Marwani; Arbayah H. Siregar
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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One of the methods to enhance the secondary metabolite content in plant tissue culture is precursor feeding. The study on azadirachtin enhancement in cell suspension culture of Azadirachta indica A.Juss was conducted by supplying squalene as precursor. Callus were induced on Murashige-Skoog solid medium with addition of 0,5μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 1,0μM 6-benzylaminopurine (BAP). Callus were subcultured to Murashige-Skoog liquid medium with addition of 0,1μM 2,4-D and 1,0μM BAP. The cell suspension cultures were subcultured three times, and then treated each with 10, 100, and 1000μM of squalene. Azadirachtin content was measured at 0, 2, 4, 6, 8, 10 and 12 days after feeding using HPLC (High Pressure Liquid Chromatography) with methanol:water (6:4) as mobile phase. The results showed that the highest azadirachtin content in cell suspension culture before supplying squalene was 0,041 g/g cell dry-weight (dw) at the age of 8 days culture. The increment of azadirachtin content in cell and medium was significantly influenced by squalene concentration and age of culture. The highest azadirachtin content after feeding in the cell mass (0,076 ± 0,006 g/g dw) and in the medium (0,229 ± 0,003 mg/L)was achieved on supplying 100μM squalene to 10 and 12 days culture.
Efek Antihipertensi dan Hipotensi beberapa Fraksi dari Ekstrak Etanol Umbi Lapis Kucai (Allium schoenoprasum L., Lliliaceae) Irda Fidrianny; Kosasih Padmawinata; Soediro Soetarno; Elin Yulinah
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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Hypotensive and antihypertensive effect of fractions of ethanolic extract of “kucai” (Allium schoenoprasum L., Liliaceae) bulb had been tested on male Wistar rats. The ethyl acetate, n-buthanol and aqueous fractions of both fresh and dry bulb had hypotensive and antihypertensive effects. The aqueous fraction of fresh bulb at a dose of 25 mg/kg body weight showed the highest hypotensive and antihypertensive activities. The n-buthanol fraction of dry bulb at a dose of 50 mg/kg body weight showed the highest hypotensive activity. The ethyl acetate fraction of dry bulb at a dose of 50 mg/kg body weight showed the highest antihypertensive activity.
Pengaruh Pelarut dan Temperatur terhadap Tranport Europium (III) melalui Membran Cair Berpendukung Buchari Buchari; Eti Testiati; Aminudin Sulaeman
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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Study of the influence of solvents and temperature on the transport of Eu(III) through supported liquide membrane (SLM) has been undertaken. The first step of this work was to determine the best conditions for the extraction. Polytetrafluoroethylene (PTFE) membrane has been used in this research. The thickness of the film is 47 μm and the average pore size is 0.45 μm.The membrane was impregnated in the carrier solutions for 2 hours. The carrier used were di 2-ethylhexyl phosphoric acid (D2EHPA) and tributylphosphate (TBP). Feed compartment contained 100 ppm Eu(III) in a buffer solution. Receiver compartment contained 0.1 M nitric acid. The optimum result has been achieved at feed pH of 3 and a mixture of 0.8 M D2EHPA and 0.2 M TBP in kerosene. At this conditions, 71.74 % of Eu( III) was transported through SLM. The transport percentase ncreased in the solvent order of kerosene>toluena>chloroform>n-hexane>1,2-dichloroethane. The mixture of 75% of kerosene and 25% of chloroform increased the Eu(III)’s transport from 71.74 % to 79.1% compared with the kerosen as a solvent. The elevation of temperature from 25oC to 60oC increased the transport of Eu(III) from 71.74 to 92.26 %. The profile of membrane surface was characterized by scanning electron microscope (SEM).
Pengaruh Variasi Komposisi Amilosa terhadap Kemudahan Biodegradasi Poliuretan Eli Rohaeti; N. M. Surdia; Cynthia L. Radiman; E. Ratnaningsih
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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Amylose had been used to synthesize polyurethanes. A mixture of polyethylene glycol (PEG) with a molecular weight 400 with amylose was reacted with diphenylmethane-4,4’-diisocyanate (MDI) at room temperature to produce polyurethanes. The precured polyurethanes were heat-pressed and PU sheets were obtained. Characterization of functional groups was determined by FTIR spectrophotometry and crystallinity was analyzed by using XRD. The hydrogen bonding index (HBI) of polyurethane samples increased with an increasing of amylose content. The addition of 15% (w/w) amylose decreased the degree of crystallinity of polyurethane. The films of polyurethanes were biodegraded by Pseudomonas aeruginosa in an liquid of Luria Bertani (LB) media at 37oC for 5, 10, 15, 20, 25, and 30 days, with the changing of media every 5 days. The result of weight loss percentace showed that all polymers may be biodegraded, with different biodegradation rate. The biodegradability (rate of weight loss) of polyurethanes by Pseudomonas aeruginosa increased when the amylose content in the polyurethane increased. The polyurethane product with 15% amylose – PEG400 – MDI had the highest biodegradability.
Struktur Lapis TitanoNiobat terpilarkan Polimer-{Ti(IV) Okso Hidroksida} Hari Sutrisno
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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The purpose of this research was to incorporate the cation of [Ti8O12(H2O)24]8+ in the interlayer of HTiNbO5 by so-called “Chimie Douce” method. The cation of [Ti8O12(H2O)24]8+ has been tranformed to polymer-{Ti(IV) Oxo Hydroxide} and pillared in the interlayer HTiNbO5. The incorporation of polymer-[Ti(IV) Oxo Hydroxide] in the interlayer HTiNbO5 has been confirmed by powder X-ray diffraction, Scanning Electron Microscopy (SEM) and High Resolution Transmission Electron Microscopy (HRTEM). The exchange of the polymer of {Ti(IV) Oxo Hydroxide} was facilitated by opened layers of butylamine-intercalated complexes.
Synthesis of Catalytically Active High Surface Area Sulfated Zirconia Totok E. Suharto
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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A new synthesis method to produce active sulfated zirconia catalyst has been developed. Sulfated zirconium oxide has been prepared by one-step synthesis, where the sulfatation was directly introduced during the gelating step of zirconium hydroxide. The gelating conditions and other synthesis parameters, such as drying and calcination procedures have important influence on the product properties. The obtained sulfated zirconia has high specific surface area, up to 180 m2g-1. The surface area of sulfated zirconia was influenced by increasing of pH-value of the zirconium hydroxide gel in a range of 5 to 9. Active sulfated zirconia catalyst could be prepared by optimalization of synthesis conditions and resulfatation. The catalytic activity of this catalyst in the isomerization of n-butane was higher than the activity of commercial sulfated zirconia and zeolite of mordenite type. The active sulfated zirconia showed a very high selectivity for iso-butane of 85 – 95%, whereas mordenite showed low selectivity of only 40 – 60%. The deactivated catalyst can be simply regenerated by calcination.

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