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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 14 Documents
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Search results for , issue "Vol 7, No 2 (2017)" : 14 Documents clear
Cytotoxic of Ganoderma lucidum in Colon Cancer through Cyclooxygenase 2 (COX-2) as Its Molecular Target Setiawati, Agustina
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.14

Abstract

Many studies were designed explore chemopreventive activity of natural products on colon cancer especially addressing COX-2 as molecular target. Another promising source of natural product that potentially exhibit anticancer activity on colon cancer is Ganoderma lucidum. This study assessed selectivity of cytotoxic effect of G. lucidum extract on WiDr to Vero cells and investigated molecular mechanism on COX-2. G. lucidum ex-tract was prepared by reflux extraction method; in vitro anticancer was assayed by MTT method on WiDr and Vero cell line. This study applied apoptosis induction assay to observe cell death mechanism using double staining method; further COX-2 expression was stained by immunocytochemistry method. G. lucidum extract has cytotoxic effect on WiDr cells with IC50 135 µg/mL. However, the cytotoxic effect had low selectivity to-wards Vero cells with Selectivity Index (SI) 3.66. The extract induced apoptosis and suppressed COX-2 ex-pression in WiDr cells. G. lucidum extract was potential to be developed as anticancer agent towards colon cancer.
Prevention of Dengue Hemorrhagic Fever (DHF) Associated with the Aedes aegypti Larvae Presence based on the Type of Water Source Hidayah, Nurul; Iskandar, Iskandar; Abidin, Zainal
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.05

Abstract

Aedes aegypti is the vector of Dengue Hemorrhagic Fever (DHF) disease. Containers are breeding places for DHF vector and the most commonly found larvae in the bath water containers. The presence of Aedes aegypti larvae in a container could be affected by the type of water source besides the container’s color, material, location, lid existence and the container’s drain frequency. This study was conducted to determine the associate of water source type with larvae presence and the additional factors. This study used observational analytic with case control design. The case group consisted of households using well water and the control group consisted of households using tap water with a sample size of 130 households for each group. The sample was collected by proportional random sampling in five villages. The data was analyzed using a regression logistic test. The significant variables associated with the presence of larvae were the water source type (OR=1.923), container’s color (OR=2.345), container’s location (OR=2.241), container’s lid existence (OR=2.122) and the container’s drain frequency (OR=2.260). This study did not consider the significant association of the container’s material. The dominant variable associated with the presence of larvae was the container’s drain frequency which was controlled by the water source type, container’s color and container’s location.
Cysteine, Malondyaldehide (MDA) and Glutathione (GSH) Levels in Marasmic Type Malnutrition Cahyani, Dwi Indah; Puryatni, Anik; Permatasari, Nur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.11

Abstract

Micronutrien deficiency in severe malnutrition will reduce antioxidant capacity that needed for oxidative stress defense. Cysteine, a non-essential amino acid, is one of an important component for reduced glutha-tione (GSH). This study aims to prove the difference between the levels of cysteine, MDA and GSH levels in children with marasmic malnutrition and well-nourished children and prove whether there is a relationship between those parameters. Fiftysix patients participated in this study were grouped into two groups of sam-ples that were marasmic type malnutrition group (28 patients) and control groups that were well nourished group (28 patients). Examination begins with a complete laboratory screening, followed by examination of cysteine, MDA and GSH level. Of the 28 patients included in marasmic type malnutrition group consisting of 15 male patients (53.6%) and 13 female patients (46.4%), while the well-nourished group consisted of 13 male patients (46.4%) and 15 female patients (53.6%). The average age is 54.61±56.35 months in the group of ma-rasmic type malnutrition and 48.25±45.34 months in the well-nourished group. By using the Mann Whitney test, there were significant difference between the levels of cysteine and GSH in marasmic malnutrition and control group (p = 0.000 and p = 0.000 respectively). Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels in marasmic malnutrition children shows no significant correlation (R = -0.206, p = 0.294; R = -0.036, p = 0.856; R = 0.210, p = 0.284 respectively). In well-nourished group Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels also shows no significant correlation (R = -0.053, p=0.789; R = -0.146, p = 0.458; R = -0.079, p = 0.688 re-spectively). From this study it can be concluded that there are significant differences of the levels of cysteine and GSH between severe malnutrition groups compared with the control one. But this study shows no signif-icant correlation between the levels of cysteine and GSH levels in marasmic type malnutrition and well-nourished children.
Microhabitat Assessment of Mangrove Pit Viper Cryptelytrops purpureomaculatus in Restricted Mangrove Area, Dumai, Riau Province, Sumatera Kurniawan, Nia; Priambodo, Bagus; Nugraha, Fitra Arya Dwi; Maulidi, Andri; Kurnianto, Agung Sih
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.01

Abstract

Bandar Bakau is a mangrove conservation area with approximately 20 ha that preserves many kinds of fauna, including Mangrove Pit Viper Cryptelytrops purpureomaculatus. However, this species and its habitat are still poorly studied in Indonesia, particularly Sumatera island, despite it has a wide distribution in Southeast Asian countries. The main purpose of this study is to evaluate the microhabitat of Mangrove Pit Viper C. purpureomaculatus that found at a restricted mangrove area in Dumai, Riau Province. Visual Encounter Survey (VES) and deep interview methods were used in this study. The survey found six individuals of C. purpureomaculatus that mostly basking on the Avicennia marina and artificial building. Although Bandar Bakau is a tourism object, but no snakebite cases were found.
Growth and Development of Apogamous Adiantum lunulatum Burm. f. Gametophyte from Dry and Humid Areas in Java Island Novitasari, Risti; Chikmawati, Tatik; Sulistijorini, Sulistijorini
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.15

Abstract

Adiantum lunulatum Burm. f. has been widely used in aesthetic needs and medical fields. Detail information about the life cycle of A. lunulatum is still unknown, particularly about its gametophyte generation. Present work aimed to study and compare the growth and development of the gametophytes of A. lunulatum from dry and humid areas. Research method consists of two stages: (1) Spores were collected from two locations, Pasuruan and Bogor and (2) Spores were planted and observed the growth and development of gametophyte. The result showed that natural habitat of the plant in various dry and humid areas, affecting the rate of growth and development rates of A. lunulatum gametophyte. The gametophyte from the dry area showed faster rate of growth and development than that of humid areas. The spores collected from dry area need 9 weeks for germination, growth, and development while the spores of humid area took 22 weeks.
In Silico Screening and Designing Synthesis of Cinchona Alkaloids Derivatives as Potential Anticancer Hanafi, Muhammad; Rosmalena, Rosmalena; Prasasty, Vivitri Dewi; Udin, Linar Zalinar; Primahana, Gian
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.06

Abstract

P-glycoprotein (P-gp) resistance in cancer cells decreases intracellular accumulation of various anticancer drugs. This multidrug resistance (MDR) protein can be modulated by a number of non-cytotoxic drugs. We have screened 30 chincona alkaloids derivatives as a potent P-gp inhibitor agent in silico. Hereby, we report the highest potential inhibitions of P-gp is Cinchonidine isobutanoate through molecular docking approach. with affinity energy -8.6 kcal/mol and inhibition constant, Ki is 4.89 x 10-7 M. Cinchonidine isobutanoate is also known has molecular weight below 500, Log P value 3.5, which is indicated violation free of Lipinski`s rule of five. Thus, Cinchonidine isobutanoate is the most potent compound as anticancer compare to other Cinchona alkaloids. Ultimately, we design Cinchonidine isobutanoate for further lead synthesis by using DBSA, act as a combined Brønsted acid-surfactant-catalyst (BASC) to obtain high concentration of organic product by forming micellar aggregates which is very powerful catalytic application in water environment.
Study of Serum Lactate Dehydrogenase and Gamma-Glutamyl Transpeptidase in Breast Cancer Patients receiving Chemotherapy. Basnyat, Anil Singh; Jha, Abimanyu; Pathak, R; Shrestha, Bhupal Govinda
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.07

Abstract

Breast cancer (BC) is the most common type of cancer worldwide, being a major cause of morbidity in female. In Nepal it is the second most common type of cancer among females of perimenopausal age group. More than one quarter of the BC diagnosed in young Nepalese female, with familial history of breast cancer, early pregnancy, longer lactation and estrogen exposure and often with tumors showing aggressive biological behaviors. Anthracyclines (Doxorubicin) based treatment regime were reported to cause cardiotoxicity by increasing intramyocardial free radical production, lipid alterations and decreasing antioxidant level. Oxidative stress involving cellular reactive oxygen species (ROS) production is widely accepted mechanism but the molecular basis of chemotherapy induced organ toxicity remains highly controversial. An increased rate of metabolism and oxidative stress results rapid turnover of cancer cells that modulates the enzyme level in blood circulation. Serum LDH and GGT level correlates with tumor burden, metastatic character of BC and intensity of organtoxicity. The aim of our study is to evaluate the serum level of LDH and GGT in BC patients receiving chemotherapy and correlate these enzyme levels with different stages of BC. A total number of 150 subjects were included in the study, comprising 90 histopathologically confirmed 24 to 76 years aged patients of different breast cancer stages, receiving at least 3 cycles of 5-Fluorouracil, Adriamycin and Cyclophosphamide (FAC) chemotherapy. Sixty age- matched healthy women were enrolled as controls. Blood samples from each individual was collected after informed consent and analysed for serum LDH and GGT levels using standard biochemical methods. Data were analyzed using student's paired 't' test, Pearson correlation test and ANOVA. Serum LDH and GGT levels were significantly (p<0.001) increased in BC patients as compared to control group. When all 4 stages of BC were compared to control group, LDH and GGT showed steady and progressive increase in the activities of these enzymes from stage I-IV. The study concludes that serum LDH and GGT may be sensitive, specific and cost effective biomarkers in early diagnosis of breast cancer, assessing cancer prognosis and response to treatment.
Botanical Survey in Moyo Island, West Nusa Tenggara, Indonesia: Inventory of Flora Collection at Forest Trimanto, Trimanto; Siahaan, Febrina Artauli
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.12

Abstract

Nusa Tenggara consists of some small islands, one of them is Moyo Island. The diversity of plant species in this island is not really known for certain. This research was determined to observe the diversity of plant spe-cies in Moyo Island forest. The research was conducted in April 2013 by using floristic analysis method. The characteristic of Moyo Island forest is lowland evergreen rain forest. The results showed there were 60 tree species recorded in Moyo Island forest. There were many fruiting trees and seedling from the trees which show healthy growth, indicated that the plant regeneration in this forest is went well. The diversity of Pteri-dophytes and orchids were not high. Epiphytic fern which often found in the forest were Drynaria quersifolia and Platycerium bifurcatum and terrestrial orchid that dominated in the forest was Nervilia aragoana. Tuber plant was often found in this forest and grew prolifically were Tacca, Dioscorea and Amorphophallus. In coastal area lived a population of Pandanus tectorius. There were three new record plants found. The first was epiphytic orchid: Pteroceras javanica, the second was the epiphytic plant: Hoya verticillata and wild tuber plant: Tacca leontopetaloides.
Role of Antibody Anti-AGE in the Expression of Nephrin and RAGE on Primary Glomerulus Cell Exposed with AGE Salam, Rudy; Lyrawati, Diana; Samsu, Nur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.03

Abstract

Upregulation of nephrin expression occurs at the early stage of nephropathy and decrease in the period 6 months on hyperglycaemic condition. Nephrin is associated with the initial stage of the loss of the permeability barrier in diabetic nephropathy. Interaction AGE-RAGE increases angiotensin II on Renin Angiotensin-Aldosterone System (RAAS) and activation of protein kinase c (PKC) which induce alterations in nephrin mRNA expression. Alterations of nephrin expression induces transformation of slit membrane structure and the permeability changes at the glomerular filtration barrier. Anti-AGE vaccination once may cause the changes of nephrin and RAGE expression and can prevent progression of diabetic nephropathy. This study used primary glomerulus cell culture obtained from renal of Wistar mice aged 3 months, weighting 200-300 grams that consist of negative control group that exposed to BSA 100 µg/ml, positive control group that exposed to AGE-BSA 100 µg/ml, treatment group 1 that exposed to polyclonal anti-AGE 5 µg/ml and AGE-BSA 100 µg/ml and treatment group 2 that exposed to monoclonal antibody anti-CML 5 µg/ml and AGE-BSA 100 µg/ml. Paired t-test with a 0.05 level of confidence results showed that there were significant differences in level of RAGE expression among experimental groups with control groups. Administration of polyclonal antibody decreased RAGE expression among negative control (p=0.188). but not in positive control (p=0.000). In contrast to monoclonal anti-AGE antibody, RAGE expression did not differ significantly compared to negative control but significant than positive control. Administration of monoclonal anti-AGE antibody inhibited increasing of nephrin expression compared to negative and positive control (p=0.73; 0.125). In conclusion, this study suggested that administration of polyclonal and monoclonal anti-AGE antibody could inhibit increasing of RAGE and nephrin expression in glomerulus primary culture that exposed to AGE which is expected to prevent the progression of diabetic nephropaty.
The Level of Egg Sterility and Mosquitoes Age After The Release of Sterile Insect Technique (SIT) in Ngaliyan Semarang Sutiningsih, Dwi; Rahayu, Ali; Puspitasari, Devi
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.08

Abstract

Dengue control efforts have not provided adequate results. Therefore we need other measures like sterile in-sect Technique (SIT). This study aimed to observe the level of egg sterility and age of Aedes aegypti mosqui-toes after using SIT in Ngaliyan Semarang. The method of this research is a descriptive study with a cross-sectional design. A sample of the mosquitoes eggs are all the mosquitoes eggs on the paper trap in every house of RW2 population in Ngaliyan village. These samples are the result of the fertilization of male and female mosquitoes in nature. Data analysis using Wilcoxon sign test. The result showed that the average of egg sterility percentage in mosquitoes after using SIT indoors was 73.65% and the average of egg sterility per-centage outdoors was 69.08%. Wilcoxon test showed significant differences in average age of mosquitoes egg (p < 0.05), males pupae (p < 0.05), females pupae (p < 0.05), and adult female mosquitoes (p < 0.05), before and after SIT. It can conclude from this research that the SIT release in Ngaliyan village Semarang city could increase the level of sterility mosquito’s eggs and a decrease of the age of Ae. aegypti mosquitoes in this area.

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