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Majalah Obat Tradisional

TradMedJ
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Published by Universitas Gadjah Mada

ISSN : 14105918 EISSN : 24069086 DOI : -

Core Subject : Health,

Health Professions Medicine & Pharmacology Public Health

raditional Medicine Journal (Majalah Obat Tradisional), or Trad. Med. J. (ISSN 1410-5918 (print) and ISSN 2406-9086 (online)), is an international scientific journal published by Faculty of Pharmacy, Universitas Gadjah Mada, three times annually. Collaborating with Indonesian Pharmacist Association, Daerah Istimewa Yogyakarta, and we dedicate our journal to researches and development in traditional medicine. The journal receives papers on research laboratory, field research, and case studies of traditional medicine and its constituent, covering research topics including raw materials, cultivation, phytochemical, pharmacological effects and toxicology, formulation, and biotechnology.

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Articles 10 Documents

Search results for , issue "Vol 22, No 2 (2017)" : 10 Documents clear

Synergistic Effects of Doxorubicin and Cardenolid Glycosides of Calotropis Gigantea Root on Cervical Cancer Hela Cell Line Roihatul Mutiah; Risma Aprinda Kristanti; Siti Maimunah
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Cardenolid glycosides (CGs) has been known has high anticancer activity against several types of cancers, for example, breast cancer, lung cancer, prostate cancer, melanoma, neuroblastoma, myeloma and leukemia in the in vitro and in vivo research. The aim of our study was to know the synergistic potency of CGs improving the efficacy of doxorubicin in cervical cancer. Activities from combination of doxorubicin and CGs was measured by MTT colorimetric methode. Combination Index was used as combination efficacy parameter. The results showed that the IC50 of CGs was 1,023 μg/mL. CGs demonstrated selective activity in inhibiting the growth of HeLa cells by selectivity index > 3 (241.9). Combination test for CGs and Doxorubicin showed strong synergistic effect in doses 200 ug/mL Doxorubicin and 1.79 ug/mL CGs. The synergistic effect has been shown by 10 combination doses for the doses below the IC50. CGs increases the efficacy of Doxorubicin for the doses below the IC50, thus CGs can be recommended as co chemotherapy in cervical cancer treatment.

Capsule Formulation of Ethanolic Extract of Pasak Bumi (Eurycoma Longifolia Jack.,) and its Effect on Human Health Vital Signs Laela Hayu Nurani; Eka Kumalasari; Abdul Rohman; Sitarina Widyarini
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Pasak bumi (Eurycoma longifolia) has the potential to be developed as antihypertensive, antipyretic, aphrodisiacs and health supplements. The use of E. longifolia as a traditional medicine needs to be pursued in the form of more effective and appropriate formulation. The capsule preparations are easy to make and cancover the bitter taste of E. longifolia. Clinical trials in this study use design pre-post treatment in healthy humans. Subjects used were male - healthy men and healthy women who met inclusion criteria and were subjected with formulated capsule for 14 days. The study resulted capsule formula comprising of the ethanolic extract of E. longifolia 300 mg, vivapur 101 300 mg, 58 mg maydis starch, aerosil 3%, talc 2%, and Mg stearate 1%. The results showed that the capsule of E. longifolia did not affect the value of heart rate, respiration rate, body temperature and weight (P > 0.05), based on paired t-test, but they causes a decrease in blood pressure of healthy human. The ethanol extract of E. longifolia caused vasodilation of blood vessels that can be used in antihypertensive therapy.

Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method Retno Wahyuningrum; Ritmaleni Ritmaleni; Tatang Irianti; Subagus Wahyuono; Takushi Kaneko; Titik Nuryastuti
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Tuberculosis (TB), in which caused by pathogenic bacteria, Mycobacterium tuberculosis, has become the major causes of death among all of infectious diseases. The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) has created a need to discover a new antituberculosis drug candidate. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv using the Microplate Alamar Blue Assay (MABA) method. T. crispa extract was prepared by maceration in ethanol (96%) and antituberculosis activity was carried out using MABA method. The result of this study showed that ethanolic extract of T. crispa exhibit antituberculosis activity with minimum inhibition concentration of 12.5 mg/ml.

The Effectiveness Test of Oil Phase Ointment Containing Snakehead Fish (Channa striata) Extract on Open Stage II Acute Wounded Wistar Strain Male Rats Fransisca Daisa; Mohamad Andrie; Wintari Taurina
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

The snakehead (Channa striata) contained fatty acids omega-3 and omega-6 are useful as nutrients in accelerating the wound healing process. This study aims to determine the effectiveness of the oil phase extract ointment snakehead in the healing of acute wounds open stage II. Testing the effectiveness of wound healing using 6 groups: normal, gel bioplacenton (positive control), ointment base (negative control), ointments snakehead extract oil phase concentration of 10; 20 and 40% of the 4 rats with acute wounds open stage II and observation until day 16 injury. The area of the wound was measured with the image J program Macbiophotonic, calculated% healing power, and AUC values. A statistical test to the total AUC values per rat with SPSS for Windows 22.0 program using One Way ANOVA and Post-hoc LSD test. The results showed a significant difference in the negative group with ointment concentration of 20% (p <0.05). The treatment group phase ointment fish oil extract is effective for wound healing is best to have a concentration of 20% for percentage of wound healing power of 97.157% and the average AUC value of 865.683% of the day.

Effect of Papaya Seed Extract (Carica papaya Linn.) on Glucose Transporter 4 (GLUT 4) Expression of Skeletal Muscle Tissue in Diabetic Mice Induced by High Fructose Diet Devyani Diah Wulansari; Achmad Basori; Suhartati Suhartati
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Ethnobotany surveys show that papaya seeds are widely used as herbs for the management of some diseases such as abdominal discomfort, pain, malaria, diabetes, obesity, and infection. This research was conducted to analyze the effect of papaya seed extract on GLUT4 expression on skeletal muscle tissue of DM type II model induced by high fructose diet. This study used 24 animals, divided into 4 groups of negative control group, treated with papaya seed extract 100 mg / kgBB, 200 mg / kgBW and 300 mg / kgBW, was adapted for 14 days then induced by fructose solution 20% Orally with a dose of 1.86 grams / kgBB for 56 days. The treatment group was given papaya seed extract in accordance with the dose of each group for 14 days. GDP levels was measured using a spectrophotometer. Skeletal muscle tissue is used on the gastrocnemius part. GLUT4 expression was measured through a Immunoreactive Score (IRS) method with immunohistochemical staining using GLUT4 polyclonal antibodies. Comparative test results showed that there were significant differences between groups (p <0.05) in final GDP variables and GLUT4 expression. Pearson correlation test results show that the value p = 0.001, meaning there is a significant relationship between GLUT4 expression with final GDP levels. The result of simple linear regression analysis showed that p = 0,000 (<0,05), meaning that dose of papaya seed extract had a significant influence on GLUT4 expression.

Anti-inflammatory Activity of Camellia sinensis, l. Extract Cream Combined with Vitamin C as Antioxidant on Croton Oil-induced Inflamation in Male Mice Strain BALB/C Nining Sugihartini; Ratih Saridewi; Ulfa Ramdhani M; Fitri Rahmawanti; Sapto Yuliani; Vivi Sophia
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Green tea extract cream contains epigallocatechin gallate (EGCG) as the active ingredient for anti-inflammatory. Epigallocatechin gallate is easyly oxidized and able to reduce its effectivity as an anti-inflammatory. Therefore, an addition of antioxidants in order to increase its stability is required. The purpose of this study was to determine the effect of adding the antioxidant Vitamin C on the effectivity of green tea extract as an anti-inflammatory. This study uses 6 groups of male mice strain BALB/C which were given treatment as follows: normal control, negative control, base cream, green tea extract (0.2%), Vitamin C cream (1%) and green tea extract cream with addition of Vitamin C. The anti-inflammatory activity was evaluated based on the expression of COX-2, inflammatory cells and the thickness of the epidermis in the skin tissue of mice after given crotton oil (0.1%) on the back for the induction of inflammation. After treatment cream for 3 days, mice were sacrificed for histopathological tissue preparations made with hematoxylin eosin staining and immunohistochemistry COX-2. Data were analyzed statistically with one way Anova followed by t-test to determine differences between groups at a significance level of 0.05. The test results indicate that cream of green tea extract is higher in decreasing inflammatory parameters in comparison with cream of Vitamin C, except in the thickness of epidermal parameter. Green tea extract cream with the addition of Vitamin C is higher in reducing inflammatory parameters than cream of green tea extract or cream of Vitamin C. The decline percentage of cells that express COX-2, inflammatory cells and the thickness of the epidermis in the each of groups were cream of green tea extract:57.95%;53.75%;34.83%, cream of Vitamin C:48.76%;34.96%;34.27%, cream of green tea extract and Vitamin C:61,89%;65,54%;46.30%, respectively. Based on the results of this study, it can be concluded that anti-inflammatory activity of green tea extract cream increased due to the addition of 1% vitamin C as an antioxidant.

Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process Kintoko Kintoko; Hanifah Karimatulhajj; Trie Yuni Elfasyari; Ersi Arviana Ihsan; Teguh Adiyas Putra; Puspawan Hariadi; Citra Ariani; Nurkhasanah Nurkhasanah
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was overed by amputation as the result of improper handling. Herbal treatments could be an alternative treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol (FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction. Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control group (non diabetes), negative control (diabetes + topical application of biocream®), positive control (diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%) in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB 10% has significant differences potention in wound healing process in diabetic rats macroscopically that accelerates wound contraction compared with negative and positive control groups (p<0,05).

In Vitro α-Glucosidase Inhibitory Activity of Ethanol Extract of Buas-buas (Premna serratifolia Linn) Dini Hadiarti
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

In 2008, diabetics in Indonesia has reached 8,5 million of 11,1 % prevalence in West Kalimantan. It was estimated to reach 14,1 million in 2035. The treatment of diabetes may occur adverse reactions such as hypoglycemia, lipoatrophy, lipohypertrophy, lactic acidosis, gastrointestinal disturbances, allergic reactions, and obesity. Therefore, it is necessary to find an alternative medicine to overcome this problem. Buas-buas (Premna serratifolia Linn) could supposedly be an anti diabetic by inhibiting the α-glucosidase enzyme, due to the compositions of secondary metabolites, cardioprotective activity and it has a similar genus with Premna serratifolia Linn. The soxhlet extraction result from Premna serrtifolia Linn leaf powders produced of 34,1 % yield. Meanwhile, the activity of α-glucosidase in vitroinhibition test with amicroplate reader in various concentrations of samples of 0,125, 0,5, 1, 1,5, 2, dan 2,5 2% (b/v) were obtained the absorptions of 37,95, 74,77, 86,15, 91,03 and 91,69 %, respectively. The extraction of Premna serratifolia Linn leaf revealed that the concentration of 2 % of sample inhibited in vitro α-glucosidase with a percentage of 91,03 %. The extraction of Premna serratifolia Linn leafinhibited α-glucosidase of 97 % from the percentage of Acarboseinhibition.

The Profile of Anti-inflammatory Activity of Syzigium Aromaticum Volatile Oil in Lotion with Variation Composition of Oleic Acid and Propylene Glycol as Enhancer Fitriah Ardiawijianti Iriani; Nining Sugihartini; Tedjo Yuwono
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Essential oil of clove (Syzygium aromaticum) containing eugenol has an anti-inflammatory activity. The study was aimed to develop the formulation of lotion by adding of oleic acid and propylene glycol as penetration enhancer. The effect of enhancer composition was also studied. Lotion was prepared with the composition of oleic acid (AO) and propylene glycol (PG) as follow: 1:0 (FI), 0,5:0,5 (FII), 0:1 (FIII). Capacity an anti-inflammatory of formulation based on parameters of the amount of cells with COX-2 expression, the number of inflammatory cells and the epidermis thickness was evaluated using male mouse strain BALb/C induced by crotton oil as inflammatory agents. The results showed that the increasing composition propylene glycol caused the decreasing of the amount of cells with COX-2 expression (p <0.05), the inflammatory cells (P <0.05) and the epidermis thickness (p <0.05)

Herbal Combination Effect on the Quality of Semen and Libido in Ongole Cattle Cross-breed Lukman Affandhy; Dian Ratnawati; Mochamad Luthfi
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Bulls fertility has an important role to conception, so bulls should have a high libido, high quality semen, and good performance. This study aimed to improve the quality of semen bulls using traditional herbal medicine by utilizing a combination of medicinal plants. The study used 20 heads of Peranakan Ongole (PO) bulls, is divided into four trials traditional herbs, namely Curcuma xhanthorriza, Curcuma eoriginosa, Alpinia galanga, Andrographis paniculata, Piper retrofractum, Eurycom longifolia, Pimpenella purwatjan, each of which is F1 (15%, 15%, 20%, 5%, 30%, 15% and 0%); F2 (15%, 15%, 20%, 5%, 30%, 10% and 5%); F3 (15%, 15%, 20%, 5%, 25%, 20% and 0%); and F4 (15%, 15%, 20%, 5%, 25%, 15% and 0%). Giving the bull 50 g dose of herbal formula every 300 kg of body weight bull mixed with 200 ml honey and 5 eggs. Bulls were given traditional herbal formula once a week orally. The dependent variable of sperm volume, quality of sperm and strength libido were assessed with randomized block design one way. The results showed that all four traditional herbal formulas that are given to PO bulls did not appear to significantly affect the volume of semen and libido for a month with an average volume of 3.8 ± 2.8 cc / ejaculate and libido 159.9 ± 20.2 seconds. Sperm motility, sperm concentration, percentage of live sperm, and sperm abnormalities in PO bulls were given traditional herbal medicine with four different formulas also not significant (P> 0.05); with as well as the color, pH and consistency is still within the normal range. It was concluded that the four herbal formula derived from a mixture of traditional medicine can be given to bulls in order to increase libido and semen quality. It is suggested for granting herbal medicine ingredient formula of traditional medicine that are cheap and easily obtained to bulls.

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Pudyastuti Kusumaningrum

Contact Email
mot.farmasi@ugm.ac.idm

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mot.farmasi@ugm.ac.id

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Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA