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INDONESIA
Journal of Agricultural Science and Biotechnology
Published by Universitas Udayana
ISSN : 23020113     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara luas.
Arjuna Subject : -
Articles 4 Documents
 1 
Search results for , issue "Vol 6 No 1 (2017)" : 4 Documents clear
PENGARUH PENGGUNAAN SAMPAH ORGANIK DAN CACING TANAH (Lumbricus rubellus) TERHADAP SIFAT KIMIA DAN BIOLOGI PUPUK KASCING Ditamulia Slamet Utama; I Made Sudana; Ni Luh Kartini
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The use of agricultural land without organik fertilizers can decrease soil organik matter and decrease soil productivity. Production of organik fertilizer with natural composting process will be take a long time. The addition of earthworm (Lumbricusrubellus) on organik materials can accelerate the composting process. Vermicompost has a lot of plant growth hormones, beneficial microbes, and plant nutrients. Research purposes were to determine the effectiveness of earthworms to decompose the organic matter, to determine the weight earthworms are able to accelerate the decomposition of organic waste into fertilizer, and to determine the biological and chemical fertilizers produced from organic waste with earthworms compared without earthworms. The research method used a randomized block design 2 factorial. The variables measured were the weight of earthworms, the decomposition effectiveness, quality of fertilizers and fertilizer application in mustard. The data was analyzed by analysis of variance, if there was a significant interaction effect of the variable followed by Duncan's multiple range test, and if the only real effect of a single factor followed by LSD test.The result show that the earthworm effective in the decomposition of organic waste into vermicompost fertilizer. Increasingly the weight of the earthworm the process of decomposition organic material into vermicompost fertilizer more quickly,the best treatment for the decomposition of organic matter was C3B1, C3B2, and C3B3. Biological and chemical character of fertilizer produced from organic waste with earthworms is better than without earthworms. The best treatment was C1B3.
KARAKTERISTIK MOLEKULER DAN FILOGENI LALAT BUAH Bactrocera occipitalis (Diptera:Tephritidae) DARI TARAKAN BERDASARKAN SEKUEN NUKLEOTIDA GEN COI Dwi Martiningsia; I Nyoman Wijaya; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Bactrocera occipitalis is one species of group B. dorsalis complex. The main host of B. occipitalis is mango (Mangifera indica), guava (Psidium guajava), orange (Citrus sp), sapodilla (Achras zapota) and star fruit (carambola Averrhoa). Region of distribution B. occipitalis are Philippines, East Malaysia (Sabah), Brunei, Indonesia (Kalimantan). B. dorsalis complex has similar morphological and have a less distinctive character for taxonomic identification at immature life stages, it will be an obstacle for the export of fruit and vegetables from Indonesia related to quarantine the country of destination, and DNA-based barcode can solve this problem. Molecular Identification B. occipitalis Tarakan successfully amplified at 642 bp with COI gene. Based on sequence homology COI B. occipitalis Tarakan has a high of similarity with B. occipitalis Philippines is 99.4% -100%. The phylogenetic analysis of DNA sequences and genetic distance based on COI gene sequences showing too genetic closeness with B. occipitalis Philippines.
IDENTIFIKASI SPESIES POTYVIRUS PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI (Capsicum frutescens L.) MELALUI SIKUEN NUKLEOTIDA GEN Coat Protein I Gede Rian Pramarta; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Chili peppers (Capsicum frutescens L.) is one of the important vegetable in the world and one of the leading horticultural commodities in Indonesia. Pepper plants grown in all provinces in Indonesia and got priority for development because it has great potential economic value. These researches was conducted in order to determine the type of infecting Potyvirus on chili and know the proximity nucleotide sequence of Potyvirus infecting chili crop in Kerta village, Payangan, Gianyar, Bali, with the kind of Potyvirus in other areas. This study use RT-PCR technique to detect plant viruses and continued with tracing sequence coat protein gene nucleotide to its proximity to some of the isolates analyzed from GeneBank. The results showed that the infecting Potyvirus chili crop is Chili veinal motle virus species (ChiVMV) with size of approximately 900 bp DNA band, and based on phylogenetic analysis of coat protein gene nucleotide sequence, PayanganIndo isolate had high similarity (93%) and small genetic distance (0.041) with isolate of origin Thailand state.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN LEGUMINOSAE UNTUK MENGENDALIKAN JAMUR Sclerotium rolfsii PENYEBAB PENYAKIT REBAH KECAMBAH PADA TANAMAN KEDELAI Komang Adi Mahartha; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Damping off disease caused by Sclerotium rolfsii is an important disease of soybean, because soybean plant will die after attacked by pathogen. This pathogen can survive for a long time in the soil by sclerotia form. Some of rhizobacteria isolated from the rhizospheres of Leguminosae plants were known could control soil-borne pathogens. This study aims to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to suppress the growth of S. rolfsii. Total of 11 isolates of 112 isolates rhizobacteria that could be isolated from Leguminosae plants had antifungal activity against S. rolfsii. Inhibition test of 11 isolates of rhizobacteria against S. rolfsii showed that three isolates had the highest ability to inhibit the growth of S. rolfsii namely isolates KtD1, KtD6, and KtB3. This result suggested that three isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of damping off disease on soybean.

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