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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol 39, No 4 (2014): December" : 10 Documents clear
EFFECT OF FSH β-SUB UNIT AND FSHR GENES POLYMORPHISMS ON SUPEROVULATORY RESPONSE TRAITS Andreas, E.; Arifiantini, I.; Saputra, F.; Ishak, A. B. L.; Imron, M.; Sumantri, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.197-203

Abstract

Follicle stimulating hormone (FSH) is a pituitary expressed glycoprotein hormone that regulatesreproduction in mammals which composed of α and β-sub unit. The β-sub unit dictates its bindingspecificity with their receptor (FSHR). This study aimed to identify polymorphism of FSH β-sub unitand FSHR genes, and its effect to superovulatory response traits on superovulated cows. Study was doneon 32 cows including Angus, Friesian Holstein (FH), Limousin, Simmental and Brahman in CipelangLivestock Embryo Center. Cows used have been treated superovulation and mated by artificialinsemination. Superovulation response (SR), ovulation rate (OR), fertilization percentage (FP) andviable transfer embryo percentage (VP) were analyzed to investigate the effect of FSH β-sub unit andFSHR polymorphism. Allele frequency of FSH β-sub unit|PstI and FSH|AluI were opposite withinspecies. Mostly B allele and C allele for FSH β-sub unit and FSHR respectively have a high number inBos taurus species while those were in contrast in Bos indicus species. The highest heterozygosity wasfound in FH cattle (0.250) for FSH β-sub unit and Brahman (0.333) for FSHR. Significant effect was found between FSHR gene polymorphism with ovulation rate where CC genotype was higher (P<0.05)than CG and GG genotypes.
PRE-SLAUGHTER STRESS ESTIMATION BY FOURIER TRANSFORM INFRARED SPECTROSCOPY ANALYSIS Widiyanto, S.; Widiyono, I.; Purwono, P.; Astuti, P.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.242-248

Abstract

The objective of the study was to analyze the potential of fourier transform infrared (FTIR) asstress estimation instrument through measurement of catecholamine (CA) and cortisol (CO) levels byinvasive and non-invasive methods. Twelve heads of Ongole grade (PO) cow from slaughterhouse wereused in this study. Twenty four urine samples of pre-slaughter and slaughtering were collected toevaluate the CA and CO levels by ELISA method and Fourier Transform Infrared (FTIR). Data of CAand CO levels were statistically analyzed to determine the difference between pre slaughter andslaughter conditions. FTIR spectra were analyzed using chemo-metrics software. The results showedthat the levels of urinary cortisol were 2.12±1.68 ng/dl of pre-slaughter and 7.58±3.89 ng/dl ofslaughtered and the levels of urinary catecholamine in pre slaughter and slaughter were 3.07±2.05 and4.15±2.68 ng/dl respectively. The results showed a correlation between the FTIR spectra and the resultsof ELISA analysis. FTIR spectrums were distributed in different quadrants, this was caused bydifferences of CA and CO levels between pre-slaughter and slaughtered. It can be concluded that FTIRcan be supposed to analyze the status of stress in animals, especially in cattle.
GENETIC DIVERSITY OF KEJOBONG GOAT BASED ON MITOCHONDRIAL DNA D-LOOP SEQUENCE M. F. Harlistyo; S. Sutopo; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.204-209

Abstract

This study was aimed to find out the diversity of mtDNA D-loop at Kejobong goat. The completemtDNA D-loop sequence of 12 goat blood samples were analyzed from 4 different location inPurbalingga Regency, Central Java province, sub-districts Kejobong, Pangadegan, Bukateja, andKaligondang. The mtDNA D-loop was extracted from blood sample. DNA obtained were amplified byPCR (Polymerase Chain Reaction) method using primers (5’-tcactatcagcacccaaagc-3’) as forward and(5’-ggcattttcagtgccttgct-3’) as reverse and subsequently sequenced. After nucleotide sequencing analysisconducted, 548 bp along was obtained. Nucleotides were then aligned with Capra hircus (GenBankAccess No.: KF952601.1) and apparently there were 11 different sites on the segment of mtDNA Dloop.Five sites could be used as a specific marker to distinguish between the Capra hircus andKejobong goat, namely at the site of 317 (A-G), 403 (T-C), 434 (T-C), 537 (C-T), and 553 (A-G).Nucleotide sequence analysis also contained seven different haplotypes. It was concluded that thedistribution of the different sites showed different haplotype patterns in Kejobong goat.
Front-matter (December 2014) Kurnianto, Edy
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.%p

Abstract

CARCASS QUALITY, MARBLING AND CHOLESTEROL CONTENT OF MALE BALI CATTLE FED FERMENTED COCOA SHELL Suryanto, E.; Bulkaini, B.; Ashari, A.; Karda, I . W.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.249-255

Abstract

The experiment was conducted to evaluate the effect of fermented cacao shell on the carcassquality, percentage of non carcass components, marbling and cholesterol content of Bali cattle. NineBali cattle of 1.5-2 year old and 165-175 kg weight were grouped into three feeding trials. The firstgroup P0 was fed ration containing 30% cacao shell fermented with its own microorganism and 70%corn straw, the second group P1 was fed ration containing 30% cacao shell fermented with ruminal fluidand 70% corn straw, and the third group P2 was fed ration containing 30% cacao shell fermented withBioplus and 70% corn straw. All cattle were also given commercial concentrate amounting to 1% oftheir bodyweight. The cattle were slaughtered at the end of feeding trial and their carcasses, meat andnon carcass components were analysed. Experimental design used was completely randomized design.The results showed that carcass percentage, back fat thickness, rib eye area and meat index of Bali cattle were as follows group PO 53.33%, 3.08 mm, 59.65 cm2, 0.79%; group P1 52.64%, 5.31 mm, 58.52 cm,0.82% and group P2 5.32%, 5.7 mm, 57.75 cm, 0.79%, respectively. The marbling and cholesterolcontent of Bali beef were group PO 2.65% and 71.25 mg/100g, group P1 2.12% and 48.75 mg/100g,and P2 2.63% and 74.50 mg/100g. It could be concluded that fermented cacao shell could be used asfeed ingredient without any effect on the carcass and meat quality, marbling and cholesterol contents ofmale Bali beef.
Back-Matter (December 2014) Kurnianto, Edy
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.%p

Abstract

THE EFFECT OF CONCENTRATE CONTAINING PROBIOTICS ON FERMENTATION CHARACTERISTICS, METHANOGENESIS AND In vitro NUTRIENT DIGESTILITY Santoso, B.; Hariadi, B. Tj.; Abubakar, H.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.210-216

Abstract

The aim of the experiment was to evaluate the effect of probiotic addition in concentrate onfermentation characteristics, methane (CH4) production and in vitro nutrient digestibility. Two strainslactic acid bacteria (LAB) i.e. Lactobacillus plantarum and Lactobacillus acidhophilus, and one strainyeast of Saccharomyces cerevisiae was used as probiotic.This experiment was arranged in a completelyrandomized design consisted of 4 treatments as follows grass silage (G); grass silage + concentratewithout probiotic (G+A); grass silage + concentrate containing L. plantarum and. S. cerevisiae (G+B);grass silage + concentrate containing L. acidophilus and. S. cerevisiae (G+C); grass silage + concentratecontaining L. plantarum and L. acidophilus (G+D). Data were analyzed as completely randomizeddesign and followed by Duncan’s multiple range test. The results showed that the concentrate containingLAB varied 1.5 × 106 and 3 × 107 cfu/g, and 3 × 103 cfu/g of S. cerevisiae. Combination between L.plantarum and S. cerevisiae (G+B), and L. acidophilus and S. cerevisiae (G+C) in concentrate increased(P<0.01) propionic acid proportion. The average CH4 production in concentrate containing probiotic(G+C, G+D and D+E) was lower by 6.9% (P<0.01) compared to concentrate without probiotic (G+B). The in vitro dry matter (DM) and neutral detergent fiber (NDF) digestibility were higher (P<0.01) by25.7% and 6.3% respectively, in grass silage substrate with concentrate containing probiotic (G+C, G+Dand G+E) than in grass silage with concentrate without probiotic (G+B). In conclusion, addition ofprobiotic in concentrate could increase in vitro propionic acid proportion, DM and NDF digestibility andreduce CH4 production.
FEEDING EFFECT OF INULIN DERIVED FROM Dahlia variabilis TUBER ON INTESTINAL MICROBES IN STARTER PERIOD OF CROSSBRED NATIVE CHICKENS Krismiyanto, L.; Suthama, N.; Wahyuni, H. I.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.217-223

Abstract

The purpose of the research was to study the effects of feeding inulin derived from Dahliavariabilis tuber powder and extract on the existence of intestinal microbes in crossbred native chickenstarter. Experimental animals were 280 unsex crossbred native chickens and powder and extract ofdahlia tuber as source of inulin, which were started to be fed on day 22. The present experiment wasassigned in a completely randomized design with 7 treatments and 4 replications (10 birds each). Thetreatments were: T0 (basal diet/BD), T1 (BD+0.4% powder form), T2 (BD+0.8% powder form), T3(BD+1.2% powder form), T4 (BD+0.39% extract form), T5 (BD+0.78% extract form), and T6(BD+1.17% extract form). Parameters measured were the number of Lactic acid bacteria (LAB),Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage anddaily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan andpolynomial orthogonal test. The results indicated that feeding inulin derived from dahlia tuber powderand extract form significantly (P<0.05) increased LAB population and DBWG, but decreasedEscherichia coli number and intestinal pH, and slow the rate of passage. In conclusion, feeding inulin ofdahlia tuber powder was optimum at 0.9% but that of extract is linier until 1.17%.
ISOLATION AND LIGNOCELLULOLYTIC ACTIVITIES OF FIBER-DIGESTING BACTERIA FROM DIGESTIVE TRACT OF TERMITE (Cryptothermes sp.) Tampoebolon, B.I.M.; Bachruddin, Z.; Yusiati, L.M.; Margino, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.224-234

Abstract

The objectives of this study were to obtain the fiber-digesting bacteria isolates from termitedigestive tract and to determine the optimum conditions of growth and production of cellulase, xylanaseand ligninase enzyme of isolate. The first study was conducted to isolate and select the fiber-digestingbacteria from the digestive tract of termites based on the highest activity of cellulolytic (S), xylanolytic(X) and lignolytic (L). The second study was optimation of the growth conditions of bacteria and theenzyme production due to effect of rice straw substrate and nitrogen. The material used were dry woodtermites, rice straw, and culture medium. The design used was a completely randomized factorial design,in which the first factor was rice straw substrate (1, 2, and 3% W/V), while the second factor wasnitrogen (0.1, 0.2 and 0.3% W/V). Variables measured were cellulase, xylanase and ligninase activities.Results of the first sudy showed that the isolates obtained consisted of 3 types, those were cellulolyticbacteria (S1, S2, and S3), 3 types of bacteria xylanolytic (X1, X2, and X3) and 3 types of bacteria lignolytic(L1, L2, and L3). Meanwhile, results of the second study showed that isolates of S2, X3, and L1 had thehighest activity, those were 1.894 U/mL, 1.722 U/mL and 0.314 U/mL, respectively. In conclusion, the addition of 1% level of rice straw substrate and 0.3% of nitrogen showed the highest enzyme activity oncellulase, xylanase and ligninase.
SOME FACTORS AFFECTING INTENSIVE REARING ADOPTION ON BEEF CATTLE FARMERS IN WAJO REGENCY, SOUTH SULAWESI PROVINCE Baba, S.; Dagong, M.I.A.; Risal, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.235-241

Abstract

Land use conflict among agriculture and animal husbandry caused beef cattle must be keptintensively. The objective of this research was to identify adoption level of beef cattle intensive rearingand some factors that affecting farmers adoption. Research design was survey with 90 respondents froma total of 578 farmers. Data were obtained through interview and observations using questionnare withclose question. The data were analyzed with multiple regression models. The level of intensive rearingadoption (dependent variable) was measured based on housing system, feeding, reproduction, healthmanagement and feces utilization. While, independent variables were based on the intensity ofextension, relative adventage, subjective norm, control of behavior, attitude, age, land area and scale ofbusiness. Level of housing system, feeding, reproduction and health management were in the medium level (average >50% of farmers), while feces utilization was in the lowest level. Factors that positivelyaffected farmer adoption in Wajo regency were the ability of the farmers to control their behavior(p<0.01), farmer position in social community or subjective norm and relative adventage weresignificant with p<0.05. Another case with contact to the extension, age, attitude, land area and scale ofbusiness were no significant affected farmers adoption. To enforce adoption of intensive rearingtechnology, extension program should be developed to influence farmers psychology such profit level ofintensive rearing and convenience on cattle handling.

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