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Nexus Biomedika
Published by Universitas Sebelas Maret
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Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
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Articles 24 Documents
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Search results for , issue "Vol 3, No 3 (2014): Nexus Biomedika" : 24 Documents clear
Hubungan antara Yoga dengan Siklus Menstruasi Sari, Indah Purnama; Kiyatno, .; Hanim, Diffah
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Disturbed menstrual cycle often causes anxiety women. Menstruation disturbance can affect daily activities, that can decrease productivity. Nutrition and physical activities can affect regularity of menstrual cycle. Yoga is a popular physical activity recently that can influence hormone reproduction. This study aims to investigate the relationship between Yoga and menstrual cycle. Method: This research was an observational analytic research with cross sectional approach. There were 63 subjects of research that were chosen using purposive sampling of women who took Yoga course in Ganeps Surakarta. Independent variable of this research was Yoga. While dependent variable of this research was menstrual cycle. Data collected by direct interview with a list of questions to respondent were analyzed using bivariate Chi-Square, Contingency coefficient, and Odds. Result: The result of  Chi-Square test showed there was significant correlation between Yoga and menstrual cycle (p = 0.000). Coefficient of contingency test showed that 43.5% women who took Yoga course had regular menstrual cycle (C = 0.43; and p =0.000). While Odds Ratio test showed that women who took Yoga course had possibility 9.86 times to have regular menstrual cycle (OR = 9.86; CI 95% 2.87 - 34.95; and p = 0.000). Result of Research: There is significant correlation between Yoga and menstrual cycle. Keywords: Yoga, menstrual cycle 
Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris L.) terhadap Struktur Histologis Ginjal Mencit (Mus musculus) Akibat Paparan Minyak Kelapa Sawit Pemanasan Berulang Ramadhan, Fitri Febrianti; Muthmainah, .; Widyaningsih, Vitri
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: One of plants with abundant antioxidant compounds is red beet (Beta vulgaris L.). That antioxidant compounds can be found in betalain pigment, β-karoten, vitamin A, vitamin C, vitamin E, flavonoid, mineral Cu, Mn, Zn and selenium. This research was conducted to prove the nefroprotective effect and the optimal dose of ethanol extract of red beet which can prevent renal cells damage of mice induced by deep frying palm oil. Methods: This research was laboratory experimental research. Samples were thirty Swiss webster male mice, 2-3 months old and ± 20 grams of each weight divided into 5 groups randomly, each groups have six mice. Normal control group (KKn) wasn’t given any treatments, only aquades. The second group (KP) was induced 0.06 ml/ 20 g weight deep frying palm oil orally and aquades every day for 14 days that can cause renal cells damaging. KP1, KP2, and KP3 were given nephroprotector treatment of ethanol red beet extract with dosage 5.6 ml/ 20 g weight, 11.2 ml/ 20 g weight, and 22.4 ml/ 20 g weight and then after an hour induced 0.06 ml/ 20 g weight frying palm oil orally every day for 14 days. Renal histological imaging were assessed by the sum of the cell nucleus picnosis, cariorecsis, and cariolysis. Data were analyzed using One-Way ANOVA and followed by Post Hoc Multiple Comparisons test. (α = 0.05) Results: One-way ANOVA test showed that the value of p was < 0.001. Mean of each group were KKn (8.67±1.27), KP (44.63±1.31), KP1 (33.88±1.15), KP2 (22.75±1.70), and KP3 (12.79±1.38). Post hoc multiple comparisons test results showed there was significant.  Mean  difference  of  KP-KP1,  KP1-KP2,  and  KP2-KP3 were  respectively 10.75, 11.12, and 9.96. Conclusions: The nefroprotective effect and the optimal dose of ethanol extract of red beet can prevent renal cells damaging of mice induced by deep frying palm oil. Keywords: red beet, deep frying palm oil, histology of renal 
Efek Hepatoprotektif Ekstrak Etanol Bit Merah (Beta vulgaris L.) terhadap Kerusakan Sel Hepar Mencit (Mus musculus) yang Dipapar Minyak Kelapa Sawit Pemanasan Berulang Muthmainah, .; Widyaningsih, Vitri
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Red beet (Beta vulgaris L.) contains betalain pigments consisting of betacyanin and betaxanthine that can protect the liver from free radicals. This research aims to know the hepatoprotective effect of ethanol extract of red beet against liver histological damage of mice caused by reheated palm oil. Methods: This study was laboratory experimental research with the post test only control group design. This research used male Swiss Webster mice with 2-3 months old and ±20 g of body weight, each. Thirty samples of mice were divided into 5 groups, each group consisted of 6 mice. Sampling technique in this research was consecutive sampling. The normal control group (KKn) was given distilled water. The exposure group (KP) and the 1st, 2nd, 3rd treatment group (KP1, KP2, KP3), mice were given reheated palm oil. KP1, KP2, KP3 were not only given reheated palm oil, but also were given ethanol extract of red beet in dose 5.6 mg, 11.2 mg, and 22.4 mg. These treatments were done for 14 days. On the 15th day mice were killed with neck dislocation and livers were made for preparation with Paraffin Block method and were stained with Hematoxyllin Eosin (HE). Liver histological features were assessed based on quantifying of necrotic cells (pyknosis, karyorrhexis, karyolysis) of each 100 cells in the third zone. Data were analyzed using One-Way ANOVA test and Post Hoc test. Results: Data showed mean of necrotic cells in KKn, KP, KP1, KP2, KP3 respectively were 27.17±1.47, 75.33±1.61, 61.92±1.56, 51±1.54, 43.08±1.73 and mean difference between KP-KP1, KP1-KP2, KP2-KP3 were 13.42, 10.92, 7.92. The results of One-Way ANOVA test showed a significant difference of liver damage score in the five groups, p < 0.001. The results of Post Hoc test also showed significant difference among five groups, p < 0.001 and the most significant difference were between KP-KP2. Conclusion: Ethanol extract of red beet provides protective effect against mices liver histological damage caused by reheated palm oil and optimal dose of ethanol extract of red beet is 11.2 mg/20 g of body weight. Keywords: Ethanol extract of red beet, reheated palm oil, liver histological damage. 
Efek Granul Ekstrak Bawang Daun (Allium fistulosum L.) terhadap Mortalitas Larva Aedes aegypti L. Resitarani, Elisabeth Dea; Haryati, Sri; Wicaksono, Bagus
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Spring onion (Allium fistulosum L.) are known to contain saponin, tanin and flavonoid having larvicide effect. The purpose of this research is to know the effect of granules of spring onion extract (Allium fistulosum L.) on the Mortality of Aedes aegypti L. Larvae. Methods: This research was a laboratory experimental with the post test only controlled group design, which was done at “Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga”, Central Java. The subject used in this research was third stage of Aedes aegypti L. larvae. Subjects were taken with incidental sampling technique. The numbers of the subjects were 175 larvae, divided into 7 groups, so each group contained 25 larvae. This treatment was repeated 4 times.  For the control group it was used 100 ml of water. The other six groups used granules solution of spring onion extract, that were 1,400 mg, 1,700 mg, 2,000 mg, 2,300 mg, 2,600 mg, and 2,900 mg per 100 ml of water. The observation was held after 24 hours then the number of dead larvae was counted. The data were analyzed using One Way ANOVA followed by LSD Post Hoc. Lethal Concentration (LC) value was determined using Probit analysis. Results: There were significant difference of larvicide effect caused by granules of spring onion extract treatment (p = 0.000). The number of dead larvae increased along with the increase in the concentration of granules. LSD test indicated that there was significant difference between two groups except between groups 2,600 mg/100 ml and 2,900 mg/100 ml. The results of probit analysis showed that LC50 was 1,620.55 mg/100 ml and LC99 was 3,333.89 mg/100 ml. Conclusion: The granules of spring onion (Allium fistulosum L.) extract can cause death of Aedes aegypti L. larvae with LC50 in level of 1,620.55 mg/100 ml and LC99 in level of 3,333.89 mg/100 ml. Keywords: spring onion extract, granules, Aedes aegypti L. larvae 
Uji Banding Terjadinya Andropause antara Laki-Laki yang Lingkar Pinggangnya Besar dan Normal di Kecamatan Jebres Surakarta Kurniawan, Andreas Agung; Iryawan, Andri; Murti, Bhisma
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Andropause in men can be caused by large waist circumference. The population of Asian countries, including Indonesia, tends to undergo an increase of waist circumference. This study is aimed to compare the occurrence of andropause  between men with large and normal waist circumference. Methods: This study was a non-experimental analytic observational study with cross-sectional approach conducted in Jebres in September-October 2014. The subjects were 40 men selected using simple random sampling technique. The data obtained from physical measurements and questionnaire-led interviews were analyzed using multivariate logistic regression test to control confounding factors. Results: Twelve out of 15 men with large waist circumference (> 90 cm) experienced andropause and 16 out of 25 men with normal waist circumference (≤ 90 cm) did not experience andropause. On the other hand, 5 out of 10 men aged < 50 years experienced andropause and 23 out of 30 men aged ≥ 50 years did not experience andropause. Conclusion: Men with large waist circumference have risk experiencing andropause 3.06 times higher than men with normal waist circumference, although in this study the result is not significant (OR = 3.06; CI = 95% 0.59 to 15.95; p = 0.184 ). Keywords: Andropause, Waist Circumference 
Efek Nefroprotektif Ekstrak Daun Sirsak (Annona muricata L.) pada Mencit Model yang Diinduksi Parasetamol Hidayah, Nur; Suyatmi, .; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Soursop Leaf (Annona muricata L.) contains active compounds that have antioxidant effects consisting of flavonoid, alkaloid, tannin, triterpenoid and steroid. The aim of this research is to know the protective effect of Soursop Leaf Extract on the renal histological damage of  mice induced by paracetamol Methods: This study was experimental research with the post test only controlled group design. Samples were 30 male Swiss webster mice, 2-3 months old and ± 20 g of body weight. Samples were divided randomly into 5 groups, each group consisted of 6 mice. The normal group (KN) and negative control group (KKn) were given distilled water for 14 days. KP1, KP2, and KP3 were given soursop leaf extract in dose 5.6 mg, 11.2 mg, and 22.4 mg per g of body weight of mice, respectively, for 14 days. Paracetamol in dose 5.07 g per 20 g body weight of mice were given to KKn, KP1, KP2, and KP3 on the 12th ,13th ,14th days. On the 15th day mice were sacrificed and then renal organ were taken for histological preparation and stained with HE. Renal histological features were assessed based on quantifying of necrotic cells (pyknosis, karyorrhexis, karyolysis) of 50 epithelial cells from proximal tubule of right and left renal organ. Data were analyzed using One-Way ANOVA test and continued with Post Hoc (LSD) test. Results: The means of necrotic cells for KN, KKn, KP1, KP2, and KP3 were 8.17, 38.58, 26.92, 21.08 and 11.67, respectively. The One-Way ANOVA test showed a significant difference of renal damage score in five groups with p = 0.000. The Post Hoc LSD test also showed significant difference among five groups, p = 0.000. Conclusion: Soursop leaf extract can prevent the histological damage of renal cells on mice induced by paracetamol. Keywords: Soursop leaf extract, paracetamol, renal histological damage
Pengaruh Ekstrak Daun Lamtoro (Leucaena leucocephala) terhadap Waktu Kematian Cacing Ascaris suum, Goeze In Vitro Ramadhan, Mira Rizki; Dharmawan, Ruben; Mashuri, Yusuf Ari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background. Nematodes infection particularly, Ascaris lumbricoides  is cronic infection that make serious health problems. Leucaena leaf (Leucaena lecocephala) contain  leucanin, leucanon, mimosin and tannin that have been known to have athelmintic effect. This study aimed to determine the effect of Leucaena. Leucaena leaf  (Leucocaena leucocephala) extract towards death time of Ascaris suum, Goeze In Vitro.. Methods. This study was a laboratory experimental research using the post-test only controlled group design. The subject were adult worms that have length between 10 -20 cm. The sampling technique was used in purposive sampling. The subject was divides into 6 groups, each group consist of 4 worms, replication performed 4 times. Pirantel Pamoat 5mg/ml was used in Positive Control Group (KKP) and NaCl 0,9% was used in Negative Control Group (KKN). While the handling group was given by leucaena leaf extract in various concentration. 5% b/v was given by first handling group (KP1), 10% b/v was given for KP2, 20% b/v was given for KP3, and  30% b/v was given for KP4. Worms immersed every 60 minutes and counted the number of time. Data were analyzed with regression liner and probit analys. Results. The fastest of death time that given by leucaena leaf is KP4 in the amount of 375 minutest, followed by KP3, KP2 dan KP1. While the death time of  KKP aproximately  at 60 minutes dan 3360 minutes on KKN. The result of Pearson correlation test show close relationship between variation of the concentration and the time of death worms with a correlation coefficient 0,778 marked negative. The result of regression liner show significant p < 0.000,  r square .605 with regression equation is Y = 2704 – 503,464 X. The result of probit analys LC50 in the amount of  10,355%, LT50 is counted at 411,178 minutes LT99 is counted at 571,621 minutes.  While  37,055 minutes is counted for Pirantel Pamoat. Conclution. Based on this study, in can be concluded that the  Leucaena leucocephala leaf extract affect mortality of Ascaris suum in Vitro. The increasing of extract concentration in inversely propotional with the death time of worms. Keywords : Leucaena Leaf extract, death time,  anthelmintic 
Pengaruh Pemberian Jus Stroberi (Fragaria x ananassa) Terhadap Kerusakan Histologis Sel Hepar Mencit yang Diinduksi Rhodamin B Pratiwi, Risky; Muthmainah, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Strawberry contains flavonoid, anthocyanin, ellagic acid and vitamin C that can protect the liver from free radicals. The aims of this research are to know the effect of strawberry juice and the increasing of its dose on the liver histological damage of mice caused by rhodamine B. Methods: This study was laboratory experimental research with the post-test only control group design. The laboratory animal used in this experiment was male Swiss webster mice with 2-3 months old and ± 20 grams of each weight. Twenty eight samples were divided into 4 groups (KKn, KP, KP1 and KP2), each group consisted of 7 mice. The sampling technique in this research was consecutive sampling. The normal control group (KKn) was given distilled water, while the exposure group (KP), the 1st treatment group (KP1), and 2nd treatment group (KP2) were given rhodamine B. The mice of KP1 and KP2 were given strawberry juice in dose 0.4 ml/20 gWt and 0.8 ml/20 gWt. These treatments were done for 16 days, then on the 17th day mice were killed with neck dislocation and their livers were made for preparation with paraffin block method and stained with HE. Liver cell histological damages were assessed based on quantifying necrotic cells of each 100 cells in the first zone and third zone. The data were analyzed using One-Way ANOVA test and Post Hoc LSD test (α = 0.05). Results: The data showed mean of necrotic cells in KKn, KP, KP1 and KP2 were 38.64 ± 2.56; 157.64 ± 2.87; 90.07 ± 2.70; and 68.93 ± 2.56, repeatedly. The results of One-Way ANOVA test showed p = 0.000. The results of LSD test showed significant difference between KKn-KP, KKn-KP1, KKn-KP2, KP-KP1, KP-KP2, and KP1-KP2 with p = 0.000 for each groups. Conclusion: Strawberry juice reduces mice’s liver cell histological damage caused by rhodamine B and the increasing dose of strawberry juice can reduce liver cell histological damage. Keywords: Strawberry juice, rhodamine B, liver cell histological damage  
Pengaruh Pemberian Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Hepar Mencit (Mus musculus L.) yang Diinduksi Parasetamol Irawan, Alvian Oscar; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Kenikir leaf contains flavonoids, phenolic acid, saponin, carotenoids, vitamin A, vitamin C, Fe, Zn, and Cu. They are potential antioxidants being capable of protecting cells from free radical. The aim of this research is to evaluate the hepatoprotective effect of kenikir (Cosmos caudatus Kunth.) leaf’s ethanol extract on mice induced by paracetamol. Methods: This research was a laboratory-based experiment with the post-test only with control group design. The samples of 35 male Swiss webster mice, aged 2-3 months with ± 20 gram of weight were randomly divided into 5 groups. The groups were normal group (KN), negative control group (KK(-)), treatment group 1 (KP1), treatment group 2 (KP2), and treatment group 3 (KP3). Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg were administered daily for 14 days to KP1, KP2 and KP3, respectively. A toxic dosage of paracetamol was administered to KK(-), KP1, KP2, and KP3 on day 12th, 13th, and 14th. On day 15th, mice were sacrificed by neck dislocation and the liver were prepared and stained with HE for histology study. The degree of damage being due to the paracetamol was based on summation of pyknosis, karyorrhexis, karyolysis nucleus. Data were analyzed using One-Way ANOVA test (α = 0.05), followed by Post Hoc Multiple Comparisons (LSD) (α = 0.05) test. Results: The One-Way ANOVA test result was p = 0.000 (p < α). The statistical LSD test result was p = 0.000 (p < α), which showed significant differences in the hepatic damage between KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 group. Conclutions: The administration of kenikir leaf’s extract is able to prevent the damage in mice’s liver cells induced by paracetamol. Keyword: ethanol extract, kenikir (Cosmos caudatus Kunth.) leaf, paracetamol, liver cells, mice 
Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Ginjal Mencit (Mus musculus L.) yang Diinduksi Parasetamol Wibowo, Lina Kristanti; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Kenikir leaves contain various kind of antioxidants, such as vitamin C, β-carotene, minerals, and polyphenols which are known to protect kidneys from oxidative stress caused by free radical exposure. The purpose of this study is to evaluate the nephroprotective effect of Kenikir leaf extract toward histological damage of the mices kidney cells induced by Parasetamol. Methods: This was a laboratory experimental study with the posttest only controlled group design. The samples were 35-Swiss webster male mice, aged 2-3 months, and weight about 20 gram. Samples were divided into five groups randomly. Normal group (KN) was given distilled water and Negative control (KK(-)) was given Parasetamol. Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg of kenikir leaves were given daily for 14 days to treatment group 1 (KP1), treatmen group 2 (KP2), and treatment group 3 (KP3), respectively. A toxic dosage of Parasetamol as much as 5.07 mg, was given to negative control group and treatment groups on day 12th, 13th, and 14th. On day 15th , mice were sacrificed by neck dislocation. The kidneys were taken for histological preparation and stained with HE. The histological damage was assessed based on quantifying the pyknosis, karyorrhexis, and karyolysis on 50 proximal tubuli of epithelial cells. The data were analyzed using One-way ANOVA test (α = 0.05) and Post Hoc Multiple Comparisons test (α = 0.05). Results: The most damaged cells were found in KK(-) and the least in KN. The significant difference was observed among groups (One-way ANOVA test p = 0.000). The significant difference was observed between groups (Post Hoc test p = 0.000). They were KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusions: Ethanol extract of Kenikir leaves can prevent the histological damage of the mices kidney cell induced by Parasetamol. The increasing dose of the extract is followed by the increase of protective effect. Keywords: Kenikir leaves, histological damage, mices kidney, Parasetamol 

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